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firstTIMER420
Born the son of a sharecropper..



Registered: 05/08/15
Posts: 1,025
Loc: US, maybe?
Last seen: 6 years, 4 days
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Re: Official Copelandia/Panaeolus Thread [Re: cronicr]
#22576550 - 11/26/15 01:21 AM (8 years, 2 months ago) |
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I got my own Pan cyan on the grains, still cleaning up MADS though, it looks really nice on agar, but for some reason its not all fluffy like it was when I first got it on agar, I think it might be the more nutrient heavy mix.
I cant wait to see what goodies I get from you
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Edited by firstTIMER420 (11/26/15 01:21 AM)
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Canaderp
I'm tryin'

Registered: 11/26/15
Posts: 17
Loc: Canada
Last seen: 2 months, 5 days
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Re: Official Copelandia/Panaeolus Thread [Re: firstTIMER420]
#22577515 - 11/26/15 10:01 AM (8 years, 2 months ago) |
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Hello everyone. First post! I'm a long time lurker. I have been growing cubensis for a few years now and have learned everything I know from this site (and a few others!) and it's knowledgable members. I am not a noob to cubensis. My interest in cyans and their cultivation is what has led me to where I am now. I made an account and joined the discussion because I now find myself completely stumped with a problem I just can't figure out. I am trying to grow pan cyan jamaicans from a print I traded with a friend. I made a syringe, made an LC (LC's have been working well for me for years), and have been following waylitjims tek damn near to the letter! Now I find myself with a colonized casing layer and was hoping for fruits only to be plagued by the dreaded mutant blobs of mycelium (which are now bruising blue). Here's a shot of my shitty setup:





I'm using one of my old monos with polyfill and also perlite to try and keep up rh. I've been fanning four or five times a day in addition to the poly. I have a small fan running indirectly and temps are a steadily between 76 and 79. Getting pans to fruit have been on my bucket list for the better part of a decade so yeah, I'm geting depressed. These have been in fruiting for almost ten days and I stopped misting once they colonized the casing layer. Dear god what am I doing wrong?! 
Any advice from you guys would be awesome. Now that my problem has over ridden my social anxiety I hope to more fully be a part of the community aspect of the shroomery... Thanks.
CDP
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Re: Official Copelandia/Panaeolus Thread [Re: Canaderp]
#22577644 - 11/26/15 10:33 AM (8 years, 2 months ago) |
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Welcome to the shroomery and congrats on making the jump to pan cyans, they're definitely worth the extra time and effort. The trip you get from one dried gram of these is unlike any cube trip you'll ever have, the visuals are just off the charts.
Now with your setup, what is your substrate composed of and why did you case with vermiculite, that'll dry up quickly if not misted regularly. And even then, misting prior to pin formation could cause lots of aborts. Pan mycelium is a lot more sensitive than cubes, so it's best to avoid it.
Pans prefer a pasteurized peat moss/verm/lime casing (or "Jiffy mix", which is essentially the same thing). Once you apply the casing, just put it in your fruiting chamber (no misting) and wait for fruits to pop up. After you pick the first flush, then you can mist it.
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firstTIMER420
Born the son of a sharecropper..



Registered: 05/08/15
Posts: 1,025
Loc: US, maybe?
Last seen: 6 years, 4 days
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Re: Official Copelandia/Panaeolus Thread [Re: Psilosoulful]
#22578535 - 11/26/15 02:07 PM (8 years, 2 months ago) |
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I thought you were supposed to mist pans until pins form, then stop.
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Re: Official Copelandia/Panaeolus Thread [Re: firstTIMER420]
#22578589 - 11/26/15 02:18 PM (8 years, 2 months ago) |
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Once I see primordia develop, that's when i slow down on the misting. Maybe once per day. Misting regularly up until the pins start to form will weaken them and they might abort. Some people can get away with it, but I like to be on the safe side.
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Canaderp
I'm tryin'

Registered: 11/26/15
Posts: 17
Loc: Canada
Last seen: 2 months, 5 days
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Re: Official Copelandia/Panaeolus Thread [Re: Psilosoulful]
#22578759 - 11/26/15 03:02 PM (8 years, 2 months ago) |
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Thank you Psilosoulful for the welcome! I am interested in the effects of the pans. No more choking down 5+ g. of cubes. Blech. I have read everything I can about them and hope to experience them soon (hence my frustration!). OK, I used equal parts field cured hpoo (which the mycelium rips thru in a couple days!), brf, and verm. Loaded into the ziploc pp5 cups and inoculated with my trusty LC. I did use vermiculite as a casing layer because it's all I had and didn't think it would matter... Thank you for the jiffy mix advice. I will proceed to my nearest garden center and grab a bag. I'm trying to keep that sweet spot between high rh and lots of fae but you're right, that verm layer just keeps drying out. At the beginning, in my zeal, I think I did mist too much. A common mistake, I know. Can these be saved? Are the blobs mutant fruits? I picked some off and they bruised a very deep dark blue...
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firstTIMER420
Born the son of a sharecropper..



