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nube424


Registered: 12/03/17
Posts: 6,063
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Re: Official Copelandia/Panaeolus Thread [Re: rickyswamps]
#25825717 - 02/20/19 05:21 PM (4 years, 11 months ago) |
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Thin casing. High temps. Little bit more fae than cubes. And good luck!
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Jakeoncid419
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Registered: 06/26/18
Posts: 4,390
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Re: Official Copelandia/Panaeolus Thread [Re: rickyswamps]
#25825719 - 02/20/19 05:22 PM (4 years, 11 months ago) |
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Quote:
rickyswamps said: Speaking of Pan Jams….
I'm going to case this tonight:

I have let it sit colonized for a few days, part because of suggestions I have heard and part because I didn't have time to pasteurize casing. Its forming aerial blobs which are even higher than a 1/4" casing layer I want to put down. Anyone have any words of advice on getting this to work out…
It is 50/50 cow manure compost and coir. Spawned to bulk from grain.
Properly pasteurize ur case don’t go over 170f if you let it sit a few more days ur microbe count it will help but not needed most importantly keep ur casing THIN!!!!! As thin as you can and still call it a case. U just need a lil but to act as the buffer between evap and rehydration
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Asura
Cyantist


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Re: Official Copelandia/Panaeolus Thread [Re: mary fairchild]
#25825775 - 02/20/19 05:40 PM (4 years, 11 months ago) |
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Quote:
mary fairchild said:
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Asura said:
Quote:
345536345 said:
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mary fairchild said: I like the case to be ALMOST saturated with water up to and during pinning- meaning just get the case to the point where it starts to glisten with water either with a syringe or a sprayer, then DONT add any more water, thats plenty. In a minute that water should spread thru the case and no longer glisten- but just look damp. The way I see it- that will be enough water to saturate the case down to the substrate level. If the case always looks like its glistening then its too wet. If you have slow growth anyway then the problem is not moisture level of the case.
Mary - for this I have some small water "pools" resting on top of my Cambo tray currently consolidating and waiting to be put into fruiting. I drained off the top by angling the tray but think dabbing with paper towels is probably more efficient and less risky. Is that what you've typically done? No lift-off of the myc when doing so?
All my trays usually have these pools to various degrees. I just put the casing on top. If you cover the trays with foil and poke a few holes in the top (like RR does) you will have less pooling. I've started doing this lately and my trays are coming out fine.
Same here- If there's a lot of water/metabolites, I sop em up with a paper towel. Otherwise when you lay the case it should absorb it.
I used to think it was metabolites but I'm not so sure. Was thinking it might just be poop water
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Rad Aghast



Registered: 12/07/18
Posts: 133
Loc: Minnesota
Last seen: 4 months, 29 days
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Re: Official Copelandia/Panaeolus Thread [Re: Asura]
#25826103 - 02/20/19 07:32 PM (4 years, 11 months ago) |
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Curious if anyone has ever just tried a poo/verm sub with success.
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nube424


Registered: 12/03/17
Posts: 6,063
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Re: Official Copelandia/Panaeolus Thread [Re: Rad Aghast]
#25826123 - 02/20/19 07:43 PM (4 years, 11 months ago) |
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I did poo and coir. Pretty much the same thong. I don't see why that wouldn't work if that's what u have?
Anything can work of u get conditions and genetics right.
And I agree with the poop water asura. Water leaches color from the substrate. If the liquid is "thick" or viscous, that might be metabolites. But yellow water is yellow water. Imo.
Edited by nube424 (02/20/19 07:45 PM)
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hazelnut
Mr



