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OfflineAdas
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Re: Official Copelandia/Panaeolus Thread [Re: mushboy]
    #25733503 - 01/09/19 09:36 AM (5 years, 21 days ago)

Maybe you are just pouring thick.


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OfflineShaperDreaming
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Re: Official Copelandia/Panaeolus Thread [Re: mushboy]
    #25733511 - 01/09/19 09:41 AM (5 years, 21 days ago)

Quote:

ShaperDreaming said:
damn dude those plates are super clear for grain water. my grain water dishes come out looking like dark pee:blush:



Really? They were coming out clearer earlier in the pour when it was still warmer. What grains do you use? This is from a 28 min boil on oats. I collected the "oat juice" when making the grains and let it sit overnight, then tried to pour off the top cleaner part when transferring to media bottles.

Plate growth is great at this rate, but a little too arial for my liking. I also had an issue where the myc didn't have a good grip on the surface and the slice I cut had the little guy fall off in the transfer. I'm going to drop the agar so the myc can get a better foothold.


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OfflineJakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: ShaperDreaming]
    #25733563 - 01/09/19 10:06 AM (5 years, 21 days ago)

I really like peptone for agar you can use less of it than other nutes and ur dishes are almost clear I often do mix in some malt also tho just to save peptone as its the most expensive of all my nutes


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InvisiblemushboyMDiscord
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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
    #25733584 - 01/09/19 10:16 AM (5 years, 21 days ago)

i just do light malt. mix cold pc 15min. clear as glass.


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OfflineShaperDreaming
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Re: Official Copelandia/Panaeolus Thread [Re: mushboy]
    #25733589 - 01/09/19 10:18 AM (5 years, 21 days ago)

Quote:

mushboy said:
i just do light malt. mix cold pc 15min. clear as glass.




Looks like something I drank as a kid :slimer:

Cool! What amount of lme do you use?


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Invisiblemary fairchild
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Re: Official Copelandia/Panaeolus Thread [Re: dmar]
    #25733594 - 01/09/19 10:19 AM (5 years, 21 days ago)

Quote:

dmar said:
Now I just need a print... Anybody wanna hook me up with one? :grin:




Genetics,
A print, is there something specific you'd like?


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Invisiblenube424
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Re: Official Copelandia/Panaeolus Thread [Re: mary fairchild]
    #25733632 - 01/09/19 10:35 AM (5 years, 21 days ago)

What is the correct spawn ratio for say, pan bisporus with grains? Do they like high or low spawn ratios?

Pans in general. I only said bisporus cuz that's what I have :rockon:


Edited by nube424 (01/09/19 10:36 AM)


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Invisiblemary fairchild
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Re: Official Copelandia/Panaeolus Thread [Re: nube424]
    #25733763 - 01/09/19 11:37 AM (5 years, 21 days ago)

For bis I did a ~1:2.5 v/v recently. As far as cyans- I went out to 1:4 and it supported fruiting just as well as 1:2, that implies to me that we may be over saturating the substrate with higher spawn ratios.


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Invisiblenube424
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Re: Official Copelandia/Panaeolus Thread [Re: mary fairchild]
    #25733770 - 01/09/19 11:41 AM (5 years, 21 days ago)

Hmm. Thanks Mary. I'll try 1:2.5 for this first go.

Also, how did your bisporus look on grain? Mine is so thin and fuzzy it's starting to freak me out. Lol. I'm used to cubes.


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Invisiblemary fairchild
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Re: Official Copelandia/Panaeolus Thread [Re: nube424]
    #25733776 - 01/09/19 11:48 AM (5 years, 21 days ago)



I wouldn't necessarily be too concerned if yours looks a bit lighter, there's always some variability in what it looks like from different genetics- what you get in the jar reflects the form you get on agar.


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Invisiblenube424
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Re: Official Copelandia/Panaeolus Thread [Re: mary fairchild]
    #25733780 - 01/09/19 11:51 AM (5 years, 21 days ago)

Hmmm thanks. I'll post pics when it grows out a little more. Just shook it 2 days ago. Shook a little early but fuck it, it recovered fine.


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OfflineJakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: nube424]
    #25733891 - 01/09/19 12:46 PM (5 years, 21 days ago)

Yeah I try to cut my pan spawn in at no more than 1:4 seems less susceptible to overlay but maybe that’s just coincidence


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Edited by Jakeoncid419 (01/09/19 12:46 PM)


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Invisiblenube424
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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
    #25733938 - 01/09/19 01:21 PM (5 years, 21 days ago)

Hmm. I got 4 qts. That could go a long way at 1:4


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Invisiblemary fairchild
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Re: Official Copelandia/Panaeolus Thread [Re: nube424]
    #25733981 - 01/09/19 01:41 PM (5 years, 21 days ago)

I've never gone farther out than 1:4 for Spawn to Bulk procedure- but I have some reason to believe you could go out quit a bit further- ie. 1:8.

The consideration from cube growing that also applies to Pans is that these much lower ratios may be more prone to contamination.

Regardless- a 1:4 is still plenty of spawn, my vote is for a 1:4.


Edited by mary fairchild (01/09/19 02:03 PM)


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OfflineJakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: mary fairchild]
    #25734018 - 01/09/19 02:07 PM (5 years, 21 days ago)

Quote:

mary fairchild said:
I've never gone farther out than 1:4 for Spawn to Bulk procedure- but I have some reason to believe you could go out quit a bit further- ie. 1:8.

