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jcm4620
Stranger


Registered: 05/26/19
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i got actual loops also but i honestly prefer my sterile swab packs. i bought 2 boxes of 200 for 10$ each and that was over 2 yrs ago and i still have almost a whole box left. i even gave several away these will last me at least another year. plus i just prefer the length and the feel of the firm tip on the agar. i cant stand regular soft q tips but the firm 6 inch ones with the wooden stick i like better than the loop
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jcm4620
Stranger


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Re: Official Copelandia/Panaeolus Thread [Re: jcm4620]
#26873469 - 08/10/20 02:23 PM (3 years, 5 months ago) |
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im thinkn these peace river trays should end up being ok🤞🤞 il know more in the next day or 2 but so far in 1 day these things are shooting up quick
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mushboy
modboy



Registered: 04/24/05
Posts: 32,276
Loc: where?
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Re: Official Copelandia/Panaeolus Thread [Re: c10h12n2o]
#26873471 - 08/10/20 02:23 PM (3 years, 5 months ago) |
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Quote:
c10h12n2o said:
Quote:
mushboy said: Pan lc skin

Yeah but does it taste clean? Thats the real question haha
 I'll let you know
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c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
Loc: the abyss
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Re: Official Copelandia/Panaeolus Thread [Re: mushboy]
#26873485 - 08/10/20 02:35 PM (3 years, 5 months ago) |
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I love loops. Especially for doing that serial streaking pattern i like
Gives you tons of colonies to choose from, progressively cleaner colonies, and progressively less strain groupings per colony (gif in my sig)

Also you can use loops for loading slants, swabs not so much
Even when i use a swab (like pe spore swab), i still use a loop. I just do the first zone with the swab then use the loop for the other 3 zones
Nothing wrong with doin it the swab way though, i just like the advantages of the loop
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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jcm4620
Stranger


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Re: Official Copelandia/Panaeolus Thread [Re: mushboy]
#26873531 - 08/10/20 03:03 PM (3 years, 5 months ago) |
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oh for sure. i have both for that reason
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Tweeq
Tweeq of Nature


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Re: Official Copelandia/Panaeolus Thread [Re: jcm4620]
#26873625 - 08/10/20 04:10 PM (3 years, 5 months ago) |
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I seem to be the only one who just drops the spores on to agar from print with a sterile tool.. which, btw, works pretry good
Do i need to get loops? Will that be even more clean?
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rido



Registered: 05/13/19
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Loc: Texas
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Re: Official Copelandia/Panaeolus Thread [Re: Tweeq]
#26873721 - 08/10/20 05:09 PM (3 years, 5 months ago) |
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Quote:
Tweeq said: I seem to be the only one who just drops the spores on to agar from print with a sterile tool.. which, btw, works pretry good
Do i need to get loops? Will that be even more clean?
That works fine, but spreading/streaking helps them not be all lumped together. Loops are better suited to streaking either a liquid culture or spore solution, while a sterile swab works well for streaking from a dry print. Clean is pretty straightforward in this hobby. There are degrees of cleanliness and sterility, sure, but all we typically know or care about is a binary yes/no. Either your process was clean enough, or it wasn't. If you're not having recurrent contam issues I wouldn't worry about it.
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rido



Registered: 05/13/19
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Re: Official Copelandia/Panaeolus Thread [Re: rido]
#26873727 - 08/10/20 05:17 PM (3 years, 5 months ago) |
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These are both T2 plates from LTC's Rainbow Eucalyptus. Do these look alright to inoculate some LC?
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Phrontist
Determined



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Re: Official Copelandia/Panaeolus Thread [Re: Tweeq]
#26873740 - 08/10/20 05:25 PM (3 years, 5 months ago) |
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Quote:
Tweeq said: I seem to be the only one who just drops the spores on to agar from print with a sterile tool.. which, btw, works pretry good
Do i need to get loops? Will that be even more clean?
What a coincidence. I just put spores to agar for the first time, and all I did was scrape a bit of the print loose with a flamed Exacto blade, then drop them onto the agar surface. Figured I could add a little distilled water later if it was necessary.
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Smoothcat
Renegade-master



