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mary fairchild
Pantheist


Registered: 12/06/14
Posts: 777
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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
#25737119 - 01/10/19 10:02 PM (5 years, 19 days ago) |
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Yes-I did not fruit it in the bag.
I broke out the colonized cake in the bag then laid it into a tray- give the surface a sprits with some water and put it directly into the greenhouse- with fruiting atmospheric conditions.
So- it would recover in ~2-3 days(meaning the surface of the sub would colonize). At that point I would case it with 50/50 +-> put it back into the GH and wait for fruiting.
Regarding my earlier experiences with tropicalis- for me it didn't work in the LC-> sterilized sub. bag procedure, when I get around to it i'm going to do Spawn to Bulk protocol instead and maybe PF tek..
Right now i'm am totally spent from this trip- so I'm signing off for the night.
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Jakeoncid419
Follow me.....



Registered: 06/26/18
Posts: 4,390
Loc: Basel Switzerland
Last seen: 1 day, 17 hours
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Quote:
mary fairchild said: Yes-I did not fruit it in the bag.
I broke out the colonized cake in the bag then laid it into a tray- give the surface a sprits with some water and put it directly into the greenhouse- with fruiting atmospheric conditions.
So- it would recover in ~2-3 days(meaning the surface of the sub would colonize). At that point I would case it with 50/50 +-> put it back into the GH and wait for fruiting.
Regarding my earlier experiences with tropicalis- for me it didn't work in the LC-> sterilized sub. bag procedure, when I get around to it i'm going to do Spawn to Bulk protocol instead and maybe PF tek..
Right now i'm am totally spent from this trip- so I'm signing off for the night.
Lol right on I just ate a pan/tamp chocolate so I’m taking off got the hobbit trilogy cued up haha
-------------------- Natural omt/detox online pant cult classes available last Saturday of every month go to buymeacoffee.com/jakeoncid to sign up (1 on 1 consultations also available JOC PAN TEK CORDYCEPS MILITARIS EXOTICS [
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bug_handler
Stranger
Registered: 12/04/18
Posts: 3
Last seen: 4 years, 9 months
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Re: Official Copelandia/Panaeolus Thread [Re: bug_handler]
#25737541 - 01/11/19 05:57 AM (5 years, 19 days ago) |
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Quote:
bug_handler said: Greetings. I've just started using agar and make my first mistakes (inoculating with syringe instead of just transferring spores, thus creating excess water in the plate). So, three plates which have been inoculated with Panaeolus tropicalis syringe now have some signs of life. I need your help identifying it. It seems like young Aspergillus flavus colonies to me. I wish I'm wrong.
 
Just a little update on my "work". With true colours you may see that it's definitely not Pan. mycelium.
 Same time two other plates, which have had no mycelium, now have something. I will try to make some pics of them, just to compare.
Edited by bug_handler (01/11/19 05:58 AM)
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nube424


Registered: 12/03/17
Posts: 6,063
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Re: Official Copelandia/Panaeolus Thread [Re: J. Jack Flash]
#25737553 - 01/11/19 06:08 AM (5 years, 19 days ago) |
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Quote:
Asura said: I mean if you want to do things in the most expensive possible way, Tums would work. Need like 10 packs of them.
Quote:
J. Jack Flash said: you eat a lot of eggs, nube? eggshells are CaCO3
That's dudes, that's pretty much exactly what I wanted to know
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rickyswamps
Bad Apple



Registered: 11/08/18
Posts: 1,192
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Re: Official Copelandia/Panaeolus Thread [Re: bug_handler]
#25737565 - 01/11/19 06:18 AM (5 years, 19 days ago) |
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Quote:
bug_handler said:
Quote:
bug_handler said: Greetings. I've just started using agar and make my first mistakes (inoculating with syringe instead of just transferring spores, thus creating excess water in the plate). So, three plates which have been inoculated with Panaeolus tropicalis syringe now have some signs of life. I need your help identifying it. It seems like young Aspergillus flavus colonies to me. I wish I'm wrong.
 
