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OfflineAsuraS
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Registered: 08/01/11
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Re: Official Copelandia/Panaeolus Thread [Re: High Night Expanse] * 1
    #26170495 - 09/05/19 11:49 AM (4 years, 4 months ago)

@Nakoa I would grow that


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OfflineAdas
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Re: Official Copelandia/Panaeolus Thread [Re: Nesmaster01]
    #26170501 - 09/05/19 11:51 AM (4 years, 4 months ago)

Quote:

Nesmaster01 said:
So I have some 2 year old pan spores and tried germinating on agar on multiple plates but I cant get anything but GD contams. Should I make some antibiotic agar plates? Ive tried 2x in front of my flow hood with inoc loop.

I am pretty sure its a mix of old spores and shitty prints. They are bagged well but looks like they were printed on some tyvec. Fucking Reddit...




If they're old they're old. Nothing you can do to save them.


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OfflineJakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: Adas]
    #26170529 - 09/05/19 12:11 PM (4 years, 4 months ago)

You can try using venom reagent to get germ. (Worked for sum caerulescens & gallaeciae that I could not get to germ traditionally)


--------------------
Natural omt/detox
online  pant cult classes available last Saturday of every month go to buymeacoffee.com/jakeoncid to sign up (1 on 1 consultations also available
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OfflineHigh Night Expanse
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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
    #26170540 - 09/05/19 12:20 PM (4 years, 4 months ago)

Quote:

Jakeoncid419 said:
You can try using venom reagent to get germ. (Worked for sum caerulescens & gallaeciae that I could not get to germ traditionally)




Your mood status is on point. :grin:


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OfflineJakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: High Night Expanse]
    #26170566 - 09/05/19 12:35 PM (4 years, 4 months ago)

Lol indeed. All the best ppl are lol


--------------------
Natural omt/detox
online  pant cult classes available last Saturday of every month go to buymeacoffee.com/jakeoncid to sign up (1 on 1 consultations also available
JOC PAN TEK
CORDYCEPS MILITARIS

EXOTICS
[


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OfflineShaperDreaming
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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
    #26170880 - 09/05/19 02:58 PM (4 years, 4 months ago)

OK, back after 2.5 months of starting back over. You can see what I did before in my Journal.

Pan myc is both more finiky, and grows slower than cubes. Not a major complaint, but it took me a little longer to get back to this point than I expected. So I adjusted my recipe slightly:

First thing is I switched over to wide-mouth half-pint jars. This caused me to reduce my recipe greatly for the 8 jars I used.


They're hilariously small. I filled them about halfway with the below recipe:

Quote:

ShaperDreaming said:
  • 1c Verm
  • 1c Manure/Hummus
  • 1c Water
  • 1/2c Brown Rice Flour (BRF)
  • 1/2c Ground rye grass seed (RGS)
  • 2Tbs Coffee
  • 1tsp Gypsum






The recipe is only slightly modified, basically to remove the coffee and reduce the volume. I then PC'd it for a while (longer than necessary for a few reasons, but ... we'll just say over 2 hours :wink: ).

I mixed in colonized oats at a rate of about 1:2~ (hard to tell at these sizes) and shook them up:



So, we'll see if it recovers! If you're wondering about the two different lid types, I'm just fucking around with them. I want to ensure that at least some of these will work if the new lids fail, so I did 50/50. These are the new "leak proof" plastic lids from Ball. They are PP5, but they do not have a gasket. The inside of the lid where the lip goes has another lip itself that the jar fits right into. It's very solid/cool, but I have lost most of my faith in plastic... but I hate 2-piece lids more.


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OfflineDabadoodledoo710
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Re: Official Copelandia/Panaeolus Thread [Re: High Night Expanse]
    #26171120 - 09/05/19 04:56 PM (4 years, 4 months ago)

Good enough for grain?



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OfflineAsuraS
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Re: Official Copelandia/Panaeolus Thread [Re: Dabadoodledoo710]
    #26171130 - 09/05/19 05:01 PM (4 years, 4 months ago)

Quote:

Dabadoodledoo710 said:
Good enough for grain?






I'd use it


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OfflineDabadoodledoo710
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Re: Official Copelandia/Panaeolus Thread [Re: Asura]
    #26171146 - 09/05/19 05:10 PM (4 years, 4 months ago)

Excellent! Thanks


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OfflineAsuraS
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Re: Official Copelandia/Panaeolus Thread [Re: Dabadoodledoo710] * 1
    #26171252 - 09/05/19 06:18 PM (4 years, 4 months ago)

Well dudes just wanted to say that I haven't been active much because I have been studying for
a job at Amazon Web Services...it's a lot of shit to go over. And I am out all next week to go
backpacking in Montana.

But I will be back soon with some new grows.

Keep rocking those pans :rockon:


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OfflineMacMerdin
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Re: Official Copelandia/Panaeolus Thread [Re: Dabadoodledoo710]
    #26171273 - 09/05/19 06:35 PM (4 years, 4 months ago)

Quote:

Dabadoodledoo710 said:
Good enough for grain?






Looks good.


