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Invisiblerickyswamps
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Re: Official Copelandia/Panaeolus Thread [Re: rickyswamps]
    #25754095 - 01/19/19 04:27 PM (5 years, 10 days ago)

Could I get some experienced eyes on this Pan grain jar.  I don't know what I am looking for….is it clean and nice?  Its a Jamaican variety from a well respected vendor.  I put it on Agar, cleaned it up, nocc'd up this one on rye on 12/30.  Its now growing up the walls lol. 





What are some indicators of good quality (ie smell, look, ease of breaking up after shaking, etc…)?  If this jar passes, then I'll post a few more that are looking more suspect...


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OfflineRad Aghast
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Re: Official Copelandia/Panaeolus Thread [Re: rickyswamps]
    #25754216 - 01/19/19 05:38 PM (5 years, 10 days ago)

Sounds like I chose the more stubborn variety. :rolleyes:

I think I will start over and try the MS syringe to grain(oat)-water LC. If that doesn't work I will take Jake up on the print offer.  :bowdown:


I've never prepped GWLC myself - but based on my research here it looks like a 1:4 ratio of the remnants of the grain soak to water, PC'd for 90-120 mts... sound about right?

thx again


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OfflineJakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: rickyswamps]
    #25754219 - 01/19/19 05:41 PM (5 years, 10 days ago)

Quote:

rickyswamps said:
Could I get some experienced eyes on this Pan grain jar.  I don't know what I am looking for….is it clean and nice?  Its a Jamaican variety from a well respected vendor.  I put it on Agar, cleaned it up, nocc'd up this one on rye on 12/30.  Its now growing up the walls lol. 





What are some indicators of good quality (ie smell, look, ease of breaking up after shaking, etc…)?  If this jar passes, then I'll post a few more that are looking more suspect...



honestly the myc looks thicker than i like to see my pan myc. all my thick mycelium jars give me nothing but overlay. my best preforming spawn has thins wispy growth and the grain falls apart with a few light shakes, and of course you want that good mushroom smell. and yes i too failed for several months on pans before i had any success hang in there


--------------------
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Edited by Jakeoncid419 (01/19/19 05:42 PM)


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Offlinespace_shrooms

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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
    #25754222 - 01/19/19 05:43 PM (5 years, 10 days ago)

Pulled a plate of jam out of the fridge after it had been in there about 3-4 weeks. It has taken over a week to start growth again on a new dish. Thought it was dead. Is this normal for mycelium recovery coming out of the cold?


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OfflineAsuraS
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Re: Official Copelandia/Panaeolus Thread [Re: rickyswamps]
    #25754378 - 01/19/19 06:45 PM (5 years, 10 days ago)

Quote:

rickyswamps said:
Could I get some experienced eyes on this Pan grain jar.  I don't know what I am looking for….is it clean and nice?  Its a Jamaican variety from a well respected vendor.  I put it on Agar, cleaned it up, nocc'd up this one on rye on 12/30.  Its now growing up the walls lol. 





What are some indicators of good quality (ie smell, look, ease of breaking up after shaking, etc…)?  If this jar passes, then I'll post a few more that are looking more suspect...




Looks like the nasty pan jams I had that did nothing but overlay.



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Invisiblemary fairchild
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Re: Official Copelandia/Panaeolus Thread [Re: Asura] * 1
    #25754539 - 01/19/19 07:52 PM (5 years, 10 days ago)

As far as Jams go- I was not able to link any particular phenotype on agar to a tendency to overlay. I've had cottony forms that did not overlay as well as those that did; and thinner forms that overlaid as well.
https://www.shroomery.org/forums/showflat.php/Number/24820407

Its just that a particular batch of Jams from a "well respected vendor" has a tendency to overlay. Its the genetics of that batch.
I had a grow from that batch that overlaid badly but it still produced pretty good:


I'd say those jars look good but not quite at 100%. I'd not worry about how thick the growth is.


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OfflineJakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: mary fairchild]
    #25754585 - 01/19/19 08:20 PM (5 years, 10 days ago)

Quote:

mary fairchild said:
As far as Jams go- I was not able to link any particular phenotype on agar to a tendency to overlay. I've had cottony forms that did not overlay as well as those that did; and thinner forms that overlaid as well.
https://www.shroomery.org/forums/showflat.php/Number/24820407

Its just that a particular batch of Jams from a "well respected vendor" has a tendency to overlay. Its the genetics of that batch.
I had a grow from that batch that overlaid badly but it still produced pretty good:


I'd say those jars look good but not quite at 100%. I'd not worry about how thick the growth is.



mary you said you got fruits after it overlay? did u re-case? or just keep waiting. ive been tossing trays as soon as I see overlay cuz ive never been able to make em shake


--------------------
Natural omt/detox
online  pant cult classes available last Saturday of every month go to buymeacoffee.com/jakeoncid to sign up (1 on 1 consultations also available
JOC PAN TEK
CORDYCEPS MILITARIS

EXOTICS
[


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OfflineBrain Bulb
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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
    #25754659 - 01/19/19 08:53 PM (5 years, 10 days ago)

Jar of GS inoculated with my Aussie LC is ready to go! 