Just a little update on my "work". With true colours you may see that it's definitely not Pan. mycelium.
 Same time two other plates, which have had no mycelium, now have something. I will try to make some pics of them, just to compare.
Is that a vendor syringe? I haven't seen that kind of coloring before, but whatever you have, it looks clean. Maybe someone can help you figure it out. Its ready to transfer btw, if you plan on keeping it.
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nube424


Registered: 12/03/17
Posts: 6,063
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Re: Official Copelandia/Panaeolus Thread [Re: rickyswamps]
#25737586 - 01/11/19 06:32 AM (5 years, 19 days ago) |
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Finally got enough colonization to snap a pic. Is this on par with pan bisp mycelium? 
All the black specs are nijer seed. I didn't realize this brand of wbs had so much of it till It was too late.
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bug_handler
Stranger
Registered: 12/04/18
Posts: 3
Last seen: 4 years, 9 months
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Re: Official Copelandia/Panaeolus Thread [Re: rickyswamps]
#25737590 - 01/11/19 06:35 AM (5 years, 19 days ago) |
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No, at first it was a spore print. Then I made a syringe to rehydrate spores (now I think this was a wrong choice. I'd better used a loop and directly passed the spore to agar). One more thing to mention. It was Panaeolus cambodginiensis spore print.
Edited by bug_handler (01/11/19 06:37 AM)
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nube424


Registered: 12/03/17
Posts: 6,063
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Re: Official Copelandia/Panaeolus Thread [Re: bug_handler]
#25737599 - 01/11/19 06:39 AM (5 years, 19 days ago) |
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Yes, try making ur agar a little bit softer than normal and swipe right onto the agar.
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ShaperDreaming
Weirdo



Registered: 10/30/18
Posts: 3,429
Loc: United States
Last seen: 2 years, 3 days
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Quote:
mary fairchild said:I just ordered a nice pH meter, Apera Inst. it has a neat probe that you just stick into whatever you are sampling, so you dont have to make a slurry. - so you can just stick it into the substrate, or case- as long as its hydrated. I guess its pricey by our standards but my current probe not trustworthy.
So I have one of these things for my succulents, but I've never used the pH meter for it. Would something like this be worth testing?
Which leads me to the next question... What am I paying attention to pH for in all this? Where is it useful?
These Pans seem like a huge pan in ass.
I'm starting to realize I may need to construct a custom rig for this grow. I have some neat fun ideas going through my head (I do like to build shit), so I'm glad I have some time while this shit germinates on agar.
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zxcv101
Stranger


Registered: 07/09/09
Posts: 64
Loc: SW
Last seen: 4 years, 22 days
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This doesn't look right. The mycelium colonized most of the way through my 1/4 inch casing layer. I'm seeing lots of metabolites and I believe this is aerial mycelium. I'm assuming I've got FAE problems?



My casing was 50/50 peat verm. Cased 5 days ago.
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Johnny Dont
500 Don'ts of Knife Safety


Registered: 04/24/14
Posts: 1,830
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Re: Official Copelandia/Panaeolus Thread [Re: zxcv101]
#25738361 - 01/11/19 02:14 PM (5 years, 19 days ago) |
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So I think I finally have some clean cultures. This one looks the best




I've been tryn to isolate away from some sorta contaminate that sorta looks matted once its grown out a bit. You can see it from about 6oclock to 9

Sense all of these are 1 transfer away from contaminanted dishes, I should do another round of transfers right? Or could I put the first dish to grains? And just do transfers on the other 3?
-------------------- I have left life and loves behind me, to be blown about as the sea desires, to have the freedom of the open air, and to be witness to the making of the world.
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Jakeoncid419
Follow me.....



Registered: 06/26/18
Posts: 4,390
Loc: Basel Switzerland
Last seen: 1 day, 17 hours
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Re: Official Copelandia/Panaeolus Thread [Re: Asura]
#25738401 - 01/11/19 02:37 PM (5 years, 19 days ago) |
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Quote:
Asura said: Cranberry juice + 2g dried pans. Very simple formula to find the One Mind.
Come on, Jake...do it!
I’ll def give it a shot I ate about 4 gs in chocolate last night then while tripping wondered into my grow room and ate a few freshies our the tray lol I was out there but still came back 7 hours later I love how clean pans are, they don’t give me a mind fuck even at high doses, super intense visuals very giggly love em
-------------------- Natural omt/detox online pant cult classes available last Saturday of every month go to buymeacoffee.com/jakeoncid to sign up (1 on 1 consultations also available JOC PAN TEK CORDYCEPS MILITARIS EXOTICS [
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J. Jack Flash
stranger than ever.

