Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Bridgetown Botanicals CBD Concentrates   Kraken Kratom Red Vein Kratom   Original Sensible Seeds Autoflowering Cannabis Seeds   PhytoExtractum Kratom Powder for Sale   Left Coast Kratom Buy Kratom Capsules   North Spore Bulk Substrate   Mushroom-Hut Liquid Cultures

Jump to first unread post Pages: < First | < Back | 356 | 357 | 358 | 359 | 360 | 361 | 362 | 363 | 364 | 365 | 366 | 367 | 368 | 369 | 370 | 371 | 372 | 373 | 374 | 375 | 376 | Next > | Last >
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Invisiblemary fairchild
Pantheist


Registered: 12/06/14
Posts: 777
Trusted Cultivator
Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
    #25737119 - 01/10/19 10:02 PM (5 years, 19 days ago)

Yes-I did not fruit it in the bag.

I broke out the colonized cake in the bag then laid it into a tray- give the surface a sprits with some water and put it directly into the greenhouse- with fruiting atmospheric conditions.

So- it would recover in ~2-3 days(meaning the surface of the sub would colonize).
At that point I  would case it with 50/50 +-> put it back into the GH and wait for fruiting.

Regarding my earlier experiences with tropicalis- for me it didn't work in the LC-> sterilized sub. bag procedure, when I get around to it i'm going to do Spawn to Bulk protocol instead and maybe PF tek..

Right now i'm am totally spent from this trip- so I'm signing off for the night.


Extras: Filter Print Post Top
OfflineJakeoncid419
Follow me.....
Male User Gallery


Registered: 06/26/18
Posts: 4,390
Loc: Basel Switzerland
Last seen: 1 day, 17 hours
Trusted Cultivator
Re: Official Copelandia/Panaeolus Thread [Re: mary fairchild]
    #25737126 - 01/10/19 10:04 PM (5 years, 19 days ago)

Quote:

mary fairchild said:
Yes-I did not fruit it in the bag.

I broke out the colonized cake in the bag then laid it into a tray- give the surface a sprits with some water and put it directly into the greenhouse- with fruiting atmospheric conditions.

So- it would recover in ~2-3 days(meaning the surface of the sub would colonize).
At that point I  would case it with 50/50 +-> put it back into the GH and wait for fruiting.

Regarding my earlier experiences with tropicalis- for me it didn't work in the LC-> sterilized sub. bag procedure, when I get around to it i'm going to do Spawn to Bulk protocol instead and maybe PF tek..

Right now i'm am totally spent from this trip- so I'm signing off for the night.



Lol right on I just ate a pan/tamp chocolate so I’m taking off got the hobbit trilogy cued up haha


--------------------
Natural omt/detox
online  pant cult classes available last Saturday of every month go to buymeacoffee.com/jakeoncid to sign up (1 on 1 consultations also available
JOC PAN TEK
CORDYCEPS MILITARIS

EXOTICS
[


Extras: Filter Print Post Top
Offlinebug_handler
Stranger
Registered: 12/04/18
Posts: 3
Last seen: 4 years, 9 months
Re: Official Copelandia/Panaeolus Thread [Re: bug_handler]
    #25737541 - 01/11/19 05:57 AM (5 years, 19 days ago)

Quote:

bug_handler said:
Greetings. I've just started using agar and make my first mistakes (inoculating with syringe instead of just transferring spores, thus creating excess water in the plate). So, three plates which have been inoculated with Panaeolus tropicalis syringe now have some signs of life. I need your help  identifying it. It seems like young Aspergillus flavus colonies to me. I wish I'm wrong.




Just a little update on my "work". With true colours you may see that it's definitely not Pan. mycelium.

Same time two other plates, which have had no mycelium, now have something. I will try to make some pics of them, just to compare.


Edited by bug_handler (01/11/19 05:58 AM)


Extras: Filter Print Post Top
Invisiblenube424
Male

Registered: 12/03/17
Posts: 6,063
Re: Official Copelandia/Panaeolus Thread [Re: J. Jack Flash]
    #25737553 - 01/11/19 06:08 AM (5 years, 19 days ago)

Quote:

Asura said:
I mean if you want to do things in the most expensive possible way, Tums would work. Need like 10 packs of them.