Registered: 05/08/15
Posts: 1,025
Loc: US, maybe?
Last seen: 6 years, 4 days
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Re: Official Copelandia/Panaeolus Thread [Re: Canaderp]
#22579056 - 11/26/15 04:34 PM (8 years, 2 months ago) |
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My oans are colonizing slow in the jars. I think it might be due to the 76f temp in my house.
Any ideas on sources of slight heat for pans? From reading, they should be exploding on the grains.
Another thing might be my grains too wet, but I always do the same procedure and actually let them sit out longer and get a little not drier before pcing them, cubes are always fine with my method.
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Re: Official Copelandia/Panaeolus Thread [Re: Canaderp]
#22580251 - 11/26/15 10:27 PM (8 years, 2 months ago) |
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Quote:
Canaderp said: Thank you Psilosoulful for the welcome! I am interested in the effects of the pans. No more choking down 5+ g. of cubes. Blech. I have read everything I can about them and hope to experience them soon (hence my frustration!).

Quote:
Canaderp said: Can these be saved? Are the blobs mutant fruits? I picked some off and they bruised a very deep dark blue...
Don't pick them off, they should continue to colonize the verm. Just give it a light misting and wait for the first signs of primordia on the surface, then slow it down to once a day (and mist from a distance, not directly) Like I mentioned before, too much misting at this stage might cause them to abort.
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Re: Official Copelandia/Panaeolus Thread [Re: firstTIMER420]
#22580261 - 11/26/15 10:30 PM (8 years, 2 months ago) |
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Quote:
firstTIMER420 said: My oans are colonizing slow in the jars. I think it might be due to the 76f temp in my house.
Any ideas on sources of slight heat for pans? From reading, they should be exploding on the grains.
Yes, pans are a tropical species, so they should grow faster in those temps.
Quote:
firstTIMER420 said: Another thing might be my grains too wet, but I always do the same procedure and actually let them sit out longer and get a little not drier before pcing them, cubes are always fine with my method.
As long as you have a good GE filter, your grains should evaporate off the excess moisture. And shake them several times a day to distribute the moisture throughout the jar.
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firstTIMER420
Born the son of a sharecropper..



Registered: 05/08/15
Posts: 1,025
Loc: US, maybe?
Last seen: 6 years, 4 days
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Re: Official Copelandia/Panaeolus Thread [Re: Psilosoulful]
#22580355 - 11/26/15 11:01 PM (8 years, 2 months ago) |
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Im thinking I may have made my GE hole too small, byt like I said it works fine for everything else. I use like 1/4 inch I think.
I thought pans were supposed to be in the 78F range. The past coule nights have had the only cold weather weve gotten all year, of course right when I inoculate some pans.
How long does your pans usually take to take off on grains?(from agar)
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Re: Official Copelandia/Panaeolus Thread [Re: firstTIMER420]
#22580381 - 11/26/15 11:09 PM (8 years, 2 months ago) |
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A 1/4 inch will work fine. What do you have covering it? I just stuff them tightly with polyfill, I got tired of using SFD's. Works great for me so far.
Don't worry about the temp too much, they'll still colonize, just a tad slower in the lower temp ranges (but the stems will be denser, so that's a plus)
My agar wedges usually spread to the grains within a week. The following week it picks up speed and when I see it's about 30% colonized I'll give it a good shake and wait for it to finish up.
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firstTIMER420
Born the son of a sharecropper..



Registered: 05/08/15
Posts: 1,025
Loc: US, maybe?
Last seen: 6 years, 4 days
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Re: Official Copelandia/Panaeolus Thread [Re: Psilosoulful]
#22580420 - 11/26/15 11:22 PM (8 years, 2 months ago) |
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gotcha, its been abou 4 days and im barely seeing any growth.
Ill update soon. I think I shook them too hard. and im going to use half plate in each quart next time, instead of a quarter.
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Re: Official Copelandia/Panaeolus Thread [Re: firstTIMER420]
#22580440 - 11/26/15 11:31 PM (8 years, 2 months ago) |
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Yeah, that'll definitely speed things up, more inoculation points for the grains 
Last time, I just used a tiny wedge, i was trying to conserve my plates. 
IMO making a LI is the most efficient route to take. You can knock up a bunch of jars easily with just 1-2cc's of solution, and they'll colonize soo much quicker than agar wedges ever will.
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firstTIMER420
Born the son of a sharecropper..