Registered: 11/20/13
Posts: 1,500
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Quote:
ShaperDreaming said:
Quote:
mary fairchild said:I just ordered a nice pH meter, Apera Inst. it has a neat probe that you just stick into whatever you are sampling, so you dont have to make a slurry. - so you can just stick it into the substrate, or case- as long as its hydrated. I guess its pricey by our standards but my current probe not trustworthy.
So I have one of these things for my succulents, but I've never used the pH meter for it. Would something like this be worth testing?
Which leads me to the next question... What am I paying attention to pH for in all this? Where is it useful?
These Pans seem like a huge pan in ass.
I'm starting to realize I may need to construct a custom rig for this grow. I have some neat fun ideas going through my head (I do like to build shit), so I'm glad I have some time while this shit germinates on agar.
this is my understanding. anyone who knows better, please correct me.
pan cyans, at least, require a casing to fruit. peat is often used as casing material. peat is acidic, which is bad for casing. so a pH buffer such as garden lime is added to raise the pH to just above neutral. (weakly alkaline) a pH tester is a means to decide how much of the buffer to add. i've opted to use test strips. i believe i read that mary fairchild found the strips not to be very reliable, tho.
garden lime is calcium carbonate. CaCO3. eggshells are also almost entirely CaCO3. as an observational experiment, i used powdered eggshells to buffer some jiffy mix (mostly peat) to use for a tiny cube project i have underway. test strips indicated success, and that project is pinning nicely right now. (i know, cubes don't need casing, but i want the experience)
relatedly: someone mentioned using baking soda, but that's trickier because of a quirk of carbonate chemistry. CaCO3 dissolves poorly in water, but its solubility improves with increasing acidity. so an acidic solution dissolves enough of it to return it to a weakly alkaline state, at which point it stops dissolving, kind of automagically limiting pH increase, making it more forgiving of adding too much. baking soda, by comparison, is more soluble in water, thus much easier to raise pH too high.
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the j stands for jesus.2020 new years grow along
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nube424


Registered: 12/03/17
Posts: 6,063
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Re: Official Copelandia/Panaeolus Thread [Re: nube424]
#25738426 - 01/11/19 02:54 PM (5 years, 19 days ago) |
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Quote:
nube424 said: Finally got enough colonization to snap a pic. Is this on par with pan bisp mycelium? 
All the black specs are nijer seed. I didn't realize this brand of wbs had so much of it till It was too late. 
Bump? I'm really curious and concerned about how this mycelium looks. Any thoughts?
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ShaperDreaming
Weirdo



Registered: 10/30/18
Posts: 3,429
Loc: United States
Last seen: 2 years, 3 days
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Re: Official Copelandia/Panaeolus Thread [Re: J. Jack Flash]
#25738441 - 01/11/19 02:58 PM (5 years, 19 days ago) |
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Thanks J.J! That makes sense. When you make/mix this casing do you do these measurements before pasteurization or after? Also, what's the pasteurization process for these items?
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Jakeoncid419
Follow me.....



