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Fantastic Mr. Fox
Grass Seed Connoisseur


Registered: 02/12/15
Posts: 3,225
Loc:
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Re: Official Copelandia/Panaeolus Thread [Re: Psilosopherr]
#22720976 - 12/31/15 12:31 PM (8 years, 1 month ago) |
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Here's some images for comparison.. (Pan cyan "Jamaica")
(before shake)
(after shake)
-------------------- Giving is all we have, for we're just grateful to be alive

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Psilosopherr
A psilly goose



Registered: 02/15/12
Posts: 12,278
Last seen: 1 month, 10 days
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such a sexy picture, what I would do for clean growth like that..
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Grey
⇜ ✯ ⇝



Registered: 11/06/14
Posts: 6,223
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Re: Official Copelandia/Panaeolus Thread [Re: Psilosopherr]
#22721508 - 12/31/15 03:02 PM (8 years, 1 month ago) |
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Update on my Pan. Cambo.
Pint master with bruised mycelium.

Single quart almost recovered from a shake.
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AMU Q&A If you don't have a plan of your own, you'll become a part of somebody else's.
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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 10 hours
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This is the culture I have in the fridge ^
M
Hmm. Fuck it looked like a clean jump off the agar, Ill give it a couple days and see what it looks like after it recovers all the way but yea that sucks
This is the culture I used I think does it look ok to you from what you can see? Ill get a better pic when my phone charges.
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 Amateur Mycologists United AMU Q&A
Edited by mushpunx (12/31/15 04:17 PM)
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Psilosoulful
Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Re: Official Copelandia/Panaeolus Thread [Re: mushpunx]
#22721835 - 12/31/15 04:54 PM (8 years, 30 days ago) |
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Looks okay, kinda whispy tho. I'd transfer it to another plate to be sure it's completely clean. What is your agar recipe? I've had great results using a classic PDA mix.
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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 10 hours
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Re: Official Copelandia/Panaeolus Thread [Re: Psilosoulful]
#22721860 - 12/31/15 05:01 PM (8 years, 30 days ago) |
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Quote:
Psilosoulful said: Looks okay, kinda whispy tho. I'd transfer it to another plate to be sure it's completely clean. What is your agar recipe? I've had great results using a classic PDA mix.
I usually use MEA but yea I switch back and forth between that and PDA. Classic recipe too, usually lower nutes though that's probably why its wispy?
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 Amateur Mycologists United AMU Q&A
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Psilosoulful
Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Re: Official Copelandia/Panaeolus Thread [Re: mushpunx]
#22721891 - 12/31/15 05:13 PM (8 years, 30 days ago) |
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Quote:
mushpunx said:
Quote:
Psilosoulful said: Looks okay, kinda whispy tho. I'd transfer it to another plate to be sure it's completely clean. What is your agar recipe? I've had great results using a classic PDA mix.
I usually use MEA but yea I switch back and forth between that and PDA. Classic recipe too, usually lower nutes though that's probably why its wispy?
I used the recipe at the end of Pasty's prep video. The mycelium was nice and rather thick tomentose growth.
Could be that a contam got in early and that's been hindering good growth in your plate.
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Fantastic Mr. Fox
Grass Seed Connoisseur


Registered: 02/12/15
Posts: 3,225
Loc:
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Re: Official Copelandia/Panaeolus Thread [Re: mushpunx]
#22724093 - 01/01/16 10:08 AM (8 years, 30 days ago) |
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Quote:
mushpunx said:
Quote:
Psilosoulful said: Looks okay, kinda whispy tho. I'd transfer it to another plate to be sure it's completely clean. What is your agar recipe? I've had great results using a classic PDA mix.
I usually use MEA but yea I switch back and forth between that and PDA. Classic recipe too, usually lower nutes though that's probably why its wispy?
Less nutes..? What's your recipe?
I ask because, less nutrients = faster colonization, which is good&bad. You want any microorganism on the plate to have enough nutrients to express themselves appropriately/early on. with lower nutrients, the mold or bacteria doesn't have much access to uncaptured nutrients to show themselves & sporulate, not to mention since the mycelium is also running quicker, due to the low nutrient makeup, you might be tricked into thinking it is clean, when really there is mold/bacteria hitching onto your culture.
-------------------- Giving is all we have, for we're just grateful to be alive

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Fantastic Mr. Fox
Grass Seed Connoisseur


Registered: 02/12/15
Posts: 3,225
Loc:
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Re: Official Copelandia/Panaeolus Thread [Re: Grey]
#22724104 - 01/01/16 10:11 AM (8 years, 30 days ago) |
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Quote:
Grey said: Update on my Pan. Cambo.
Pint master with bruised mycelium.

 I remember my cambo jars would always bruise as well. First occurrence almost gave me a heart attack lol
-------------------- Giving is all we have, for we're just grateful to be alive