Was hoping my GH would be here by now but it hasn’t delivered so I’ll be going with a glass tray in my SGFC.  Time to get some Aussie to fruit!


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Invisiblerickyswamps
Bad Apple
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Registered: 11/08/18
Posts: 1,192
Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
    #25754676 - 01/19/19 08:59 PM (5 years, 10 days ago)

I'll give the jar a try, not much to lose considering I don't have anything else ready.  Maybe y'all will see a pic in a couple weeks asking if this is overlay lol.

Y'all have a photo of some pan mycelium on agar and/or grain that has preformed well for you? Mary, I saw your Agar in that 2 pheno thread.  Mine was very similar.


Edited by rickyswamps (01/19/19 09:04 PM)


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OfflineJakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: rickyswamps]
    #25754692 - 01/19/19 09:05 PM (5 years, 10 days ago)

Quote:

rickyswamps said:
I'll give the jar a try, not much to lose considering I don't have anything else ready.  Maybe y'all will see a pic in a couple weeks asking if this is overlay lol.

Y'all have a photo of some pan mycelium on agar and/or grain that has preformed well for you? Mary, I saw your Agar in that 2 pheno thread.  Mine was very similar.





--------------------
Natural omt/detox
online  pant cult classes available last Saturday of every month go to buymeacoffee.com/jakeoncid to sign up (1 on 1 consultations also available
JOC PAN TEK
CORDYCEPS MILITARIS

EXOTICS
[


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InvisibleCaps McGee
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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
    #25754726 - 01/19/19 09:19 PM (5 years, 10 days ago)

I've noticed my cube cultures rebound faster if I wait 24 hours for them to warm up before transferring from them... not sure if it's applicable with pans though


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Invisiblemary fairchild
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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
    #25754775 - 01/19/19 09:35 PM (5 years, 10 days ago)

As far as Jams go- I was not able to link any particular phenotype on agar to a tendency to overlay. I've had cottony forms that did not overlay as well as those that did.
https://www.shroomery.org/forums/showflat.php/Number/24820407

Its just that a particular batch of Jams from a "well respected vendor" has a tendency to overlay. Its the genetics of that batch.
I had a grow from that batch that overlaid badly but it still produced pretty good:


I'd say those jars look good but not quite at 100%. I'd not worry about how thick the growth is.
Quote:

Jakeoncid419 said:
Quote:

mary fairchild said:
As far as Jams go- I was not able to link any particular phenotype on agar to a tendency to overlay. I've had cottony forms that did not overlay as well as those that did; and thinner forms that overlaid as well.
https://www.shroomery.org/forums/showflat.php/Number/24820407

Its just that a particular batch of Jams from a "well respected vendor" has a tendency to overlay. Its the genetics of that batch.
I had a grow from that batch that overlaid badly but it still produced pretty good:


I'd say those jars look good but not quite at 100%. I'd not worry about how thick the growth is.



mary you said you got fruits after it overlay? did u re-case? or just keep waiting. ive been tossing trays as soon as I see overlay cuz ive never been able to make em shake




No- I didn't recase- I learned earlier that recasing did NOT help. I think Asura has found the same.

So in that grow ~75% of the case had matted overlay- I dont mean mere colonization of the case- but complete coverage of the case so you couldn't see the case anymore.
Thats what i'm defining as overlay- coverage so you can't see the case anymore.

The thickness of that coverage varied- by ~6 days in fruiting conditions some areas had thick overlay that even started to harden- those thickest areas never yielded pins but the thinner areas had pins breaking right thru the overlay. So I still got a yield but more in clusters where the overlay was thinner.

So the overlay starts thin, then thickens over time, then it becomes hard and eventually often shows blue bruising as the mycelia ages.

The way I look at overlay  is- When the tray is put into fruiting conditions that should signal the mycelia to stop growing and switch to fruiting mode, for whatever reason some of these Jams don't seem to get that signal and the mycelia continues to propagate.
In that picture that I showed I got fruiting by day 6 of fruiting conditions. So some areas of the surface were overlaying while other areas were switching to fruiting mode. The strain from that vendor is prone to overlay but it still could work.