Registered: 06/14/13
Posts: 157
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Re: Official Copelandia/Panaeolus Thread [Re: Asura]
#25827163 - 02/21/19 08:47 AM (4 years, 11 months ago) |
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Quote:
mary fairchild said: I like the case to be ALMOST saturated with water up to and during pinning- meaning just get the case to the point where it starts to glisten with water either with a syringe or a sprayer, then DONT add any more water, thats plenty. In a minute that water should spread thru the case and no longer glisten- but just look damp. The way I see it- that will be enough water to saturate the case down to the substrate level. If the case always looks like its glistening then its too wet. If you have slow growth anyway then the problem is not moisture level of the case.
Late to see this, but thank you for this advice! Pasting into my notepad 
Here's an update on my grow. The fruits are growing slowly, but my temperatures have been dropping to low 70s, which could be a factor in this. I watered the casing with a syringe, following Mary's advice above.
I replaced the lids with dub tubs because some fruits were hitting the ceiling! I taped over the dubtub holes because I realized they were useless in terms of increasing FAE to the substrate, but they could have been causing excessive drying of the substrate/casing.
Something I noticed: the two tubs that are at a 1:2 spawn to substrate ratio are performing much better than the two 1:1 ratio tubs (see below). I can start to smell bacteria in one tub, and can see yellow on the sides of the substrate. This leads me wonder, perhaps the small fruit sizes are due to competition with bacteria? And the tubs with a lower spawn-sub ratio (1:2) are performing better than a higher spawn-sub ratio (1:1), because they have a higher proportion of "good" bacteria – found in the hpoo – which is protecting the mushroom culture from "bad" bacteria – coming from spawn that is likely not super clean
Thoughts?
Next time I might try this same pan aussie culture with a 1:3 or 1:4 spawn to substrate ratio
TUB D – 1:2 spawn:sub ratio
 
TUB A – 1:2 spawn:sub ratio (note the fruit that hit the lid, lol)
 
TUB C – 1:1 spawn:sub ratio
 
TUB C – 1:1 spawn:sub ratio

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Jakeoncid419
Follow me.....



Registered: 06/26/18
Posts: 4,390
Loc: Basel Switzerland
Last seen: 1 day, 12 hours
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Re: Official Copelandia/Panaeolus Thread [Re: hazelnut]
#25827407 - 02/21/19 10:21 AM (4 years, 11 months ago) |
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Quote:
hazelnut said:
Quote:
mary fairchild said: I like the case to be ALMOST saturated with water up to and during pinning- meaning just get the case to the point where it starts to glisten with water either with a syringe or a sprayer, then DONT add any more water, thats plenty. In a minute that water should spread thru the case and no longer glisten- but just look damp. The way I see it- that will be enough water to saturate the case down to the substrate level. If the case always looks like its glistening then its too wet. If you have slow growth anyway then the problem is not moisture level of the case.
Late to see this, but thank you for this advice! Pasting into my notepad 
Here's an update on my grow. The fruits are growing slowly, but my temperatures have been dropping to low 70s, which could be a factor in this. I watered the casing with a syringe, following Mary's advice above.
I replaced the lids with dub tubs because some fruits were hitting the ceiling! I taped over the dubtub holes because I realized they were useless in terms of increasing FAE to the substrate, but they could have been causing excessive drying of the substrate/casing.
Something I noticed: the two tubs that are at a 1:2 spawn to substrate ratio are performing much better than the two 1:1 ratio tubs (see below). I can start to smell bacteria in one tub, and can see yellow on the sides of the substrate. This leads me wonder, perhaps the small fruit sizes are due to competition with bacteria? And the tubs with a lower spawn-sub ratio (1:2) are performing better than a higher spawn-sub ratio (1:1), because they have a higher proportion of "good" bacteria – found in the hpoo – which is protecting the mushroom culture from "bad" bacteria – coming from spawn that is likely not super clean
Thoughts?
Next time I might try this same pan aussie culture with a 1:3 or 1:4 spawn to substrate ratio
TUB D – 1:2 spawn:sub ratio
 
TUB A – 1:2 spawn:sub ratio (note the fruit that hit the lid, lol)
 
TUB C – 1:1 spawn:sub ratio
 
TUB C – 1:1 spawn:sub ratio
 
Congrats bruh!! Yes I’ve found 1:4 works best, also genetics are a huge factor clone your best fruits, and start hunting iso’s. Well done!
Here is a 1:4 tray just starting to send up it’s first flush
Edited by Jakeoncid419 (02/21/19 10:50 AM)
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JanSalmon
Stranger
Registered: 01/14/19
Posts: 47
Last seen: 2 years, 11 months
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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
#25828322 - 02/21/19 05:15 PM (4 years, 11 months ago) |
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3 different pans about ready for G2G, looking pretty healthy and happy 

kenya gen4 transfers next to amazon pes cubes.
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Asura
Cyantist


