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OfflineAsuraS
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Re: Official Copelandia/Panaeolus Thread [Re: Rad Aghast]
    #26019410 - 05/28/19 09:00 PM (4 years, 7 months ago)

Looks awesome, Rad. I haven't had a good mover like that in a bit.


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Re: Official Copelandia/Panaeolus Thread [Re: Asura]
    #26019446 - 05/28/19 09:22 PM (4 years, 7 months ago)

Quote:

Asura said:
Quote:

mushboy said:
I got some pan LC going from those spores(went to agar) but I think its contained. Hard to tell...

Usually LCs do not have sattalite growth unless the wedge flakes off myc fragments:shrug:




You got a pic?




fucking auto correct on my fone. my lc is contained folks. everything is ok:facepalm:

CONTAMINATED:shake:



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OfflineAsuraS
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Re: Official Copelandia/Panaeolus Thread [Re: mushboy]
    #26019452 - 05/28/19 09:25 PM (4 years, 7 months ago)

I can't tell if it is from that pic, man :shrug:

Looks like a whole lot of fluffy white to me.

What am I missing?

Edit: are you worried bc it grew out fast, because my shit is ready to go in
five days usually.


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OfflineJakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: Asura]
    #26019455 - 05/28/19 09:26 PM (4 years, 7 months ago)

Put it to agar and test it


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Re: Official Copelandia/Panaeolus Thread [Re: Asura]
    #26019458 - 05/28/19 09:27 PM (4 years, 7 months ago)

right in the center. the brownish speck is not on the glass its inside the culture. you can almost make out a ring around the brown shit:shrug:

maybe im wrong. id be thrilled:lol: only one way to find out ...:uhoh:

ill test it on grains. i dont want to suck up lc into syringes:thumbdown:


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OfflineAsuraS
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Re: Official Copelandia/Panaeolus Thread [Re: mushboy]
    #26019462 - 05/28/19 09:30 PM (4 years, 7 months ago)

Oh shit, I am blind as a bat. Yeah, that looks off. But I have to ask, what is
your LC recipe?

I thought I was getting contams for a while, but it was actually the LME coming out of
solution and clumping up on the side of the jar.


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OfflineJakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: mushboy]
    #26019464 - 05/28/19 09:31 PM (4 years, 7 months ago)

Quote:

mushboy said:
right in the center. the brownish speck is not on the glass its inside the culture. you can almost make out a ring around the brown shit:shrug:

maybe im wrong. id be thrilled:lol: only one way to find out ...:uhoh:

ill test it on grains. i dont want to suck up lc into syringes:thumbdown:



How come? I do it all the time (it’s easy with a stir plate) without one I would get clogs sometimes but I could always get it eventually


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OfflineJakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
    #26019467 - 05/28/19 09:31 PM (4 years, 7 months ago)

Oh I thought that was on the outside of the jar


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Re: Official Copelandia/Panaeolus Thread [Re: Asura]
    #26019468 - 05/28/19 09:32 PM (4 years, 7 months ago)

thats what i thought at first too but i havent had clumping in a long time.

whatever is growing should be ready soon tho:popcorn:


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OfflineRad Aghast
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Re: Official Copelandia/Panaeolus Thread [Re: mushboy]
    #26019473 - 05/28/19 09:35 PM (4 years, 7 months ago)

Quote:

mushboy said:
right in the center. the brownish speck is not on the glass its inside the culture. you can almost make out a ring around the brown shit:shrug:

maybe im wrong. id be thrilled:lol: only one way to find out ...:uhoh:

ill test it on grains. i dont want to suck up lc into syringes:thumbdown:




I'm curious why you don't use syringes to drop your LC. Is it because you're not using sterile syringes?

Edit: I see jakes reply - assuming you get clogging.