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Re: Official Copelandia/Panaeolus Thread [Re: Phrontist] 1
#26873762 - 08/10/20 05:44 PM (3 years, 5 months ago) |
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Pan cyan wild coast & blue springs 5 days in, fair to say they’re chugging along nicely
-------------------- Back once again with the ill behaviour Links I like
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c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
Loc: the abyss
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Re: Official Copelandia/Panaeolus Thread [Re: Smoothcat]
#26873792 - 08/10/20 06:04 PM (3 years, 5 months ago) |
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Depends what you are trying to do
I mostly use loop for spore prints. Heat loop, cool in agar, scrape spores, streak section 1, flame, streak section 2, flame, repeat
Swab works fine too tho, its just waaaay more strain groupings per colony (not necessarily a bad thing). Streaking w a loop is really handy with a dirty print
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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Asura
Cyantist


Registered: 08/01/11
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Re: Official Copelandia/Panaeolus Thread [Re: c10h12n2o]
#26873844 - 08/10/20 06:27 PM (3 years, 5 months ago) |
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jcm, these jambo's are insane at day 3.


Very aggressive. This isn't overlay. The myc actually looks really nice and fluffy. I've never really had this before so I have no idea what's going to happen.
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Celestialexplorer1



Registered: 03/25/20
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Re: Official Copelandia/Panaeolus Thread [Re: Asura] 1
#26873881 - 08/10/20 06:49 PM (3 years, 5 months ago) |
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Outdoor aussies that will never mature because of slugs
 Some miniature bisporus.
And this is how my luck goes.
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 To spend just one moment in eternity
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jcm4620
Stranger


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Re: Official Copelandia/Panaeolus Thread [Re: Asura]
#26873898 - 08/10/20 06:59 PM (3 years, 5 months ago) |
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Quote:
Asura said: jcm, these jambo's are insane at day 3.


Very aggressive. This isn't overlay. The myc actually looks really nice and fluffy. I've never really had this before so I have no idea what's going to happen.
just curious do u have coir in ue casing??
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Asura
Cyantist


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Re: Official Copelandia/Panaeolus Thread [Re: jcm4620]
#26873903 - 08/10/20 07:02 PM (3 years, 5 months ago) |
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Coir in my casing?
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badreligion2good
Uncertain



Registered: 02/21/06
Posts: 888
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Is the miniature size thought to be more genetic or environmental? I'm noticing the "second flush" on the jambos are behaving differently based on how saturated I have the substrate. The more saturated produced smaller dense fruits, whereas the dried ones produced taller ones. One tray that was producing tall ones now appears to be producing mini-looking ones, and I think it might have to do with the amount of moisture in the substrate. Just a theory based on my limited experience.
Anyone else have ideas as to how the same tray can go from producing tall ones to minis?
-------------------- All I know is that I dont know. Row, row, row, you boat, gently down the stream. Merrily, merrily, merrily, merrily, life is but a dream.
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Blue Helix
bold hand


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Does anyone know what "rain euc" or "rain eue" might be? I got a print labeled that, but I don't know who sent it. I don't know what it is, but it looks like a pan cyan print of some sort.
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Blue Helix
bold hand


Registered: 02/02/03
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Last seen: 6 months, 18 days
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Re: Official Copelandia/Panaeolus Thread [Re: Asura]
#26874045 - 08/10/20 08:30 PM (3 years, 5 months ago) |
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Quote:
Asura said: Coir in my casing?

Asura it looks like you are using coco coir in your casing. That's exactly what the mycelium looks like when you use coco coir in cubensis for example:
 
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A.k.a
Stranger



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Re: Official Copelandia/Panaeolus Thread [Re: Blue Helix]
#26874083 - 08/10/20 08:51 PM (3 years, 5 months ago) |
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Rainbow eucalyptus. I just got that too.
My wild coast flushes got drastically shorter each one too.
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LAGM2020     
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Celestialexplorer1



Registered: 03/25/20
Posts: 1,803
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Quote:
badreligion2good said: Is the miniature size thought to be more genetic or environmental?
It is genetic I’m sure of it.
--------------------
 To spend just one moment in eternity
Edited by Celestialexplorer1 (08/10/20 08:56 PM)
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