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InvisibleCaps McGee
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Re: Official Copelandia/Panaeolus Thread [Re: ShaperDreaming]
    #25760754 - 01/22/19 12:01 PM (5 years, 8 days ago)

Patience... spores can take weeks to germinate sometimes


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Invisiblerickyswamps
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Registered: 11/08/18
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Re: Official Copelandia/Panaeolus Thread [Re: ShaperDreaming]
    #25760756 - 01/22/19 12:02 PM (5 years, 8 days ago)

I don't have a problem germinating them and use the same agar recipe.  Usually in 3 days I see something.  How many drops are putting on the agar out of the syringe?  Sometimes too much is not good.  Too much water on the plate.  I'm assuming you are shaking it like crazy before flaming to get the spores mixed up.


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Invisiblenube424
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Re: Official Copelandia/Panaeolus Thread [Re: ShaperDreaming]
    #25760760 - 01/22/19 12:03 PM (5 years, 8 days ago)

Is it cold where u are? My pans didn't germinate till I put them in a warmer spot.


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OfflineShaperDreaming
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Re: Official Copelandia/Panaeolus Thread [Re: Caps McGee]
    #25760764 - 01/22/19 12:06 PM (5 years, 8 days ago)

Quote:

rickyswamps said:
I don't have a problem germinating them and use the same agar recipe.  Usually in 3 days I see something.  How many drops are putting on the agar out of the syringe?  Sometimes too much is not good.  Too much water on the plate.  I'm assuming you are shaking it like crazy before flaming to get the spores mixed up.



I am shaking. I use an inoculation loop, so less than a drop. This last batch did get a ton of condensation on the agar itself though... that may be the culprit.
Quote:

nube424 said:
Is it cold where u are? My pans didn't germinate till I put them in a warmer spot.



Yes, but my closet doesn't dip below 68f-72f, should I put them somewhere warmer?
Quote:

Caps McGee said:
Patience... spores can take weeks to germinate sometimes



Trying caps! I already failed at this once, starting to take it personally.


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Invisiblenube424
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Re: Official Copelandia/Panaeolus Thread [Re: ShaperDreaming]
    #25760782 - 01/22/19 12:11 PM (5 years, 8 days ago)

Pans like warmth. They're tropical. Do u have any space in ur house that's warmer than the rest?

Possibly next to a heater or on a shelf above ur fridge? Where ever there is a warm spot. Don't go making an incubator. Just a regular spot that's warmer than the rest.

Pans and cubes are completely different.


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OfflineShaperDreaming
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Re: Official Copelandia/Panaeolus Thread [Re: nube424]
    #25760791 - 01/22/19 12:14 PM (5 years, 8 days ago)

Quote:

nube424 said:
Pans like warmth. They're tropical. Do u have any space in ur house that's warmer than the rest?

Possibly next to a heater or on a shelf above ur fridge? Where ever there is a warm spot. Don't go making an incubator. Just a regular spot that's warmer than the rest.

Pans and cubes are completely different.



The only thing that may work is a seed warming shelf I use for my succulents. Would that be unwise since it's not a "warm area" but a "warm surface"?

And for "warmer" spots... I'm in an apartment with radiators, so it's complicated. The answer is likely "no" unless my seed shelf works.


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Invisiblenube424
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Re: Official Copelandia/Panaeolus Thread [Re: ShaperDreaming]
    #25760800 - 01/22/19 12:19 PM (5 years, 8 days ago)

Definitely DON'T put them on a seedling Mat.

U can't put them near the radiator somewhere?  My pan agar dishes are in a nightstand in front of a radiator. Stays warmer inside there than anywhere else in my house right now.

All my cubes are in they're normal spot and still doing fine. But my pans wouldn't germinate in said spot.


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OfflineBrain Bulb
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Re: Official Copelandia/Panaeolus Thread [Re: nube424] * 1
    #25760814 - 01/22/19 12:25 PM (5 years, 8 days ago)

My grain jar of Pan. Bisporus is starting to colonize!:-).  These are from last night.



