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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Yes, but if you see the speckled shit on agar when you swipe, it's considered a mutant, and isn't worth your time. You can actually see some speckled areas on the outsides of some swipe areas, and even 2 strokes are all speckled mutants. You want to get colonies that are thick, and connected if you want the best yeast for ale.
Lol but let's get back on topic
Edited by Mad Season (12/18/15 11:21 AM)
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dusttodust

Registered: 11/26/12
Posts: 491
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Re: Official Copelandia/Panaeolus Thread [Re: Mad Season]
#22671924 - 12/18/15 01:15 PM (8 years, 1 month ago) |
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My cambo mycelium looked like what you guys I mentioned have Mycelium I had grew like teemos 6t3 red plates. Some parts of mycelium on plate were thick and some were thin and fragile looking like in r3dz plate. Thats why I think Fox suspects bacteria in dat red plate but it is normal for this myc to looks weird. It will never look symetric or nice, even with isolate. My isolate still "sectors" on thicker and fragile myc. But it is a bit of surprise that it is so different from p.cyan that people even think its contamned. I never workd with p.cyan tho
Btw, I never had much luck with grains. I recommend sterile sub
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Teemo 6T3
႟тнe мedιcιne мan ☼



Registered: 07/21/14
Posts: 1,570
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Re: Official Copelandia/Panaeolus Thread [Re: dusttodust]
#22672109 - 12/18/15 01:52 PM (8 years, 1 month ago) |
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Well I'm testing that out now, I've transferred the two sectors Mr fox told me to transfer and clean out.... And also transferred the remaining mycelium on the agar to a grain jar to see what contams are present and if it will colonize or just stall.
-------------------- Shrooming Is Of The Essence   Rest In Peace Dankington
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Re: Official Copelandia/Panaeolus Thread [Re: Teemo 6T3]
#22672624 - 12/18/15 04:01 PM (8 years, 1 month ago) |
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Those pesky contams!  Make sure to do all procedures in your SAB And always look to improve your sterile techniques
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SalviaGod
Bad Person, Good Guy



Registered: 05/07/12
Posts: 747
Last seen: 6 days, 45 minutes
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Re: Official Copelandia/Panaeolus Thread [Re: Psilosoulful]
#22672652 - 12/18/15 04:08 PM (8 years, 1 month ago) |
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Is there any benifit to consolidating your bulk substarte with pans before applying the casing layer and fruiting?
Or should you just case/fruit at 100%?
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Re: Official Copelandia/Panaeolus Thread [Re: SalviaGod]
#22672719 - 12/18/15 04:22 PM (8 years, 1 month ago) |
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It's personal preference really. The sub will pin up when it is fully colonized inside anyway, So giving it the extra couple of days with the holes covered isn't gonna be a big deal. If you use a clear liner, then you can see what percent of the bulk is colonized, and know exactly when to fruit.
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Teemo 6T3
႟тнe мedιcιne мan ☼



Registered: 07/21/14
Posts: 1,570
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Re: Official Copelandia/Panaeolus Thread [Re: Psilosoulful]
#22672792 - 12/18/15 04:43 PM (8 years, 1 month ago) |
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Quote:
Psilosoulful said: Those pesky contams!  Make sure to do all procedures in your SAB And always look to improve your sterile techniques
Always do in my medium sized glass aquarium gotta get a bigger one though.
Btw thanks for the link man, really nice write up by Mad season  I salute him
-------------------- Shrooming Is Of The Essence   Rest In Peace Dankington
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Re: Official Copelandia/Panaeolus Thread [Re: Teemo 6T3]
#22673045 - 12/18/15 05:57 PM (8 years, 1 month ago) |
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Quote:
Teemo 6T3 said:
Quote:
Psilosoulful said: Those pesky contams!  Make sure to do all procedures in your SAB And always look to improve your sterile techniques
Always do in my medium sized glass aquarium gotta get a bigger one though.
Btw thanks for the link man, really nice write up by Mad season  I salute him 
Yeah, thanks Mad! 
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Fantastic Mr. Fox
Grass Seed Connoisseur


Registered: 02/12/15
Posts: 3,225
Loc:
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Re: Official Copelandia/Panaeolus Thread [Re: dusttodust]
#22673433 - 12/18/15 07:33 PM (8 years, 1 month ago) |
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Quote:
dusttodust said: My cambo mycelium looked like what you guys I mentioned have Mycelium I had grew like teemos 6t3 red plates. Some parts of mycelium on plate were thick and some were thin and fragile looking like in r3dz plate. Thats why I think Fox suspects bacteria in dat red plate but it is normal for this myc to looks weird. It will never look symetric or nice, even with isolate. My isolate still "sectors" on thicker and fragile myc. But it is a bit of surprise that it is so different from p.cyan that people even think its contamned. I never workd with p.cyan tho
Btw, I never had much luck with grains. I recommend sterile sub
Oh rlyy, because all of my current pan cam,cyan,& trop plates are thick, organized and uniform.
Dude I'm not trying to be a dick here, but can you please stop passing around faulty advice. If not, please see yourself out. I can't condone that type of behavior/trolling..
The members in this thread are a hard working bunch, they don't need advice that's going to send them astray..
-------------------- Giving is all we have, for we're just grateful to be alive

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Fantastic Mr. Fox
Grass Seed Connoisseur