--------------------
MacMerdin's Simple Pan-Cakes



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InvisibleSunnyDayze
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Re: Official Copelandia/Panaeolus Thread [Re: Asura]
    #26171425 - 09/05/19 08:20 PM (4 years, 4 months ago)

Quote:

Asura said:
Well dudes just wanted to say that I haven't been active much because I have been studying for
a job at Amazon Web Services...it's a lot of shit to go over. And I am out all next week to go
backpacking in Montana.

But I will be back soon with some new grows.

Keep rocking those pans :rockon:





Good luck Asura and have fun backpacking!

I took my pan culture out of the fridge to get ready to grow again and it's not doing well :mad:  I lost several prints, my ps.cyan is awesome though! I think they must have froze.  Back to prints again, have to get my shit going dang it.


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OfflineNesmaster01
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Re: Official Copelandia/Panaeolus Thread [Re: Asura]
    #26171859 - 09/06/19 05:21 AM (4 years, 4 months ago)

Quote:

Asura said:
Quote:

Nesmaster01 said:
So I have some 2 year old pan spores and tried germinating on agar on multiple plates but I cant get anything but GD contams. Should I make some antibiotic agar plates? Ive tried 2x in front of my flow hood with inoc loop.

I am pretty sure its a mix of old spores and shitty prints. They are bagged well but looks like they were printed on some tyvec. Fucking Reddit...




I make a swab with a sterile q-tip and streak using this method:
https://microbeonline.com/streak-plate-method-principle-purpose-procedure-results/

Basically, I'll use one swab to streak 4 plates. On the 3rd or 4th plate is where I usually get
discrete colonies of mycelium that I can transfer away. The T1 plates from these are usually very
uniform and clean.

Maybe give something like this a try and you might be able to work around the contams.




Yeah, I tried that method first with some pre sterilized swabs, though it might have been the swabs or my SP, tried again with a flamed inoc loop as well. within 2-3 days the agar goes bacterial everywhere i swipe on the agar plates, whether it was a flamed inoc loop or the sterile swab. I know my procedure is good because I have 4 strains of cubes under my belt. I think I just have some crap prints or they are too old at 2 years? =(


--------------------
"Life is really simple, but we insist on making it complicated."
“I hear and I forget. I see and I remember. I do and I understand.”
“When it is obvious that the goals cannot be reached, don’t adjust the goals, adjust the action steps.”
– Confucius


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OfflineGeinstein Happy Birthday!
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Re: Official Copelandia/Panaeolus Thread [Re: Nesmaster01]
    #26172072 - 09/06/19 08:39 AM (4 years, 4 months ago)

Anyone ever knocked up straight jar of sterilized Hpoo and spawned it and it worked?


--------------------


Nothing breads nothing


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OfflineShaperDreaming
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Re: Official Copelandia/Panaeolus Thread [Re: Geinstein]
    #26172074 - 09/06/19 08:41 AM (4 years, 4 months ago)

I could see it working with LC as the inoculation method since it has nutes. Probably slow af.


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OfflineJakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: Geinstein]
    #26172175 - 09/06/19 09:37 AM (4 years, 4 months ago)

Quote:

Geinstein said:
Anyone ever knocked up straight jar of sterilized Hpoo and spawned it and it worked?



Yup did it a while back it worked ok but did take a much longer time & didn’t get a canopy


--------------------
Natural omt/detox
online  pant cult classes available last Saturday of every month go to buymeacoffee.com/jakeoncid to sign up (1 on 1 consultations also available
JOC PAN TEK
CORDYCEPS MILITARIS

EXOTICS
[


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OfflineHigh Night Expanse
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Registered: 11/07/18
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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419] * 4
    #26172714 - 09/06/19 02:38 PM (4 years, 4 months ago)



spawned  08.25.19



cased 08.29.19





12 days after spawning  :flyhigh:  :jointsmile:


Edited by High Night Expanse (09/06/19 02:43 PM)


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OfflineAdas
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Re: Official Copelandia/Panaeolus Thread [Re: High Night Expanse]
    #26172744 - 09/06/19 02:51 PM (4 years, 4 months ago)

You gotta be kidding me dude! These are not only beautiful, but also huge!

What variety is that?


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OfflineJakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: Adas]
    #26172877 - 09/06/19 04:06 PM (4 years, 4 months ago)

Nice outdoor pan grow high night! I’ve only ever gotten a few small outdoor fruits. They always get before fully growin. Very well done! Did you tend it daily?

Here’s sum pan trays


--------------------
Natural omt/detox
online  pant cult classes available last Saturday of every month go to buymeacoffee.com/jakeoncid to sign up (1 on 1 consultations also available
JOC PAN TEK
CORDYCEPS MILITARIS

EXOTICS
[


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OfflineKapnDank
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Re: Official Copelandia/Panaeolus Thread [Re: High Night Expanse]
    #26172920 - 09/06/19 04:36 PM (4 years, 4 months ago)

Quote:

High Night Expanse said:


spawned  08.25.19



cased 08.29.19





12 days after spawning  :flyhigh:  :jointsmile:



What was the substrate? I tried an outdoor bed earlier this year with wbs spawned to straight hpoo and it started colonizing well then just up and died. Thinking it may have not been fluffy enough and choked out. Idk it was my first and only pan attempt outdoors


--------------------
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