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InvisibleTeemo 6T3
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Re: Official Copelandia/Panaeolus Thread [Re: Nobler Hino]
    #22711902 - 12/29/15 04:21 AM (8 years, 1 month ago)

So guys... i transferred a wedge, and some how i got mycelium looking fuzz  on the sides of agar, i think water transferred them there OR they could be  contaminants.

Not too sure because they're taking their sweet-ass time as if they're Pan mycelium.  What do you guys think?


Ignore those speckles they're just parts of potato flakes that didn't dissolve...

And it seems that its still showing some wispy-ness on the outer edge, some of it seems like its eating through the agar again, could it be that I'm preparing my agar to be on the soft side?

Because i just eye ball my ingredients lol.


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InvisibleNobler Hino
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Re: Official Copelandia/Panaeolus Thread [Re: Teemo 6T3]
    #22712036 - 12/29/15 06:35 AM (8 years, 1 month ago)

Quote:

Teemo 6T3 said:
So guys... i transferred a wedge, and some how i got mycelium looking fuzz  on the sides of agar, i think water transferred them there OR they could be  contaminants.

Not too sure because they're taking their sweet-ass time as if they're Pan mycelium.  What do you guys think?


Ignore those speckles they're just parts of potato flakes that didn't dissolve...

And it seems that its still showing some wispy-ness on the outer edge, some of it seems like its eating through the agar again, could it be that I'm preparing my agar to be on the soft side?

Because i just eye ball my ingredients lol.




Those circles are contams, transfer far away from them. What is your agar recipe?


--------------------

"The sacred mushroom takes me by the hand and brings me to the world where everything is known. It is they, the sacred mushrooms, that speak in a way I can understand. I ask them and they answer me.”
– Maria Sabina


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OfflinePsilosopherr
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Re: Official Copelandia/Panaeolus Thread [Re: Nobler Hino]
    #22712495 - 12/29/15 10:23 AM (8 years, 1 month ago)

Recently tried straining by agar after dissolving it in water and have been very happy with how much more clear it is when this is done.


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InvisibleMad Season
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Re: Official Copelandia/Panaeolus Thread [Re: Psilosopherr]
    #22716292 - 12/30/15 09:29 AM (8 years, 1 month ago)



Fox's pan cambo.. 48 hours post shake


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InvisibleNobler Hino
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Re: Official Copelandia/Panaeolus Thread [Re: Mad Season]
    #22716459 - 12/30/15 10:30 AM (8 years, 1 month ago)

Hey guys, was just wondering if these caps are going to open up anymore. They are about a week old



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"The sacred mushroom takes me by the hand and brings me to the world where everything is known. It is they, the sacred mushrooms, that speak in a way I can understand. I ask them and they answer me.”
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OfflinetaGyo
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Re: Official Copelandia/Panaeolus Thread [Re: Nobler Hino]
    #22716524 - 12/30/15 10:56 AM (8 years, 1 month ago)

They look so cute :lol:


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Invisiblethe_r3dz
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Re: Official Copelandia/Panaeolus Thread [Re: taGyo]
    #22718640 - 12/30/15 09:07 PM (8 years, 1 month ago)



How many holes do you think this tub needs? It's huge, 100L with four 6qt minitubs.

Should I put holes into the minitubs? They are 4" tall but I'm only going to do about 2" of substrate in each one. I was thinking of lining up the holes of the minitubs and the outer tubs, one on each long side of the mini and stuffing the outer tub with poly


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Re: Official Copelandia/Panaeolus Thread [Re: the_r3dz]
    #22720183 - 12/31/15 09:33 AM (8 years, 1 month ago)




Ok so this is Pan Cyan spawn shaken a week or so ago

Its uniformly colonized but it still needs more time right? Should look tottally white like a cube jar?

  This is my first Pan spawn :shrug:

Thx!


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InvisibleNobler Hino
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Re: Official Copelandia/Panaeolus Thread [Re: mushpunx]
    #22720194 - 12/31/15 09:37 AM (8 years, 1 month ago)

That actually looks good man, you could spawn that. Pan myc isn't nearly as thick as cubes.


--------------------

"The sacred mushroom takes me by the hand and brings me to the world where everything is known. It is they, the sacred mushrooms, that speak in a way I can understand. I ask them and they answer me.”
– Maria Sabina


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InvisibleTeemo 6T3
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Re: Official Copelandia/Panaeolus Thread [Re: Nobler Hino]
    #22720203 - 12/31/15 09:40 AM (8 years, 1 month ago)

:whathesaid:

My fully colonized spawn is looking like that also..


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InvisibleTeemo 6T3
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Re: Official Copelandia/Panaeolus Thread [Re: Nobler Hino]
    #22720229 - 12/31/15 09:45 AM (8 years, 1 month ago)

Quote:

Nobler Hino said:
Quote:

Teemo 6T3 said:
So guys... i transferred a wedge, and some how i got mycelium looking fuzz  on the sides of agar, i think water transferred them there OR they could be  contaminants.

