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mullugh
Stranger

Registered: 03/26/18
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Re: Official Copelandia/Panaeolus Thread [Re: Asura]
#26013003 - 05/25/19 04:04 PM (4 years, 8 months ago) |
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I know it's not pans (a string of failures), but I've somehow managed to isolate a PE culture with tiny pathetic fruits. Back to the drawing board.
Good news is my pan LC seems to have gone well. Two days after inoculating some jars and no signs of life yet...
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345536345
Pan parent!



Registered: 08/13/17
Posts: 268
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Re: Official Copelandia/Panaeolus Thread [Re: mullugh]
#26013083 - 05/25/19 05:12 PM (4 years, 8 months ago) |
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I must say (as I'm struggling once again to get my first pan fruits) that it's refreshing to hear others are challenged as well by these guys.
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Tweeq
Tweeq of Nature


Registered: 06/07/18
Posts: 2,043
Loc: Netherlands
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Re: Official Copelandia/Panaeolus Thread [Re: 345536345]
#26013583 - 05/26/19 12:19 AM (4 years, 8 months ago) |
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Yes they are a challenge. Love your pitbull attitude Asura, I think that is the way to go with these guys.
Tossed a ton of plates and I lost ALL the rye berries jars that I made with these to stalling with no visible contams. Probably a moisture content thing in hindsight.
Getting better with using LC to inoc sterilized substrate in bags though. Recipe derived from Mary's grows.
Now, getting them to pin and fruit properly is also no picknick / a bitch  Will be trying a thinner layer of substrate this time, temps have moved up a bit here so that should also improve things. First time around the yield was a whopping dry gram from two trays lol but it felt like a success to me anyway bc we have soo much to learn still, even with cubes. Still just getting started / being an enormous noob.
Now, I'll be making 4 trays instead of two with the same amount of sub, maybe put some perlite underneath to have the sub level about level with the tray 'walls'?
We can't cycle things yet with no humidifier or other fancy stuff so it'll be another low tech attempt.
We'll simply keep going with this and inevetably get better..
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Rad Aghast



Registered: 12/07/18
Posts: 133
Loc: Minnesota
Last seen: 4 months, 30 days
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Re: Official Copelandia/Panaeolus Thread [Re: Tweeq]
#26014274 - 05/26/19 12:15 PM (4 years, 8 months ago) |
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Quote:
Tweeq said: Yes they are a challenge. Love your pitbull attitude Asura, I think that is the way to go with these guys.
Tossed a ton of plates and I lost ALL the rye berries jars that I made with these to stalling with no visible contams. Probably a moisture content thing in hindsight.
Now, getting them to pin and fruit properly is also no picknick / a bitch 
My read on the successful growers' advice is that culture growth rate/form and fruiting potential are related - but that the relationship isn't well understood.
For example, I recently gave up on a pan-HI syringe that I worked on since October but never got one fruit out of it. It would always stall somewhere - either on the plate, in the bag, or in the tray. Pans respond really well to disturbance, so I could get it going again in the bag with a good shake, but it would eventually slow down again... then stop completely. When I first started cultivating that spore sample I couldn't get the spores to germinate on agar...so I had to go to LC first then back to agar. Now in hindsight I would never continue cultivating a sample that isn't even willing to germinate on agar. Fucking waste of six months...
What might be helpful is a library of growth types/rates on culture, the cultivation methods used, and the eventual outcome (fruit/no fruit?). Then we could start to develop a variety-specific qualitative model for the relationship between the phenotype on culture and fruiting potential.
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Asura
Cyantist


Registered: 08/01/11
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Loc: Texas
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Re: Official Copelandia/Panaeolus Thread [Re: Rad Aghast]
#26014299 - 05/26/19 12:33 PM (4 years, 8 months ago) |
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Did you really waste six months, though? I would guess that while you didn't have success, it sounds like you still learned from it 
It's like when several of us had bad overlay issues with the jams a while back. Many methods were tried to overcome it. None of them worked. But that's valuable information. It can be used to save a lot of people time and resources.
One thing I have observed is that a good culture is very resilient. I can break up a colonized bag to absolute bits, lay it out in a tray and five days later that tray is fully colonized. It amazes me every time.
My recent failures seem to be culture related. The mycelium seems tired. I don't know what is going on for sure, but it's like it can't make use of the substrate effectively and just drowns in the bag. The bags don't smell bad either. They smell healthy but the smell is not totally overpowering like it is in a good bag.
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Rad Aghast



