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Caps McGee
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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
#26001607 - 05/19/19 01:22 PM (4 years, 8 months ago) |
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Drooling!
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mary fairchild
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Re: Official Copelandia/Panaeolus Thread [Re: Rad Aghast]
#26001633 - 05/19/19 01:45 PM (4 years, 8 months ago) |
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Quote:
Rad Aghast said: @Mary
Wondering did you iterate on agar for these grows (select for growth on agar)? Or did you go straight from agar (x1) to LC?
I've been curious about the importance of iterating on agar other than for the purpose of ensuring a clean transfer to LC. Intuitively, to me it makes sense to start out with as much diversity as possible to not inadvertently select against fruiting genetics.
Yes, this was four agar selections. The starting prints were taken from wild outdoor grows- often dirty- so I did agar work. The thing is the agar work I do to make sure the mycelia is clean inadvertently selects for narrower genetics than i'd get with multispore (the starting dab of spores).
When I clean a culture on agar I always streak the plate. Its way easier to separate mycelia from contams quickly than just dabbing spores to the middle of a plate and dealing with whatever may be hidden in there. The problem with streaking- at least my take on it- is that its also separates populations. So I was forced to SELECT colonies by cleaning the culture: Here are four selections of Beulah I made, one passage from the streaked plate- arrows show where small wedges went directly to liquid culture:

In the end C1 and C2 fruited 3X to 4X better than A or B. When making selections I would select for disparate forms. In this grow the more wispy forms fruited better. (BTW- I have tried to link growth form on agar to inclination to fruit & I couldn't find any clear relationship).
I think multispore gives you an average of the bell curve of possible "fruiting" outcomes. Selections can give you better or worse outcomes at fruiting. So if you are forced to make selections because you want to make sure its clean then its best to screen several selections. (BTW- the best Pan grow I ever saw was Blue Helixes multispore grow of Jamaica- I think it was a vendor spore syringe directly to LC- go figure).
In hindsight I wish I had done MS as well as the selections- especially since that dab of spores from that outdoor print looked clean to me anyway- to Doc!
Edited by mary fairchild (05/19/19 02:55 PM)
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Asura
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Re: Official Copelandia/Panaeolus Thread [Re: mary fairchild]
#26001861 - 05/19/19 03:43 PM (4 years, 8 months ago) |
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mary, I'm a little confused. You made 4 LC's from the indicated wedges?
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mary fairchild
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Re: Official Copelandia/Panaeolus Thread [Re: Asura]
#26001870 - 05/19/19 03:46 PM (4 years, 8 months ago) |
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Yes, 4 LC's-> 4 trays.
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Rad Aghast



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Re: Official Copelandia/Panaeolus Thread [Re: mary fairchild]
#26002142 - 05/19/19 06:33 PM (4 years, 8 months ago) |
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Quote:
mary fairchild said:
I think multispore gives you an average of the bell curve of possible "fruiting" outcomes. Selections can give you better or worse outcomes at fruiting. So if you are forced to make selections because you want to make sure its clean then its best to screen several selections.
very interesting - this is what I suspected. thx!
How many transfers to get to these three plates? Do the C1 and C2 wedges correspond to the two buelah trays of fruits in the earlier photo? If so - then that is pretty strong evidence for fruiting preference for the beulah growth type in C unless there were covariates.
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Jakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: Rad Aghast]
#26002158 - 05/19/19 06:42 PM (4 years, 8 months ago) |
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Both these are iso’s Pulled from clones Pan Australia red spore & pan hausteca purple spore
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mary fairchild
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Re: Official Copelandia/Panaeolus Thread [Re: Rad Aghast]
#26002426 - 05/19/19 09:11 PM (4 years, 8 months ago) |
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Quote:
Rad Aghast said:
Quote:
mary fairchild said:
I think multispore gives you an average of the bell curve of possible "fruiting" outcomes. Selections can give you better or worse outcomes at fruiting. So if you are forced to make selections because you want to make sure its clean then its best to screen several selections.
very interesting - this is what I suspected. thx!
How many transfers to get to these three plates? Do the C1 and C2 wedges correspond to the two buelah trays of fruits in the earlier photo? If so - then that is pretty strong evidence for fruiting preference for the beulah growth type in C unless there were covariates.
This time it took one transfer from the starting streaked plate. I dont know if I ever went more than 4 transfers. Yes- those are the two in the pic. The "C" plate had at least four sectors to it. I could have probably teased monocultures from them with some more transfers- but since a monoculture doesn't guarantee fruiting capability- I didn't feel like spending the extra time. Since C1 and C2 were not completely separated they may share phenotypes- they did behave practically the same way at fruiting. It may be that a wispy phenotype on agar is tied to better fruiting for Beulah itself (I dont usually do the same grow more than once but it would be interesting to see) but I won't necessarily extend that to other varieties/strains of Pan cyan. I've seen good fruiting from more cottony forms in other varieties of the same species.
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mary fairchild
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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
#26002436 - 05/19/19 09:24 PM (4 years, 8 months ago) |
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Quote:
Jakeoncid419 said: Both these are iso’s Pulled from clones Pan Australia red spore & pan hausteca purple spore

