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Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
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shroomms
Mr. Shrooms
Registered: 07/27/14
Posts: 202
Last seen: 1 month, 6 days
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Re: Official Copelandia/Panaeolus Thread *DELETED* [Re: Asura]
#25695950 - 12/22/18 01:12 PM (5 years, 1 month ago) |
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Post deleted by shroomms
Reason for deletion: I wanted to! Thank-You!
Edited by shroomms (12/22/18 02:24 PM)
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Psilosopherr
A psilly goose



Registered: 02/15/12
Posts: 12,278
Last seen: 1 month, 10 days
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Quote:
mary fairchild said: Heat mats are often a dirty word on the site but they have helped me save money on my electric bill, you just have to be careful how you use them.
better watch out or they'll yank your TC tag
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Brain Bulb
Insane in the Membrane



Registered: 11/09/17
Posts: 1,358
Last seen: 6 months, 24 days
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Re: Official Copelandia/Panaeolus Thread [Re: Psilosopherr]
#25696853 - 12/22/18 08:09 PM (5 years, 1 month ago) |
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Well I came home to some positive news on my Aussie grow!:-). Think I’m finally getting the fruiting conditions dialed in. I’ve been tweaking things the last couple days and today is the first time in over a week I’ve seen new growth.
Like @MF and @Asura mentioned I focused on the room itself and it’s working. My thermostat was reading 75F with 99% humidity, but when I scanned the surface of the casing using my IR thermometer it came up @ 80.4F.

Also, the GS I inoculated with LI I made from this plate has colonized and is ready to spawn!:-)


@MaryFairchild and @Asura, you rock!
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mary fairchild
Pantheist


Registered: 12/06/14
Posts: 777
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Re: Official Copelandia/Panaeolus Thread [Re: shroomms]
#25696871 - 12/22/18 08:21 PM (5 years, 1 month ago) |
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Quote:
shroomms said: Asura, you mentioned that after you case it, the conditions are WET on the substrate before pinning. Do you mean the substrate surface has drops of visible water on it? what is your procedure (misting/saturating the casing?) on how you get it WET?
I'm interested in how you prepare the casing layer (for fruiting) regarding water content and whether misting was carried out?
Thanks-a-ton if you or anyone else could help me out in this area!
Hi Shrooms, Yeah, that's what i've been doing and I think Asura and I are on close to the same page. So you case it- I always give the case a good misting- don't want standing water but I like the case to almost glisten. So then its in to the fruiting chamber. The conditions that I'd like to get is high humidity AND high evaporation rate. So with enough fast air exchange you can get good evaporation even tho the air just above the case is close to 100% humidity.
This is the way I do it and I think its not too far from how Asura does it. Its kinda hard to get really high humiditys and high temperatures. Asura has really good results at lower temperatures and lower humidity so dont worry too much about that. We have greenhouse setups and we can think along those lines. If you're fruiting chamber is a mono tub or SGFC, then you won't get that kind of air exchange but it can work really well that way too.
Every day I take a look at the case- usually the edges start to look dry so I mist them. Keep it that way until pins appear- once you have a lot of pins switch to syringe watering the surface of the case. If the humidity was real high - lower it to ~95%.
Is all this stuff "for sure written in stone"- not at all.
Best wishes for the grow and Happy Holidays! mf
Edited by mary fairchild (12/22/18 08:29 PM)
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mary fairchild
Pantheist


Registered: 12/06/14
Posts: 777
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Re: Official Copelandia/Panaeolus Thread [Re: Brain Bulb]
#25696881 - 12/22/18 08:28 PM (5 years, 1 month ago) |
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Quote:
Brain Bulb said: Well I came home to some positive news on my Aussie grow!:-). Think I’m finally getting the fruiting conditions dialed in. I’ve been tweaking things the last couple days and today is the first time in over a week I’ve seen new growth.
Like @MF and @Asura mentioned I focused on the room itself and it’s working. My thermostat was reading 75F with 99% humidity, but when I scanned the surface of the casing using my IR thermometer it came up @ 80.4F.