Registered: 06/26/18
Posts: 4,390
Loc: Basel Switzerland
Last seen: 1 day, 17 hours
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Re: Official Copelandia/Panaeolus Thread [Re: J. Jack Flash]
#25738444 - 01/11/19 02:58 PM (5 years, 19 days ago) |
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Quote:
J. Jack Flash said:
Quote:
ShaperDreaming said:
Quote:
mary fairchild said:I just ordered a nice pH meter, Apera Inst. it has a neat probe that you just stick into whatever you are sampling, so you dont have to make a slurry. - so you can just stick it into the substrate, or case- as long as its hydrated. I guess its pricey by our standards but my current probe not trustworthy.
So I have one of these things for my succulents, but I've never used the pH meter for it. Would something like this be worth testing?
Which leads me to the next question... What am I paying attention to pH for in all this? Where is it useful?
These Pans seem like a huge pan in ass.
I'm starting to realize I may need to construct a custom rig for this grow. I have some neat fun ideas going through my head (I do like to build shit), so I'm glad I have some time while this shit germinates on agar.
this is my understanding. anyone who knows better, please correct me.
pan cyans, at least, require a casing to fruit. peat is often used as casing material. peat is acidic, which is bad for casing. so a pH buffer such as garden lime is added to raise the pH to just above neutral. (weakly alkaline) a pH tester is a means to decide how much of the buffer to add. i've opted to use test strips. i believe i read that mary fairchild found the strips not to be very reliable, tho.
garden lime is calcium carbonate. CaCO3. eggshells are also almost entirely CaCO3. as an observational experiment, i used powdered eggshells to buffer some jiffy mix (mostly peat) to use for a tiny cube project i have underway. test strips indicated success, and that project is pinning nicely right now. (i know, cubes don't need casing, but i want the experience)
relatedly: someone mentioned using baking soda, but that's trickier because of a quirk of carbonate chemistry. CaCO3 dissolves poorly in water, but its solubility improves with increasing acidity. so an acidic solution dissolves enough of it to return it to a weakly alkaline state, at which point it stops dissolving, kind of automagically limiting pH increase, making it more forgiving of adding too much. baking soda, by comparison, is more soluble in water, thus much easier to raise pH too high.
exactly and the casing's thickness is super important it must be kept thin! Ive been using peat and verm at 60/40 peat heavy balanced with hydrated lime (I use a analog ph meter probably not exactly accurate but as long as ur between 7-7.8 you will be fine. I also throw in a handful of cos and ive been noticing that a lot of the mushroom pop up under or near pieces of cos (idk if this is because the cos does not get colonized or what. (however ive ran tubs with no cos and been fine) I used to cut potassium sulfate into my spawn as wll as a few other added chems along they way and eventually I will start trying them to see if I notice an improvement but as it is now I don't even add gypsum just spawn poo straw and verm cased with above mentioned
-------------------- Natural omt/detox online pant cult classes available last Saturday of every month go to buymeacoffee.com/jakeoncid to sign up (1 on 1 consultations also available JOC PAN TEK CORDYCEPS MILITARIS EXOTICS [
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ShaperDreaming
Weirdo



Registered: 10/30/18
Posts: 3,429
Loc: United States
Last seen: 2 years, 3 days
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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
#25738466 - 01/11/19 03:10 PM (5 years, 19 days ago) |
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If something is labeled "Food grade calcium hydroxide with no additives or preservatives" AKA "Food grade lime" ... can that be used in place of CaCO3 (why do I have a feeling this is a dumb question).
Edited by ShaperDreaming (01/11/19 03:11 PM)
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J. Jack Flash
stranger than ever.

Registered: 11/20/13
Posts: 1,500
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no way. hydroxides are extremely alkaline. you'll make soap before mushrooms. you'll also suffer chemical burns. so no, not a dumb question.
--------------------
the j stands for jesus.2020 new years grow along
Edited by J. Jack Flash (01/11/19 03:25 PM)
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ShaperDreaming
Weirdo



Registered: 10/30/18
Posts: 3,429
Loc: United States
Last seen: 2 years, 3 days
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Re: Official Copelandia/Panaeolus Thread [Re: J. Jack Flash]
#25738488 - 01/11/19 03:25 PM (5 years, 19 days ago) |
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Quote:
J. Jack Flash said: no way. hydroxides are extremely alkaline. you'll make soap before mushrooms. you'll also suffer chemical burns.
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Jakeoncid419
Follow me.....



Registered: 06/26/18
Posts: 4,390
Loc: Basel Switzerland
Last seen: 1 day, 17 hours
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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
#25738512 - 01/11/19 03:37 PM (5 years, 19 days ago) |
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Ok so hydrated lime is really only used ever by small hobbie (usually active growers) under most other circumstances Hydrated lime should not (and is not) be used. Calcium carbonate is better safer and cheaper. depending on the grade of ur HL and how much u use you can easily fuck up ur shit. Some grades of hl are more caustic than others so like a box of chocolates..... You can get Calcium carbonate powder In a few different forms. some garden stores usually call it garden lime, “ground oyster shells”, limestone, “any form of calcium-carbonate-based lime”. You do not want dolomite-base lime (magnesium-rich form). Ive also read that you can get it from wine making shops. If you already have HL and ur nervous about using it spread it about ½ inch thick on the bottom of a glass pan (NOT metal) cook at 350F for a hour. heat and CO2 in the air will covert most Of ur hL to calcium carbonate.
-------------------- Natural omt/detox online pant cult classes available last Saturday of every month go to buymeacoffee.com/jakeoncid to sign up (1 on 1 consultations also available JOC PAN TEK CORDYCEPS MILITARIS EXOTICS [
Edited by Jakeoncid419 (01/11/19 03:39 PM)
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