From what i've seen with Pan cyans- if I dont get pins by day 6 then something is wrong with the grow.

I really think this tendency to overlay is a bell curve thing- if you are lucky enough to be at the fringes of that probability curve it could still work well with little or no overlay at all. Anyway I will steer away from that particular strain.
BTW I have plenty of prints of that strain of Jams:eek:


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Invisiblemary fairchild
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Re: Official Copelandia/Panaeolus Thread [Re: Brain Bulb]
    #25754791 - 01/19/19 09:38 PM (5 years, 10 days ago)

Quote:

Brain Bulb said:
Jar of GS inoculated with my Aussie LC is ready to go! 



Was hoping my GH would be here by now but it hasn’t delivered so I’ll be going with a glass tray in my SGFC.  Time to get some Aussie to fruit!




Hi BB!
Like before- really nice looking plates and now nice looking grain jars- once you get your fruiting conditions/ chamber set good things will happen:popcorn:


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Invisiblemary fairchild
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Re: Official Copelandia/Panaeolus Thread [Re: rickyswamps]
    #25754822 - 01/19/19 09:52 PM (5 years, 10 days ago)

Quote:

rickyswamps said:
I'll give the jar a try, not much to lose considering I don't have anything else ready.  Maybe y'all will see a pic in a couple weeks asking if this is overlay lol.

Y'all have a photo of some pan mycelium on agar and/or grain that has preformed well for you? Mary, I saw your Agar in that 2 pheno thread.  Mine was very similar.




I've had pretty monoculture plates fruit well, and pretty plates not do well and for that matter ugly- yellowish looking monoculture colonies on agar end up fruiting well, had pure multispore fruit well.
In other words, trying to link a growth form on agar to fruiting capacity- never found it.


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InvisibleSunnyDayze
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Re: Official Copelandia/Panaeolus Thread [Re: mary fairchild]
    #25754833 - 01/19/19 09:58 PM (5 years, 10 days ago)

I got my jam prints from a respected shroomery vendor.  The mycellium was so fuzzy it was flattening out on the lid of the petris.  I posted on here and someone said they would probably overlay so I threw them out.  I just put more spores to agar a couple of days ago and will try to transfer the thinnest part.
Thanks for the info and link.  Super relevant to me right now.


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Invisiblemary fairchild
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Re: Official Copelandia/Panaeolus Thread [Re: SunnyDayze]
    #25754860 - 01/19/19 10:19 PM (5 years, 10 days ago)

Hi Sunny,
If you got your Jam print recently then its not the vendor in question since they are out of stock.
I had nice cottony forms that didn't overlay (I've seen galindoi mycelia completely fill the petri dish but not Jams- id definitely like to take that one to fruiting just to see what happens), consider making four agar selections from a streaked plate, one of them being a colony with very thick growth and one with thinner growth, the others in between.

ie, eggs in multiple baskets.


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InvisibleSunnyDayze
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Re: Official Copelandia/Panaeolus Thread [Re: mary fairchild]
    #25754903 - 01/19/19 10:52 PM (5 years, 10 days ago)

I will try it, thanks.  I’ll even take pics and document because my memory is not good to say the least.  The prints I got about a month or probably closer to two ago.

My only issue is This is my first grow, so hard to say if it doesn’t turn out if it’s the mycellium or more likely my inexperience lol. But I will give it a go!  Lord hates a coward right?

I did get a little greenhouse I’ll be setting up when ready, following your set up.


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Invisiblemary fairchild
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Re: Official Copelandia/Panaeolus Thread [Re: SunnyDayze]
    #25754995 - 01/19/19 11:57 PM (5 years, 10 days ago)

Sounds good,
I'm happy to help if I can- lets give it a go!


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InvisibleCaps McGee
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Re: Official Copelandia/Panaeolus Thread [Re: mary fairchild]
    #25755151 - 01/20/19 02:42 AM (5 years, 10 days ago)

Simplest substrate for pan cyanescens?


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OfflineJakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: Caps McGee]
    #25755197 - 01/20/19 03:49 AM (5 years, 10 days ago)

Quote:

Caps McGee said:
Simplest substrate for pan cyanescens?



60% chopped straw 35% poo 5% verm is what I use seems to be working well got another mono tub just starting to kick up pan aus pins on that sub.


--------------------
Natural omt/detox
online  pant cult classes available last Saturday of every month go to buymeacoffee.com/jakeoncid to sign up (1 on 1 consultations also available
JOC PAN TEK
CORDYCEPS MILITARIS

EXOTICS
[


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