Quote:

J. Jack Flash said:
you eat a lot of eggs, nube? eggshells are CaCO3



That's dudes, that's pretty much exactly what I wanted to know :thumbup:


Extras: Filter Print Post Top
Invisiblerickyswamps
Bad Apple
I'm a teapot


Registered: 11/08/18
Posts: 1,192
Re: Official Copelandia/Panaeolus Thread [Re: bug_handler]
    #25737565 - 01/11/19 06:18 AM (5 years, 19 days ago)

Quote:

bug_handler said:
Quote:

bug_handler said:
Greetings. I've just started using agar and make my first mistakes (inoculating with syringe instead of just transferring spores, thus creating excess water in the plate). So, three plates which have been inoculated with Panaeolus tropicalis syringe now have some signs of life. I need your help  identifying it. It seems like young Aspergillus flavus colonies to me. I wish I'm wrong.




Just a little update on my "work". With true colours you may see that it's definitely not Pan. mycelium.

Same time two other plates, which have had no mycelium, now have something. I will try to make some pics of them, just to compare.




Is that a vendor syringe?  I haven't seen that kind of coloring before, but whatever you have, it looks clean.  Maybe someone can help you figure it out.  Its ready to transfer btw, if you plan on keeping it.


Extras: Filter Print Post Top
Invisiblenube424
Male

Registered: 12/03/17
Posts: 6,063
Re: Official Copelandia/Panaeolus Thread [Re: rickyswamps]
    #25737586 - 01/11/19 06:32 AM (5 years, 19 days ago)

Finally got enough colonization to snap a pic. Is this on par with pan bisp mycelium?


All the black specs are nijer seed. I didn't realize this brand of wbs had so much of it till It was too late. :facepalm3:


Extras: Filter Print Post Top
Offlinebug_handler
Stranger
Registered: 12/04/18
Posts: 3
Last seen: 4 years, 9 months
Re: Official Copelandia/Panaeolus Thread [Re: rickyswamps]
    #25737590 - 01/11/19 06:35 AM (5 years, 19 days ago)

No, at first it was a spore print. Then I made a syringe to rehydrate spores (now I think this was a wrong choice. I'd better used a loop and directly passed the spore to agar).
One more thing to mention. It was Panaeolus cambodginiensis spore print.


Edited by bug_handler (01/11/19 06:37 AM)


Extras: Filter Print Post Top
Invisiblenube424
Male

Registered: 12/03/17
Posts: 6,063
Re: Official Copelandia/Panaeolus Thread [Re: bug_handler]
    #25737599 - 01/11/19 06:39 AM (5 years, 19 days ago)

Yes, try making ur agar a little  bit softer than normal and swipe right onto the agar.


Extras: Filter Print Post Top
OfflineShaperDreaming
Weirdo
Female User Gallery


Registered: 10/30/18
Posts: 3,429
Loc: United States Flag
Last seen: 2 years, 3 days
Re: Official Copelandia/Panaeolus Thread [Re: mary fairchild]
    #25737932 - 01/11/19 09:55 AM (5 years, 19 days ago)

Quote:

mary fairchild said:I just ordered a nice pH meter, Apera Inst. it has a neat probe that you just stick into whatever you are sampling, so you dont have to make a slurry.
- so  you can just stick it into the substrate, or case- as long as its hydrated.
I guess its pricey by our standards but my current probe not trustworthy.



So I have one of these things for my succulents, but I've never used the pH meter for it. Would something like this be worth testing?

Which leads me to the next question... What am I paying attention to pH for in all this? Where is it useful?

These Pans seem like a huge pan in ass.

I'm starting to realize I may need to construct a custom rig for this grow. I have some neat fun ideas going through my head (I do like to build shit), so I'm glad I have some time while this shit germinates on agar.


Extras: Filter Print Post Top
Offlinezxcv101
Stranger
 User Gallery

Registered: 07/09/09
Posts: 64
Loc: SW
Last seen: 4 years, 22 days
Re: Official Copelandia/Panaeolus Thread [Re: ShaperDreaming]
    #25738062 - 01/11/19 10:57 AM (5 years, 19 days ago)

This doesn't look right. The mycelium colonized most of the way through my 1/4 inch  casing layer. I'm seeing lots of metabolites and I believe this is aerial mycelium. I'm assuming I've got FAE problems?







My casing was 50/50 peat verm. Cased 5 days ago.