Also, the GS I inoculated with LI I made from this plate has colonized and is ready to spawn!:-)


@MaryFairchild and @Asura, you rock!
Awesome, I was hoping to hear from you. I'm stoked!
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Brain Bulb
Insane in the Membrane



Registered: 11/09/17
Posts: 1,358
Last seen: 6 months, 24 days
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Thanks Mary. Was thinking I’d have to start over but am optimistic now.
Question for you. When I made my casing I didn’t have any CaCO3 but do now. Can I mix it up in my misting bottle to add? Also the instructions for the pH test strips I got are in Korean I believe. How do I properly test my pH?
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shroomms
Mr. Shrooms
Registered: 07/27/14
Posts: 202
Last seen: 1 month, 6 days
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Re: Official Copelandia/Panaeolus Thread *DELETED* [Re: Brain Bulb]
#25697670 - 12/23/18 08:54 AM (5 years, 1 month ago) |
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Post deleted by shroomms
Reason for deletion: I wanted to! Thank-You!
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Brain Bulb
Insane in the Membrane



Registered: 11/09/17
Posts: 1,358
Last seen: 6 months, 24 days
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Re: Official Copelandia/Panaeolus Thread [Re: shroomms]
#25697698 - 12/23/18 09:04 AM (5 years, 1 month ago) |
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Quote:
shroomms said: Related to Brain Bulb's question, approximately how much Calcium Carbonate are you adding to say 1 qt of casing material? Thank-you very much for any help.
Here’s the casing mix Mary used on an Aussie grow. Check out her documented grow logs. Lots of good stuff.
Case: 50% peat moss:50% vermiculite, CaCO3 to pH~7.5.
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shroomms
Mr. Shrooms
Registered: 07/27/14
Posts: 202
Last seen: 1 month, 6 days
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Re: Official Copelandia/Panaeolus Thread *DELETED* [Re: Brain Bulb]
#25697779 - 12/23/18 09:37 AM (5 years, 1 month ago) |
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Post deleted by shroomms
Reason for deletion: I wanted to! Thank-You!
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nube424


Registered: 12/03/17
Posts: 6,063
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Re: Official Copelandia/Panaeolus Thread [Re: shroomms]
#25697784 - 12/23/18 09:39 AM (5 years, 1 month ago) |
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It depends on the volume of casing. I'd start with a tablespoon and check it.
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shroomms
Mr. Shrooms
Registered: 07/27/14
Posts: 202
Last seen: 1 month, 6 days
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Re: Official Copelandia/Panaeolus Thread *DELETED* [Re: nube424]
#25697835 - 12/23/18 09:57 AM (5 years, 1 month ago) |
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Post deleted by shroomms
Reason for deletion: I wanted to! Thank-You!
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nube424


Registered: 12/03/17
Posts: 6,063
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Re: Official Copelandia/Panaeolus Thread [Re: shroomms]
#25697847 - 12/23/18 10:01 AM (5 years, 1 month ago) |
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Try a pinch. 1/4 to 1/2 teaspoon.
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Geinstein
Shroomery addict



Registered: 01/25/18
Posts: 1,784
Last seen: 2 years, 1 month
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Re: Official Copelandia/Panaeolus Thread [Re: nube424]
#25697909 - 12/23/18 10:30 AM (5 years, 1 month ago) |
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Can someone point me to a tek?
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Nothing breads nothing
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Brain Bulb
Insane in the Membrane



Registered: 11/09/17
Posts: 1,358
Last seen: 6 months, 24 days
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Re: Official Copelandia/Panaeolus Thread [Re: Geinstein]
#25697966 - 12/23/18 10:48 AM (5 years, 1 month ago) |
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Quote:
Geinstein said: Can someone point me to a tek?
What species?
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mary fairchild
Pantheist


