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OfflineHigh Night Expanse
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Re: Official Copelandia/Panaeolus Thread [Re: El Chupacabra]
    #26007700 - 05/22/19 04:10 PM (4 years, 8 months ago)

Quote:

El Chupacabra said:
Is it normal for pan cyans to colonize plates so slowly? I have a few pan cyan T2 plates and they seem to colonize at about half the rate of any of my cube varieties.

I've been using mostly beer agar plates but the standard MEA plates have had about the same results. Temps are always around 72-74ish
Any suggestions?




Im in the tropics right now and we set our AC to those temps anything below 70 and im too cold. pans probably feel the same. Maybe your recipe is off but the temperature is probably contributing to slow growth.

Try med - low 80s see how that works.


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OfflineEl Chupacabra
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Re: Official Copelandia/Panaeolus Thread [Re: High Night Expanse]
    #26007974 - 05/22/19 06:43 PM (4 years, 8 months ago)

Quote:

High Night Expanse said:
Quote:

El Chupacabra said:
Is it normal for pan cyans to colonize plates so slowly? I have a few pan cyan T2 plates and they seem to colonize at about half the rate of any of my cube varieties.

I've been using mostly beer agar plates but the standard MEA plates have had about the same results. Temps are always around 72-74ish
Any suggestions?



Im in the tropics right now and we set our AC to those temps anything below 70 and im too cold. pans probably feel the same. Maybe your recipe is off but the temperature is probably contributing to slow growth.

Try med - low 80s see how that works.




Will do. I'll have to get me some peptone and try that as well and troubleshoot.
I might look into trying my hand at LC maybe too, it always scared me before but im getting pretty comfortable anymore and I already have all the tools for the job too so I might as well give it a crack.
Thanks everyone.


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OfflineHigh Night Expanse
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Registered: 11/07/18
Posts: 330
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Re: Official Copelandia/Panaeolus Thread [Re: El Chupacabra]
    #26008040 - 05/22/19 07:26 PM (4 years, 8 months ago)

Quote:

El Chupacabra said:
Quote:

High Night Expanse said:
Quote:

El Chupacabra said:
Is it normal for pan cyans to colonize plates so slowly? I have a few pan cyan T2 plates and they seem to colonize at about half the rate of any of my cube varieties.

I've been using mostly beer agar plates but the standard MEA plates have had about the same results. Temps are always around 72-74ish
Any suggestions?



Im in the tropics right now and we set our AC to those temps anything below 70 and im too cold. pans probably feel the same. Maybe your recipe is off but the temperature is probably contributing to slow growth.

Try med - low 80s see how that works.




Will do. I'll have to get me some peptone and try that as well and troubleshoot.
I might look into trying my hand at LC maybe too, it always scared me before but im getting pretty comfortable anymore and I already have all the tools for the job too so I might as well give it a crack.
Thanks everyone.




Everyone else knows better about agar recipes ect especially for pouring lots of plates.

That being said what I do is no pour, straight up mashed potatoe flakes, brown sugar and agar.

No fancy stuff.

Eyeball every thing except the aga and add individually to each container depending on the size of the container.

Small jars glass jars, one spoon of mashed potato-sugar water, ~0.2g agar per jar. No precooking. swirled around once after pcing, while cooling, to be homogenous.

Pan spores germ, cubes germ, they both grow well on it. Shitake tissue. Contams haha. Its too simple.


Edited by High Night Expanse (05/22/19 08:43 PM)


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InvisibleCaps McGee
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Re: Official Copelandia/Panaeolus Thread [Re: High Night Expanse]
    #26008076 - 05/22/19 07:47 PM (4 years, 8 months ago)

Agar powder, light malt extract and water... pour them bitches all day! Lol... grain soak is even simpler: grain soak water (diluted with water if necessary) and agar! Lol... the pan aussie ive been playing with seems slow... on agar, and grain... but the jar went bacterial, so may be a dirty culture! :shrug: never worked with it... will post a culture pic for opinions tmrw if that's cool


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OfflineJakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: Caps McGee]
    #26008162 - 05/22/19 08:52 PM (4 years, 8 months ago)

Yeah I mean at first I thought it was genetics but I’ve now run several new wild pan prints and I’m still getting full dish colonization even with ms inn7-10 day I’m actually gonna hit some dishes tomorrow morning I’ll clock a day by day on them unfortunately I do not have any mea only dishes to do a side by side but y’all can compare to your time frames. I’ll do several different pan species/strains (trop bisp cam cyan)


--------------------
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InvisibleCaps McGee
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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
    #26008218 - 05/22/19 09:21 PM (4 years, 8 months ago)

You use peptane or something though right?


