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InvisibleTeemo 6T3
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Re: Official Copelandia/Panaeolus Thread [Re: Psilosoulful]
    #22675508 - 12/19/15 11:47 AM (8 years, 1 month ago)

I use pasty's recipe also, but i just like to eye ball the amount of agar and flakes im putting in depending how much plates I'm doing lol.

And i have no problem with getting Pan mycelium to get going, these actually germinated in like 5 days or something, i guess they're nice and fresh spores.

But it's really weird they had this growth pattern, those parts which fox thought were bacteria (which actually seemed to be so because of the crappy picture), were to my guess, just mycelium which colonized its way through the agar and had weak/thin mat of mycelium on the surface, its very intriguing. ( that's why i think some parts of agar were losing their reddish colour and turning whitish/yellowish)

But we will tell in a day or so, I'll take pictures of the left over agar that was thought to be contaminated (they were transferred to grain), leaving aside the transferred 9 and 12 o'clock that were transferred to another agar dish to be further cleaned.


Edited by Teemo 6T3 (12/19/15 11:51 AM)


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OfflinePsilosoulful

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Re: Official Copelandia/Panaeolus Thread [Re: Teemo 6T3]
    #22675585 - 12/19/15 12:07 PM (8 years, 1 month ago)

That's interesting.
The agar will change to a whitish/yellowish color as the mycelium eats it's way all the way through the agar.
That usually happens when the surface is completely covered tho.
If it happens before that, then there could be a high level of bacteria present as Fox pointed out before.


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Re: Official Copelandia/Panaeolus Thread [Re: Psilosoulful]
    #22675629 - 12/19/15 12:18 PM (8 years, 1 month ago)

Quote:

Psilosoulful said:
Really? I thought the poly wouldn't be a huge factor in colonization times. I was checking out Azur's thread on stuffing vs covering holes at spawning, I figured it would work the same for pans?

EDIT: Decided to go ahead and tape up those bottom holes anyway. The uneven lid should provide enough GE.

Day 5





works well with cubes. i haven't tried the colonizatoion with poly for pans though. always did a tray with either foil over the top or saran wrap during colonization.
i have to find this pan cyan plate i have hiding for the new year.


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OfflinePsilosoulful

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Re: Official Copelandia/Panaeolus Thread [Re: eatyualive]
    #22675649 - 12/19/15 12:27 PM (8 years, 1 month ago)

Quote:

eatyualive said:
Quote:

Psilosoulful said:
Really? I thought the poly wouldn't be a huge factor in colonization times. I was checking out Azur's thread on stuffing vs covering holes at spawning, I figured it would work the same for pans?

EDIT: Decided to go ahead and tape up those bottom holes anyway. The uneven lid should provide enough GE.

Day 5





works well with cubes. i haven't tried the colonizatoion with poly for pans though. always did a tray with either foil over the top or saran wrap during colonization.
i have to find this pan cyan plate i have hiding for the new year.



Cool, hopefully this tub will be ready to case in the next 5 days or so. Then it's fruiting time! :woot:


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Invisibledusttodust

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Re: Official Copelandia/Panaeolus Thread [Re: Teemo 6T3]
    #22675848 - 12/19/15 01:32 PM (8 years, 1 month ago)

Its not that unusual for culture to have different types of mycelium. Sclerotia for example has fast/white/dense and slower/thinner/more colorful mycelium. But it is usualy only 1 type when you have isolate. P. cint for example also makes pseudosclerotia and p. cint plate looks weird too so I wouldnt be so narrow looking, if you belive your spores germinated than you probably have the right culture. If you want to be sure you have pan and not mold, make few transfers and check if the leftover agar bruises.
If you want to be sure you dont have bacteria (which can be recognized by metabolites and weird bruising, tho bruising sometimes occurs naturally) you can drop wedge in hot agar and transfer the first mycelium you see growing.
Simple as that

Btw i think your pics look fine Teemo
The mushpunx guy in 48 page also have what looks like a legit culture IMO. He should check if it bruises

edit: http://www.cyber-shrooms.com/growing/truffles-panaeolus-subbalteatus-blue-pearls/


Edited by dusttodust (12/19/15 02:11 PM)


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InvisibleTeemo 6T3
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Re: Official Copelandia/Panaeolus Thread [Re: dusttodust]
    #22675863 - 12/19/15 01:37 PM (8 years, 1 month ago)

:takingnotes:


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InvisibleMad Season
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Re: Official Copelandia/Panaeolus Thread [Re: dusttodust] * 1
    #22675947 - 12/19/15 02:15 PM (8 years, 1 month ago)

Quote:

dusttodust said:
Its not that unusual for culture to have different types of mycelium. Sclerotia for example has fast/white/dense and slower/thinner/more colorful mycelium. But it is usualy only 1 type when you have isolate. P. cint for example also makes pseudosclerotia and p. cint plate looks weird too so I wouldnt be so narrow looking, if you belive your spores germinated than you probably have the right culture. If you want to be sure you have pan and not mold, make few transfers and check if the leftover agar bruises.
If you want to be sure you dont have bacteria (which can be recognized by metabolites and weird bruising, tho bruising sometimes occurs naturally) you can drop wedge in hot agar and transfer the first mycelium you see growing.
Simple as that

Btw i think your pics look fine Teemo
The mushpunx guy in 48 page also have what looks like a legit culture IMO. He should check if it bruises



http://www.shroomery.org/forums/showflat.php/Number/22020260

Although I'm not saying every funky white growth means it's a contamination... However, all those examples are contams. If you want to be 100% sure it's clean, and you don't have to roll the dice every time you grow, aim for organisation.

