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Invisiblenube424
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Re: Official Copelandia/Panaeolus Thread [Re: mushboy]
    #25741334 - 01/13/19 06:22 AM (5 years, 17 days ago)

Yea I think he means lc.

So, my bisporus mycelium looked like the ones in his pics on agar. But now that It colonized my grain jars, it seriously looks like mold. But I can still see the strands on mycelium growing over the grains. So, I'm HOPING I have jars full of mycelium and not mold. Shit looks weird tho :facepalm3:


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OfflineGeinstein Happy Birthday!
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Re: Official Copelandia/Panaeolus Thread [Re: Brain Bulb]
    #25741494 - 01/13/19 08:19 AM (5 years, 17 days ago)

Quote:

Brain Bulb said:
Well wouldn’t you know it.  Maybe a couple days after my last post my Pans got hit by the green monster...

Already have the humidifiers and am about to buy a small GH.  Going to get these to fruit come hell or high water!  My LI tested clean and it’s thick with myc now so time to inoculate some hpoo, straw and wbs.



Also think I have a new phenotype I transferred from my LI test plate so here we go.




Most of my work is ever agar dropped to grain or LI with agar
With LI you drop agar to water shake and knock up your jars as soon as possible
That don't look like LI
It can be that you used a lot of agar for a strong LI and the mycelium is eating the broken up agar. . .and that's the colour or something. . .

But I really think you just made a LC:shrug:

But then again LC can be seen as a form of LI?:shrug:
So good for you bro!
Beput then what do I know:lol:


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Nothing breads nothing


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OfflineJakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: Geinstein]
    #25741613 - 01/13/19 09:21 AM (5 years, 17 days ago)

Getting closer to my pan mono tub canopy


--------------------
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InvisibleHobbyist
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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
    #25741655 - 01/13/19 09:44 AM (5 years, 17 days ago)

What are your guys' opinions on strawnet? 

A straw substrate you don't have to pasteurize?  Seems ideal...


--------------------
Everything i say is completely hypothetical...



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OfflineBrain Bulb
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Re: Official Copelandia/Panaeolus Thread [Re: Hobbyist]
    #25742039 - 01/13/19 01:29 PM (5 years, 17 days ago)

Yep, looks like LC is the correct term.  Sorry.  I didn’t use an agar wedge though.  Used sterile swabs to collect myc from a plate and stirred it into the grain soak/LME water.  Used some of it already but it’s been growing since 12/05.

Already had a 12cc syringe loaded and sterile jar of grain so I inoculated it on 1/11.  Starting to see myc!  Going with a glass tray this time for sure.


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Edited by Brain Bulb (01/13/19 01:30 PM)


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OfflineBrain Bulb
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Re: Official Copelandia/Panaeolus Thread [Re: Asura]
    #25742046 - 01/13/19 01:32 PM (5 years, 17 days ago)

Quote:

Asura said:
Yeah Brain, that looks solid. That is the phenotype you want.





Thanks Asura!


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Offlinemullugh
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Re: Official Copelandia/Panaeolus Thread [Re: Brain Bulb]
    #25742958 - 01/14/19 04:22 AM (5 years, 16 days ago)

Although I said I was shelving this until summer, after tripping on pans the other night, I've changed my mind again. So I've modified my shoe boxes with holes around surface level to just allow a bit more stir in (I always felt stale air was an issue) ad's I might rig up humidifier and fan too to get everything hunky dory.


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Offlinemullugh
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Re: Official Copelandia/Panaeolus Thread [Re: mullugh]
    #25742960 - 01/14/19 04:27 AM (5 years, 16 days ago)

I was several hours into a cube trip, took a load of pans and was fucked in a good way


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InvisibleCaps McGee
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Re: Official Copelandia/Panaeolus Thread [Re: mullugh]
    #25743033 - 01/14/19 05:43 AM (5 years, 16 days ago)

Posting for future relation


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OfflineShaperDreaming
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Re: Official Copelandia/Panaeolus Thread [Re: Caps McGee]
    #25743247 - 01/14/19 09:11 AM (5 years, 16 days ago)

So my Pan Trop agar plate still isn't looking good. No growth at all for anything I'd be confident calling mycelium. I mixed up a different agar recipe (switching grain water for LME), and I'm going to use an inoculation loop to try to get another plate noc'ed up tomorrow night. Fingers crossed I grow more than bacteria this time.


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Invisiblenube424
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Re: Official Copelandia/Panaeolus Thread [Re: ShaperDreaming]
    #25743256 - 01/14/19 09:16 AM (5 years, 16 days ago)

Ayyyy caps, good to see u here. U got this man.


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OfflineJakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: ShaperDreaming]
    #25743505 - 01/14/19 12:01 PM (5 years, 16 days ago)

Quote:

ShaperDreaming said:
So my Pan Trop agar plate still isn't looking good. No growth at all for anything I'd be confident calling mycelium. I mixed up a different agar recipe (switching grain water for LME), and I'm going to use an inoculation loop to try to get another plate noc'ed up tomorrow night. Fingers crossed I grow more than bacteria this time.