The consideration from cube growing that also applies to Pans is that these much lower ratios may be more prone to contamination.

Regardless- a 1:4 is still plenty of spawn, my vote is for a 1:4.



I cut my cubes in stronger, but the pan’s  moves so fast I feel 1 to 4 is plenty


--------------------
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JOC PAN TEK
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EXOTICS
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Re: Official Copelandia/Panaeolus Thread [Re: mary fairchild]
    #25734022 - 01/09/19 02:09 PM (5 years, 21 days ago)

I'm going to try a bulk spawn in bags instead of spawning grains to bulk for my next run. I notice all of the pros seem to use WBS, mixed in dry. I have a lot of rye grain. Any thoughts on subbing in rye grain for WBS in these bulk recipes? I'm looking at yours at the moment Mary:

Substrate-> Spawn bags:
Straw 75g.
Dehydrated Horse Manure 675g.
Wild Bird Seed 200g (3.75X less than Horse/Straw w/w)
Vermiculite 156g
Gypsum 20g
Azomite 10g(trace minerals)
Total weight of dry components 1146g
H2O ~1940g
Split substrate evenly to two large spawn bags
PC’d 4hr@15psi.

How do you think this would go with a similar amount of rye grain in there instead of WBS? Would you use more or less or the same? Would you hydrate it first or no?

In other news one of my trop trays got hit by trich two days after casing. I'm pretty sure it came from above and not below as it happened very soon after casing and looks like it's growing directly on top, but can't be sure. Perhaps I did not pasteurize the casing effectively as it was on the short end of things and on the lower end of temps, however the other two trays are still fine and it is four days later and they look like they might start pinning soon (currently day 6 since casing). Fingers are crossed.


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Invisiblenube424
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Re: Official Copelandia/Panaeolus Thread [Re: space_shrooms]
    #25734042 - 01/09/19 02:25 PM (5 years, 21 days ago)

I'll do 1:4 and 1:2. Just for fun.

Also, I have sphangum peat moss AND cactus soil already. And I know both have been used as a casing. I was thinking of trying 50/50 with those ingredients. All I'd need to get is lime.

Also, This is definitely a dumb question but why couldn't baking soda be used instead of lime?


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Invisiblemary fairchild
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Re: Official Copelandia/Panaeolus Thread [Re: nube424]
    #25734122 - 01/09/19 03:09 PM (5 years, 21 days ago)

Baking soda-eek, SODIUM bicarbonate- I doubt mycelia see much sodium in the wild. It even tastes real salty.

Why not straight cactus soil alone?

If you have a health food store nearby you might be able to get some calcium carbonate- thats where I get mine.


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OfflineJakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: mary fairchild]
    #25734125 - 01/09/19 03:12 PM (5 years, 21 days ago)

hey pan-handlers (hehe) so I put the word out that I wanted subG prints and the myco world all responded 3 weeks later at once lol so I have at least 1 extra sub G print. I lost my clean pan cam culture who wants to trade dis for dat, (cam print of course)


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Invisiblemary fairchild
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Re: Official Copelandia/Panaeolus Thread [Re: space_shrooms]
    #25734150 - 01/09/19 03:28 PM (5 years, 21 days ago)

Quote:

space_shrooms said:
I'm going to try a bulk spawn in bags instead of spawning grains to bulk for my next run. I notice all of the pros seem to use WBS, mixed in dry. I have a lot of rye grain. Any thoughts on subbing in rye grain for WBS in these bulk recipes? I'm looking at yours at the moment Mary:

Substrate-> Spawn bags:
Straw 75g.
Dehydrated Horse Manure 675g.
Wild Bird Seed 200g (3.75X less than Horse/Straw w/w)
Vermiculite 156g
Gypsum 20g
Azomite 10g(trace minerals)
Total weight of dry components 1146g
H2O ~1940g
Split substrate evenly to two large spawn bags
PC’d 4hr@15psi.

How do you think this would go with a similar amount of rye grain in there instead of WBS? Would you use more or less or the same? Would you hydrate it first or no?

In other news one of my trop trays got hit by trich two days after casing. I'm pretty sure it came from above and not below as it happened very soon after casing and looks like it's growing directly on top, but can't be sure. Perhaps I did not pasteurize the casing effectively as it was on the short end of things and on the lower end of temps, however the other two trays are still fine and it is four days later and they look like they might start pinning soon (currently day 6 since casing). Fingers are crossed.




Thing about grain- they talk about "bacterial endospores (this comes from Roger Rabbit). So thats why we go thru the longer hydrating before sterilization process. The protocol above is adapted from Blue Helix, its so easy to just throw in some raw WBS & let it hydrate in the pressure cooker.
And just to go back to talking about the grain to sub ratios- by volume thats a very small amount of grain to sub. ratio.

I actually have done it- just like you say- hydrate the rye grain- just like they say then add it to that recipe but- since its weight is ~doubled from the hydrating process- I added twice the weight as the dry WBS.
It worked the same- the sub colonized about the same. IMO its not worth the extra work.

The contamination is a bummer:sad:. When I was pasteurizing I was pretty strict about it- did it in jars in a water bath, 2hr @ 140->165F.


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