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ukshroomer
Law Abider


Registered: 11/08/09
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Re: Official Copelandia/Panaeolus Thread [Re: Psilosoulful]
#22635839 - 12/09/15 08:18 PM (8 years, 1 month ago) |
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i shook them already.. is it possible to shake twice or am i risking a stallout ?
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Fantastic Mr. Fox
Grass Seed Connoisseur


Registered: 02/12/15
Posts: 3,225
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Re: Official Copelandia/Panaeolus Thread [Re: ukshroomer]
#22635852 - 12/09/15 08:20 PM (8 years, 1 month ago) |
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If you've shaken them already leave them be.
-------------------- Giving is all we have, for we're just grateful to be alive

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ukshroomer
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Post deleted by ukshroomer
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Fantastic Mr. Fox
Grass Seed Connoisseur


Registered: 02/12/15
Posts: 3,225
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Re: Official Copelandia/Panaeolus Thread [Re: ukshroomer]
#22649394 - 12/13/15 09:48 AM (8 years, 1 month ago) |
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Bacteria..
I would start over on agar.
-------------------- Giving is all we have, for we're just grateful to be alive

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ukshroomer
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Registered: 11/08/09
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Post deleted by ukshroomer
Reason for deletion: old
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Fantastic Mr. Fox
Grass Seed Connoisseur


Registered: 02/12/15
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Re: Official Copelandia/Panaeolus Thread [Re: ukshroomer]
#22649521 - 12/13/15 10:34 AM (8 years, 1 month ago) |
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Much better! 
I would expand those by G2G after 100% colonization has been reached.. Jus a suggestion
-------------------- Giving is all we have, for we're just grateful to be alive

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ukshroomer
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Registered: 11/08/09
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I am definitely going to do that. I have 4 jars (including this one) that seem healthy. This is out of ten that i inoculated with a spore mate vial. I was hoping for better results but I am still happy knowing that now I will have some spawn to continue with.
With one I am going to do g2g. With the other two I am going spawn them in a glovebox to manure, straw, verm , gypsum substrate (sterilized in a large jar) ). With the last one I am going spawn it to the same substrate mentioned above only pasteurized. By the way the above pics are all of the same jar.
Also do you think the bacteria was from a contaminated syringe or from the grains being too wet. I also made 20 jars the same day and in the same way and inoculated them with cubensis mexican and only one of them got contamination. Is pan mycelium more sensitive to grain water content?
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Canaderp
I'm tryin'


Registered: 11/26/15
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Re: Official Copelandia/Panaeolus Thread [Re: ukshroomer]
#22649903 - 12/13/15 12:38 PM (8 years, 1 month ago) |
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ukshroomer Nice!
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Teemo 6T3
႟тнe мedιcιne мan ☼



Registered: 07/21/14
Posts: 1,570
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Re: Official Copelandia/Panaeolus Thread [Re: Canaderp]
#22650132 - 12/13/15 01:59 PM (8 years, 1 month ago) |
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Hey guys i inoculated this jar on the 14th of November (4 weeks ago)
they started looking fine like any other Pan. cam mycelium but the top and side parts turned out to look very fishy, either a very weird contaminant or very weird bruising, what do you guys think this is  (the bottom of the jar has nice clean growth)..
The jars were sterilized at 15 psi for 90 minutes, the filter is a 0.22 micro meter SFD.

