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InvisibleModularMind
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Re: Official Copelandia/Panaeolus Thread [Re: jcm4620]
    #26696558 - 05/26/20 09:09 AM (3 years, 8 months ago)

The internal temperature will eventually equalize to the set temperature. The advantage of the sous vide is the ability to maintain the exact temperature once achieved. That commercial grade unit probably does quite well.


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Offlinejcm4620
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Re: Official Copelandia/Panaeolus Thread [Re: ModularMind]
    #26696574 - 05/26/20 09:19 AM (3 years, 8 months ago)

il have to look into those maybe il find one at a bargain


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OfflineGrimsweeper
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Re: Official Copelandia/Panaeolus Thread [Re: ModularMind]
    #26696584 - 05/26/20 09:24 AM (3 years, 8 months ago)

I used a sous-vide for a long time before I got a Precision slow cooker with a probe. Still use it occasionally if I only need a quart or two of casing.


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Re: Official Copelandia/Panaeolus Thread [Re: jcm4620]
    #26696588 - 05/26/20 09:26 AM (3 years, 8 months ago)

Please can someone help me identify whether this is Pan Cyan mycelium or some contaminant?



Jar is something like two weeks old at this point. Mix is mostly coir with a little BRF and dried hpoo (rehydrated). Not many other signs of life.


Edited by BlueWonder (05/26/20 09:28 AM)


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Offlinejcm4620
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Re: Official Copelandia/Panaeolus Thread [Re: BlueWonder]
    #26696593 - 05/26/20 09:30 AM (3 years, 8 months ago)

if thats all there is after 2 weeks my guess is its not. pans move pretty damn fast but either way the pic is horrible and cant really tell what it is


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Edited by jcm4620 (05/26/20 09:30 AM)


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OfflineA.k.aM
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Re: Official Copelandia/Panaeolus Thread [Re: jcm4620]
    #26696668 - 05/26/20 10:13 AM (3 years, 8 months ago)

Yeah it looks like myc to me but either way it’s no good.

Like he said pans are fast two weeks should be like harvesting second flush.


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OfflineBlueWonder
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Re: Official Copelandia/Panaeolus Thread [Re: A.k.a]
    #26696868 - 05/26/20 11:41 AM (3 years, 8 months ago)

Thanks everyone.

I've just never had any luck with them. I can get cubes to germinate without too much trouble, but every time I've tried with pans, I can barely get them to form mycelium at all.

Sorry about the bad picture, difficult to capture a good one.

Suggestions about what to do? I don't really want to throw it out in case it is really mycelium. Just leave it? Transfer it to something open air?


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Offlinejcm4620
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Re: Official Copelandia/Panaeolus Thread [Re: BlueWonder]
    #26696923 - 05/26/20 12:12 PM (3 years, 8 months ago)

r u using agar???


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Offlinemullugh
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Re: Official Copelandia/Panaeolus Thread [Re: jcm4620]
    #26696957 - 05/26/20 12:26 PM (3 years, 8 months ago)

Quote:

jcm4620 said:
perlite aint gnna really b enough and misting pins will kill them. they r fucking picky as shit about that temp between 77-81 and that super high humidity i mean high to where if u were to have fruits in there they would bave beads of moisture on them just from the saturation of the air around them. but u also need super high fae and periods of evaporation and re saturation. its a fine line man and every environment seems to require different tweeking until you can get that good balance of everything. atleast based on my experience with them. i also tried just sgfc and hc with perlite and soon as they got an inch above the casing they would die. so if ur serious about growin them ur better off doing it rite from the begining by setting up a proper fc for them there is a link in my sig that has my method and links to jakes and asuras write ups as well




Good post imho


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Re: Official Copelandia/Panaeolus Thread [Re: BlueWonder]
    #26696965 - 05/26/20 12:30 PM (3 years, 8 months ago)

Quote:

BlueWonder said:
Please can someone help me identify whether this is Pan Cyan mycelium or some contaminant?



Jar is something like two weeks old at this point. Mix is mostly coir with a little BRF and dried hpoo (rehydrated). Not many other signs of life.




I found that grains with a tiny bit of manure mixed in worked best for me for spawn (and I mean a tiny bit). Then spawn onto manure/verm/gypsum/straw (I cut dry straw in you the mix as the manure is wet enough to hydrate it).
I pasteurize it in oven bags for 2 hours in the oven.

Hope that helps.


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Re: Official Copelandia/Panaeolus Thread [Re: jcm4620]
    #26696968 - 05/26/20 12:32 PM (3 years, 8 months ago)

Quote:

jcm4620 said:
ya but how do u know the internal temp is correct cuz il promise u that just cuz it says 165 on the readout dont mean its 165 in the middle of the bags. i know when im doin my shit the temp around it and the temp inside of it are 2 totally different things





I usually have a probe stuck in one one of the bags and I’ll rotate it but it broke last month - I never replaced it, haven’t felt the need too -

I just leave the bags in over night and they’re good.

I’d check them out - they’re great for pasteurizing /  I can probably do 60#s at a time in there .

I also use it for keeping my “tree bark “ vessel warm when  pulling dreams .


Edited by thenagual (05/26/20 12:34 PM)


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Offlinethenagual
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Re: Official Copelandia/Panaeolus Thread [Re: BlueWonder]
    #26696977 - 05/26/20 12:36 PM (3 years, 8 months ago)

Quote:

BlueWonder said:
Thanks everyone.

