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Psilosopherr
A psilly goose



Registered: 02/15/12
Posts: 12,278
Last seen: 1 month, 10 days
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Re: Official Copelandia/Panaeolus Thread [Re: the_r3dz]
#22662505 - 12/16/15 10:28 AM (8 years, 1 month ago) |
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same, I think my spores are just bunk. I'm not quick to blame to source material either, must've tried 20 or more plates by now.
Had a couple plates with what I thought was healthy pan myc. Cooked up some agar, transferred it to three new plates. Smell the source jar afterward and 
on to cambog. forget cyan for now, time for new source material.
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the_r3dz
Trich Propagator


Registered: 03/26/15
Posts: 5,709
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Re: Official Copelandia/Panaeolus Thread [Re: Psilosopherr]
#22662708 - 12/16/15 11:26 AM (8 years, 1 month ago) |
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Yeah I made the syringes myself from a print so I'm not too surprised they didn't work out, never done it before, didn't feel like I knew too much what I was doing. So I've got some vendor spores coming my way now, hopefully those will be much better.
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Fantastic Mr. Fox
Grass Seed Connoisseur


Registered: 02/12/15
Posts: 3,225
Loc:
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Re: Official Copelandia/Panaeolus Thread [Re: the_r3dz]
#22663523 - 12/16/15 03:17 PM (8 years, 1 month ago) |
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Shoot a  I've got you on a 90% guaranteed sterile print. I recommend starting on agar, but I'm sure they're clean enough to go straight into suspension (syringe)
-------------------- Giving is all we have, for we're just grateful to be alive

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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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trying to get my bali cleaned up but i went 5 transfers so far with little luck, still a bit of bacteria and mold showing..may just put the bali to rest
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Fantastic Mr. Fox
Grass Seed Connoisseur


Registered: 02/12/15
Posts: 3,225
Loc:
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Re: Official Copelandia/Panaeolus Thread [Re: cronicr]
#22665618 - 12/16/15 11:09 PM (8 years, 1 month ago) |
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Nooo I can help if ya' want
Seriously though, I'm going to need you to stay on top of tht. I've been waiting for some Bali grows in the cult. I'm sure you can get it, stay persistent cron.
Hmm... have you tried an antibacterial agar recipe?
-------------------- Giving is all we have, for we're just grateful to be alive

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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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i'll probably be looking at an isolate by the time i'm done lol!, bacteria doesn't worry me it's this mold that seems to be riding it, i'll see what happens though
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Teemo 6T3
႟тнe мedιcιne мan ☼



Registered: 07/21/14
Posts: 1,570
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Re: Official Copelandia/Panaeolus Thread [Re: cronicr]
#22666526 - 12/17/15 06:24 AM (8 years, 1 month ago) |
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Damn, this mofo is eating through the agar..
Gonna be transferring to grain today, looks clean as shit..

-------------------- Shrooming Is Of The Essence   Rest In Peace Dankington
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Fantastic Mr. Fox
Grass Seed Connoisseur


Registered: 02/12/15
Posts: 3,225
Loc:
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Re: Official Copelandia/Panaeolus Thread [Re: Teemo 6T3]
#22666630 - 12/17/15 07:38 AM (8 years, 1 month ago) |
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That does not look clean to me
Not sure what it is, but something's going on with tht culture.. I want to say bacteria, but I can't tell with certainty.
Can you provide better photos?
-------------------- Giving is all we have, for we're just grateful to be alive

Edited by Fantastic Mr. Fox (12/17/15 07:43 AM)
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Quote:
Not sure what it is, but something's going on with tht culture..
^^^this. that plate is strange brew
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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Teemo 6T3
႟тнe мedιcιne мan ☼



Registered: 07/21/14
Posts: 1,570
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Re: Official Copelandia/Panaeolus Thread [Re: blindingleaf]
#22666770 - 12/17/15 08:51 AM (8 years, 1 month ago) |
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Fuck, and here i thought it's clean 
I'll take better pictures ASAP, lemme just get my SAB ready.
-------------------- Shrooming Is Of The Essence   Rest In Peace Dankington
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Teemo 6T3
႟тнe мedιcιne мan ☼



Registered: 07/21/14
Posts: 1,570
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Re: Official Copelandia/Panaeolus Thread [Re: Teemo 6T3]
#22667027 - 12/17/15 10:13 AM (8 years, 1 month ago) |
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Here you go guys, I hope its good, as i remember those weak patches are where the contaminant is right or is it clean? The grains are already in the PC getting ready 