Extras: Filter Print Post Top
InvisibleJohnny Dont
500 Don'ts of Knife Safety


Registered: 04/24/14
Posts: 1,830
Re: Official Copelandia/Panaeolus Thread [Re: zxcv101]
    #25738361 - 01/11/19 02:14 PM (5 years, 19 days ago)

So I think I finally have some clean cultures.
This one looks the best





I've been tryn to isolate away from some sorta contaminate that sorta looks matted once its grown out a bit. You can see it from about 6oclock to 9


Sense all of these are 1 transfer away from contaminanted dishes, I should do another round of transfers right? Or could I put the first dish to grains? And just do transfers on the other 3?


--------------------
I have left life and loves behind me, to be blown about as the sea desires, to have the freedom of the open air, and to be witness to the making of the world.


Extras: Filter Print Post Top
OfflineJakeoncid419
Follow me.....
Male User Gallery


Registered: 06/26/18
Posts: 4,390
Loc: Basel Switzerland
Last seen: 1 day, 17 hours
Trusted Cultivator
Re: Official Copelandia/Panaeolus Thread [Re: Asura]
    #25738401 - 01/11/19 02:37 PM (5 years, 19 days ago)

Quote:

Asura said:
Cranberry juice + 2g dried pans. Very simple formula to find the One Mind.

Come on, Jake...do it!



I’ll def give it a shot I ate about 4 gs in chocolate last night then while tripping wondered into my grow room and ate a few freshies our the tray lol I was out there but still came back 7 hours  later I love how clean pans are, they don’t give me a mind fuck even at high doses, super intense visuals very giggly love em


--------------------
Natural omt/detox
online  pant cult classes available last Saturday of every month go to buymeacoffee.com/jakeoncid to sign up (1 on 1 consultations also available
JOC PAN TEK
CORDYCEPS MILITARIS

EXOTICS
[


Extras: Filter Print Post Top
InvisibleJ. Jack Flash
stranger than ever.

Registered: 11/20/13
Posts: 1,500
Re: Official Copelandia/Panaeolus Thread [Re: ShaperDreaming]
    #25738413 - 01/11/19 02:46 PM (5 years, 19 days ago)

Quote:

ShaperDreaming said:
Quote:

mary fairchild said:I just ordered a nice pH meter, Apera Inst. it has a neat probe that you just stick into whatever you are sampling, so you dont have to make a slurry.
- so  you can just stick it into the substrate, or case- as long as its hydrated.
I guess its pricey by our standards but my current probe not trustworthy.



So I have one of these things for my succulents, but I've never used the pH meter for it. Would something like this be worth testing?

Which leads me to the next question... What am I paying attention to pH for in all this? Where is it useful?

These Pans seem like a huge pan in ass.

I'm starting to realize I may need to construct a custom rig for this grow. I have some neat fun ideas going through my head (I do like to build shit), so I'm glad I have some time while this shit germinates on agar.




this is my understanding. anyone who knows better, please correct me.

pan cyans, at least, require a casing to fruit. peat is often used as casing material. peat is acidic, which is bad for casing. so a pH buffer such as garden lime is added to raise the pH to just above neutral. (weakly alkaline) a pH tester is a means to decide how much of the buffer to add. i've opted to use test strips. i believe i read that mary fairchild found the strips not to be very reliable, tho.

garden lime is calcium carbonate. CaCO3. eggshells are also almost entirely CaCO3. as an observational experiment, i used powdered eggshells to buffer some jiffy mix (mostly peat) to use for a tiny cube project i have underway. test strips indicated success, and that project is pinning nicely right now. (i know, cubes don't need casing, but i want the experience)

relatedly: someone mentioned using baking soda, but that's trickier because of a quirk of carbonate chemistry.  CaCO3 dissolves poorly in water, but its solubility improves with increasing acidity. so an acidic solution dissolves enough of it to return it to a weakly alkaline state, at which point it stops dissolving, kind of automagically limiting pH increase, making it more forgiving of adding too much. baking soda, by comparison, is more soluble in water, thus much easier to raise pH too high.


--------------------
 
the j stands for jesus.
2020 new years grow along


Extras: Filter Print Post Top
Invisiblenube424
Male

Registered: 12/03/17
Posts: 6,063
Re: Official Copelandia/Panaeolus Thread [Re: nube424]
    #25738426 - 01/11/19 02:54 PM (5 years, 19 days ago)

Quote:

nube424 said:
Finally got enough colonization to snap a pic. Is this on par with pan bisp mycelium?


All the black specs are nijer seed. I didn't realize this brand of wbs had so much of it till It was too late. :facepalm3:



Bump? I'm really curious and concerned about how this mycelium looks. Any thoughts?


