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A.k.a
Stranger



Registered: 10/27/19
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Are you sure that bacterial one isn’t cubes?
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Asura
Cyantist


Registered: 08/01/11
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Those dense white masses are something I never want to see in a tray. It happens and it doesn't mean all is lost, but it hasn't ever been a good sign for me personally.
Once I lay a tray, I really don't like to keep it in recovery for longer than 5 days. So if it's ready it's ready and if not, I would put it in fruiting. Again, that's just my preference. But keep in mind, I am putting fully colonized sub in trays. If you are spawning directly to trays you could probably go a little longer.
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jcm4620
Stranger


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Re: Official Copelandia/Panaeolus Thread [Re: Asura]
#26690632 - 05/23/20 03:08 PM (3 years, 8 months ago) |
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Quote:
Asura said: Those dense white masses are something I never want to see in a tray. It happens and it doesn't mean all is lost, but it hasn't ever been a good sign for me personally.
Once I lay a tray, I really don't like to keep it in recovery for longer than 5 days. So if it's ready it's ready and if not, I would put it in fruiting. Again, that's just my preference. But keep in mind, I am putting fully colonized sub in trays. If you are spawning directly to trays you could probably go a little longer.
yup yup
thats the difference right there celestial. when we are laying our trays our substrate is already 100% colonized cuz we had it colonize in bags. so when we lay to trays its just a matter of allowing it to recover 100% and then casing it. i know usually my trays are ready for casing by day 3. but like asura said if your spawning to trays like you would do with cubes its def gnna take longer. and u really dont know if your sub is actually 100% even in see through trays your really just making your best guess at if its fully colonized or not. thats one thing i really like about the bags cuz i can break it open and see for sure and if you have spots that aint they sure as shit will be once that shit is all mixed up and put to trays. kinda like shaking a jar
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Celestialexplorer1



Registered: 03/25/20
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Re: Official Copelandia/Panaeolus Thread [Re: Asura]
#26690638 - 05/23/20 03:10 PM (3 years, 8 months ago) |
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Yes I’m sure it isn’t cube. Thanks azura, I think I’m going to start doing manure and rye bags for panas just because of how fast they colonize and overlay and to kill that recovery time. I have a couple more quarts that should be done tomorrow I will have to spawn but after that no more. The overlay is too hard to control. It’s starts before the tray is even done colonizing
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Edited by Celestialexplorer1 (05/23/20 03:11 PM)
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jcm4620
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i think a lot of your issues celestial are due to the fact u didnt use agar on those grows and the mycelium type that you dont want is the type that you ended up with. i think you will find that when you select the growth you want from the start you will have far far better results and less of an issue with overlay.
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Celestialexplorer1



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Re: Official Copelandia/Panaeolus Thread [Re: jcm4620]
#26691138 - 05/23/20 08:00 PM (3 years, 8 months ago) |
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I agree and everything I have now coming is all 100% agar inoculated. I really never fully understood the importance until I actually worked with it and saw the difference and now I will never use a spore syringe again. The jars I have are all finishing three times the speed and all look so much healthier. I can’t wait to see what it can do in the fruiting chamber. I am almost done with the transfers from the spores you gave me. I’ll be posting pictures of that soon
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Celestialexplorer1



Registered: 03/25/20
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Here is my T6 I know I know it’s a little crowded in there I was short on plates at the time
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A.k.a
Stranger



Registered: 10/27/19
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Right on man lol saving space.
Yeah agar is definitely way more important with pans than cubes I’m finding out.
Myc selection makes a much bigger difference.
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Celestialexplorer1



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Re: Official Copelandia/Panaeolus Thread [Re: A.k.a]
#26691216 - 05/23/20 08:43 PM (3 years, 8 months ago) |
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Oh and also I believe the bacterial issue was due to gnats which have been seen in my GH lately. Bout to have to scrap everything in there and probably gonna go back to tubs for awhile.
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vinnie boombotz
Reggaejunkiejew


Registered: 04/29/19
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I've been collecting jumping spiders and putting them and my grow area they have a slight fly problem.
And this popped out of my snake plant today. But theres 2" of cubes spawn on top. I put some cyan Down a few months back but nothing ever happened

Edited by vinnie boombotz (05/24/20 02:17 AM)
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jcm4620
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yea celestial those look a lot better for sure take some transfers to new plates n get them things to grain asap. t6 is pretty far out from where i usually start my grain at. i like to get to grains as fast as possible. once i have the kind of growth im after and im sure its clean il put to grains. usually i end up on grain by t2 or t3. i just figure since im after clones at first anyway i want my potential gene pool as big as possible. eventually you start isolating away shit you may want without even knowing it. once you get your eye for pan myc on agar you will get there a lot faster. it just takes a few to really see what it is your looking for after looking at cubes so much
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Puduwoke
mushroom enthusiastic

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Re: Official Copelandia/Panaeolus Thread [Re: jcm4620]
#26691519 - 05/24/20 12:44 AM (3 years, 8 months ago) |
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Took out a print from our beloved Asura (Pan. Jam) 2 days ago and it's already germinated. My fastest time before with Pans was 4 days which I thought was crazy fast, this time 2 days! Love this species
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Adas
Lonely Dreamer



