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Psilosopherr
A psilly goose



Registered: 02/15/12
Posts: 12,278
Last seen: 1 month, 10 days
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Re: Official Copelandia/Panaeolus Thread [Re: Brain Bulb]
#25730387 - 01/07/19 09:48 PM (5 years, 22 days ago) |
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nice troubleshooting, never heard of such an approach
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Asura
Cyantist


Registered: 08/01/11
Posts: 5,047
Loc: Texas
Last seen: 11 days, 21 hours
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Re: Official Copelandia/Panaeolus Thread [Re: Brain Bulb]
#25730392 - 01/07/19 09:51 PM (5 years, 22 days ago) |
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Quote:
Brain Bulb said: Woohoo!!
Haven’t had much good news to report on the Pan front lately, but came home to some progress today.
My Aussie SB had stalled out and wouldn’t colonize the casing... So, I decided to mix a small amount of CaCO3 (which I forgot to add initially) with distilled water, put it into a spray bottle and mist the casing. It’s working!... so far. Sub still looks good, healthy myc, no contaminants so I’m hoping this is going to produce some fruit.
These are from Friday night around 9:30 pm: (Didn’t look much different last night)
 
Misted with CaCO3 water last night and came home to this:

Know it’s not much but it’s a step in the right direction. Hope to have some more updates over the next few days.:-)
Maybe mary can chime in if I'm wrong, but I don't think you want the casing to be colonized.
This is a pic one of my trays with the first flush coming in:

If you look at the casing, you'll see it's not colonized.
This is the same tray with the 3rd flush coming in. Same thing.
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Jakeoncid419
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Registered: 06/26/18
Posts: 4,390
Loc: Basel Switzerland
Last seen: 1 day, 17 hours
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Re: Official Copelandia/Panaeolus Thread [Re: Asura]
#25730459 - 01/07/19 10:31 PM (5 years, 22 days ago) |
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Quote:
Asura said:
Quote:
Brain Bulb said: Woohoo!!
Haven’t had much good news to report on the Pan front lately, but came home to some progress today.
My Aussie SB had stalled out and wouldn’t colonize the casing... So, I decided to mix a small amount of CaCO3 (which I forgot to add initially) with distilled water, put it into a spray bottle and mist the casing. It’s working!... so far. Sub still looks good, healthy myc, no contaminants so I’m hoping this is going to produce some fruit.
These are from Friday night around 9:30 pm: (Didn’t look much different last night)
 
Misted with CaCO3 water last night and came home to this:

Know it’s not much but it’s a step in the right direction. Hope to have some more updates over the next few days.:-)
Maybe mary can chime in if I'm wrong, but I don't think you want the casing to be colonized.
This is a pic one of my trays with the first flush coming in:

If you look at the casing, you'll see it's not colonized.
This is the same tray with the 3rd flush coming in. Same thing.

yeah u might see some poking thru but if it solid colonizes ur casing ur just gonna get overly
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Jakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
#25730472 - 01/07/19 10:36 PM (5 years, 22 days ago) |
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so then martha pulling air in is working well however the one with the fan blowing in can only effectively produce cubes.... its odd should have plenty of fae maybe it was the trays anyway heres the good one tamp pan cube chessnut
-------------------- Natural omt/detox online pant cult classes available last Saturday of every month go to buymeacoffee.com/jakeoncid to sign up (1 on 1 consultations also available JOC PAN TEK CORDYCEPS MILITARIS EXOTICS [
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Psilosopherr
A psilly goose



Registered: 02/15/12
Posts: 12,278
Last seen: 1 month, 10 days
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Re: Official Copelandia/Panaeolus Thread [Re: Asura]
#25730492 - 01/07/19 10:46 PM (5 years, 22 days ago) |
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with that in mind maybe improper ph in a casing would be desirable so as to prevent overlay
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Jakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: Psilosopherr]
#25730504 - 01/07/19 10:51 PM (5 years, 22 days ago) |
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Quote:
Psilosopherr said: with that in mind maybe improper ph in a casing would be desirable so as to prevent overlay
no its more y u are told to put pans into fc immediately after casing the condition will switch the myc from colonizing to fruiting, adding sum cos helps as it cant be colonized
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ShaperDreaming
Weirdo