Extras: Filter Print Post Top
OfflineShaperDreaming
Weirdo
Female User Gallery


Registered: 10/30/18
Posts: 3,429
Loc: United States Flag
Last seen: 2 years, 3 days
Re: Official Copelandia/Panaeolus Thread [Re: J. Jack Flash]
    #25738441 - 01/11/19 02:58 PM (5 years, 19 days ago)

Thanks J.J! That makes sense. When you make/mix this casing do you do these measurements before pasteurization or after? Also, what's the pasteurization process for these items?


Extras: Filter Print Post Top
OfflineJakeoncid419
Follow me.....
Male User Gallery


Registered: 06/26/18
Posts: 4,390
Loc: Basel Switzerland
Last seen: 1 day, 17 hours
Trusted Cultivator
Re: Official Copelandia/Panaeolus Thread [Re: J. Jack Flash]
    #25738444 - 01/11/19 02:58 PM (5 years, 19 days ago)

Quote:

J. Jack Flash said:
Quote:

ShaperDreaming said:
Quote:

mary fairchild said:I just ordered a nice pH meter, Apera Inst. it has a neat probe that you just stick into whatever you are sampling, so you dont have to make a slurry.
- so  you can just stick it into the substrate, or case- as long as its hydrated.
I guess its pricey by our standards but my current probe not trustworthy.



So I have one of these things for my succulents, but I've never used the pH meter for it. Would something like this be worth testing?

Which leads me to the next question... What am I paying attention to pH for in all this? Where is it useful?

These Pans seem like a huge pan in ass.

I'm starting to realize I may need to construct a custom rig for this grow. I have some neat fun ideas going through my head (I do like to build shit), so I'm glad I have some time while this shit germinates on agar.




this is my understanding. anyone who knows better, please correct me.

pan cyans, at least, require a casing to fruit. peat is often used as casing material. peat is acidic, which is bad for casing. so a pH buffer such as garden lime is added to raise the pH to just above neutral. (weakly alkaline) a pH tester is a means to decide how much of the buffer to add. i've opted to use test strips. i believe i read that mary fairchild found the strips not to be very reliable, tho.

garden lime is calcium carbonate. CaCO3. eggshells are also almost entirely CaCO3. as an observational experiment, i used powdered eggshells to buffer some jiffy mix (mostly peat) to use for a tiny cube project i have underway. test strips indicated success, and that project is pinning nicely right now. (i know, cubes don't need casing, but i want the experience)

relatedly: someone mentioned using baking soda, but that's trickier because of a quirk of carbonate chemistry.  CaCO3 dissolves poorly in water, but its solubility improves with increasing acidity. so an acidic solution dissolves enough of it to return it to a weakly alkaline state, at which point it stops dissolving, kind of automagically limiting pH increase, making it more forgiving of adding too much. baking soda, by comparison, is more soluble in water, thus much easier to raise pH too high.



exactly and the casing's thickness is super important it must be kept thin! Ive been using peat and verm at 60/40 peat heavy balanced with hydrated lime (I use a analog ph meter probably not exactly accurate but as long as ur between 7-7.8 you will be fine. I also throw in a handful of cos and ive been noticing that a lot of the mushroom pop up under or near pieces of cos (idk if this is because the cos does not get colonized or what. (however ive ran tubs with no cos and been fine)
I used to cut potassium sulfate into my spawn as wll as a few other added chems along they way and eventually I will start trying them to see if I notice an improvement but as it is now I don't even add gypsum just spawn poo straw and verm cased with above mentioned


--------------------
Natural omt/detox
online  pant cult classes available last Saturday of every month go to buymeacoffee.com/jakeoncid to sign up (1 on 1 consultations also available
JOC PAN TEK
CORDYCEPS MILITARIS

EXOTICS
[


Extras: Filter Print Post Top
OfflineShaperDreaming
Weirdo
Female User Gallery


Registered: 10/30/18
Posts: 3,429
Loc: United States Flag
Last seen: 2 years, 3 days
Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
    #25738466 - 01/11/19 03:10 PM (5 years, 19 days ago)

If something is labeled "Food grade calcium hydroxide with no additives or preservatives" AKA "Food grade lime" ... can that be used in place of CaCO3 (why do I have a feeling this is a dumb question).


Edited by ShaperDreaming (01/11/19 03:11 PM)


Extras: Filter Print Post Top
InvisibleJ. Jack Flash
stranger than ever.