I've just never had any luck with them. I can get cubes to germinate without too much trouble, but every time I've tried with pans, I can barely get them to form mycelium at all.

Sorry about the bad picture, difficult to capture a good one.

Suggestions about what to do? I don't really want to throw it out in case it is really mycelium. Just leave it? Transfer it to something open air?






Also , if using agar - try different recipes .

I had trouble with standard MEA recipe for a while with pans .

I ended up germinating on potato then reverted back to MEA once culture germinated .


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Offlinejcm4620
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Re: Official Copelandia/Panaeolus Thread [Re: thenagual]
    #26697029 - 05/26/20 12:58 PM (3 years, 8 months ago)

i know ive said it a million times lol  but if agar is givin ya trouble you can also knock up a small grain jar and pluck kernals to plates to get what your looking for. thats what i do and it works like a charm. but its rare to have to do that with a fairly fresh print. i only use regular ol mea and i hardly ever have any issue getting germination when working from prints under a year old it seems.


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Edited by jcm4620 (05/26/20 01:00 PM)


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OfflineJakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: jcm4620]
    #26697071 - 05/26/20 01:20 PM (3 years, 8 months ago)

I’ve said it before I’ll say it again pans really respond well to mycological peptone in their agar


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Re: Official Copelandia/Panaeolus Thread [Re: BlueWonder]
    #26697088 - 05/26/20 01:28 PM (3 years, 8 months ago)

Never used agar - this was straight spores into coir, hpoo and brf. Also tried spores straight into grain without success.

I'll leave it for a few more days. Perhaps I'll mix it into a grain jar with a little hpoo as you said. Just worried about getting contams into the myc that I do have - I mean, if I leave it alone, it is growing but at least it shows no sign of contams. Nothing obvious like discoloured wet spots.


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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
    #26697097 - 05/26/20 01:32 PM (3 years, 8 months ago)

Quote:

Jakeoncid419 said:
I’ve said it before I’ll say it again pans really respond well to mycological peptone in their agar




https://www.neogen.com/solutions/microbiology/mycological-peptone/

Thats about the cheapest I could find it, do you think this would do the trick? I'm using soy peptone now with good results.


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Offlinejcm4620
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Re: Official Copelandia/Panaeolus Thread [Re: Colorblind-Myc]
    #26697107 - 05/26/20 01:39 PM (3 years, 8 months ago)

ive never tried that before jake is that something that is part of your go to recipe or is it just somethin you will add if having a difficult time?


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InvisibleCosmicGiggle
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Re: Official Copelandia/Panaeolus Thread [Re: jcm4620]
    #26697163 - 05/26/20 02:11 PM (3 years, 8 months ago)

Quote:

jcm4620 said:
ive never tried that before jake is that something that is part of your go to recipe or is it just somethin you will add if having a difficult time?




It’s in the recipe he lists in his Pan tek thread. Cant speak for him but I would guess that means he uses it regularly, although he does mention that recipe being for stubborn cultures. But I’ve seen him mention that he adds peptone many times throughout this thread


Quote:

jcm4620 said:
well i just know what ive experienced and it seems like the core temp was always different but hey if its workn for ya thats all that matters. i know i have used slow cookers b4 and i had to keep playin with the temp setting to get the core temp rite and then when i would the outter temps would b too high. so in the end i just went back to using jars on the stove




regarding the sous vide, if you’re interested I just bought this one Sous vide on amazon for $54 although it looks like it went up a little in price.
Good reviews. I only used it once and fucked my shit up (not the Sous vide’s fault) but I put it in a big pot of water and set it to 162F degrees for 12 hours (per the JOC tek, although he uses a slow cooker) and it got the entire pot of water up to temperature in about 2 min. If you pasteurize your bulk overnight like JOC, the internal temp will undoubtedly reach the desired temp. I didn’t even bother throwing a thermometer in mine, but then again I fucked up and sealed my bags air tight so I wouldn’t have been able to anyways :rolleyes:

Also worth noting, with the Sous vide, a good one will not fluctuate in temperature more than 1F degree during its entire cooking cycle so you should not have to monitor it at all unless you’re just checking to see if the sub has gotten up to desired temperature yet. if you’re not letting it pasteurize overnight, that is


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Edited by CosmicGiggle (05/26/20 02:30 PM)


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Offlinejcm4620
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Re: Official Copelandia/Panaeolus Thread [Re: CosmicGiggle]
    #26697182 - 05/26/20 02:21 PM (3 years, 8 months ago)

i may look into it. rite now ive still got enough bagd sub and casing for 28 of my trays worth in the deep freezer so im gnna be set for a while yet.


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InvisibleCosmicGiggle
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Re: Official Copelandia/Panaeolus Thread [Re: jcm4620]
    #26697201 - 05/26/20 02:34 PM (3 years, 8 months ago)

Quote:

jcm4620 said:
i may look into it. rite now ive still got enough bagd sub and casing for 28 of my trays worth in the deep freezer so im gnna be set for a while yet.




Woah...you can freeze your bulk after pasteurizing and use it later? Doubt the ol bag would let me keep horse shit resting on top of our frozen pizzas but that’s an interesting note to hold onto. I’ve read multiple books worth of threads/posts on here and still only scratching the surface on everything there is to know


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