Awesome grows Jake. Great big caps i'll make great big prints! Have you decided to switch more to greenhouse grows now?
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Jakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: mary fairchild]
#26002437 - 05/19/19 09:28 PM (4 years, 8 months ago) |
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Thanks Mary! Yeah I’ve been focusing on isolation of good printing characteristics on all my pans. And yeah it’s all in the tent now. The tubs were kicking out more mushrooms than I could print and eat lol. The small trays are much more manageable.
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Edited by Jakeoncid419 (05/19/19 10:16 PM)
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Jakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419] 1
#26002476 - 05/19/19 10:04 PM (4 years, 8 months ago) |
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Tweeq
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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
#26002532 - 05/19/19 10:57 PM (4 years, 8 months ago) |
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Great grows again, Jake and Mary!
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Adas
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Re: Official Copelandia/Panaeolus Thread [Re: Tweeq]
#26002803 - 05/20/19 04:46 AM (4 years, 8 months ago) |
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Does my Bisporus look like it's about ready to pin? The myc looks SUPER healthy.
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Jakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: Adas]
#26005427 - 05/21/19 12:19 PM (4 years, 8 months ago) |
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Quote:
Adas said: Does my Bisporus look like it's about ready to pin? The myc looks SUPER healthy.

I haven’t bulked my bisp yet but I’d say it looks good I’d increase hydration by 15%
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Adas
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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
#26005491 - 05/21/19 12:44 PM (4 years, 8 months ago) |
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Thanks man. The thing on top is just sand casing so it may look a bit dry. Im waiting for pins now, but from previous attempt it seems that Bisporus may require a solid dunk/heavy misting before it decides to pin.
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El Chupacabra
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Re: Official Copelandia/Panaeolus Thread [Re: Adas]
#26007259 - 05/22/19 11:40 AM (4 years, 8 months ago) |
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Is it normal for pan cyans to colonize plates so slowly? I have a few pan cyan T2 plates and they seem to colonize at about half the rate of any of my cube varieties.
 I've been using mostly beer agar plates but the standard MEA plates have had about the same results. Temps are always around 72-74ish Any suggestions?
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Jakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: El Chupacabra]
#26007286 - 05/22/19 11:55 AM (4 years, 8 months ago) |
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In my experience pans do not like straight MEA. They like peptone the recipe ive found them to like the best took me a while to come up with but has constantly out preformed All my other recipes and is as follows. 1000ml distilled water (950g) 32g dextrose (sugar) 16 g malt extract 16 g peptone 8g yeast 20 g agar 10 g potato starch
(Optional below) .3 g ammonium citrate .2 g thiamine .2g magnesium phosphate
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Asura
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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
#26007336 - 05/22/19 12:32 PM (4 years, 8 months ago) |
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I use MEA with good results. Sometimes I mix it up by making an hpoo tea with the water first.
I prefer the fast plates but I will put a nice looking plate to LC though. If the LC grows out fast I'll use it.
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Jakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: Asura]
#26007391 - 05/22/19 01:08 PM (4 years, 8 months ago) |
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Yeah I’ve definitely gotten them on all recipes but some clearly outperform others across the spectrum especially when it comes to multi spores. I have heard from lots of people that they have trouble with MEA though. You make LC first for most of your inoculations tho don’t you? so you don’t need the entire dish to grow out not saying that they don’t I’m just asking if you do for LC you only need a small wedge so 100% plate colonization is not needed
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Edited by Jakeoncid419 (05/22/19 01:13 PM)
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Doc9151
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Re: Official Copelandia/Panaeolus Thread [Re: Asura]
#26007405 - 05/22/19 01:19 PM (4 years, 8 months ago) |
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Quote:
Asura said: I use MEA with good results. Sometimes I mix it up by making an hpoo tea with the water first.
I prefer the fast plates but I will put a nice looking plate to LC though. If the LC grows out fast I'll use it.
Me too, MEA with a gram or 2 of yeast added to every 25g of agar works well for me
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Jakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: Doc9151]
#26007540 - 05/22/19 02:38 PM (4 years, 8 months ago) |
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So here is what you want ur poo to look like once you have properly prepped it!
Lord of the poop lol look to The Amish and Mennonite’s I’m finding they have excellent aged poo, that they constantly fluff with this big fan like device. Really cool ppl also!

To follow up on the agar yeah I mean I can get them to work with anything but I have had a couple people come to me and more so vendors have told me they have gotten several complaints of ss’s not working. I did a little investigation and I do consistently get higher germination rates with this recipe. That’s not to say other ways will not work it’s the same with the Kratom clones. I got 1 out of 5 using solid and rooting powder and I’ve gotten 4 out of 4 using aero mist system. Personally I think the main problem is The ss’s itself. They can be problematic. I’d much rather work with a print but for some reason about 90% of sales are ss’s (so I’m told)
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Edited by Jakeoncid419 (05/22/19 05:58 PM)
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