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Invisiblemary fairchild
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Registered: 12/06/14
Posts: 777
Trusted Cultivator
Re: Official Copelandia/Panaeolus Thread [Re: SunnyDayze]
    #25762115 - 01/22/19 11:37 PM (5 years, 7 days ago)

Quote:

SunnyDayze said:
Quote:

mary fairchild said:
Nothing special about my GH, its just one of those indoor orchid growing mini greenhouses. Not as dialed in as i'd like and relatively low tek compared to most of them. Here's a pic of the bottom part of it:

So on the bottom left is a Sunbeam wicking humidifier- got it at Walmart that is on all the time- it blows humid air (~85%RH) from below thru the GH. On the right is an ultrasonic I got from Amazon that ups the humidity to ~99%. The US is slaved to a humidity controller (Inkbird brand). So theres constant air flow from the wicking humidifier and high humidity from the ultrasonic. Temperature is mostly controlled from a couple of seedling heat mats. Its all in a closet. Air from below is probably not the best way to go & I mean to make changes at some point but its worked ok for me anyway.
Blue Helix has the best setup and is the best source for info- he's got things dialed in better than anyone else..



Quote:

Jakeoncid419 said:
Quote:

SunnyDayze said:
Awesome, I love anything I can set and forget.  I’m really good at the forget part so I’ve found the sous vides to be a real good tool for me.



Sous video? And yeah the pasteurization is set in forget however the pans themselves  will require lots of attention I check the moisture of my sub 3-5 times a day (probably overkill but I have my fae way up to keep my evaporation constant) but u gotta be careful doesn’t take long to fry em. Good luck brother feel free to hit me up if u got questions




Auto correct doesn’t like the word SOUS VIDES.  I plan on making a greenhouse just like Mary’s up above and using the sous vides in a pot of water in the bottom to keep up the heat and humidity.  Not sure how well it will work but might as well try. I’m sure it will be perfect for pasteurizing anyway.  Maybe they can start marketing immersion cookers to mycologists and not just chefs lol
Thanks for the help Jake!  Once I get going I’ll take you up on your offer for more help!  I love this place! 💗




One thing I would not do is have all the heat inside the GH so there is a big temperature difference between the GH and the outside. Best to heat the room the GH is in.
otherwise you will get lots of condensation and dripping inside the GH. I like the GH in a closet so I don't have to heat such a big space.


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Invisiblemary fairchild
Pantheist


Registered: 12/06/14
Posts: 777
Trusted Cultivator
Re: Official Copelandia/Panaeolus Thread [Re: Brain Bulb]
    #25762118 - 01/22/19 11:41 PM (5 years, 7 days ago)

Quote:

Brain Bulb said:
How do you think this tray of Aussies is looking.  Ready for casing?









Looks really good but not complete coverage yet, i'd wait till its uniform across the top, then case & go to fruiting.


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OfflineJakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: mary fairchild]
    #25762206 - 01/23/19 01:28 AM (5 years, 7 days ago)

got a new air pump, heater and a 5 pack of humidity/tempature meters on the way. gonna mod my tub set up even further. with the pump and the o2 condenser I should be able to make all my needed fae internally. the room fan is dropping my temp down more than id like now that its really cold outside and I cant afford to hold temp off house heat. I have two different idea's and im not quite sure which would be better maybe yall can offer sum advice... so I could suck in warm dry air (pump set on a repeat cycle time) which ill have set to blow across the sub. which would cause more evaporation, however I would then need to have the humidifier cranked (honestly I would need to redesign setup of humidifier) to be able to rehydrate the sub so it doesn't get fired. I don't know if this would be to drastic... would I be hitting a point of diminished returns on the "more evaporation the better" concept? my other (and the one im prolly gonna do) is to run the pump into the condensation chamber to come in pre hydrated (like you do in a commercial grow space) this would be less overall evaporation of water from the sub but it would be much less stressfull I feel on the substrate..... anyway what yall think?


--------------------
Natural omt/detox
online  pant cult classes available last Saturday of every month go to buymeacoffee.com/jakeoncid to sign up (1 on 1 consultations also available
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OfflineShaperDreaming
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Re: Official Copelandia/Panaeolus Thread [Re: Caps McGee]
    #25762932 - 01/23/19 10:19 AM (5 years, 7 days ago)

Quote:

Caps McGee said:
Try a softer agar perhaps... help hydrate the spores



What recipe do you recommend for this? How much agar per 400ml?