Registered: 10/30/18
Posts: 3,429
Loc: United States
Last seen: 2 years, 3 days
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Quote:
mary fairchild said: I never grew Trops- for awhile I was playing around with plating from a difficult spore syringe, then lost interest. I'm pretty sure 99 did a nice grow of Trops ~ a year ago. I think VL and maybe Doc grew them also. They are getting higher on my list now.
Looks like I'm off to figure this out on my own. I guess I picked the wrong random syringe... for some reason I grabbed it and thought "huh... I think this is one of the easier exotics! I'll try it out."
Shows what I know. I guess I report on my findings/failings. That plate I started isn't looking .... so hot. I'll wait and see tho.
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bug_handler
Stranger
Registered: 12/04/18
Posts: 3
Last seen: 4 years, 9 months
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Greetings. I've just started using agar and make my first mistakes (inoculating with syringe instead of just transferring spores, thus creating excess water in the plate). So, three plates which have been inoculated with Panaeolus tropicalis syringe now have some signs of life. I need your help identifying it. It seems like young Aspergillus flavus colonies to me. I wish I'm wrong.

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nube424


Registered: 12/03/17
Posts: 6,063
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Re: Official Copelandia/Panaeolus Thread [Re: bug_handler]
#25731177 - 01/08/19 09:24 AM (5 years, 22 days ago) |
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Looks like pan mycelium to me. It's alot thinner than cube mycelium. Definitely looks like mold at first.
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ShaperDreaming
Weirdo



Registered: 10/30/18
Posts: 3,429
Loc: United States
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Re: Official Copelandia/Panaeolus Thread [Re: nube424]
#25731215 - 01/08/19 09:47 AM (5 years, 22 days ago) |
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Quote:
nube424 said: Definitely looks like mold at first.
This is good to read! I was looking at my inoculation loop work and saw that everywhere it touched looked like it may be spawning something not good looking... I thought maybe the whole syringe may be done.
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nube424


Registered: 12/03/17
Posts: 6,063
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Yea it's strange looking. I have bisporus on agar and on grains right now and it looks just like cobweb. It's very hairy and thin and sorta grayish. Actually more "clear" than grey.
 Sorry about the pic quality. But u get the gist
Edited by nube424 (01/08/19 09:51 AM)
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ShaperDreaming
Weirdo



Registered: 10/30/18
Posts: 3,429
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Re: Official Copelandia/Panaeolus Thread [Re: nube424]
#25731282 - 01/08/19 10:19 AM (5 years, 22 days ago) |
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Quote:
nube424 said: Yea it's strange looking.
Oh, I'm not that far, mine is still ... uhm "germinating"/just starting. It kinda looks like off-cream colored bacteria? I mean, really it looks almost like nothing except some condensation where I noc'ced it up.
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nube424


Registered: 12/03/17
Posts: 6,063
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Hmm. Got a pic? It could just be a mark from where u swiped it. Sometimes I see streaked where I swipe.
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ShaperDreaming
Weirdo



Registered: 10/30/18
Posts: 3,429
Loc: United States
Last seen: 2 years, 3 days
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Re: Official Copelandia/Panaeolus Thread [Re: nube424]
#25731332 - 01/08/19 10:45 AM (5 years, 22 days ago) |
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I'll grab a picture when I'm home later tonight. I thought I had, but ... nope!
Also, I started a journal about this grow. I just wanted to consolidate all the research I've done so far mainly so I can reference it quickly. I'll also take/give prints if I ever get there!
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mary fairchild
Pantheist


Registered: 12/06/14
Posts: 777
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Re: Official Copelandia/Panaeolus Thread [Re: Asura]
#25731514 - 01/08/19 12:11 PM (5 years, 22 days ago) |
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Quote:
Asura said:
Quote:
Brain Bulb said: Woohoo!!
Haven’t had much good news to report on the Pan front lately, but came home to some progress today.
My Aussie SB had stalled out and wouldn’t colonize the casing... So, I decided to mix a small amount of CaCO3 (which I forgot to add initially) with distilled water, put it into a spray bottle and mist the casing. It’s working!... so far. Sub still looks good, healthy myc, no contaminants so I’m hoping this is going to produce some fruit.
These are from Friday night around 9:30 pm: (Didn’t look much different last night)
 
Misted with CaCO3 water last night and came home to this:

Know it’s not much but it’s a step in the right direction. Hope to have some more updates over the next few days.:-)
Maybe mary can chime in if I'm wrong, but I don't think you want the casing to be colonized.
This is a pic one of my trays with the first flush coming in:

If you look at the casing, you'll see it's not colonized.
This is the same tray with the 3rd flush coming in. Same thing.