-------------------- Shrooming Is Of The Essence   Rest In Peace Dankington
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Official Copelandia/Panaeolus Thread [Re: Teemo 6T3]
#22667080 - 12/17/15 10:25 AM (8 years, 1 month ago) |
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i think i am more paranoid than most, so wait for fox, cause he deals with pans more than i do by a long shot.
just wondering why u don't do one wedge per plate? or is that a spore swipe plate?
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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Mr.PhilCybin
Master Baiter


Registered: 06/13/11
Posts: 11,642
Loc: Gnarnia
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Re: Official Copelandia/Panaeolus Thread [Re: blindingleaf]
#22667087 - 12/17/15 10:27 AM (8 years, 1 month ago) |
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the dark spots look funky to me, but there's definitely good tissue to pull from it.
my 2 cents anyways.
the pan myc is a lot lighter and fluffier than cube myc so I always get more paranoid than I should.
it shouldn't be greyish though
-------------------- I'm stupid, Falcon91Wolvrn03 is smart. I'm ugly, Falcon91Wolvrn03 is beautiful. I'm a loser, Falcon91Wolvrn03 is a winner. Someday, I hope to be like Falcon91Wolvrn03 but secretly know I never will.
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Teemo 6T3
႟тнe мedιcιne мan ☼



Registered: 07/21/14
Posts: 1,570
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Re: Official Copelandia/Panaeolus Thread [Re: blindingleaf]
#22667116 - 12/17/15 10:33 AM (8 years, 1 month ago) |
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It's from a syringe that I suspected to be contaminated so i dropped on agar, looks kinda clean to me, yet I'm still new with Pans so ye 
Hopefully it isn't a contam cus my Rye jars are getting ready
-------------------- Shrooming Is Of The Essence   Rest In Peace Dankington
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Mr.PhilCybin
Master Baiter


Registered: 06/13/11
Posts: 11,642
Loc: Gnarnia
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Re: Official Copelandia/Panaeolus Thread [Re: Teemo 6T3]
#22667134 - 12/17/15 10:37 AM (8 years, 1 month ago) |
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just make a couple transfers and you'll know for sure.
I like to smell the plate I'm tossing after I make a transfer. you can be pretty sure how the new wedge is with a simple sniff.
there's def. good tissue to pull there though
-------------------- I'm stupid, Falcon91Wolvrn03 is smart. I'm ugly, Falcon91Wolvrn03 is beautiful. I'm a loser, Falcon91Wolvrn03 is a winner. Someday, I hope to be like Falcon91Wolvrn03 but secretly know I never will.
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Teemo 6T3
႟тнe мedιcιne мan ☼



Registered: 07/21/14
Posts: 1,570
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Re: Official Copelandia/Panaeolus Thread [Re: Mr.PhilCybin]
#22667175 - 12/17/15 10:46 AM (8 years, 1 month ago) |
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It actually smelled kinda sweet before i closed it. Where would you suggest transferring from, because i hate transfers  I tend to be unlucky and sector crappy genetics that don't even fruit
-------------------- Shrooming Is Of The Essence   Rest In Peace Dankington
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Re: Official Copelandia/Panaeolus Thread [Re: Teemo 6T3]
#22667377 - 12/17/15 11:37 AM (8 years, 1 month ago) |
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Quote:
Teemo 6T3 said:
 
I would take a small wedge from the very top of the plate to clean it up further. Don't worry about crappy genetics with pans, they are almost always consistently strong at the 1 gram range (dried), so no worries there.
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Teemo 6T3
႟тнe мedιcιne мan ☼



Registered: 07/21/14
Posts: 1,570
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Re: Official Copelandia/Panaeolus Thread [Re: Psilosoulful]
#22667386 - 12/17/15 11:40 AM (8 years, 1 month ago) |
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The far leading one on the 2 o'clock position?
-------------------- Shrooming Is Of The Essence   Rest In Peace Dankington
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Re: Official Copelandia/Panaeolus Thread [Re: Teemo 6T3]
#22667410 - 12/17/15 11:47 AM (8 years, 1 month ago) |
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More like at the 12 o'clock position. The growth seems less whispy in that area. You may need to clean it up twice just to be sure.
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GlitchInSpace
Glitch In Space


Registered: 11/24/13
Posts: 426
Loc: Texas
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Re: Official Copelandia/Panaeolus Thread [Re: Teemo 6T3]
#22667416 - 12/17/15 11:50 AM (8 years, 1 month ago) |
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agreed, i would totally make some transfers before inoculating your grains.
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