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Grey
⇜ ✯ ⇝



Registered: 11/06/14
Posts: 6,223
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Yeah dude, I thought it was green. It turned on my daylight and it's blue. Phew...I hope.
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AMU Q&A If you don't have a plan of your own, you'll become a part of somebody else's.
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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 10 hours
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Quote:
Fantastic Mr. Fox said:
Quote:
mushpunx said:
Quote:
Psilosoulful said: Looks okay, kinda whispy tho. I'd transfer it to another plate to be sure it's completely clean. What is your agar recipe? I've had great results using a classic PDA mix.
I usually use MEA but yea I switch back and forth between that and PDA. Classic recipe too, usually lower nutes though that's probably why its wispy?
Less nutes..? What's your recipe?
I ask because, less nutrients = faster colonization, which is good&bad. You want any microorganism on the plate to have enough nutrients to express themselves appropriately/early on. with lower nutrients, the mold or bacteria doesn't have much access to uncaptured nutrients to show themselves & sporulate, not to mention since the mycelium is also running quicker, due to the low nutrient makeup, you might be tricked into thinking it is clean, when really there is mold/bacteria hitching onto your culture.
Thats an interesting point Fox Ill remember to take that into account in the future certainly! I dont know if my regular recipe is low enough to make that sort of difference though?
Id say a classic recipe is 10g agar 10 ME to 500ml water? I usually go maybe 10 g 8g ME to 500ml. So its a little lower nutrition
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 Amateur Mycologists United AMU Q&A
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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Re: Official Copelandia/Panaeolus Thread [Re: mushpunx]
#22724251 - 01/01/16 11:22 AM (8 years, 30 days ago) |
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I searched Google looking for some of me old deleted pix. I found this one of pan cyan from wide spores producing pseudo sclerotia. I grew this culture out dozens of times and only got fruits once. Every other time it Wes these ugly stones.
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d0urd3n
Just call me "D"


Registered: 09/15/10
Posts: 5,237
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Re: Official Copelandia/Panaeolus Thread [Re: MudaFuka]
#22724255 - 01/01/16 11:24 AM (8 years, 30 days ago) |
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Interesting. Did you ever eat any?
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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Re: Official Copelandia/Panaeolus Thread [Re: d0urd3n]
#22724258 - 01/01/16 11:27 AM (8 years, 30 days ago) |
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No I never did. I wish I had. Pasty grew with the same spores and had the same result. He didn't eat them either.
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d0urd3n
Just call me "D"


Registered: 09/15/10
Posts: 5,237
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Re: Official Copelandia/Panaeolus Thread [Re: MudaFuka]
#22724272 - 01/01/16 11:32 AM (8 years, 30 days ago) |
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Hmm. Might be worth messing with if that print is still around.
I've spammed these in like three threads now, but screw it. Belongs here too.
Panaeolus cyanescens Hawaii

Panaeolus bisporus Colville

I think it's worth mentioning that on my first one or two goes with the Pan bisporus (all separate MS attempts from the same print) the myc on grain looked much wispier than this third attempt. It looked the same on agar as the first two attempts though. Grain prep was similar, albeit perhaps less wet this time. Maybe it likes oats more than WBS or rye, I doubt that should matter though...
Other grain jar examples:

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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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Re: Official Copelandia/Panaeolus Thread [Re: d0urd3n]
#22724301 - 01/01/16 11:44 AM (8 years, 30 days ago) |
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I think I kept a little piece of that print tucked away somwhere. I don't have time to mess with it right now though.
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dusttodust

Registered: 11/26/12
Posts: 491
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Re: Official Copelandia/Panaeolus Thread [Re: MudaFuka]
#22724644 - 01/01/16 02:07 PM (8 years, 30 days ago) |
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I think its time to stop calling stones from panaeolus pseudosclerotia. These are normal sclerotia. This guy made a bunch of them suprisingly http://www.cyber-shrooms.com/growing/project-rare-truffle-species/
Seeing how fragile some panaeoluses are it makes sense they make truffles to survive better in the nature. Here are goliath truffles lol
Edited by dusttodust (01/02/16 11:49 AM)
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Psilosopherr
A psilly goose



Registered: 02/15/12
Posts: 12,278
Last seen: 1 month, 10 days
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Quote:
Fantastic Mr. Fox said:
Quote:
mushpunx said:
Quote:
Psilosoulful said: Looks okay, kinda whispy tho. I'd transfer it to another plate to be sure it's completely clean. What is your agar recipe? I've had great results using a classic PDA mix.
I usually use MEA but yea I switch back and forth between that and PDA. Classic recipe too, usually lower nutes though that's probably why its wispy?
Less nutes..? What's your recipe?
I ask because, less nutrients = faster colonization, which is good&bad. You want any microorganism on the plate to have enough nutrients to express themselves appropriately/early on. with lower nutrients, the mold or bacteria doesn't have much access to uncaptured nutrients to show themselves & sporulate, not to mention since the mycelium is also running quicker, due to the low nutrient makeup, you might be tricked into thinking it is clean, when really there is mold/bacteria hitching onto your culture.
very interesting, I can imagine an application or two
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Nobler Hino
a dojo and a forge?!



Registered: 08/29/15
Posts: 1,780
Loc: Deep Ellum
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Re: Official Copelandia/Panaeolus Thread [Re: Psilosopherr]
#22726102 - 01/01/16 11:40 PM (8 years, 29 days ago) |
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Yeah that is a awesome agar tip, ill remember that next run.
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   "The sacred mushroom takes me by the hand and brings me to the world where everything is known. It is they, the sacred mushrooms, that speak in a way I can understand. I ask them and they answer me.” – Maria Sabina
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d0urd3n
Just call me "D"


Registered: 09/15/10
Posts: 5,237
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Re: Official Copelandia/Panaeolus Thread [Re: Nobler Hino]
#22727708 - 01/02/16 11:51 AM (8 years, 29 days ago) |
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These are all Panaeolus cintulus. Found on the same pile of hpoo, at the same time. Thought it interesting to see the difference between how they look based on where they grew. Super fat on straight dung. Towards the grass and in more shade, much smaller caps, thinner stipes, never seen bluing on any of the small ones, but the fat guys have bruised strongly for me before. Not always though.




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