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Invisiblenube424
Male

Registered: 12/03/17
Posts: 6,063
Re: Official Copelandia/Panaeolus Thread [Re: ShaperDreaming]
    #25762956 - 01/23/19 10:28 AM (5 years, 7 days ago)

6g for 400ml would already be soft. U could try 5 grams tho. But I just germed asuras print on a dry batch of agar. It sat unused for like 3 weeks and dried out a little.

Honestly, if ur having trouble, scrape a tiny clump off spores on a scalpel and run the blade through the agar so the spores are below the surface a little.. it's moister there and it works for stubborn spores.


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OfflineShaperDreaming
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Re: Official Copelandia/Panaeolus Thread [Re: nube424]
    #25762972 - 01/23/19 10:36 AM (5 years, 7 days ago)

Quote:

nube424 said:
6g for 400ml would already be soft. U could try 5 grams tho. But I just germed asuras print on a dry batch of agar. It sat unused for like 3 weeks and dried out a little.

Honestly, if ur having trouble, scrape a tiny clump off spores on a scalpel and run the blade through the agar so the spores are below the surface a little.. it's moister there and it works for stubborn spores.



My inoc loop wedged some spores under the surface of the pan trop plate... I can see them in there. Still nothing :frown: What if I were to inject from syringe INTO the agar?

And thanks, yeah, the agar at this recipe was pretty "soft" I thought, as the media bottle JIGGLED coming out of the fridge. :shrug:

I'm begining to think I may need to figure out either a warm germinating space (I'm moving in a month, so that may happen)... or just wait until like late April? DAMN!


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Invisiblenube424
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Re: Official Copelandia/Panaeolus Thread [Re: ShaperDreaming]
    #25762974 - 01/23/19 10:39 AM (5 years, 7 days ago)

Hmm. U could try making an of to germinate it and clean it on agar after? Or just wait...


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Offlinemullugh
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Re: Official Copelandia/Panaeolus Thread [Re: ShaperDreaming]
    #25762975 - 01/23/19 10:39 AM (5 years, 7 days ago)

My two pan trop modified shoe boxes.



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Offlinemullugh
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Re: Official Copelandia/Panaeolus Thread [Re: mullugh]
    #25762982 - 01/23/19 10:42 AM (5 years, 7 days ago)

The whole set up



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Invisiblerickyswamps
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Registered: 11/08/18
Posts: 1,192
Re: Official Copelandia/Panaeolus Thread [Re: mullugh]
    #25762992 - 01/23/19 10:46 AM (5 years, 7 days ago)

What do people like the best: sterilize a substrate mix and inoculate that.  Or pasteurize the substate and mix with colonized grains?


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Invisiblenube424
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Re: Official Copelandia/Panaeolus Thread [Re: rickyswamps]
    #25763022 - 01/23/19 10:59 AM (5 years, 7 days ago)

I like the 2nd. That's just what I'm used to tho. I'm sure if I tried the pf style containers it has alot of benefits.  The inoculation part is what stumps me with that because I use agar not lc. I guess I could make an lc.


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Offlinemullugh
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Re: Official Copelandia/Panaeolus Thread [Re: rickyswamps]
    #25763023 - 01/23/19 11:00 AM (5 years, 7 days ago)

Quote:

rickyswamps said:
What do people like the best: sterilize a substrate mix and inoculate that.  Or pasteurize the substate and mix with colonized grains?




The latter, but I'm a novice with pans


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InvisibleJ. Jack Flash
stranger than ever.

Registered: 11/20/13
Posts: 1,500
Re: Official Copelandia/Panaeolus Thread [Re: rickyswamps]
    #25763024 - 01/23/19 11:01 AM (5 years, 7 days ago)

Quote:

ShaperDreaming said:
Quote:

nube424 said:
6g for 400ml would already be soft. U could try 5 grams tho. But I just germed asuras print on a dry batch of agar. It sat unused for like 3 weeks and dried out a little.

Honestly, if ur having trouble, scrape a tiny clump off spores on a scalpel and run the blade through the agar so the spores are below the surface a little.. it's moister there and it works for stubborn spores.



