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Invisiblenube424
Male

Registered: 12/03/17
Posts: 6,063
Re: Official Copelandia/Panaeolus Thread [Re: Caps McGee]
    #25794330 - 02/06/19 06:10 AM (4 years, 11 months ago)

Why do people make life so hard :oldman:


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InvisibleCaps McGee
Grandaddy Smurfshack
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Registered: 10/28/17
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Re: Official Copelandia/Panaeolus Thread [Re: nube424]
    #25794340 - 02/06/19 06:15 AM (4 years, 11 months ago)

Idk, but I've been guilty more than once myself: in many aspects of mine! Lol


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Invisiblenube424
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Re: Official Copelandia/Panaeolus Thread [Re: Caps McGee]
    #25794354 - 02/06/19 06:23 AM (4 years, 11 months ago)

Yea I guess we all do it till we learn the ways. I havnt had good luck with pan lcs. I can make an lc with cubes, but for some reason my pan lcs never grow at all.


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InvisibleCaps McGee
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Re: Official Copelandia/Panaeolus Thread [Re: nube424]
    #25794372 - 02/06/19 06:34 AM (4 years, 11 months ago)

I steer clear of liquids in general and opt to pour my agar super soft (3.5g agar powder and 4g LME to ~525ml of water)... this basically liquefies on the initial shake and serves as an LI/LC essentially: maintaining the speed they offer, while possessing the benefit of easy contamination identification on a 2D surface during colonization


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OfflineRad Aghast
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Re: Official Copelandia/Panaeolus Thread [Re: FunnyFungiName]
    #25794792 - 02/06/19 10:29 AM (4 years, 11 months ago)

Quote:

FunnyFungiName said:
Anyone having trouble with germination try making a lc then putting that back to agar successfully? I was thinking make a few small LC jars and if they colonize then use an inoculation loop to dip in the jars and spread on some agar.




Yes. And I wouldn't be dissuaded by others from taking this approach. Its a logical approach if, as you've already suggested, you can't get your strain to germinate on agar. Difficulty with germination of pans on agar isn't that uncommon. If you scroll back through the archives of this thread you'll see it mentioned more than a couple times.

I was unable to get a vendor purchased syringe of Hawaiians to germinate on MEA. Went to LC instead, had a jar full of liquid mycelium in 8 days. Back to MEA -> golden. I wasn't interested in tinkering with agar recipes - or waiting up to 30 days (as was also suggested at the time). It was the right thing to do.

Aversion to LC seems to originate with stories of folks using contaminated LC in large batches (not testing on agar first) and throwing out a lot of work. If you're going back to agar, as you've already stated, there's no additional risk. You're just mitigating the difficulty with germination.

Once you've got your LC, read up on sterile technique in going from your LC jar to agar. I wouldn't personally ever open an LC jar.


Edited by Rad Aghast (02/06/19 11:34 AM)


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Offlinehazelnut
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Re: Official Copelandia/Panaeolus Thread [Re: Rad Aghast]
    #25794920 - 02/06/19 11:39 AM (4 years, 11 months ago)

is a 1:1 spawn:substrate ratio too high for pan cyan aussie?
I have 4 spawn jars, and only 5 substrate jars


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OfflineJakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: hazelnut]
    #25794983 - 02/06/19 12:03 PM (4 years, 11 months ago)

Quote:

hazelnut said:
is a 1:1 spawn:substrate ratio too high for pan cyan aussie?
I have 4 spawn jars, and only 5 substrate jars



Oh yeah i do 1:4


--------------------
Natural omt/detox
online  pant cult classes available last Saturday of every month go to buymeacoffee.com/jakeoncid to sign up (1 on 1 consultations also available
JOC PAN TEK
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Offlinehazelnut
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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
    #25795054 - 02/06/19 12:36 PM (4 years, 11 months ago)

Quote:

Jakeoncid419 said:
Quote:

hazelnut said:
is a 1:1 spawn:substrate ratio too high for pan cyan aussie?
I have 4 spawn jars, and only 5 substrate jars



Oh yeah i do 1:4




Will they be ok though? Is loss of moisture the main risk with such high ratios? I ended up making 3 trays at 1:1, 2 trays at 1:2. I could make some more substrate to decrease the ratio, but I'm lazy, lol


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Offlinepsilly the kid
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Re: Official Copelandia/Panaeolus Thread [Re: hazelnut]
    #25795069 - 02/06/19 12:41 PM (4 years, 11 months ago)

:threadmonitor:


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pebbles suck
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Offlineshroomms
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Registered: 07/27/14
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Re: Official Copelandia/Panaeolus Thread *DELETED* [Re: Jakeoncid419]
    #25795080 - 02/06/19 12:44 PM (4 years, 11 months ago)

Post deleted by shroomms

Reason for deletion: I wanted to! Thank-you!