Registered: 12/22/16
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Quote:
vinnie boombotz said: I've been collecting jumping spiders and putting them and my grow area they have a slight fly problem.
And this popped out of my snake plant today. But theres 2" of cubes spawn on top. I put some cyan Down a few months back but nothing ever happened

Dat looks like Cinctulus.
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jcm4620
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Re: Official Copelandia/Panaeolus Thread [Re: Adas]
#26691866 - 05/24/20 06:08 AM (3 years, 8 months ago) |
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speakin of cincts i spawned my pan cyan bamas that i got from asura a cpl hrs ago and i found 2 tiny blue stones in the grain jars stuck on the bottom. now i have seen cyans stone on agar b4 but never in grains. especially after only 8 days from wedge drop. i just thought it was kinda cool is all. im sure im not the only 1 thats had this happen before but i cant belive they made stones that fast. i mean hows that even possible??? after seeing it i wished i didnt spawn it lol. i just wish my phone wasnt dead and in the other room charging so that i could have took a pic ☹️ im curious to if it may make some more in the bulk.
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Hans Wermhat
Human


Registered: 06/10/19
Posts: 167
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Re: Official Copelandia/Panaeolus Thread [Re: jcm4620]
#26692670 - 05/24/20 01:16 PM (3 years, 8 months ago) |
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huh that's interesting, never seen stones before on grains. Are they about the same size as the ones that grow on agar?
If anyone's been working on zifozonke's unknown pan, here are my notes so far. Grow route is agar->LC->grains->pasteurized bulk. It does fine on standard 2% MEA, nothing special or noteworthy regarding agar. They don't do very well on LC. Checking my notes, I've tried 2% malt extract LC, 1% malt extract, and 1% malt extract + 0.5% peptone. All three didn't look healthy, but worked fine to inoculate jars of rye. On grains they move at an okay rate, a bit slower than Pan cyans and the mycelium looks very wispy and weak, but they recover from shaking fine.
They colonized pasteurized 2:1:1 straw:coir:manure/humus(scott's brand) very very quickly and look very healthy. I was surprised, spawning to bulk really rejuvenated the culture and made it grow like a champ. Bulk was colonized in about 4 days, left to consolidate for another week, and then cased with standard pasteurized peat moss/verm and put in greenhouse. Temperatures are around 27C (80F) and it's been about a week but no pins yet.
Most trays were spawned 0.75 qt colonized rye to 3 qts of bulk (1:4 spawn:bulk ratio), and they all show some minor overlay. One tray was spawned 1.5 qt colonized rye to 3 qts bulk (1:2 ratio) and is showing a ton of overlay. So if you're working on this unknown species now or in the future definitely consider using a very low spawn ratio. The 1:4 colonized in just 4 days, so maybe even a 1:10 ratio would be sufficient and prevent the overlay. It would probably cut down on the time to pin too.
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jcm4620
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Re: Official Copelandia/Panaeolus Thread [Re: Hans Wermhat]
#26692694 - 05/24/20 01:25 PM (3 years, 8 months ago) |
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like i said they were very very small but they were definitley lil blue stones and they were smaller than what i have seen on my agar plates when i get them. like i said it was only 6 days from wedge drop to 100% colonization but they sat for 2 days after that til i had time to spawn. but still how the hell can they form that quick. if i had noticed before spawning i would have just let that jar go and see what i could get outta it by letting it sit for 3 or 4 months. ima check the bulk tho when it comes time to put to trays and if i find any il put em on agar and knock up another jar n just see what happens.
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vinnie boombotz
Reggaejunkiejew


Registered: 04/29/19
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Re: Official Copelandia/Panaeolus Thread [Re: Adas]
#26692801 - 05/24/20 02:31 PM (3 years, 8 months ago) |
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Wow good call.. I had a small jar of cints about a year ago there I thought one bacterial so I dumped it in Aus Flint so it's since on the bottom snow in in the middle and cube on top
I ate100 g of fresh cints then I had to go to work and deliver pizzas. I don't know if it was the 10 strip I did the few nights before, but they didn't really hit me that too much. Fresh. But yeah that's definitely a Pan sub or send telus whatever you wanna call it lol. I wish I had some Kane seeds is that even possible to get in this country?
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gizmo1



Registered: 06/15/11
Posts: 3,831
Loc: FREEDOM
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Any votes on which I should start with or which is less common? I have Pan Cambo Suphanburi Goliath, Pan Cyan Westmoreland Jamaica, and Pan Cyan KG of unknown origin.
 Im game to start with any of the 3 to hopefully get prints to spread.
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Celestialexplorer1



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Re: Official Copelandia/Panaeolus Thread [Re: gizmo1]
#26693291 - 05/24/20 05:57 PM (3 years, 8 months ago) |
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This is pan jam.. has anyone ever seen these off white masses like this.. it almost has the same form as pinning would but not quite so I’m pretty confident it is contam. Luckily it’s the last tray in my GH left before I tear it down and start on a new FC. I need something sealable that has no entrance for gnats. I’ve done all I can. I haven’t seen any since yesterday but I’m tired of the mental stress of it
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Edited by Celestialexplorer1 (05/24/20 05:59 PM)
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jcm4620
Stranger


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like i said before im certain its bacterial but i think u def need to get the wetness of your casing up extremely also the off white color can come from the mycelium kinda drying out and matting its self too
Edited by jcm4620 (05/24/20 06:16 PM)
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