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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 3 months
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Re: Copelandia cyanescens hyphal knots. [Re: Kalistis]
#22523968 - 11/14/15 07:10 PM (8 years, 2 months ago) |
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So Spacechildo said he exclusively trips on Pans and I did some research and I'm very curious now.
Dropping some on agar tomorrow night
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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Re: Copelandia cyanescens hyphal knots. [Re: taGyo]
#22524901 - 11/14/15 09:29 PM (8 years, 2 months ago) |
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not that much to reserch really, thin subs (~2"), throw some manure or straw in them for better results, up the FAE and temps too, and prepare for lift off!
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Re: Copelandia cyanescens hyphal knots. [Re: spacechildo]
#22525021 - 11/14/15 09:47 PM (8 years, 2 months ago) |
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firstTIMER420
Born the son of a sharecropper..



Registered: 05/08/15
Posts: 1,025
Loc: US, maybe?
Last seen: 6 years, 4 days
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Re: Copelandia cyanescens hyphal knots. [Re: spacechildo]
#22525194 - 11/14/15 10:18 PM (8 years, 2 months ago) |
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Ive always been planning on using half straw half manure, but im thinking about just using strait manure. Would that be better or worse in yalls opinion?
-------------------- 1 2 3 4 http://www.shroomery.org/forums/showflat.php/Number/22386794[/url] --Anything posted by this account is completely fictitious--
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Copelandia cyanescens hyphal knots. [Re: firstTIMER420]
#22525206 - 11/14/15 10:20 PM (8 years, 2 months ago) |
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strait horse manure works great
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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firstTIMER420
Born the son of a sharecropper..



Registered: 05/08/15
Posts: 1,025
Loc: US, maybe?
Last seen: 6 years, 4 days
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Re: Copelandia cyanescens hyphal knots. [Re: cronicr]
#22525212 - 11/14/15 10:21 PM (8 years, 2 months ago) |
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For some reason when I throw straw in there, it contams. I think because I mess up the moisture content. Not sure if because last time it was a really weak strain. But straight horse manure is really easy to get to field capacity with some verm and gypsum in it.
-------------------- 1 2 3 4 http://www.shroomery.org/forums/showflat.php/Number/22386794[/url] --Anything posted by this account is completely fictitious--
Edited by firstTIMER420 (11/14/15 10:22 PM)
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Copelandia cyanescens hyphal knots. [Re: firstTIMER420]
#22525231 - 11/14/15 10:24 PM (8 years, 2 months ago) |
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try add lime next time you use straw
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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LocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ



Registered: 04/17/15
Posts: 7,076
Loc: to the brain
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Re: Copelandia cyanescens hyphal knots. [Re: cronicr]
#22525288 - 11/14/15 10:32 PM (8 years, 2 months ago) |
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Quote:
cronicr said: try add lime next time you use straw
What difference does the lime cause as opposed to gypsum?
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Q&A US vs. THEM The more I learn, the less I know.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Copelandia cyanescens hyphal knots. [Re: LocN9ne]
#22525325 - 11/14/15 10:39 PM (8 years, 2 months ago) |
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lime raises the ph, gypsum stablizes the ph and prevents it from swinging, as it colonizes it becomes more acidic causing the ph to drop, gypsum helps this
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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Re: Copelandia cyanescens hyphal knots. [Re: LocN9ne]
#22525336 - 11/14/15 10:41 PM (8 years, 2 months ago) |
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Lime works as a PH buffer. Gypsum not so much.
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LocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ



Registered: 04/17/15
Posts: 7,076
Loc: to the brain
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Re: Copelandia cyanescens hyphal knots. [Re: MudaFuka]
#22525768 - 11/14/15 11:55 PM (8 years, 2 months ago) |
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Q&A US vs. THEM The more I learn, the less I know.
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The Dom
Stranger
Registered: 08/28/14
Posts: 30
Last seen: 4 years, 1 month
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Re: Copelandia cyanescens hyphal knots. [Re: LocN9ne]
#22526038 - 11/15/15 01:40 AM (8 years, 2 months ago) |
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So I figured out why I've had such terrible luck with my Pan trops(well I still dont know why it's so slow to germinate) but the plates I had all contammed because I poured them too hot, realized it when my PR and EQ cultures (5/6 of em) got trich right at the edge of the plate(same bag of plates I used when I attempted to grow it on agar last time. So its not my bad luck just my stupidity, luckily this m+m sized growth of P trop is on a plate I poured at another time so it should be just fine til I can get a few plates poured, cant wait to have an abundance of colonized plates(just gives me a feeling of certainty that one way or another I'll find my way to a harvest of them soon, much better than looking at 1cc in a syringe knowing the other 9cc's worth of attempts went )
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LocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ



Registered: 04/17/15
Posts: 7,076
Loc: to the brain
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Re: Copelandia cyanescens hyphal knots. [Re: The Dom]
#22526067 - 11/15/15 02:04 AM (8 years, 2 months ago) |
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I'm not so sure pouring hot is the reason your plates got trich... Some of my plates literally have water I can slosh around in them when I make my mix too lean and they are fine... My bet is your sterile technique is slacking somewhere.
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Q&A US vs. THEM The more I learn, the less I know.
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Copelandia cyanescens hyphal knots. [Re: LocN9ne]
#22526331 - 11/15/15 05:38 AM (8 years, 2 months ago) |
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u still shouldn't pour plates hot though. when u let the agar cool down, its easier to pour because its thicker, and doesn't rush out of the opening like hot agar.
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 3 months
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Re: Copelandia cyanescens hyphal knots. [Re: spacechildo]
#22526370 - 11/15/15 06:25 AM (8 years, 2 months ago) |
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Quote:
spacechildo said: not that much to reserch really, thin subs (~2"), throw some manure or straw in them for better results, up the FAE and temps too, and prepare for lift off! 
Did some research on the effects , I thought you just tripped harder but still had all the confusion but I'm reading pans are clear which is what I'm looking for.
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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Re: Copelandia cyanescens hyphal knots. [Re: taGyo]
#22526524 - 11/15/15 07:51 AM (8 years, 2 months ago) |
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Pan trips are way better than cube trips. They are kind of similar to acid in a way.
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DaveyJones6911
Nonconformist


Registered: 09/08/15
Posts: 690
Loc: EU
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Re: Copelandia cyanescens hyphal knots. [Re: LocN9ne]
#22526622 - 11/15/15 08:28 AM (8 years, 2 months ago) |
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Quote:
LocN9ne said: I'm not so sure pouring hot is the reason your plates got trich... Some of my plates literally have water I can slosh around in them when I make my mix too lean and they are fine... My bet is your sterile technique is slacking somewhere.
what about the pressure changing as it cools down sucking new air into the container?
-------------------- Creativity is a dangerous thing in the eyes of those who don't have any.
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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I always pour my plates hot enough that I burn the shit out of my hand while I pour them. I prefer working with agar when it is still really fluid. I've never had a plate contaminare pre inoculation and I leave uninoculated plates sitting for a verry long time.
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 3 months
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Re: Copelandia cyanescens hyphal knots. [Re: MudaFuka]
#22526734 - 11/15/15 09:06 AM (8 years, 2 months ago) |
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I have un-inoculated plates going on a month with no signs of contam, I actually just did a transfer on to it to see what's up.
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 3 months
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Re: Copelandia cyanescens hyphal knots. [Re: MudaFuka]
#22526753 - 11/15/15 09:10 AM (8 years, 2 months ago) |
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Quote:
MudaFuka said: Pan trips are way better than cube trips. They are kind of similar to acid in a way.
What do you mean by similar to Acid in a mudafuka lay-it-on-me sense , all of the philosophical and spiritual jargon is fucking with the description, is it a cube trip similar to acid or an acid trip? Like am I the universe or am I being guided through the universe?
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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