My inoc loop wedged some spores under the surface of the pan trop plate... I can see them in there. Still nothing :frown: What if I were to inject from syringe INTO the agar?

And thanks, yeah, the agar at this recipe was pretty "soft" I thought, as the media bottle JIGGLED coming out of the fridge. :shrug:

I'm begining to think I may need to figure out either a warm germinating space (I'm moving in a month, so that may happen)... or just wait until like late April? DAMN!




if you already got plates inoculated, and a gentle heat source people are saying not to use, and you're considering waiting three solid months, why not try using the thing people say not to use, if you're just going to be waiting anyway? what's a seed starting mat but a heating pad?

put another way, before time became so precious (read: scarce) for me, i used to make yogurt. like, lots of yogurt. gallon or two a week. temp controller on a heating pad inside a middle sized cooler held 115F +/-2 no sweat. if you don't have a temp controller, get one. they're cheap and there's all kinds of things you can do with them. anyway, on this basis, you could just barely heat the inside of a cooler, or even just a cardboard box, to 75 or 80F, with a small tray to hold the plates off the mat.

on the other hand, time could make it happen without added heat. one reason i'm loving this mushcult business so much is that waiting is active participation. good luck.


--------------------
 
the j stands for jesus.
2020 new years grow along


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Offlinemullugh
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Re: Official Copelandia/Panaeolus Thread [Re: nube424]
    #25763025 - 01/23/19 11:01 AM (5 years, 7 days ago)

Quote:

nube424 said:
I like the 2nd. That's just what I'm used to tho. I'm sure if I tried the pf style containers it has alot of benefits.  The inoculation part is what stumps me with that because I use agar not lc. I guess I could make an lc.



Yeah, I only use agar. Tried to master lc's and it was hit and miss so I have that up


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OfflineShaperDreaming
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Re: Official Copelandia/Panaeolus Thread [Re: J. Jack Flash]
    #25763041 - 01/23/19 11:07 AM (5 years, 7 days ago)

Quote:

J. Jack Flash said:
if you already got plates inoculated, and a gentle heat source people are saying not to use, and you're considering waiting three solid months, why not try using the thing people say not to use, if you're just going to be waiting anyway? what's a seed starting mat but a heating pad?

put another way, before time became so precious (read: scarce) for me, i used to make yogurt. like, lots of yogurt. gallon or two a week. temp controller on a heating pad inside a middle sized cooler held 115F +/-2 no sweat. if you don't have a temp controller, get one. they're cheap and there's all kinds of things you can do with them. anyway, on this basis, you could just barely heat the inside of a cooler, or even just a cardboard box, to 75 or 80F, with a small tray to hold the plates off the mat.

on the other hand, time could make it happen without added heat. one reason i'm loving this mushcult business so much is that waiting is active participation. good luck.



Yes, waiting is fine with me! I've grown a TON of slow moving things. I think they're fun.

This is a good point... I think I'm going to just put these fuckers in a cardboard box over the heating mat. They're only on 12 hours a day, so it won't be too bad no matter what. What's the worst that happens, nothing? Already got that result :lol:


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OfflineJakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: ShaperDreaming]
    #25763068 - 01/23/19 11:18 AM (5 years, 7 days ago)

AAAAGHHHEMMM! lol I need sum feed back you guys.... starting the design today, where yatt?


--------------------
Natural omt/detox
online  pant cult classes available last Saturday of every month go to buymeacoffee.com/jakeoncid to sign up (1 on 1 consultations also available
JOC PAN TEK
CORDYCEPS MILITARIS

EXOTICS
[


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Invisiblenube424
Male

Registered: 12/03/17
Posts: 6,063
Re: Official Copelandia/Panaeolus Thread [Re: ShaperDreaming]
    #25763072 - 01/23/19 11:21 AM (5 years, 7 days ago)

Hey guys. What do u think of this? Pan bisporus, one is cased with cactus soil and the there's 2 others getting cased today.



