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OfflinetaGyo
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Re: Copelandia cyanescens hyphal knots. [Re: Kalistis]
    #22523968 - 11/14/15 07:10 PM (8 years, 2 months ago)

So Spacechildo said he exclusively trips on Pans and I did some research and I'm very curious now.

Dropping some on agar tomorrow night :thumbup:


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Offlinespacechildo
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Re: Copelandia cyanescens hyphal knots. [Re: taGyo]
    #22524901 - 11/14/15 09:29 PM (8 years, 2 months ago)

not that much to reserch really, thin subs (~2"), throw some manure or straw in them for better results, up the FAE and temps too, and prepare  for lift off! :hehehe:


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OfflinePsilosoulful

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Re: Copelandia cyanescens hyphal knots. [Re: spacechildo]
    #22525021 - 11/14/15 09:47 PM (8 years, 2 months ago)

:whathesaid:


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OfflinefirstTIMER420
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Re: Copelandia cyanescens hyphal knots. [Re: spacechildo]
    #22525194 - 11/14/15 10:18 PM (8 years, 2 months ago)

Ive always been planning on using half straw half manure, but im thinking about just using strait manure.  Would that be better or worse in yalls opinion?


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OfflinecronicrFacebook
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Re: Copelandia cyanescens hyphal knots. [Re: firstTIMER420]
    #22525206 - 11/14/15 10:20 PM (8 years, 2 months ago)

strait horse manure works great


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OfflinefirstTIMER420
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Re: Copelandia cyanescens hyphal knots. [Re: cronicr]
    #22525212 - 11/14/15 10:21 PM (8 years, 2 months ago)

For some reason when I throw straw in there, it contams. I think because I mess up the moisture content.  Not sure if because last time it was a really weak strain.  But straight horse manure is really easy to get to field capacity with some verm and gypsum in it.


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12 34 http://www.shroomery.org/forums/showflat.php/Number/22386794[/url]
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Edited by firstTIMER420 (11/14/15 10:22 PM)


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OfflinecronicrFacebook
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Re: Copelandia cyanescens hyphal knots. [Re: firstTIMER420]
    #22525231 - 11/14/15 10:24 PM (8 years, 2 months ago)

try add lime next time you use straw


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InvisibleLocN9ne
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Re: Copelandia cyanescens hyphal knots. [Re: cronicr]
    #22525288 - 11/14/15 10:32 PM (8 years, 2 months ago)

Quote:

cronicr said:
try add lime next time you use straw



What difference does the lime cause as opposed to gypsum?


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OfflinecronicrFacebook
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Re: Copelandia cyanescens hyphal knots. [Re: LocN9ne]
    #22525325 - 11/14/15 10:39 PM (8 years, 2 months ago)

lime raises the ph, gypsum stablizes the ph and prevents it from swinging, as it colonizes it becomes more acidic causing the ph to drop, gypsum helps this


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InvisibleMudaFuka
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Re: Copelandia cyanescens hyphal knots. [Re: LocN9ne]
    #22525336 - 11/14/15 10:41 PM (8 years, 2 months ago)

Lime works as a PH buffer. Gypsum not so much.


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InvisibleLocN9ne
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Re: Copelandia cyanescens hyphal knots. [Re: MudaFuka]
    #22525768 - 11/14/15 11:55 PM (8 years, 2 months ago)

:takingnotes:


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OfflineThe Dom
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Re: Copelandia cyanescens hyphal knots. [Re: LocN9ne]
    #22526038 - 11/15/15 01:40 AM (8 years, 2 months ago)

So I figured out why I've had such terrible luck with my Pan trops(well I still dont know why it's so slow to germinate) but the plates I had all contammed because I poured them too hot, realized it when my PR and EQ cultures (5/6 of em) got trich right at the edge of the plate(same bag of plates I used when I attempted to grow it on agar last time. So its not my bad luck just my stupidity, luckily this m+m sized growth of P trop is on a plate I poured at another time so it should be just fine til I can get a few plates poured, cant wait to have an abundance of colonized plates(just gives me a feeling of certainty that one way or another I'll find my way to a harvest of them soon, much better than looking at 1cc in a syringe knowing the other 9cc's worth of attempts went  :poopbanana: )


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InvisibleLocN9ne
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Re: Copelandia cyanescens hyphal knots. [Re: The Dom]
    #22526067 - 11/15/15 02:04 AM (8 years, 2 months ago)

I'm not so sure pouring hot is the reason your plates got trich... Some of my plates literally have water I can slosh around in them when I make my mix too lean and they are fine... My bet is your sterile technique is slacking somewhere.


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Invisibleblindingleaf
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Re: Copelandia cyanescens hyphal knots. [Re: LocN9ne]
    #22526331 - 11/15/15 05:38 AM (8 years, 2 months ago)

:whathesaid:

u still shouldn't pour plates hot though.  when u let the agar cool down, its easier to pour because its thicker, and doesn't rush out of the opening like hot agar.


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OfflinetaGyo
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Re: Copelandia cyanescens hyphal knots. [Re: spacechildo]
    #22526370 - 11/15/15 06:25 AM (8 years, 2 months ago)

Quote:

spacechildo said:
not that much to reserch really, thin subs (~2"), throw some manure or straw in them for better results, up the FAE and temps too, and prepare  for lift off! :hehehe:



Did some research on the effects :lol:, I thought you just tripped harder but still had all the confusion but I'm reading pans are clear which is what I'm looking for.


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InvisibleMudaFuka
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Re: Copelandia cyanescens hyphal knots. [Re: taGyo]
    #22526524 - 11/15/15 07:51 AM (8 years, 2 months ago)

Pan trips are way better than cube trips. They are kind of similar to acid in a way.


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InvisibleDaveyJones6911
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Re: Copelandia cyanescens hyphal knots. [Re: LocN9ne]
    #22526622 - 11/15/15 08:28 AM (8 years, 2 months ago)

Quote:

LocN9ne said:
I'm not so sure pouring hot is the reason your plates got trich... Some of my plates literally have water I can slosh around in them when I make my mix too lean and they are fine... My bet is your sterile technique is slacking somewhere.




what about the pressure changing as it cools down sucking new air into the container?


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InvisibleMudaFuka
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Re: Copelandia cyanescens hyphal knots. [Re: DaveyJones6911]
    #22526641 - 11/15/15 08:35 AM (8 years, 2 months ago)

I always pour my plates hot enough that I burn the shit out of  my hand while I pour them. I prefer working with agar when it is still really fluid. I've never had a plate contaminare pre inoculation and I leave uninoculated plates sitting for a verry long time.


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OfflinetaGyo
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Re: Copelandia cyanescens hyphal knots. [Re: MudaFuka]
    #22526734 - 11/15/15 09:06 AM (8 years, 2 months ago)

I have un-inoculated plates going on a month with no signs of contam, I actually just did a transfer on to it to see what's up.


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OfflinetaGyo
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Re: Copelandia cyanescens hyphal knots. [Re: MudaFuka]
    #22526753 - 11/15/15 09:10 AM (8 years, 2 months ago)

Quote:

MudaFuka said:
Pan trips are way better than cube trips. They are kind of similar to acid in a way.



What do you mean by similar to Acid in a mudafuka lay-it-on-me sense :lol:, all of the philosophical and spiritual jargon is fucking with the description, is it a cube trip similar to acid or an acid trip? Like am I the universe or am I being guided through the universe?


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