Registered: 11/20/13
Posts: 1,500
Re: Official Copelandia/Panaeolus Thread [Re: ShaperDreaming]
    #25738476 - 01/11/19 03:18 PM (5 years, 19 days ago)

no way. hydroxides are extremely alkaline. you'll make soap before mushrooms. you'll also suffer chemical burns. so no, not a dumb question.


--------------------
 
the j stands for jesus.
2020 new years grow along


Edited by J. Jack Flash (01/11/19 03:25 PM)


Extras: Filter Print Post Top
OfflineShaperDreaming
Weirdo
Female User Gallery


Registered: 10/30/18
Posts: 3,429
Loc: United States Flag
Last seen: 2 years, 3 days
Re: Official Copelandia/Panaeolus Thread [Re: J. Jack Flash]
    #25738488 - 01/11/19 03:25 PM (5 years, 19 days ago)

Quote:

J. Jack Flash said:
no way. hydroxides are extremely alkaline. you'll make soap before mushrooms. you'll also suffer chemical burns.



:horrified:


Extras: Filter Print Post Top
OfflineJakeoncid419
Follow me.....
Male User Gallery


Registered: 06/26/18
Posts: 4,390
Loc: Basel Switzerland
Last seen: 1 day, 17 hours
Trusted Cultivator
Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
    #25738512 - 01/11/19 03:37 PM (5 years, 19 days ago)

Ok so hydrated lime is really only used ever by small hobbie (usually active growers) under most other circumstances Hydrated lime should not (and is not) be used. Calcium carbonate is better safer and cheaper. depending on the grade of ur HL and how much u use you can easily fuck up ur shit. Some grades of hl are more caustic than others so like a box of chocolates.....
You can get Calcium carbonate powder In a few different forms. some garden stores usually call it garden lime, “ground oyster shells”, limestone, “any form of calcium-carbonate-based lime”. You do not want dolomite-base lime (magnesium-rich form). Ive also read that you can get it from wine making shops.  If you already have HL and ur nervous about using it spread it  about ½ inch thick on the bottom of  a glass pan (NOT metal)  cook at 350F for a hour. heat and CO2 in the air will covert most Of ur hL to calcium carbonate.


--------------------
Natural omt/detox
online  pant cult classes available last Saturday of every month go to buymeacoffee.com/jakeoncid to sign up (1 on 1 consultations also available
JOC PAN TEK
CORDYCEPS MILITARIS

EXOTICS
[


Edited by Jakeoncid419 (01/11/19 03:39 PM)


Extras: Filter Print Post Top
Jump to top Pages: < First | < Back | 356 | 357 | 358 | 359 | 360 | 361 | 362 | 363 | 364 | 365 | 366 | 367 | 368 | 369 | 370 | 371 | 372 | 373 | 374 | 375 | 376 | Next > | Last >

Shop: Bridgetown Botanicals CBD Concentrates   Kraken Kratom Red Vein Kratom   Original Sensible Seeds Autoflowering Cannabis Seeds   PhytoExtractum Kratom Powder for Sale   Left Coast Kratom Buy Kratom Capsules   North Spore Bulk Substrate   Mushroom-Hut Liquid Cultures


Similar ThreadsPosterViewsRepliesLast post
* The official Psilocybe natalensis thread
( 1 2 3 4 ... 515 516 )
Hillbillyjohn 328,300 10,316 01/29/24 06:30 PM
by TheAtlantean
* Official Misc EXOTIC Thread
( 1 2 3 4 ... 132 133 )
Jakeoncid419 110,126 2,651 01/29/24 04:42 PM
by EniQma
* Rye-Z-Morph Bag for Panaeolus (Copelandia) Cyanescens? rob_531 4,313 12 01/10/05 12:33 PM
by agar
* Copelandia "Pan" Cyanescens sancho 4,962 6 09/07/07 10:00 AM
by suave
* Re: look at these copelandia cyanescens they are beautiful Anonymous 5,161 2 12/25/99 07:16 AM
by sean123
* which tek for copelandia cyanescens (spore print)? FfF 7,484 10 08/26/04 05:44 AM
by hawksapprentice
* Hawaiian Copelandia Grow Kit - how much more difficult are they? GabrielKnight 8,125 5 01/03/05 04:59 PM
by ticktock
* Preparing jars for Copelandia? PawPaw 1,195 14 04/02/05 01:11 PM
by PawPaw

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
924,141 topic views. 40 members, 235 guests and 36 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.038 seconds spending 0.016 seconds on 15 queries.