As stated in that thread, even pans can be organised.

This is a 100% clean pan culture. Using this I wouldn't be saying I'm making a risk come inocultion/spawn time. And it only took me 2 transfers to get it like this.


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InvisibleTeemo 6T3
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Re: Official Copelandia/Panaeolus Thread [Re: Mad Season]
    #22676031 - 12/19/15 02:39 PM (8 years, 1 month ago)

Wow, from now on i think I'll be more cautious lol, and i guess I'll be working with agar before shooting up jars..

You have enlightened me Mad Season, thank you :takingnotes: :bow2:


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Edited by Teemo 6T3 (12/19/15 02:44 PM)


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OfflinePsilosoulful

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Re: Official Copelandia/Panaeolus Thread [Re: Teemo 6T3]
    #22676490 - 12/19/15 05:13 PM (8 years, 1 month ago)

Quote:

Teemo 6T3 said:
Wow, from now on i think I'll be more cautious lol, and i guess I'll be working with agar before shooting up jars..



:awesomenod::thumbup:


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InvisibleTeemo 6T3
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Re: Official Copelandia/Panaeolus Thread [Re: Psilosoulful]
    #22678458 - 12/20/15 06:50 AM (8 years, 1 month ago)

Here is the jar that i transferred the remaining agar to.. Kinda crappy pictures so sorry lol.

Seems to be doing quite well



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Edited by Teemo 6T3 (12/20/15 06:55 AM)


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OfflinePsilosoulful

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Re: Official Copelandia/Panaeolus Thread [Re: Teemo 6T3]
    #22678822 - 12/20/15 10:01 AM (8 years, 1 month ago)

That's pretty decent growth, your grains look a bit dry tho.


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OfflinetaGyo
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Re: Official Copelandia/Panaeolus Thread [Re: Psilosoulful]
    #22678890 - 12/20/15 10:22 AM (8 years, 1 month ago)

You play league Teemo?


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InvisibleTeemo 6T3
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Re: Official Copelandia/Panaeolus Thread [Re: taGyo]
    #22679062 - 12/20/15 11:00 AM (8 years, 1 month ago)

Ye hahaha, i started playing it for one reason..... FUCKING TEEMO GOD!

Growing them shrooms and shit! such a toxic champ, just like me :laugh2:

You play also? :omgz:


Quote:

Psilosoulful said:
That's pretty decent growth, your grains look a bit dry tho.



Eh, its just the outside, they're pretty plump on the inside :pimp:


Edited by Teemo 6T3 (12/20/15 11:02 AM)


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OfflinetaGyo
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Re: Official Copelandia/Panaeolus Thread [Re: Teemo 6T3]
    #22679174 - 12/20/15 11:28 AM (8 years, 1 month ago)

Yeah me and a few other members :lol:


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OfflinePsilosoulful

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Re: Official Copelandia/Panaeolus Thread [Re: taGyo]
    #22679296 - 12/20/15 12:01 PM (8 years, 1 month ago)

Day 7:

The tub is starting to fluff up nicely. When should I consider casing and fruiting this? As you can see, the sides are barely colonized. I might wait for the whole thing to finish up.



Edited by Psilosoulful (12/20/15 12:02 PM)


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OfflinetaGyo
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Re: Official Copelandia/Panaeolus Thread [Re: Psilosoulful]
    #22679299 - 12/20/15 12:02 PM (8 years, 1 month ago)

Nice looking tub psilo :super:


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OfflinePsilosoulful

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Re: Official Copelandia/Panaeolus Thread [Re: taGyo]
    #22679313 - 12/20/15 12:06 PM (8 years, 1 month ago)

Thanks man, gotta love that hpoo!! :awesomenod:


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InvisibleTeemo 6T3
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Re: Official Copelandia/Panaeolus Thread [Re: Psilosoulful]
    #22679342 - 12/20/15 12:13 PM (8 years, 1 month ago)

Quote:

taGyo said:
Yeah me and a few other members :lol:



Nice! but i guess you're playing in american servers :sad: , would of been fun if we could play some day.
Quote:

Psilosoulful said:
Thanks man, gotta love that hpoo!! :awesomenod:



Yessir :rockon:

Better post pics when they pin  :tokeeporder:


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OfflinePsilosoulful

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Re: Official Copelandia/Panaeolus Thread [Re: Teemo 6T3]
    #22679416 - 12/20/15 12:25 PM (8 years, 1 month ago)

Quote:

Teemo 6T3 said:
Quote:

Psilosoulful said:
Thanks man, gotta love that hpoo!! :awesomenod:



Yessir :rockon:

Better post pics when they pin  :tokeeporder:



Don't worry, I will. I have to let it consolidate a bit tho. I want some healthy pins :yesnod:


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Re: Official Copelandia/Panaeolus Thread [Re: Psilosoulful]
    #22680282 - 12/20/15 04:13 PM (8 years, 1 month ago)

Alright, I finally have something to contribute to this thread. I germinated some Pan. Cambo spores (Thank you Mr. Fox :hatsoff:)  on 33% grain water agar. They germinated after a few days. Here's a pint master I inoculated on the 5th via wedge.



I have a quart going as well that I'm going to spawn in about a week.


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