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OfflineJakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: Caps McGee]
    #25760831 - 01/22/19 12:34 PM (5 years, 8 days ago)

Quote:

Caps McGee said:
Patience... spores can take weeks to germinate sometimes



cube prints sure and I could be wrong but I have hit hundreds of dishes with pans over the last year and I always saw germ within 5 days (usually 3)


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OfflineShaperDreaming
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Re: Official Copelandia/Panaeolus Thread [Re: nube424]
    #25760834 - 01/22/19 12:35 PM (5 years, 8 days ago)

Quote:

nube424 said:
Definitely DON'T put them on a seedling Mat.

U can't put them near the radiator somewhere?  My pan agar dishes are in a nightstand in front of a radiator. Stays warmer inside there than anywhere else in my house right now.




Yeah, I assumed. Will not do!

As for the other part, all my radiators are near very drafty windows. If I put it there the temp would swing wildly. My building is over 100 years old :frown:

I'll try to get creative.


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Invisiblerickyswamps
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Re: Official Copelandia/Panaeolus Thread [Re: ShaperDreaming]
    #25760852 - 01/22/19 12:41 PM (5 years, 8 days ago)

I go directly from syringe to plate and it works.  Sometimes use a loop to spread them, sometimes not.  Found they germinate fine without doing anything.  2 drop maximum. I am not sure if putting a drop on an inoculation loop and then on plate could lead to not having enough spores?


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Invisiblenube424
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Re: Official Copelandia/Panaeolus Thread [Re: ShaperDreaming]
    #25760875 - 01/22/19 12:52 PM (5 years, 8 days ago)

Quote:

ShaperDreaming said:
Quote:

nube424 said:
Definitely DON'T put them on a seedling Mat.

U can't put them near the radiator somewhere?  My pan agar dishes are in a nightstand in front of a radiator. Stays warmer inside there than anywhere else in my house right now.




Yeah, I assumed. Will not do!

As for the other part, all my radiators are near very drafty windows. If I put it there the temp would swing wildly. My building is over 100 years old :frown:

I'll try to get creative.



How hot does the mat get? Maybe u could put a cookie tray standing over the mat with a cardboard box overturned on top to hold in some heat, then put ur plates on there.

Worth trying instead of throwing out plates. I have to get creative in winter too. Living in the cold north sucks. EVERYTHING'S covered in ice right now lol


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OfflineShaperDreaming
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Re: Official Copelandia/Panaeolus Thread [Re: nube424]
    #25760914 - 01/22/19 01:14 PM (5 years, 8 days ago)

Quote:

nube424 said:
How hot does the mat get? Maybe u could put a cookie tray standing over the mat with a cardboard box overturned on top to hold in some heat, then put ur plates on there.

Worth trying instead of throwing out plates. I have to get creative in winter too. Living in the cold north sucks. EVERYTHING'S covered in ice right now lol



It says it warms up to 85f, then cools down to 70f, then warms back up... etc.

And yes, it's ice-y out there now... debating on just holding off on this species for a bit lol.


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Invisiblenube424
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Re: Official Copelandia/Panaeolus Thread [Re: ShaperDreaming]
    #25760963 - 01/22/19 01:44 PM (5 years, 8 days ago)

Space heater?


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InvisibleSunnyDayze
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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
    #25761036 - 01/22/19 02:18 PM (5 years, 7 days ago)

Quote:

mary fairchild said:
Nothing special about my GH, its just one of those indoor orchid growing mini greenhouses. Not as dialed in as i'd like and relatively low tek compared to most of them. Here's a pic of the bottom part of it:

So on the bottom left is a Sunbeam wicking humidifier- got it at Walmart that is on all the time- it blows humid air (~85%RH) from below thru the GH. On the right is an ultrasonic I got from Amazon that ups the humidity to ~99%. The US is slaved to a humidity controller (Inkbird brand). So theres constant air flow from the wicking humidifier and high humidity from the ultrasonic. Temperature is mostly controlled from a couple of seedling heat mats. Its all in a closet. Air from below is probably not the best way to go & I mean to make changes at some point but its worked ok for me anyway.
Blue Helix has the best setup and is the best source for info- he's got things dialed in better than anyone else..