I've had good results, or bad results with or without any colonization of the case. When you dont get colonization of the case it just means that the mycelia's propagation stopped either because it switched to fruiting mode or it just stalled, in this case it obviously switched to fruiting mode. I've had grows that started to overlay (produced some hardened mats) & then overlay halted and they fruited.
BTW, Asuras grow rocks! The protocol clearly works.
Edited by mary fairchild (01/08/19 12:34 PM)
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nube424


Registered: 12/03/17
Posts: 6,063
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I've seen a few people use cactus soil as a casing? I have cactus soil and peat. Should I mix them? Or try using both but separate?
Also I was wondering if anything other than lime can be used to correct the ph?
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Tweeq
Tweeq of Nature


Registered: 06/07/18
Posts: 2,043
Loc: Netherlands
Last seen: 5 hours, 11 minutes
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Re: Official Copelandia/Panaeolus Thread [Re: nube424]
#25731622 - 01/08/19 01:13 PM (5 years, 22 days ago) |
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Guys, a (noob) question. We (me and my girlfriend) are busy rearranging our living space to be as prepared as we can before our new growing adventure takes off. That's all great but as the actual main reason for our getting into this is an experience with pan cyan hawaii, that is also the species we would eventually like to cultivate. Sure we like the cubes too but these are just somethimg else! So as pan growers, what would you advise us to start out with? Start with cubes and work towards more difficult species later, or should we boldly go for pans straight away? Maybe try both simultanuously? For who's interested some pics of our nearing completion setup are in my journal. Hope to get some advice here as we are just about ready to get started.
In short: what would be the better option if the end goal is growing pan cyan hawaii
Edited by Tweeq (01/08/19 01:23 PM)
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Jakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: Tweeq]
#25731649 - 01/08/19 01:28 PM (5 years, 22 days ago) |
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Quote:
Tweeq said: Guys, a (noob) question. We (me and my girlfriend) are busy rearranging our living space to be as prepared as we can before our new growing adventure takes off. That's all great but as the actual main reason for our getting into this is an experience with pan cyan hawaii, that is also the species we would eventually like to cultivate. Sure we like the cubes too but these are just somethimg else! So as pan growers, what would you advise us to start out with? Start with cubes and work towards more difficult species later, or should we boldly go for pans straight away? Maybe try both simultanuously? For who's interested some pics of our nearing completion setup are in my journal. Hope to get some advice here as we are just about ready to get started.
In short: what would be the better option if the end goal is growing pan cyan hawaii
Start with cubes, practice good sterile technique learn how mycelium works then I would recommend trying tamps next they are a lil more tricky than cubes but easier than pans ime. You could start right out the gate with pans but it will be a much steeper learning curve before you get fruits
-------------------- Natural omt/detox online pant cult classes available last Saturday of every month go to buymeacoffee.com/jakeoncid to sign up (1 on 1 consultations also available JOC PAN TEK CORDYCEPS MILITARIS EXOTICS [
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Tweeq
Tweeq of Nature


Registered: 06/07/18
Posts: 2,043
Loc: Netherlands
Last seen: 5 hours, 11 minutes
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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
#25731683 - 01/08/19 01:44 PM (5 years, 22 days ago) |
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Thanks. That was my guess too but you're the ones who really know. Thinking of starting with hpoo and straw as bulk substrate though as that is what i should learn to work with anyway, if im not wrong
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Jakeoncid419
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Registered: 06/26/18
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Re: Official Copelandia/Panaeolus Thread [Re: Tweeq]
#25731695 - 01/08/19 01:49 PM (5 years, 22 days ago) |
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Quote:
Tweeq said: Thanks. That was my guess too but you're the ones who really know. Thinking of starting with hpoo and straw as bulk substrate though as that is what i should learn to work with anyway, if im not wrong
Nothing wrong with that at all be sure to shred ur straw, cut in poo around 30% for cubes, you could just run str8 strAw but for tubs I like the strW poo verm mix it hold moisture better. I like coco coir best for cubes but it’s much more expensive
-------------------- Natural omt/detox online pant cult classes available last Saturday of every month go to buymeacoffee.com/jakeoncid to sign up (1 on 1 consultations also available JOC PAN TEK CORDYCEPS MILITARIS EXOTICS [
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