Edited by shroomms (02/06/19 12:51 PM)


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OfflineFunnyFungiName
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Registered: 01/15/18
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Re: Official Copelandia/Panaeolus Thread [Re: nube424]
    #25795111 - 02/06/19 12:59 PM (4 years, 11 months ago)

Quote:

nube424 said:
Why not just start it on agar and transfer that to lc?



Apologies for not being clear, but I've been trying to start spores on agar for months with zero luck. Im asking for other alternatives besides buying a new spore syringe. I have used mea, brf, and pda all with no luck. Nothing grows. The vendor is a shroomery sponsor. The variety is sandoze.


Quote:

Rad Aghast said:
Quote:

FunnyFungiName said:
Anyone having trouble with germination try making a lc then putting that back to agar successfully? I was thinking make a few small LC jars and if they colonize then use an inoculation loop to dip in the jars and spread on some agar.




Yes. And I wouldn't be dissuaded by others from taking this approach. Its a logical approach if, as you've already suggested, you can't get your strain to germinate on agar. Difficulty with germination of pans on agar isn't that uncommon. If you scroll back through the archives of this thread you'll see it mentioned more than a couple times.

I was unable to get a vendor purchased syringe of Hawaiians to germinate on MEA. Went to LC instead, had a jar full of liquid mycelium in 8 days. Back to MEA -> golden. I wasn't interested in tinkering with agar recipes - or waiting up to 30 days (as was also suggested at the time). It was the right thing to do.

Aversion to LC seems to originate with stories of folks using contaminated LC in large batches (not testing on agar first) and throwing out a lot of work. If you're going back to agar, as you've already stated, there's no additional risk. You're just mitigating the difficulty with germination.

Once you've got your LC, read up on sterile technique in going from your LC jar to agar. I wouldn't personally ever open an LC jar.



Thank you, I'm going to give it a shot!


Edited by FunnyFungiName (02/06/19 01:04 PM)


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OfflineJakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: shroomms]
    #25795140 - 02/06/19 01:14 PM (4 years, 11 months ago)

Quote:

shroomms said:
Every morning i'm harvesting another flush of these parasol mushrooms...harvested about 4 flushes now. And now some kind of sprout is  coming up. it's day 13 after spawning and still it's not 100% colonized. It's more like 50% colonized. i think i have the moisture in the sub right. the temperature is 81oF, 95%RH, 12/12 6500K light. it's pan cyan jam in a tray covered in plastic wrap with 6 small holes poked in it. it's a hpoo substrate...2:1 spawn to sub ratio.  my poo was just sun dried and not aged or composted. i think that's why it's not colonizing well. i still have a bunch of other manures i want to try out.

doing agar to grain tonight on the aussie print. pressure cooking my grain for that now.

i was thinking about using my grain water (from the boiled rye) to hydrate the sub. maybe i this can be used to help the myc colonize the substrate. i know this will make it more prone to contam, but i plan to use synthetic filter discs for air exchange during colonization of the sub. i want to try it out and see what happens.

i'm also going to do some tests with mixing vs layering for combining the spawn and substrate. i hear layering has better performance. i've been mixing them so far.

i want to grow some cubes sometime too. at least i know they'll grow...haha.

 



I think they got u bro


--------------------
Natural omt/detox
online  pant cult classes available last Saturday of every month go to buymeacoffee.com/jakeoncid to sign up (1 on 1 consultations also available
JOC PAN TEK
CORDYCEPS MILITARIS

EXOTICS
[


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Offlineshroomms
Mr. Shrooms

Registered: 07/27/14
Posts: 202
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Re: Official Copelandia/Panaeolus Thread *DELETED* [Re: Jakeoncid419]
    #25795160 - 02/06/19 01:24 PM (4 years, 11 months ago)

Post deleted by shroomms

Reason for deletion: I wanted to! Thank-you!