Registered: 12/07/18
Posts: 133
Loc: Minnesota
Last seen: 4 months, 30 days
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Re: Official Copelandia/Panaeolus Thread [Re: Asura]
#26014306 - 05/26/19 12:37 PM (4 years, 8 months ago) |
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Quote:
Asura said: Did you really waste six months, though? I would guess that while you didn't have success, it sounds like you still learned from it 
Yeah - you're right. But, I learned by failure for six months!
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SWFL
Gettin' Better All The Time


Registered: 07/04/10
Posts: 1,802
Loc: USA
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What is the latest favorite agar recipe specifically for P. Cyan?
-------------------- Listening to anything I say could be detrimental to your health-
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Asura
Cyantist


Registered: 08/01/11
Posts: 5,047
Loc: Texas
Last seen: 11 days, 21 hours
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Re: Agar Recipe? [Re: SWFL]
#26014341 - 05/26/19 01:05 PM (4 years, 8 months ago) |
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MEA works fine. If you want to go all Gordon Ramsey on it, you can, but I like to keep it simple.
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SWFL
Gettin' Better All The Time


Registered: 07/04/10
Posts: 1,802
Loc: USA
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Re: Agar Recipe? [Re: Asura]
#26014356 - 05/26/19 01:13 PM (4 years, 8 months ago) |
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Ok, thanks. When I searched the topic I found mixed info and one post saying that P. Cyans don't like MEA. Other posts about adding some cow shit tea and whatnot.
Is PDA acceptable as well? Or is MEA better?
-------------------- Listening to anything I say could be detrimental to your health-
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Asura
Cyantist


Registered: 08/01/11
Posts: 5,047
Loc: Texas
Last seen: 11 days, 21 hours
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Re: Agar Recipe? [Re: SWFL]
#26014367 - 05/26/19 01:20 PM (4 years, 8 months ago) |
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I have no direct experience with PDA, sorry.
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mullugh
Stranger

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Re: Agar Recipe? [Re: Asura]
#26014379 - 05/26/19 01:26 PM (4 years, 8 months ago) |
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In good news, my trop LC is a success (my first successful LC ever). I have a feeling this is the way forward as I've been exclusively using agar wedges until now.
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Jakeoncid419
Follow me.....



Registered: 06/26/18
Posts: 4,390
Loc: Basel Switzerland
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Re: Agar Recipe? [Re: mullugh] 1
#26015402 - 05/27/19 02:48 AM (4 years, 8 months ago) |
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This wild specimen Of pan cam Indonesia is playing no games! I isolated and transferred (hunting fast moving thin wispy section) about 4 times before moving to grain. Appears I chose wisely.... 16 hours in fruiting conditions &
 The response of pan mycelium to surface evaporation is immediate and incredibly fast!
-------------------- Natural omt/detox online pant cult classes available last Saturday of every month go to buymeacoffee.com/jakeoncid to sign up (1 on 1 consultations also available JOC PAN TEK CORDYCEPS MILITARIS EXOTICS [
Edited by Jakeoncid419 (05/27/19 02:56 AM)
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Jakeoncid419
Follow me.....



Registered: 06/26/18
Posts: 4,390
Loc: Basel Switzerland
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Re: Agar Recipe? [Re: SWFL]
#26015461 - 05/27/19 04:22 AM (4 years, 8 months ago) |
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Quote:
SWFL said: What is the latest favorite agar recipe specifically for P. Cyan?
P.s here is the Ramsey recipe lol 1000ml distilled water (950g) 32g dextrose (sugar) 16 g malt extract 16 g peptone 8g yeast 20 g agar 10 g potato starch
(Optional below) .3 g ammonium citrate .2 g thiamine .2g magnesium phosphate
You can drop the nutes down considerably depending on what your doing (for my low nute recipe I remove the 32g of sugar (usually glucose) drop my other sugars to 10gs. (Each) I can def say I get very few pan stall outs on this recipe instead I’ve been getting some very interesting looking mycelium
-------------------- Natural omt/detox online pant cult classes available last Saturday of every month go to buymeacoffee.com/jakeoncid to sign up (1 on 1 consultations also available JOC PAN TEK CORDYCEPS MILITARIS EXOTICS [
Edited by Jakeoncid419 (05/27/19 04:23 AM)
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J. Jack Flash
stranger than ever.