Quote:

Jakeoncid419 said:
Quote:

SunnyDayze said:
Awesome, I love anything I can set and forget.  Iโ€™m really good at the forget part so Iโ€™ve found the sous vides to be a real good tool for me.



Sous video? And yeah the pasteurization is set in forget however the pans themselves  will require lots of attention I check the moisture of my sub 3-5 times a day (probably overkill but I have my fae way up to keep my evaporation constant) but u gotta be careful doesnโ€™t take long to fry em. Good luck brother feel free to hit me up if u got questions




Auto correct doesnโ€™t like the word SOUS VIDES.  I plan on making a greenhouse just like Maryโ€™s up above and using the sous vides in a pot of water in the bottom to keep up the heat and humidity.  Not sure how well it will work but might as well try. Iโ€™m sure it will be perfect for pasteurizing anyway.  Maybe they can start marketing immersion cookers to mycologists and not just chefs lol
Thanks for the help Jake!  Once I get going Iโ€™ll take you up on your offer for more help!  I love this place! ๐Ÿ’—


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Offlinemullugh
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Re: Official Copelandia/Panaeolus Thread [Re: Hacendado]
    #25761081 - 01/22/19 02:43 PM (5 years, 7 days ago)

Quote:

Hacendado said:
What kind of pH tester did you get?




This two pronged thing with a little dial and a traffic light system that also measures moisture (for gardens?


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OfflineAsuraS
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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
    #25761504 - 01/22/19 05:56 PM (5 years, 7 days ago)

Quote:

Jakeoncid419 said:
Quote:

Caps McGee said:
Patience... spores can take weeks to germinate sometimes



cube prints sure and I could be wrong but I have hit hundreds of dishes with pans over the last year and I always saw germ within 5 days (usually 3)




You're absolutely right for fresh prints. I have had some vendor pans take 11-13 days before. You know you're
good when it germs in 3.


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OfflineRad Aghast
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Re: Official Copelandia/Panaeolus Thread [Re: ShaperDreaming]
    #25761924 - 01/22/19 09:19 PM (5 years, 7 days ago)

Quote:

ShaperDreaming said:
So, I'm having a HORRIBLE time getting my spores to do anything on Agar. I am sitting at 8 days with no myc. Pan Trop (India) and Pan. Cyan (Jamaican) on agar recipe 400ml water, 6g agar, 6g lme. Other myc (cubes and oysters) love this recipe.

Should I just wait? Jake mentioned something to add that starts with a "P"... do I need that? Should I just go straight syringe->grain? Any advice?




I mentioned having this same problem a few pages back. Some folks recommending more solution, some recommending less, a few others mentioned having similar issues. I put spores from a syringe on MEA and kept them incubated at 80F and got nothing after 15 days. Who wants to wait for a month to find out you got nothin? So I went in a couple different directions. I put down another plate with a small amount of solution, a plate with a large amount of solution, and I inoculated GWLC I made from oats. This was three days ago, and I've already got signs of germination in the GWLC, nothin on plates as usual.

I might have nothing but contams in that GWLC, so I'll have to go back to plates, but this might be something you could try (spores->LC->agar->grains) as it seems to have gotten me past a stuck point. These are with sponsor's Hawaiians BTW.


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InvisibleCaps McGee
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Re: Official Copelandia/Panaeolus Thread [Re: Rad Aghast]
    #25762071 - 01/22/19 10:57 PM (5 years, 7 days ago)

Try a softer agar perhaps... help hydrate the spores


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OfflineBrain Bulb
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Re: Official Copelandia/Panaeolus Thread [Re: Rad Aghast]
    #25762088 - 01/22/19 11:11 PM (5 years, 7 days ago)

How do you think this tray of Aussies is looking.  Ready for casing?






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