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Offlinejcm4620
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Re: Official Copelandia/Panaeolus Thread [Re: jcm4620]
    #26862928 - 08/04/20 04:03 PM (3 years, 5 months ago)

here c 10 these are all transfers from the tissue plate of the peace river clone im runnin right now minus the 2 that i have going. il still fruit all these out also.



you can see they still go every which way and thats ok. i dont have pics of the tissue plate of these but i do still have it in the fridge so il use these tissue plate pics as an example. stry bout shitty saran wrap and tape i was out of parafilm and the generic saran wouldnt cling so i had to tape it lol



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Invisiblealaskappalachian
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Re: Official Copelandia/Panaeolus Thread [Re: jcm4620]
    #26862939 - 08/04/20 04:10 PM (3 years, 5 months ago)

Quote:

jcm4620 said:
i never like to go more than say t3 from the streak plate just cuz i figure if i aint got a good looking strong culture by then its prolly not worth using. but i dont even really care about sectoring and having a perfect circle culture. im after clones period. so my goal is to get a good looking clean strong culture on grain as fast as possible while i have the largest potential gene pool as possible. if u spend to much time making transfers your eventually just gnna most likely end up transfering away from somethin you may want that is unless you intend on fruiting each one out but fuck all that madness . just cuz it looks perfectly round and pretty dont mean its good either. as for clones they are almost never perfectly round and will usually grow every which way and thats why i take as many transfers as possible from my tissue plates and then fruit them all out and toss the shit ones while keeping the good ones. thats pretty much what i do




^^^ Guess who's learning this the hard way with pans this week?  *points to self and pulls trigger*.  Thought this would be a cakewalk like most other myco work and it's just been a shit show.  So many weak plates and every time I get a half decent one (keeping to the less-transfers-is- more theory) it expresses white mold or goes green on grain.  I'm oofed out.  Working with pans is going to make me a better mycologist, I can tell already...  Starting from scratch tonight after tossing my last plate...:facepalm3: This thread has been INVALUABLE.


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Offlinejcm4620
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Re: Official Copelandia/Panaeolus Thread [Re: alaskappalachian] * 1
    #26862955 - 08/04/20 04:18 PM (3 years, 5 months ago)

that sux man. it takes a bit to get the eye down for what to grab but watch your streak plates like a hawk and start making transfers as soon as u start seeing actual germination points. il take those the very second i see them that way i can split shit all up making it easier when you start atcually getting myc growth. but once u know what to take u should have no problem at all with getting a clean culture by t2 or 3


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Invisiblebadreligion2good
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Re: Official Copelandia/Panaeolus Thread [Re: A.k.a]
    #26862975 - 08/04/20 04:25 PM (3 years, 5 months ago)



My friend sent me a picture of her first Pan Cyan pin ever. Jamaican me crazy with all those hyphal knots...

Method:
- Swabbed to agar.
- Clean T1 to 1 qt RGS
- Dried aged horse poo, rehydrated, and pasteurized in the oven at 150-170 for 2 hours
- Spawned 1 qt RGS spawn to roughly 2 quarts of pasteurized hpoo and placed in trays 1-2 inches deep. in a glovebox. Then wrapped with aluminum and left to colonize bulk for 5 days in SAB.
- Cased with thinnest possible layer of 50-50 mix on day 5 from spawn to bulk
- Placed in SGFC and misted/fanned 3-4 times a day for 3 days.
- Day 8 from spawn to bulk: Stopped misting straight into the SGFC, continued fanning just as frequently and misting outside of the SGFC.
- Day 9 - First pin in a tiny container made and lots of hyphal knots throughout all of the pans (a 5x5 and a few small circular food containers of varying sizes that were upcycled.

She says that it seems pretty simple if you understand the basic process...

Any estimates on time from hyphal knots to opening caps? She is dying to know.

Also, she says thanks for sharing your thoughts and ideas. She says that without these threads, this would be a lot less straight-forward.


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All I know is that I dont know.

Row, row, row, you boat, gently down the stream.
Merrily, merrily, merrily, merrily, life is but a dream.


Edited by badreligion2good (08/04/20 04:30 PM)


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OfflineJakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: jcm4620]
    #26862978 - 08/04/20 04:28 PM (3 years, 5 months ago)

Why do you think bulk bags will make it bigger do you mean bags of grain or just inoculating bulk with LC? Pans do fine on grain you just have to learn their preferred moisture content but I feel I get better consistent results running through grain then just going directly to bulk with LC. Right now my failure rate is probably right around 25% in the winter time I can get it down to 10% through 5% (approx) I’ve now been able to tighten up and my success rate has improved vastly over my last two runs but I am constantly making changes with the seasons materials strains etc. I am rebuilding my fruiting chamber simply because it’s over a year old and my contamination rates once placed end to it are higher than they have ever been so I have two brand new tents coming I will also deep clean the fogger and replace the hosing


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Edited by Jakeoncid419 (08/04/20 04:43 PM)


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Offlinejcm4620
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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
    #26862988 - 08/04/20 04:35 PM (3 years, 5 months ago)

i too have great results with using grain


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OfflineCelestialexplorer1
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Re: Official Copelandia/Panaeolus Thread [Re: jcm4620]
    #26862997 - 08/04/20 04:39 PM (3 years, 5 months ago)


These are some wild pan cyan cultures a couple
Days before putting to grain.