Edited by Rad Aghast (05/28/19 09:38 PM)


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Re: Official Copelandia/Panaeolus Thread [Re: Rad Aghast]
    #26019481 - 05/28/19 09:40 PM (4 years, 7 months ago)

making the lc syringe itself could contminate the inoculate. knowing my dumbass ill for sure contaminate so why bother:shrug:

lc is basically the same thing as agar. would you suck agar out of a media bottle into a syringe to make petris?
:liottalol:


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OfflineJakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: mushboy]
    #26019488 - 05/28/19 09:45 PM (4 years, 7 months ago)

Honestly I don’t really like using LC because I don’t see where it saves time I like being able to see my culture on the dish.  Plus I do a lot of genetics hunting so I have tons of dishes anyway it just doesn’t make sense to do LC  (Except for cordyceps) I have made a couple though for pans and sucked it up with a syringe tested it on agar. But I also the same night I hit my dish sucked up several syringes and wrap them in saran wrap and place in the refrigerator  that way I’m getting the same LC that I tested. It worked fine just didn’t save me any time and actually my jars colonize much slower than they do if I have a healthy inoculation nute dish.


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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
    #26019509 - 05/28/19 09:57 PM (4 years, 7 months ago)

i saw the culture on the dish that went into the broth:shrug:


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Re: Official Copelandia/Panaeolus Thread [Re: mushboy]
    #26019518 - 05/28/19 10:02 PM (4 years, 7 months ago)

Yeah I know, I mean I get how it can be convenient depending on situation, but I have so many dishes i still end up tossing sun good dishes and sometimes if they are the same iso I will put 2 or even 3 dishes in a jar to use em up lol so there is no benefit for me. But most ppl don’t have thT many dishes I would imagine


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OfflineRad Aghast
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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
    #26019529 - 05/28/19 10:08 PM (4 years, 7 months ago)

hmmm - interesting comparison. this may just be a matter of style. I have been going the route of inoculating bulk substrate in bags.

however, when I do plates I pour from a sterile bottle into sterile plates. I use the whole bottle. I don't store it for later. Once its opened its compromised so I use the whole thing.

when I do LC I aspirate with a sterile needle/syringe and inject through a port into sterile bags. I tend to re-use an LC several times before its gone and I have never had a batch compromised if I follow this procedure.

I'm not sure how one would do a sterile x-fer of LC to a sealed sterile bag of substrate without using a syringe/needle.


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Re: Official Copelandia/Panaeolus Thread [Re: Rad Aghast]
    #26019534 - 05/28/19 10:11 PM (4 years, 7 months ago)

Pour it in the top in a FH
Well you would have to seal it after the pressure cooker but I do all my bags that way I never pre-seal if you fold them properly they basically seal them selves anyway
Sometimes if they’re over filled because I have a lot of substrate left but not enough for two bags I will fold them over then use electrical tape to hold them in place just to pieces but use a long strip of them because it is going to stretch but the electrical tape works perfect in the pressure cooker it comes right off when you’re done at stretches when it needs to and it doesn’t leave a bunch of adhesive on your bag but like I said this is only necessary if your bag is so full you can’t get the proper fold


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Edited by Jakeoncid419 (05/28/19 10:15 PM)


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Re: Official Copelandia/Panaeolus Thread [Re: Rad Aghast]
    #26019548 - 05/28/19 10:20 PM (4 years, 7 months ago)

or do it in a tall sab but its not practical and i couldnt do it consistently:sad:

Quote:

Rad Aghast said:
Once its opened its compromised so I use the whole thing.







if it were compromised then you would contaminate. you should be able to seal that bottle back up and let it sit. it the bottle molds then your technique is lacking imo:blush:


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OfflineJakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: mushboy] * 1
    #26020228 - 05/29/19 10:00 AM (4 years, 7 months ago)

Pan cam Indonesia gonna clone the big guys. Should result in a lighting fast iso


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OfflineHigh Night Expanse
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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
    #26020561 - 05/29/19 01:54 PM (4 years, 7 months ago)

Great... set out to do some cubes and shitake.  Couldnt help myself and chanced innoculating the invitro pin clone. It got dropped on its head! Dropped right out of the jar as im trying to cut a wedge and onto the floor of my still air box. Right into a pool of water/dishliquid/peroxide.  T.T

Still inoculated with it, the whole thing instead of a wedge to see what ends up happening.


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Re: Official Copelandia/Panaeolus Thread [Re: High Night Expanse]
    #26020623 - 05/29/19 02:20 PM (4 years, 7 months ago)

Is that pan related? Lol,  and shiitake has 2 i's in it... I am glad you didn't put 2 t's instead lol


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