Not too sure because they're taking their sweet-ass time as if they're Pan mycelium.  What do you guys think?


Ignore those speckles they're just parts of potato flakes that didn't dissolve...

And it seems that its still showing some wispy-ness on the outer edge, some of it seems like its eating through the agar again, could it be that I'm preparing my agar to be on the soft side?

Because i just eye ball my ingredients lol.




Those circles are contams, transfer far away from them. What is your agar recipe?



You sure? Becuase i checked on em today, and it seems they colonizing like they're normal Pan mycelium, kinda weird, i think its due to the water on the agar before i put the transfers in, they might of transferred pieces of mycelium there lol, we'll have to see

They agar recipe is just normal agar from the asian shop, potato flakes, and a drop of karo.... Eye balled all the measurements lol


--------------------
Shrooming Is Of The Essence


Rest In Peace Dankington


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Offlinemushpunx
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Re: Official Copelandia/Panaeolus Thread [Re: Nobler Hino]
    #22720262 - 12/31/15 09:51 AM (8 years, 1 month ago)

Quote:

Nobler Hino said:
That actually looks good man, you could spawn that. Pan myc isn't nearly as thick as cubes.





Really  ? Right on that's awesome



I gunna have to spawn to coir most likely, maybe Ill go coir-straw sub. Cant get manure around here this time of year


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InvisibleNobler Hino
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Re: Official Copelandia/Panaeolus Thread [Re: Teemo 6T3]
    #22720277 - 12/31/15 09:54 AM (8 years, 1 month ago)

I never trust circles in dishes if you've already transferred a sector. For agar I use 2 teaspoons of agar and half a teaspoon of malt extract mixed with 250 ml of water. You could even cut down the malt even more, I heard the mycelium will spread faster looking for nutrients.


--------------------

"The sacred mushroom takes me by the hand and brings me to the world where everything is known. It is they, the sacred mushrooms, that speak in a way I can understand. I ask them and they answer me.”
– Maria Sabina


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InvisibleNobler Hino
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Re: Official Copelandia/Panaeolus Thread [Re: mushpunx]
    #22720288 - 12/31/15 09:56 AM (8 years, 1 month ago)

Quote:

mushpunx said:
Quote:

Nobler Hino said:
That actually looks good man, you could spawn that. Pan myc isn't nearly as thick as cubes.





Really  ? Right on that's awesome



I gunna have to spawn to coir most likely, maybe Ill go coir-straw sub. Cant get manure around here this time of year




Right now I'm trying pan cyans on coir for the first time too, I ran out of manure. It shouldn't be a issue, gunna put them in fruiting conditions tomorrow :mushroom2:


--------------------

"The sacred mushroom takes me by the hand and brings me to the world where everything is known. It is they, the sacred mushrooms, that speak in a way I can understand. I ask them and they answer me.”
– Maria Sabina


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Re: Official Copelandia/Panaeolus Thread [Re: Nobler Hino]
    #22720343 - 12/31/15 10:13 AM (8 years, 1 month ago)

A typical agar recipe is 10 grams agar to 500 ml water, 10 G malt extract

I usually go between 5 and 10 grams malt extract.  Lower nutes will give more ropey rhizomorphic growth while nutrition agar give fluffy tometose growth


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OfflinePsilosopherr
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Re: Official Copelandia/Panaeolus Thread [Re: mushpunx]
    #22720371 - 12/31/15 10:20 AM (8 years, 1 month ago)

:takingnotes: Still trying to decide whether to switch away from pre-mixed nutrient agar. seems like mixing it yourself is the way to go


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Re: Official Copelandia/Panaeolus Thread [Re: mushpunx]
    #22720517 - 12/31/15 10:46 AM (8 years, 1 month ago)

Quote:

mushpunx said:



Ok so this is Pan Cyan spawn shaken a week or so ago

Its uniformly colonized but it still needs more time right? Should look tottally white like a cube jar?

  This is my first Pan spawn :shrug:

Thx!




Do not spawn tht jar.


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Offlinemushpunx
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Re: Official Copelandia/Panaeolus Thread [Re: Fantastic Mr. Fox]
    #22720568 - 12/31/15 10:54 AM (8 years, 1 month ago)

Quote:

Fantastic Mr. Fox said:
Quote:

mushpunx said:



Ok so this is Pan Cyan spawn shaken a week or so ago

Its uniformly colonized but it still needs more time right? Should look tottally white like a cube jar?

  This is my first Pan spawn :shrug:

Thx!




Do not spawn tht jar.






So it needs more time or is there something wrong ?


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InvisibleFantastic Mr. Fox
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Re: Official Copelandia/Panaeolus Thread [Re: mushpunx]
    #22720850 - 12/31/15 11:54 AM (8 years, 1 month ago)

That isn't clean pan spawn.


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OfflinePsilosopherr
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Re: Official Copelandia/Panaeolus Thread [Re: Fantastic Mr. Fox]
    #22720939 - 12/31/15 12:19 PM (8 years, 1 month ago)

:omgz:


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