I have found that pan myc really likes peptone agar


--------------------
Natural omt/detox
online  pant cult classes available last Saturday of every month go to buymeacoffee.com/jakeoncid to sign up (1 on 1 consultations also available
JOC PAN TEK
CORDYCEPS MILITARIS

EXOTICS
[


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OfflineShaperDreaming
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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
    #25743516 - 01/14/19 12:07 PM (5 years, 16 days ago)

Quote:

Jakeoncid419 said:
I have found that pan myc really likes peptone agar



I searched a little bit for what peptone is, but I'm unfamiliar with what I'm looking for. Would something labeled "Silk Peptide Powder" work as peptone? Also, how much are you using in your recipes?


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InvisibleCaps McGee
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Re: Official Copelandia/Panaeolus Thread [Re: ShaperDreaming]
    #25743551 - 01/14/19 12:25 PM (5 years, 16 days ago)

:rockon::rockon::rockon:


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OfflineJakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: ShaperDreaming]
    #25743553 - 01/14/19 12:27 PM (5 years, 16 days ago)

Quote:

ShaperDreaming said:
Quote:

Jakeoncid419 said:
I have found that pan myc really likes peptone agar



I searched a little bit for what peptone is, but I'm unfamiliar with what I'm looking for. Would something labeled "Silk Peptide Powder" work as peptone? Also, how much are you using in your recipes?



u will find peptone on most myco supply sites. I have a few different recipes the what ive been using to make pan inoculation dishes is
1000ml water
20gs agar
15gs malt
7gs peptone
.05 gentamicin sulfate antibiotic powder

for storage I run
1000ml water
20g agar
30gs BRF
5gs malt
3gs peptone
.05 GSAP
and just so you know that pans are always throwing curve balls, spotted this green mold in my brand new trop tray :frown: never even got to the surface. im gonna have to check out my trop spawn. I got it in a ss put it to agar (8 months ago) and it has been on grain or agar ever since the culture dwindled all the way down to one jar at which point I dropped colonized grain on agar to bring it back, but some jars have been late contaming after looking perfect for weeks. this spawn looked good but this tray triched out fast AF. that's the fun of pans tho always kept on ur toes always something to do. it can be frustrating but so much more rewarding when you succeed


--------------------
Natural omt/detox
online  pant cult classes available last Saturday of every month go to buymeacoffee.com/jakeoncid to sign up (1 on 1 consultations also available
JOC PAN TEK
CORDYCEPS MILITARIS

EXOTICS
[


Edited by Jakeoncid419 (01/14/19 12:29 PM)


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OfflineBrain Bulb
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Re: Official Copelandia/Panaeolus Thread [Re: ShaperDreaming] * 1
    #25743595 - 01/14/19 12:50 PM (5 years, 16 days ago)

T3 Pan. Bisporus coming along nicely!



And that’s not mold in the second pic.  I dropped the transfer upside down and flipped it real quick.


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Invisiblenube424
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Re: Official Copelandia/Panaeolus Thread [Re: Brain Bulb]
    #25743616 - 01/14/19 01:00 PM (5 years, 16 days ago)

Wait till u put that shit on grain, it looks soooo weird.


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OfflineNorfolk243

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Re: Official Copelandia/Panaeolus Thread [Re: Brain Bulb]
    #25743922 - 01/14/19 03:48 PM (5 years, 15 days ago)

pan cam goliath jars fully colonized. Will be making a tray with this tomorrow, and create a suitable FC to fruit them in. my house is around 30-40% RH. my SGFC isnt going to suffice. im having a hard time getting it above 85% RH inside my sgfc.  i may try to create a HC with a humidifier rigged to it to have the correct air exchange and humidity as i dont have the space for a GH.


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OfflineJakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: Norfolk243]
    #25743940 - 01/14/19 03:54 PM (5 years, 15 days ago)

Quote:

Norfolk243 said:
pan cam goliath jars fully colonized. Will be making a tray with this tomorrow, and create a suitable FC to fruit them in. my house is around 30-40% RH. my SGFC isnt going to suffice. im having a hard time getting it above 85% RH inside my sgfc.  i may try to create a HC with a humidifier rigged to it to have the correct air exchange and humidity as i dont have the space for a GH.



check out my mono tub mod I have my fae cranked and my sub doesn't dry out. ive been having a harder time with the trays but I think I got em dialed in now, still get more from the tubs tho


--------------------
Natural omt/detox
online  pant cult classes available last Saturday of every month go to buymeacoffee.com/jakeoncid to sign up (1 on 1 consultations also available
JOC PAN TEK
CORDYCEPS MILITARIS

EXOTICS
[


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OfflinePsilosopherr
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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
    #25743965 - 01/14/19 04:05 PM (5 years, 15 days ago)

:lol: coke bottle.

first time i saw your tubs with piping it seemed silly to me but I kinda like the idea now


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