Registered: 08/01/11
Posts: 5,047
Loc: Texas
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Re: Official Copelandia/Panaeolus Thread [Re: JanSalmon]
#25828512 - 02/21/19 06:31 PM (4 years, 11 months ago) |
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I gotta say that I am excited. I've never seen so many people trying pans all at once.
Looking good, Jan. So you have some pans from Kenya? You know the story on those?
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Puduwoke
mushroom enthusiastic


Registered: 06/25/17
Posts: 1,666
Last seen: 2 days, 1 hour
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Re: Official Copelandia/Panaeolus Thread [Re: Asura]
#25828543 - 02/21/19 06:50 PM (4 years, 11 months ago) |
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Well I gotta say, that a big part of it is because this thread AND more importantly people like you, Mary fairchild and Jakeonacid being so willing to help us new pan cultivators out and sharing your experiences! 
Much love too you all!
I have prints coming my way and cannot wait to start trying to cultivate these lovely mushrooms!
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JanSalmon
Stranger
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Re: Official Copelandia/Panaeolus Thread [Re: Asura]
#25828565 - 02/21/19 07:01 PM (4 years, 11 months ago) |
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Quote:
Asura said: I gotta say that I am excited. I've never seen so many people trying pans all at once.
Looking good, Jan. So you have some pans from Kenya? You know the story on those?
I do not actually, care to share?
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Caps McGee
Grandaddy Smurfshack



Registered: 10/28/17
Posts: 14,357
Loc: ally known as ...
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Re: Official Copelandia/Panaeolus Thread [Re: JanSalmon]
#25828593 - 02/21/19 07:12 PM (4 years, 11 months ago) |
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 (?Jan? Idk lol) 
Taking my time with the Aussie, but it's clean, and waiting for an opening in my focus lol
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rickyswamps
Bad Apple



Registered: 11/08/18
Posts: 1,192
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Re: Official Copelandia/Panaeolus Thread [Re: Puduwoke]
#25828631 - 02/21/19 07:38 PM (4 years, 11 months ago) |
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Quote:
Puduwoke said: Well I gotta say, that a big part of it is because this thread AND more importantly people like you, Mary fairchild and Jakeonacid being so willing to help us new pan cultivators out and sharing your experiences! 
Much love too you all!
I have prints coming my way and cannot wait to start trying to cultivate these lovely mushrooms!


Thanks Asura! I waited too long to check these, they are flying off the plate. Tomorrow I'll get them transferred. Behind is my recently cased Pan Jam shoebox from vendor syringe. I am dubtubing it, cracked a little extra, maybe something will come out of it.
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mary fairchild
Pantheist


Registered: 12/06/14
Posts: 777
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Re: Official Copelandia/Panaeolus Thread [Re: rickyswamps]
#25828696 - 02/21/19 08:06 PM (4 years, 11 months ago) |
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Good to see people getting Pans from diverse locations for a change, the selection has otherwise been limited!
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mary fairchild
Pantheist


Registered: 12/06/14
Posts: 777
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Re: Official Copelandia/Panaeolus Thread [Re: hazelnut]
#25828881 - 02/21/19 09:27 PM (4 years, 11 months ago) |
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Quote:
hazelnut said:
Quote:
mary fairchild said: I like the case to be ALMOST saturated with water up to and during pinning- meaning just get the case to the point where it starts to glisten with water either with a syringe or a sprayer, then DONT add any more water, thats plenty. In a minute that water should spread thru the case and no longer glisten- but just look damp. The way I see it- that will be enough water to saturate the case down to the substrate level. If the case always looks like its glistening then its too wet. If you have slow growth anyway then the problem is not moisture level of the case.
Late to see this, but thank you for this advice! Pasting into my notepad 
Here's an update on my grow. The fruits are growing slowly, but my temperatures have been dropping to low 70s, which could be a factor in this. I watered the casing with a syringe, following Mary's advice above.
I replaced the lids with dub tubs because some fruits were hitting the ceiling! I taped over the dubtub holes because I realized they were useless in terms of increasing FAE to the substrate, but they could have been causing excessive drying of the substrate/casing.
Something I noticed: the two tubs that are at a 1:2 spawn to substrate ratio are performing much better than the two 1:1 ratio tubs (see below). I can start to smell bacteria in one tub, and can see yellow on the sides of the substrate. This leads me wonder, perhaps the small fruit sizes are due to competition with bacteria? And the tubs with a lower spawn-sub ratio (1:2) are performing better than a higher spawn-sub ratio (1:1), because they have a higher proportion of "good" bacteria – found in the hpoo – which is protecting the mushroom culture from "bad" bacteria – coming from spawn that is likely not super clean
Thoughts?
Next time I might try this same pan aussie culture with a 1:3 or 1:4 spawn to substrate ratio
TUB D – 1:2 spawn:sub ratio
 