It’s not that I’m getting bad results but the myc just looks healthier on the bulk mix then it did on agar or grain. I don’t know maybe it’s the wild strains because the jams I did and wild coast all looked good on grain. I’ll take a picture difference when I get home of the wild strains grain/bulk bags.


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To spend just one moment in eternity


Edited by Celestialexplorer1 (08/04/20 04:44 PM)


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Offlinejcm4620
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Re: Official Copelandia/Panaeolus Thread [Re: Celestialexplorer1]
    #26863004 - 08/04/20 04:43 PM (3 years, 5 months ago)

i wouldnt put those to grain


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Offlinejcm4620
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Re: Official Copelandia/Panaeolus Thread [Re: jcm4620]
    #26863009 - 08/04/20 04:44 PM (3 years, 5 months ago)

id maybe take a cpl transfers in a few spots but that growth is usually the growth i have problems with not recovering or stalling out


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Offlinejcm4620
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Re: Official Copelandia/Panaeolus Thread [Re: jcm4620]
    #26863016 - 08/04/20 04:49 PM (3 years, 5 months ago)

like i said i personally wouldnt use these at all but if i was gnna try to maybe get somethin out of them id try these spots but even then i still dont like them for something id put to grain.



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OfflineCelestialexplorer1
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Re: Official Copelandia/Panaeolus Thread [Re: jcm4620]
    #26863018 - 08/04/20 04:50 PM (3 years, 5 months ago)

I took those pics two days after the transfer and they grew out pretty quick. I didn’t take any pics before putting to grain but they looked a lot better. They were the most vigorous and quick recovering cultures I had of those particular strains. But I don’t know I haven’t been doing this for as long as you have your probably right.


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Offlinejcm4620
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Re: Official Copelandia/Panaeolus Thread [Re: Celestialexplorer1]
    #26863022 - 08/04/20 04:54 PM (3 years, 5 months ago)

i look more for stuff like this


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Offlinejcm4620
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Re: Official Copelandia/Panaeolus Thread [Re: jcm4620]
    #26863025 - 08/04/20 04:56 PM (3 years, 5 months ago)

but if they r on grain and looking good thats all that matters hope they work out for ya👍😃


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OfflineCelestialexplorer1
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Re: Official Copelandia/Panaeolus Thread [Re: jcm4620]
    #26863075 - 08/04/20 05:22 PM (3 years, 5 months ago)


And that’s exactly what I look for these are plates that the transfers I put to grain came from and to me  these look
A lot like your picture


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Offlinejcm4620
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Re: Official Copelandia/Panaeolus Thread [Re: Celestialexplorer1]
    #26863111 - 08/04/20 05:39 PM (3 years, 5 months ago)

ya most those look good


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OfflineCelestialexplorer1
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Re: Official Copelandia/Panaeolus Thread [Re: jcm4620]
    #26863145 - 08/04/20 05:54 PM (3 years, 5 months ago)

Well I spawned almost every single one to separate grain so let’s hope. Lol


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OfflineQuagmire75
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Re: Official Copelandia/Panaeolus Thread [Re: Celestialexplorer1] * 1
    #26863315 - 08/04/20 07:29 PM (3 years, 5 months ago)

Hey guys, I'm glad to say that my third attempt at cultivating pans was a success. It wasn't a panopy but I still got alot of fruits, by my standards anyway 😂. I had several trays in the GH but the one pictured was the best. These are wild coast (thanks again psilo!). I want to give back to the community that has helped me so much so I made some prints each "print" has prints from two caps.*note* these will need to be cleaned up on agar, my grow room is in an old barn (not sterile by no means). So if any of you guys are interested I have eleven prints to give away. Just sent me a pm and I'll get them sent out. Thanks again. Especially jcm, Jake, and asura.


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Offlinejcm4620
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Re: Official Copelandia/Panaeolus Thread [Re: Quagmire75]
    #26863406 - 08/04/20 08:15 PM (3 years, 5 months ago)

congrats👍👍👍😃


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Invisiblec10h12n2o
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Re: Official Copelandia/Panaeolus Thread [Re: jcm4620]
    #26863450 - 08/04/20 08:42 PM (3 years, 5 months ago)

Yall said you do bags with filter side in and aluminum foil, whys that? Ive always done filter out

Do yall preseal when you plan to inoc w lc? Ive always been pretty lucky with it. But then a few days ago i had 2 bags blow that werent even presealed! Think i vented too long


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"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
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OfflineCelestialexplorer1
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Re: Official Copelandia/Panaeolus Thread [Re: c10h12n2o]
    #26863461 - 08/04/20 08:50 PM (3 years, 5 months ago)

So how wet is too wet. Like you want it glistened by but covered and you don’t want it just beads on the surface like a cube tray?


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To spend just one moment in eternity


Edited by Celestialexplorer1 (08/04/20 08:50 PM)


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