Registered: 12/06/14
Posts: 777
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Re: Official Copelandia/Panaeolus Thread [Re: Brain Bulb]
#25698130 - 12/23/18 12:06 PM (5 years, 1 month ago) |
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Quote:
Brain Bulb said: Thanks Mary. Was thinking I’d have to start over but am optimistic now.
Question for you. When I made my casing I didn’t have any CaCO3 but do now. Can I mix it up in my misting bottle to add? Also the instructions for the pH test strips I got are in Korean I believe. How do I properly test my pH?
For the case- I guess you already hydrated it so if you spray it with a CaCO3/H2O mix it may be swimming. Better to just add the appropriate amount of CaCO3 directly to the hydrated case and stir well to mix it in. Important- don't just dump the CaCO3 in one spot before mixing, but try to spread it around because it tends to clump which makes it harder to mix it uniformly, MIX IT WELL.
The container that the test strips are in should have the different colors that correspond to pH values, Are the pH values in Korean?- I guess you can Google it. I pH'd a 50/50+ case and the pH comes out to ~ the 4.5->5 range. Pretty acidic, IDK what the mycelia thinks of that when it comes time to shift to fruiting. This is a critical stage- i'd be careful, Try to get it to at least ~7 but less than 8.
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mary fairchild
Pantheist


Registered: 12/06/14
Posts: 777
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Re: Official Copelandia/Panaeolus Thread [Re: shroomms]
#25698151 - 12/23/18 12:20 PM (5 years, 1 month ago) |
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Quote:
shroomms said: Related to Brain Bulb's question, approximately how much Calcium Carbonate are you adding to say 1 qt of casing material? Thank-you very much for any help.
You should be able to scale up from this recipe of 50/50+: 1.5cups peat 1.5cups verm 275ml H2O 1tsp CaCO3
But- to consider, i've been long using pH test strips, and that recipe gets me to a pH of between 7 and 7.5 by reading the color on the test strip. I recently got a cheap-o pH meter that I trust because I trust the calibration. So by pH meter that recipe only gives a pH of ~6.5. So the results I've been getting are actually from a pH of ~6.5. So it works but I plan to go by the meter for now and will try to get the pH into the "more optimal range" of 7 to 7.5.
This must be a confusion factor- but i'm always learning too.
So I just did the conversion- FOR 1QT. USE AT LEAST 2TSP, even 4 tsp shouldn't hurt since CaCO3 is pretty weak (safe).
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rickyswamps
Bad Apple



Registered: 11/08/18
Posts: 1,192
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I'd love to get some experienced eyes on these agar plates. They are first transfers from spore germination plates. I've noticed that I'm getting 2-3 distance types of mycelium, do they all ok to use to inoculate grain?

Here are some close ups
 Numbers 2,3,6, and 9 are similar. Dense and fluffy

 4,5,7,8 are a lot different, whispy, but moving faster across agar
Any thoughts or experience with plates that look like these?
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J. Jack Flash
stranger than ever.

Registered: 11/20/13
Posts: 1,500
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Re: Official Copelandia/Panaeolus Thread [Re: rickyswamps] 1
#25698820 - 12/23/18 06:37 PM (5 years, 1 month ago) |
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i'm a long way from experienced, but fotos 2 and 3 don't look ok to me. totally disorganized, i think they look like mold. plate 1 looks good.
deffo wait for a second opinion.
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mary fairchild
Pantheist


Registered: 12/06/14
Posts: 777
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Re: Official Copelandia/Panaeolus Thread [Re: rickyswamps]
#25699081 - 12/23/18 09:38 PM (5 years, 1 month ago) |
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Quote:
rickyswamps said: I'd love to get some experienced eyes on these agar plates. They are first transfers from spore germination plates. I've noticed that I'm getting 2-3 distance types of mycelium, do they all ok to use to inoculate grain?

Here are some close ups
 Numbers 2,3,6, and 9 are similar. Dense and fluffy

 4,5,7,8 are a lot different, whispy, but moving faster across agar
Any thoughts or experience with plates that look like these?
From the pics- pic 2 looks perfect, (esp. for 1st passage), pics 3 & 4 don't look right at all.
Mycelia normally should appear as strands radiating out from the center- it could just be that the pic is out of focus but that growth doesn't appear that way. I've seen that growth pattern before and I steer away from it. As far as more cottony vs more wispy, I wouldn't assume that the more cottony form is necessarily more likely to fruit well- if possible i'd take both forms downstream.
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Doc9151
Mycologist



Registered: 02/23/17
Posts: 13,753
Loc: Gulf Coast USA
Last seen: 1 year, 6 months
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Heat the room NOT the substrate.
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  Psilocybe cubensis data collection thread. please help with this project if you hunt wild cubensis. https://www.shroomery.org/forums/showflat.php?Cat=0&Number=26513593&page=0&vc=1#26513593
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