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OfflinetaGyo
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Re: Official Copelandia/Panaeolus Thread [Re: d0urd3n]
    #22728378 - 01/02/16 02:36 PM (8 years, 29 days ago)

:fuckinawesome:


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InvisibleTeemo 6T3
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Re: Official Copelandia/Panaeolus Thread [Re: taGyo]
    #22731495 - 01/03/16 10:47 AM (8 years, 28 days ago)

Hmmm, some of my Pan transfers are bruising on agar, is there a reason for this? sign of a contam maybe?

I'm sure one of the plates is contaminated with bacteria, i see the colony but its far from the mycelium.... will be transferring a nice clean cotton part away from it.

So is there a reason behind Pan mycelium to bruise like this, or is it just normal?


Edited by Teemo 6T3 (01/03/16 10:48 AM)


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InvisibleMr.PhilCybin
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Re: Official Copelandia/Panaeolus Thread [Re: Teemo 6T3]
    #22731552 - 01/03/16 11:02 AM (8 years, 28 days ago)

How are you guys casing your pans?


I have a mini that I need to case today.


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I'm stupid, Falcon91Wolvrn03 is smart.
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I'm a loser, Falcon91Wolvrn03 is a winner.
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OfflineDeTwizzle
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Re: Official Copelandia/Panaeolus Thread [Re: Mr.PhilCybin]
    #22731564 - 01/03/16 11:05 AM (8 years, 28 days ago)

Jiffy mix, or some kind of non nutritious seed starter.


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OfflinetaGyo
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Re: Official Copelandia/Panaeolus Thread [Re: Teemo 6T3]
    #22731597 - 01/03/16 11:17 AM (8 years, 28 days ago)

Quote:

Teemo 6T3 said:
Hmmm, some of my Pan transfers are bruising on agar, is there a reason for this? sign of a contam maybe?

I'm sure one of the plates is contaminated with bacteria, i see the colony but its far from the mycelium.... will be transferring a nice clean cotton part away from it.

So is there a reason behind Pan mycelium to bruise like this, or is it just normal?



Bruising on agar? How long have they been on agar? If its turning blue on agar I think you have a minor bacteria problem underneath your culture. That sometimes gives specific parts an almost see-through wispiness when they come in contact with that.

Can you take a pic?


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InvisibleTeemo 6T3
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Re: Official Copelandia/Panaeolus Thread [Re: taGyo]
    #22731719 - 01/03/16 12:03 PM (8 years, 28 days ago)

Well i've been neglecting them since exams started, so I'd say around 1 week or so maybe..

Transfer plate 1, view up & down..


as you can see, there is bacteria growing on the side, but when you look below you see a yellowish colour where the mycelium is bruised, so i think you're right tagyo. (btw ignore the lumpy/shiny looking agar here, i moved the agar while it was semi solidifying so it got fucked up lol, so i transferred the wedge there any ways...cus i was like fuuck it)

This is plate 2 taken from below.


The yellow beneath, is also the spot where its bruising, so yes, you are right tagyo.

I'll be transferring the outermost clean mycelium when i have time. lol

Sorry for the kinda shitty pics, my iphone camera is getting fucked these days for some reason, can't take nice focused pictures as it use to do like my microscopic pics of Cyanobacteria and shit... I guess iphones just loathe mushroom mycelium for some reason lol  :crankey:


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InvisibleFantastic Mr. Fox
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Re: Official Copelandia/Panaeolus Thread [Re: Teemo 6T3]
    #22731736 - 01/03/16 12:10 PM (8 years, 28 days ago)

I know finals is whooping tht ass, but you better transfer soon.

2-3o'clock is where I'd go.


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OfflinePeacefrog
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Re: Official Copelandia/Panaeolus Thread [Re: Fantastic Mr. Fox]
    #22734406 - 01/04/16 02:05 AM (8 years, 27 days ago)

I love me some pans, nice thread.




I have recently been working with them invitro style using cakes with good results. Not great yields by any means, but easy and quick:


I even saw some grow true inviro:


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OfflineArgyreia
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Re: Official Copelandia/Panaeolus Thread [Re: Peacefrog]
    #22734498 - 01/04/16 03:26 AM (8 years, 27 days ago)

Quote:

Peacefrog said:
I love me some pans, nice thread.




I have recently been working with them invitro style using cakes with good results. Not great yields by any means, but easy and quick:


I even saw some grow true inviro:





What is your recipe for these cakes and how do you fruit them ?


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OfflinePeacefrog
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Re: Official Copelandia/Panaeolus Thread [Re: Argyreia]
    #22734570 - 01/04/16 04:35 AM (8 years, 27 days ago)

The recipe isn't anything new. I just use brf, manure, verm, coir and gypsum.

Inoculated with a slurry, either double end cased or dunked and rolled and fruited in small containers with holes for FAE. I place the cakes over a layer of field capacity casing and set and forget. Easy breezy.