Registered: 05/08/15
Posts: 1,025
Loc: US, maybe?
Last seen: 6 years, 4 days
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Re: Official Copelandia/Panaeolus Thread [Re: Psilosoulful]
#22580471 - 11/26/15 11:51 PM (8 years, 2 months ago) |
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Yea, I really want to start using LI's, but I have 6 jars of LC I made 2 months ago I have yet to use.
Im really hoping these colonize, im really excited to grow some pans. Either way, I have MADS spores on agar, just transferred a second time, narrowing down genetics, and I finally made my slants(pain in the ass in the SAB ill tell you what)
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Canaderp
I'm tryin'


Registered: 11/26/15
Posts: 17
Loc: Canada
Last seen: 2 months, 5 days
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Re: Official Copelandia/Panaeolus Thread [Re: firstTIMER420]
#22581221 - 11/27/15 08:08 AM (8 years, 2 months ago) |
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OK so last night I checked the tub and there was a bunch of pins! Woo hoo. But wait, they all seem to be aborting Boo hoo! This morning they hadn't really grown any bigger and were getting a kind of bluish tinge to them. Damn. I've been leaving the top of the bin open a crack to promote better fae over night. I know when I grow cubes they nearly double in size at this stage in the span of ten hours so I wonder where my weakest link in the chain is... Anyway, after this flush (if I get one) I'm going to recase with the jiffy mix. I'll try and snap a pic of the shitty pins in a couple hours but my phone is a potato so hopefully I can get a decent shot. Thank you Psilosoulful for your help and advice! I really appreciate it!
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ukshroomer
Law Abider


Registered: 11/08/09
Posts: 484
Loc: Netherlands
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Re: Official Copelandia/Panaeolus Thread [Re: Canaderp]
#22581408 - 11/27/15 09:17 AM (8 years, 2 months ago) |
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Hello,
I inoculated some panaeolus rye jars about 7 days ago. I have not seen any sign signs of germination yet. I also inoculated some mexican cube jars 5 days ago and they are all showing signs of growth. The temps are around 78. How long does it usually take you to see signs of growth with pans. The strain is cyanescens goliath. Should I order new spores if I don't see signs soon? Please let me know. I want this project to work
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Canaderp
I'm tryin'


Registered: 11/26/15
Posts: 17
Loc: Canada
Last seen: 2 months, 5 days
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Re: Official Copelandia/Panaeolus Thread [Re: ukshroomer]
#22581429 - 11/27/15 09:23 AM (8 years, 2 months ago) |
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Hey ukshroomer! I'm not nearly an expert with pans but when I inoculated my LC's they took over a week to show any signs of life. I also hit up a grain jar with spores (to see if I was introducing any contaminants to my LC's) and it also was slow to start, but once it took off it really took off...
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Canaderp
I'm tryin'


Registered: 11/26/15
Posts: 17
Loc: Canada
Last seen: 2 months, 5 days
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Re: Official Copelandia/Panaeolus Thread [Re: Canaderp]
#22581450 - 11/27/15 09:28 AM (8 years, 2 months ago) |
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OK, here's the potato pin pics. Hard to see the bluish (some even greyish) tinge but it's there. Anyone know what I should be doing to promote pin survival here? I'm starting to believe I'm overthinking it all and I should just stfu and leave them alone... 



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ukshroomer
Law Abider


Registered: 11/08/09
Posts: 484
Loc: Netherlands
Last seen: 13 days, 27 minutes
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Re: Official Copelandia/Panaeolus Thread [Re: Canaderp]
#22581489 - 11/27/15 09:37 AM (8 years, 2 months ago) |
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thanks for the response. i feel better knowing that I am not alone in getting slow germination. i am just hoping i didnt get bunk spores.
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Canaderp
I'm tryin'


Registered: 11/26/15
Posts: 17
Loc: Canada
Last seen: 2 months, 5 days
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Re: Official Copelandia/Panaeolus Thread [Re: ukshroomer]
#22581519 - 11/27/15 09:44 AM (8 years, 2 months ago) |
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I hope you didn't get bunk spores either... I ordered pan spore syringes three different times over the past five years. Every. Single. Syringe. Nothing... I finally made contact with a guy in Germany through reddit and he sent me some prints. I made my own syringe and that's how I got here.
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