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InvisibleJ. Jack Flash
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Registered: 11/20/13
Posts: 1,500
Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
    #25763092 - 01/23/19 11:33 AM (5 years, 7 days ago)

Quote:

Jakeoncid419 said:
AAAAGHHHEMMM! lol I need sum feed back you guys.... starting the design today, where yatt?




ahahaha sorry man, you're on some next-level shit with the works you got going. wouldn't know where to begin.

re: evaporation as pinning trigger, i was wondering about this recently. it's not like fungus has a nervous system, so how does it sense evaporation? it must sense the associated temperature drop, or heat carried away by the evaporating water. sorta like how i hear shitake are fruited starting with a cold shock, i reckon it's similar. whether there's a point of diminishing returns, i'd wager there must be. and maybe even a tipping point of too much evap for comfort.

i'd try the humidify-the-pumped-air option first.


--------------------
 
the j stands for jesus.
2020 new years grow along


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OfflineAppalachian Brony
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Re: Official Copelandia/Panaeolus Thread [Re: rickyswamps]
    #25763104 - 01/23/19 11:40 AM (5 years, 7 days ago)

I’ve failed with cyan and bisporus doin sterilized sub both broken up into trays and fruited from the container like pancakes. Was surely user error regarding one or more of a few different things like too much grain in the mix, too moist, or bad growing conditions though.

Last year I used the same bisporus culture for sterilized sub and grains, the broken up sub didn’t fruit, the straight cased ziploc pints didnt fruit, but the grain spawned to pasteurized sub did.

That’s just my experience though, the best flushes that I’ve seen on here are from Mary, Asura, and BlueHelix and they all seem to do the sterilized sub method along with greenhouses.


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InvisibleSunnyDayze
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Re: Official Copelandia/Panaeolus Thread [Re: mary fairchild]
    #25763116 - 01/23/19 11:52 AM (5 years, 7 days ago)

Quote:

mary fairchild said:
Quote:

SunnyDayze said:
Quote:

mary fairchild said:
Nothing special about my GH, its just one of those indoor orchid growing mini greenhouses. Not as dialed in as i'd like and relatively low tek compared to most of them. Here's a pic of the bottom part of it:

So on the bottom left is a Sunbeam wicking humidifier- got it at Walmart that is on all the time- it blows humid air (~85%RH) from below thru the GH. On the right is an ultrasonic I got from Amazon that ups the humidity to ~99%. The US is slaved to a humidity controller (Inkbird brand). So theres constant air flow from the wicking humidifier and high humidity from the ultrasonic. Temperature is mostly controlled from a couple of seedling heat mats. Its all in a closet. Air from below is probably not the best way to go & I mean to make changes at some point but its worked ok for me anyway.
Blue Helix has the best setup and is the best source for info- he's got things dialed in better than anyone else..



Quote:

Jakeoncid419 said:
Quote:

SunnyDayze said:
Awesome, I love anything I can set and forget.  I’m really good at the forget part so I’ve found the sous vides to be a real good tool for me.



Sous video? And yeah the pasteurization is set in forget however the pans themselves  will require lots of attention I check the moisture of my sub 3-5 times a day (probably overkill but I have my fae way up to keep my evaporation constant) but u gotta be careful doesn’t take long to fry em. Good luck brother feel free to hit me up if u got questions




Auto correct doesn’t like the word SOUS VIDES.  I plan on making a greenhouse just like Mary’s up above and using the sous vides in a pot of water in the bottom to keep up the heat and humidity.  Not sure how well it will work but might as well try. I’m sure it will be perfect for pasteurizing anyway.  Maybe they can start marketing immersion cookers to mycologists and not just chefs lol
Thanks for the help Jake!  Once I get going I’ll take you up on your offer for more help!  I love this place! 💗




One thing I would not do is have all the heat inside the GH so there is a big temperature difference between the GH and the outside. Best to heat the room the GH is in.
otherwise you will get lots of condensation and dripping inside the GH. I like the GH in a closet so I don't have to heat such a big space.




I do have a couple of options of where i put the GH  but the best would be right around my grow area.  I put a heater in my room to stop my plates from condensing so bad by keeping the temp steadier.  It ranges from 20-22 c (68-72 f).  I can keep it closer to 22-24 c if i have to but its my bedroom lol  (the blinding lights are my alarm in the mornings).

Dew Point Calculator tells me that i need to have the same temperature at 100% rh.  1.5 degrees less at 95% rh.  What is the lowest temp I should keep in the GH?

@Jake, sorry, I'd help if I could!


--------------------


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