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InvisibleTeemo 6T3
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Re: Official Copelandia/Panaeolus Thread [Re: Fantastic Mr. Fox]
    #22734638 - 01/04/16 05:32 AM (8 years, 27 days ago)

Niiiice peacefrog :rockon:

Quote:

Fantastic Mr. Fox said:
I know finals is whooping tht ass, but you better transfer soon.

2-3o'clock is where I'd go.



:lmafo:

Will do, hopefully tonight.


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OfflineArgyreia
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Re: Official Copelandia/Panaeolus Thread [Re: Teemo 6T3]
    #22734877 - 01/04/16 07:54 AM (8 years, 27 days ago)

Awesome work Peacefrog. Will try this asap :omgawesome:


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InvisibleFantastic Mr. Fox
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Re: Official Copelandia/Panaeolus Thread [Re: Peacefrog]
    #22734916 - 01/04/16 08:13 AM (8 years, 27 days ago)

Quote:

Peacefrog said:
I love me some pans, nice thread.




I have recently been working with them invitro style using cakes with good results. Not great yields by any means, but easy and quick:


I even saw some grow true inviro:





Nice, PF
&& welcome!

really like that "unmodified" tub  :smirk:


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Giving is all we have, for we're just grateful to be alive

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OfflinetaGyo
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Re: Official Copelandia/Panaeolus Thread [Re: Fantastic Mr. Fox]
    #22735587 - 01/04/16 11:44 AM (8 years, 27 days ago)

beautiful pics PF :rockon:


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InvisibleMr.PhilCybin
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Re: Official Copelandia/Panaeolus Thread [Re: taGyo]
    #22735615 - 01/04/16 11:51 AM (8 years, 27 days ago)

just cased some brazilian cyans last night.



it was kind of a "eh, fuck it I have this jar lets see if it works" kinda grow so I'm not expecting much.

I don't have any poo so I just spawned a quart or rye to cvg, it actually colonized, and then cased with a peat/verm/lime casing.

first time I've cased anything so lets see how it goes.
ill post pics if it does anything in a week or so


--------------------
I'm stupid, Falcon91Wolvrn03 is smart.
I'm ugly, Falcon91Wolvrn03 is beautiful.
I'm a loser, Falcon91Wolvrn03 is a winner.
Someday, I hope to be like Falcon91Wolvrn03 but secretly know I never will.


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InvisibleFantastic Mr. Fox
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Re: Official Copelandia/Panaeolus Thread [Re: Mr.PhilCybin]
    #22735715 - 01/04/16 12:29 PM (8 years, 27 days ago)

Brazilian?

Was tht a wild print?


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Giving is all we have, for we're just grateful to be alive

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InvisibleMr.PhilCybin
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Re: Official Copelandia/Panaeolus Thread [Re: Fantastic Mr. Fox]
    #22735723 - 01/04/16 12:31 PM (8 years, 27 days ago)

not sure.  I got it in a trade from another member along with a ton of others

pretty sure he grew it out and took the print though.
I have a maui i've been meaning to lay down aswell.


--------------------
I'm stupid, Falcon91Wolvrn03 is smart.
I'm ugly, Falcon91Wolvrn03 is beautiful.
I'm a loser, Falcon91Wolvrn03 is a winner.
Someday, I hope to be like Falcon91Wolvrn03 but secretly know I never will.


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OfflinePeacefrog
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Re: Official Copelandia/Panaeolus Thread [Re: Mr.PhilCybin]
    #22735810 - 01/04/16 12:58 PM (8 years, 27 days ago)

Thanks for the kind words all. 

I've been working with pans for some time now. They are by far my favorite active to work with thus far. I and another grower(who gifted me some spores) are working on a sub-strain that throws off leucistic and true albino pans. So lm looking forward to seeing them fruit.


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InvisibleFantastic Mr. Fox
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Re: Official Copelandia/Panaeolus Thread [Re: Mr.PhilCybin]
    #22735819 - 01/04/16 01:05 PM (8 years, 27 days ago)

Quote:

Mr.PhilCybin said:
not sure.  I got it in a trade from another member along with a ton of others

pretty sure he grew it out and took the print though.
I have a maui i've been meaning to lay down aswell.




Yess!!
Lay them dwn Mr. Phil


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Giving is all we have, for we're just grateful to be alive

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InvisibleFantastic Mr. Fox
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Re: Official Copelandia/Panaeolus Thread [Re: Peacefrog]
    #22735835 - 01/04/16 01:11 PM (8 years, 27 days ago)

Quote:

Peacefrog said:
Thanks for the kind words all. 

I've been working with pans for some time now. They are by far my favorite active to work with thus far. I and another grower(who gifted me some spores) are working on a sub-strain that throws off leucistic and true albino pans. So lm looking forward to seeing them fruit.




You && me both!

Leucistic huh?..
Interesting.
Is that of the cyanescens sp?
I know there has been record of the australian variety throwing out specimens with a red/rust colored spore deposit.


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