TUB A – 1:2 spawn:sub ratio (note the fruit that hit the lid, lol)
 
TUB C – 1:1 spawn:sub ratio
 
TUB C – 1:1 spawn:sub ratio
 
So theres the argument that high spawn to sub ratios- ie. 1:1 to 1:2, gets you to saturation of the substrate sooner & faster to fruiting before contaminants- other microbes- can take over. Thats what works well for cubes but who's to say thats best Pans?
In "Spawn to Bulk" grows I found a 1:4 to colonize the sub just as fast & work at least as well as a 1:2 (volume grain to volume of substrate (v/v)). If anything i'd play with stretching it out even more. At a 1:4 you can double the surface area available for fruiting vs a 1:2. Our "direct inoculation" grows, done w/w, translate into grain to substrate v/v ratios of somewhere between 1:8 to 1:10.
Edited by mary fairchild (02/22/19 01:29 AM)
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Asura
Cyantist


Registered: 08/01/11
Posts: 5,047
Loc: Texas
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Re: Official Copelandia/Panaeolus Thread [Re: mary fairchild]
#25828901 - 02/21/19 09:41 PM (4 years, 11 months ago) |
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I can tell you what that kinda looks like for my typical grows. 6 quarts of my sub has 250g of milo in it. Seems like almost nothing when it's all mixed up. But it works great.
To clarify to those that don't know, direct inoculation is when you inoculate your substrate (sterilized) in a spawn bag with LC. You grow that out, break it up to a tray, let that recover for 5-10 days or so (for pans) and fruit.
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Jakeoncid419
Follow me.....



Registered: 06/26/18
Posts: 4,390
Loc: Basel Switzerland
Last seen: 1 day, 12 hours
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Re: Official Copelandia/Panaeolus Thread [Re: Asura]
#25828923 - 02/21/19 09:56 PM (4 years, 11 months ago) |
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Quote:
Asura said: I can tell you what that kinda looks like for my typical grows. 6 quarts of my sub has 250g of milo in it. Seems like almost nothing when it's all mixed up. But it works great.
To clarify to those that don't know, direct inoculation is when you inoculate your substrate (sterilized) in a spawn bag with LC. You grow that out, break it up to a tray, let that recover for 5-10 days or so (for pans) and fruit.
U can also use a wedge to direct inoculate (I prefer them to LC unless I’m really going for bulk)
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Tweeq
Tweeq of Nature


Registered: 06/07/18
Posts: 2,043
Loc: Netherlands
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Re: Official Copelandia/Panaeolus Thread [Re: Puduwoke]
#25829049 - 02/21/19 11:24 PM (4 years, 11 months ago) |
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Quote:
Puduwoke said: I have prints coming my way and cannot wait to start trying to cultivate these lovely mushrooms!
Sending you some over the weekend!
Finally got some mycelium growth in my clone dishes. They looked totally bacterial in the beginning. Will transfer this weekend. Also put some of those Ogre Boy spores to agar. (First time for me)
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345536345
Pan parent!



Registered: 08/13/17
Posts: 268
Last seen: 2 years, 3 months
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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
#25829465 - 02/22/19 07:49 AM (4 years, 11 months ago) |
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Any suggestions on adjusting pH of a casing already laid? I didn't test the pH of the casing beforehand assuming it would be somewhat decent but it turns out it's in the high 5's / low 6's. Yeah.... This is after calibrating my meter and double checking with some strips. Anyway....
I'm trying to figure out if I should scrape off the casing of the existing trays (currently 10-15% poking through the casing) or dribble / spray some baking soda mixed with water over the top. Thoughts?
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Puduwoke
mushroom enthusiastic


Registered: 06/25/17
Posts: 1,666
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Re: Official Copelandia/Panaeolus Thread [Re: 345536345]
#25829483 - 02/22/19 08:09 AM (4 years, 11 months ago) |
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I have not a lot of experince. But I would leave it and do not mess with it so much.
I read some people that did not even use anything to change to PH because pans colonize so fast.
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