Edited by shroomms (02/06/19 01:32 PM)


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OfflineRad Aghast
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Registered: 12/07/18
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Loc: Minnesota
Last seen: 4 months, 29 days
Re: Official Copelandia/Panaeolus Thread [Re: shroomms]
    #25795184 - 02/06/19 01:32 PM (4 years, 11 months ago)

Quote:

shroomms said:
Every morning i'm harvesting another flush of these parasol mushrooms...






I'm not convinced. So many phenotype variations...


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Offlineshroomms
Mr. Shrooms

Registered: 07/27/14
Posts: 202
Last seen: 1 month, 6 days
Re: Official Copelandia/Panaeolus Thread *DELETED* [Re: Rad Aghast]
    #25795187 - 02/06/19 01:34 PM (4 years, 11 months ago)

Post deleted by shroomms

Reason for deletion: I wanted to! Thank-you!


Edited by shroomms (02/06/19 01:35 PM)


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OfflineJakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: shroomms]
    #25795200 - 02/06/19 01:42 PM (4 years, 11 months ago)

Quote:

shroomms said:
haha

I should eat some of them and see what happens  j/k. They don't bruise blue.

I just collected a nice pile of hpoo from the neighbor's horse. I don't know what he does with all the poo, but this spring and summer I might start a compost pile...i'll have to ask what he does with all the poop and see if i can have it. maybe the poo i used was too old.



well there are active species that don't bruise blue (tamps for example) but I don't think what you go there is active.... but I know very little of identification. mushroom hunting and identification is not something I have every really done. I can identify a good bit of actives and a few gourmet but that about it. some ppl know the names and traits of thousands of mushrooms its incredible but I have no real desire to study and learn all that Id rather research species that I am interested in. but yeah I would imagine that they just printed a random mushroom bulked it and sold it online as a pan kit for a quick but. either that or they have no idea what they are doing... u said there is a sprout in there? like a plant? lol
there are not a lot of backwards steps in mycology. often times when you make a mistake you need to start over. that's why you always make more jars than you think u will need.


--------------------
Natural omt/detox
online  pant cult classes available last Saturday of every month go to buymeacoffee.com/jakeoncid to sign up (1 on 1 consultations also available
JOC PAN TEK
CORDYCEPS MILITARIS

EXOTICS
[


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OfflineRad Aghast
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Re: Official Copelandia/Panaeolus Thread [Re: shroomms]
    #25795203 - 02/06/19 01:43 PM (4 years, 11 months ago)

Not at all. Maybe I missed something, but I just haven't seen a real convincing argument for why those aren't pans. :tongue:

You could EAT some...  :grin:


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Offlineshroomms
Mr. Shrooms

Registered: 07/27/14
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Re: Official Copelandia/Panaeolus Thread *DELETED* [Re: Rad Aghast]
    #25795214 - 02/06/19 01:47 PM (4 years, 11 months ago)

Post deleted by shroomms

Reason for deletion: I wanted to! Thank-you!


Edited by shroomms (02/06/19 01:54 PM)


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OfflineJakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: shroomms]
    #25795230 - 02/06/19 01:53 PM (4 years, 11 months ago)

Quote:

shroomms said:
Quote:

Rad Aghast said:
Not at all. Maybe I missed something, but I just haven't seen a real convincing argument for why those aren't pans. :tongue:

You could EAT some...  :grin:




haha. i don't know if it's safe; i don't want to get poisoned.  i don't know. do they look like pans? they're pretty small.

are these pans (supposedly pan cyan jam)? They're fruiting every day even though it's still in incubation mode and i didn't case it.
  http://

and i keep throwing them in the trash..haha



pans can be small. what species are they supposed to be?


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Offlineshroomms
Mr. Shrooms

Registered: 07/27/14
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Re: Official Copelandia/Panaeolus Thread *DELETED* [Re: Jakeoncid419]
    #25795234 - 02/06/19 01:55 PM (4 years, 11 months ago)

Post deleted by shroomms

Reason for deletion: I wanted to! Thank-you!


Edited by shroomms (02/06/19 01:56 PM)


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