Registered: 11/20/13
Posts: 1,500
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right. pressing on.
pasteurizing my first manure based substrate right now. dried grass clippings, aged horse manure, verm. will be spawning three jars of oats which reached 100% after recovering from a shake. three other jars did not recover and will be fed to the pile.
don't yet know what i'm spawning to, in terms of vessels. spawn ratio will end up a pretty high, around 1:2. declaring victory at full colonization. fruit considered a bonus. baby steps.
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the j stands for jesus.2020 new years grow along
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Tweeq
Tweeq of Nature


Registered: 06/07/18
Posts: 2,043
Loc: Netherlands
Last seen: 5 hours, 18 minutes
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Good to see you here too Jack. Good luck with your pan grows!
Our next swing at Pans. 5 shallower trays consolidating (something I foolishly forgot about the first try).

The other substrate bag is also colonizing nicely so we won't be short on pan trays. You get the idea. Even if we get only a few fruits from every tray at least we finally get to trip on pans again (after 20 years, sigh).
So that's the goal this time. May not sound as much but it will be a great personal milestone in my mushcult skills development. Learning to cult pans is why I got into the hobby!
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mullugh
Stranger

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Re: baby steps [Re: Tweeq]
#26016290 - 05/27/19 01:25 PM (4 years, 8 months ago) |
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Quote:
Tweeq said: Good to see you here too Jack. Good luck with your pan grows!
Our next swing at Pans. 5 shallower trays consolidating (something I foolishly forgot about the first try).

The other substrate bag is also colonizing nicely so we won't be short on pan trays. You get the idea. Even if we get only a few fruits from every tray at least we finally get to trip on pans again (after 20 years, sigh).
So that's the goal this time. May not sound as much but it will be a great personal milestone in my mushcult skills development. Learning to cult pans is why I got into the hobby!
Awesome, good luck man. I know the feeling of having pans calling after decades without.
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Jakeoncid419
Follow me.....



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Re: baby steps [Re: mullugh]
#26016727 - 05/27/19 05:12 PM (4 years, 8 months ago) |
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Shmore pins popping up day two! Pan cam Indo (Island of Komodo wild print)
-------------------- Natural omt/detox online pant cult classes available last Saturday of every month go to buymeacoffee.com/jakeoncid to sign up (1 on 1 consultations also available JOC PAN TEK CORDYCEPS MILITARIS EXOTICS [
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SWFL
Gettin' Better All The Time


Registered: 07/04/10
Posts: 1,802
Loc: USA
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Has anybody documented a successful outdoor grow? I've searched but haven't found anything.
-------------------- Listening to anything I say could be detrimental to your health-
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L0rdk4in
A noob


Registered: 05/12/14
Posts: 113
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so still struggling to get clean spawn. Is this jar toast?
This oat jar started off looking great but around 90% it started looking really wet. All my other pan goliath did the same. Not sure why. It was from 7th generation agar plate. This is a picture of the agar on its 8th transfer. It is thick but not smooth at all. I have been trying for months to get pans to work :/
Edited by L0rdk4in (05/27/19 07:37 PM)
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345536345
Pan parent!



Registered: 08/13/17
Posts: 268
Last seen: 2 years, 3 months
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Quote:
J. Jack Flash said: right. pressing on.
pasteurizing my first manure based substrate right now. dried grass clippings, aged horse manure, verm. will be spawning three jars of oats which reached 100% after recovering from a shake. three other jars did not recover and will be fed to the pile.
don't yet know what i'm spawning to, in terms of vessels. spawn ratio will end up a pretty high, around 1:2. declaring victory at full colonization. fruit considered a bonus. baby steps.
Jack - careful with the high spawn rate. Read a bit further to confirm but from what I've read pans from grains have tended to do better on a lower spawn rate than say cubes. I've seen 1:4 recommended (if not doing direct inoculation). Something about too much to much nutrients to munch on. May want to confirm.
Edited by 345536345 (05/27/19 07:22 PM)
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