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OfflineJakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: Caps McGee]
    #26008229 - 05/22/19 09:36 PM (4 years, 8 months ago)

Well peptone is what I first noticed preformed better with pans and some other exotics and I’ve slowly vis trial and error arrived at this
1000ml distilled water  (950g)
32g dextrose (sugar)
16 g malt extract
16 g peptone
8g yeast
20 g agar
10 g potato starch

(Optional below)
.3 g ammonium citrate
.2 g thiamine
.2g magnesium phosphate

So far everything has loved it including semp which germinated 10/10 dishes in 5 days


--------------------
Natural omt/detox
online  pant cult classes available last Saturday of every month go to buymeacoffee.com/jakeoncid to sign up (1 on 1 consultations also available
JOC PAN TEK
CORDYCEPS MILITARIS

EXOTICS
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InvisibleCaps McGee
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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
    #26008261 - 05/22/19 09:57 PM (4 years, 8 months ago)

:takingnotes:

Very interesting, though it'll likely be some time (if ever) before I get too much more complicated (it's not) than standard MEA lol... though I've been thinking about getting venom to try and cross... does species matter? Lol... I don't even know how one acquires these things


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OfflineJakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: Caps McGee]
    #26008273 - 05/22/19 10:04 PM (4 years, 8 months ago)

Yes type of venom matter very much so and I’m still in the process of determining exactly how effective it is. I am hopeful. Getting vetted by a venom lab was not easy however They will not just send venom to anyone (for obvious reasons). I had to prove I had the equipment to handle it and get a local lab to vouch for me (local water testing lab I know the owner of) If I can prove it definitively viable pre made mycological purpose solution will be made available.


--------------------
Natural omt/detox
online  pant cult classes available last Saturday of every month go to buymeacoffee.com/jakeoncid to sign up (1 on 1 consultations also available
JOC PAN TEK
CORDYCEPS MILITARIS

EXOTICS
[


Edited by Jakeoncid419 (05/22/19 10:05 PM)


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InvisibleCaps McGee
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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
    #26008325 - 05/22/19 10:50 PM (4 years, 8 months ago)

:rockon:
That's what's up...


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OfflineDoc9151M
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Re: Official Copelandia/Panaeolus Thread [Re: Caps McGee] * 1
    #26009178 - 05/23/19 11:20 AM (4 years, 8 months ago)

Jake, I don't use root hormones to get my clones to root, I use cinnamon powder, it works just as good and is much cheaper, the cinnamon also is an antiseptic that keeps the clone safe until roots form.


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Psilocybe cubensis data collection thread. please help with this project if you hunt wild cubensis.
https://www.shroomery.org/forums/showflat.php?Cat=0&Number=26513593&page=0&vc=1#26513593


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OfflineJakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: Doc9151]
    #26009196 - 05/23/19 11:31 AM (4 years, 8 months ago)

Yeah the hormones were not as big as a influencer as the aero missed system just having the hydroponic routing system instead of using soil drastically improved my clone success rate the cloning solution I use clone X is relatively inexpensive for the amount I get and how long it lasts


--------------------
Natural omt/detox
online  pant cult classes available last Saturday of every month go to buymeacoffee.com/jakeoncid to sign up (1 on 1 consultations also available
JOC PAN TEK
CORDYCEPS MILITARIS

EXOTICS
[


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OfflineHigh Night Expanse
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Re: Official Copelandia/Panaeolus Thread [Re: Doc9151]
    #26010006 - 05/23/19 07:03 PM (4 years, 8 months ago)

thanks for the cinnamon tip, quite the coincidence i was just thinking today how well a glove box would work for cloning fruit trees.  If you know any great links itd be much appreciated


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OfflineHigh Night Expanse
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Re: Official Copelandia/Panaeolus Thread [Re: High Night Expanse] * 1
    #26010024 - 05/23/19 07:20 PM (4 years, 8 months ago)




Another straw sub, with leaf litter casing.


Edited by High Night Expanse (10/04/19 10:26 AM)


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OfflineRad Aghast
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Re: Official Copelandia/Panaeolus Thread [Re: High Night Expanse]
    #26010065 - 05/23/19 07:48 PM (4 years, 8 months ago)

Big beautiful fruits!  :thumbup:


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OfflineAsuraS
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Re: Official Copelandia/Panaeolus Thread [Re: Rad Aghast]
    #26010138 - 05/23/19 08:31 PM (4 years, 8 months ago)

Nice!

:raveface:


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OfflineTweeq
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Re: Official Copelandia/Panaeolus Thread [Re: Asura]
    #26010728 - 05/24/19 08:18 AM (4 years, 8 months ago)

Very nice High Night Expanse!

What kind of fruiting chamber do you use for those?
Looking for an 'in between' solution till we got a Jake-style cycled greenhouse


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OfflineSFS96
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Re: Official Copelandia/Panaeolus Thread [Re: Tweeq]
    #26010752 - 05/24/19 08:50 AM (4 years, 8 months ago)

Looks like he used a SGFC


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OfflineTweeq
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Re: Official Copelandia/Panaeolus Thread [Re: SFS96]
    #26010858 - 05/24/19 10:27 AM (4 years, 8 months ago)

I tried that too but some kind of fly or mosquito uses the holes to enter the sgfc and it lays eggs in my sub and then a fuckton of flies comes out of it so maybe I'll modify some of my mini-monos for more fae and maybe set up a little pc fan nearby or something. Idk yet. Making two more pan tubs tonight so I have 5 to 7 days to figure it out :smile:


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InvisibleCaps McGee
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Re: Official Copelandia/Panaeolus Thread [Re: Tweeq]
    #26010888 - 05/24/19 10:58 AM (4 years, 8 months ago)

SGFC with single layer micropore would likely do the trick :shrug:


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