Registered: 02/12/15
Posts: 3,225
Loc:
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Quote:
Psilosoulful said: It's personal preference really. The sub will pin up when it is fully colonized inside anyway, So giving it the extra couple of days with the holes covered isn't gonna be a big deal..
Not true, Ive had a few pan subs that I cased/fruited at 100% && it produced some decent flushes. But when I went to dump it I would find a uncolonized patch of sub on the bottom.. lol Now I consolidate my tubs for 3-5 days prior to applying the casing. Remember, we are talking about a completely different genus. Panaeolus mycelia is tomentose, frail && fragile, compared to Psilocybe Cubensis which throws out ropey rhizomorphic mycelia.
-------------------- Giving is all we have, for we're just grateful to be alive

Edited by Fantastic Mr. Fox (12/18/15 08:00 PM)
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Quote:
Fantastic Mr. Fox said:
Quote:
Psilosoulful said: It's personal preference really. The sub will pin up when it is fully colonized inside anyway, So giving it the extra couple of days with the holes covered isn't gonna be a big deal..
Not true, Ive had a few pan subs that I cased/fruited at 100% && it produced some decent flushes. But when I went to dump it I would find a uncolonized patch of sub on the bottom.. lol Now I consolidate my tubs for 3-5 days prior to applying the casing. Remember, we are talking about a completely different genus. Panaeolus mycelia is tomentose, frail && fragile, compared to Psilocybe Cubensis which throws out ropey rhizomorphic mycelia.
Yeah, that's definitely true. I can see why that would help, what is the average time for sub colonization given a depth of 2"? I'm on day 5 with my mini mono and the surface is almost completely colonized.
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Fantastic Mr. Fox
Grass Seed Connoisseur


Registered: 02/12/15
Posts: 3,225
Loc:
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Re: Official Copelandia/Panaeolus Thread [Re: Psilosoulful]
#22673551 - 12/18/15 08:10 PM (8 years, 1 month ago) |
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The surface should have been 100% by now, IME
What was your spawn/sub ratio? Pics?
-------------------- Giving is all we have, for we're just grateful to be alive

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dusttodust

Registered: 11/26/12
Posts: 491
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Im speaking from my experience. Belive what u want
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Fantastic Mr. Fox
Grass Seed Connoisseur


Registered: 02/12/15
Posts: 3,225
Loc:
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Re: Official Copelandia/Panaeolus Thread [Re: dusttodust]
#22673808 - 12/18/15 09:17 PM (8 years, 1 month ago) |
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Pics referencing what you speak?
-------------------- Giving is all we have, for we're just grateful to be alive

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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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 Substrate composed of 3 quarts of (50/50 manure, verm, and a half cup of gypsum). 3 quarts of spawn used (100% colonized WBS). Sub depth is about 2". The mix was properly pasteurized.
Edited by Psilosoulful (12/18/15 09:22 PM)
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Fantastic Mr. Fox
Grass Seed Connoisseur


Registered: 02/12/15
Posts: 3,225
Loc:
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Re: Official Copelandia/Panaeolus Thread [Re: Psilosoulful]
#22674060 - 12/18/15 10:17 PM (8 years, 1 month ago) |
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Ok, so you used a 1:1 ratio.
You use poly for the spawn run? Or is that in fruiting conditions?
The poly could probably be contributing to the somewhat sluggish colonization times.
Everything looks good though!
-------------------- Giving is all we have, for we're just grateful to be alive

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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Really? I thought the poly wouldn't be a huge factor in colonization times. I was checking out Azur's thread on stuffing vs covering holes at spawning, I figured it would work the same for pans?
EDIT: Decided to go ahead and tape up those bottom holes anyway. The uneven lid should provide enough GE.
Day 5

Edited by Psilosoulful (12/18/15 11:31 PM)
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dusttodust

Registered: 11/26/12
Posts: 491
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  1st plate is slanted
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Teemo 6T3
႟тнe мedιcιne мan ☼



Registered: 07/21/14
Posts: 1,570
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Re: Official Copelandia/Panaeolus Thread [Re: dusttodust]
#22674811 - 12/19/15 04:33 AM (8 years, 1 month ago) |
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Hey guys, seems today that some of the agar that was transferred to grain is fluffing up pan mycelium, I'll take pictures in a day or so just so it spreads.
Btw i think dusttodust might be right, i think its something to do with the agar recipe, making the mycelium go down the surface of agar for some reason (maybe uneven distribution of nutes, since I'm using potato flakes).
Because I've had my fair share of bacteria, which produces a murky looking liquid on top of agar. I think the pictures weren't taken properly because in real life it doesn't look like bacteria, it just seems like the mycelium went down the surface of the agar which make it seem like uneven mycelium..
We'll tell in a few days i guess lol, this is kinda interesting
-------------------- Shrooming Is Of The Essence   Rest In Peace Dankington
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Re: Official Copelandia/Panaeolus Thread [Re: Teemo 6T3]
#22675364 - 12/19/15 11:15 AM (8 years, 1 month ago) |
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Quote:
Teemo 6T3 said: Btw i think dusttodust might be right, i think its something to do with the agar recipe, making the mycelium go down the surface of agar for some reason (maybe uneven distribution of nutes, since I'm using potato flakes).
What was your recipe? I always use the standard recipe included in the pasty plate videos. Never had a problem yet. Pan mycelium takes a while to get going, but when it does it performs well.
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