And i tested if the syringe is clean by dropping a bit on agar ( which apparently looks clean and healthy growth) And should i transfer this agar to grain now? what do you guys think?
-------------------- Shrooming Is Of The Essence   Rest In Peace Dankington
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Moldy thumb
Necromancer



Registered: 08/05/15
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Re: Official Copelandia/Panaeolus Thread [Re: Teemo 6T3]
#22650366 - 12/13/15 03:09 PM (8 years, 1 month ago) |
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Does this look like pan mycelium to you guys?
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cronicr



Registered: 08/07/11
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Re: Official Copelandia/Panaeolus Thread [Re: Moldy thumb]
#22650589 - 12/13/15 04:06 PM (8 years, 1 month ago) |
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yep
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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District
Strange



Registered: 07/12/15
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Re: Official Copelandia/Panaeolus Thread [Re: cronicr]
#22651617 - 12/13/15 08:01 PM (8 years, 1 month ago) |
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Does drilling the lid of my jar and placing micropore tape enough for gas exchange? I'm trying to make grain spawn.
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cronicr



Registered: 08/07/11
Posts: 61,436
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Re: Official Copelandia/Panaeolus Thread [Re: District]
#22651624 - 12/13/15 08:03 PM (8 years, 1 month ago) |
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it's enough for ge, i've done about 100 jars with just micropore tape but did lose a few...i would use polyfil or sfd's, micropore works but is fragile in comparison
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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GlitchInSpace
Glitch In Space


Registered: 11/24/13
Posts: 426
Loc: Texas
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Re: Official Copelandia/Panaeolus Thread [Re: cronicr]
#22654031 - 12/14/15 01:30 PM (8 years, 1 month ago) |
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damn, i was totally looking forward to the cinctulus grow. i may need to bring out my cinctulus print. i've got some pans that i just put to grains
 1st transfer
Edited by GlitchInSpace (12/17/15 08:29 PM)
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Psilosoulful

Registered: 09/05/14
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Re: Official Copelandia/Panaeolus Thread [Re: GlitchInSpace]
#22654170 - 12/14/15 02:22 PM (8 years, 1 month ago) |
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Looks like nice clean growth, hopefully it colonizes the grains fast, good luck!
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Teemo 6T3
႟тнe мedιcιne мan ☼



Registered: 07/21/14
Posts: 1,570
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Re: Official Copelandia/Panaeolus Thread [Re: Psilosoulful]
#22657707 - 12/15/15 07:49 AM (8 years, 1 month ago) |
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Feeling left out
-------------------- Shrooming Is Of The Essence   Rest In Peace Dankington
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the_r3dz
Trich Propagator


Registered: 03/26/15
Posts: 5,709
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Re: Official Copelandia/Panaeolus Thread [Re: Teemo 6T3]
#22658632 - 12/15/15 01:38 PM (8 years, 1 month ago) |
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I inocculated this bitch right before I left for 4 weeks, I made up a whole bunch but this is the only one that's showed any kind of growth.
It doesn't look right to me, but do you of you see any panaeolus growth?
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Teemo 6T3
႟тнe мedιcιne мan ☼



Registered: 07/21/14
Posts: 1,570
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Re: Official Copelandia/Panaeolus Thread [Re: the_r3dz]
#22658675 - 12/15/15 01:50 PM (8 years, 1 month ago) |
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Looks good to me, except those yellowish colours in the middle, i'd say just transfer away from those parts. (The 2 last picture IMO looks too fishy though, top left and bottom left; or it might be the camera, if its fluffy kinda wispy white mycelium you're good)
But i'd wait for a more experienced reply from the others, i just started Pans recently.
-------------------- Shrooming Is Of The Essence   Rest In Peace Dankington
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Psilosoulful

Registered: 09/05/14
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Re: Official Copelandia/Panaeolus Thread [Re: the_r3dz]
#22658866 - 12/15/15 02:42 PM (8 years, 1 month ago) |
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Quote:
the_r3dz said:

Those are all contaminated. Seems like the bacteria is surrounding the entire plate. Your best bet is to work on your sterile procedures and knock up fresh plates.
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the_r3dz
Trich Propagator


Registered: 03/26/15
Posts: 5,709
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Re: Official Copelandia/Panaeolus Thread [Re: Psilosoulful]
#22661258 - 12/15/15 10:43 PM (8 years, 1 month ago) |
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Pretty sure it's the syringe, made a ton of plates with it at this point to no avail with any of them. Going to just swab the print next time.
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