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Asura
Cyantist


Registered: 08/01/11
Posts: 5,047
Loc: Texas
Last seen: 11 days, 17 hours
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Quote:
mary fairchild said: Just like to comment on what i've experienced with grow bags. I have been mostly using the ones that have a 0.5um filter patch and the round rubber- like injection port.
I have been regularly sealing these bags BEFORE putting them in the pressure cooker rather than after, i've had no problems with bursting bags. Its certainly easier to seal the bag before sterilizing it than after.
I always fold the bag along the pleat line from the bottom (where the substrate is) to the top, squeezing the air out along the way; then just make sure the open top end doesn't have any wrinkles in it before sealing it with an impulse sealer. I always seal it twice.
When I put it in the PC I loosely fold the top down but NOT fold it under the substrate cake. The filter patch should not be facing up but should be facing sideways. Thats to reduce the amount of water that may drip on to it from condensation on the lid. The patch WILL get a bit wet but it will dry out after the PC cycle is done. Best to let the PC cool down some (at least 4hr) after the cycle is done before opening it.
Also WBS is at least as good as millet, I just dont like too many sunflower seeds.
I'd like to try my bags this way, so my only question is....the myc will get enough air through the filter?
RR fills his bags up with air from his flowhood before he seals...so that's how I have always done it. But it's a huge pain in the ass to be honest.
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Asura
Cyantist


Registered: 08/01/11
Posts: 5,047
Loc: Texas
Last seen: 11 days, 17 hours
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Re: Official Copelandia/Panaeolus Thread [Re: space_shrooms]
#25685802 - 12/17/18 04:50 PM (5 years, 1 month ago) |
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Quote:
space_shrooms said: Asura your pans and trip reports are so awesome man awsome work
I sadly lost a grow to trich recently - had Hawaiians, goliaths and jams all going. I believe the error was my LC. I used unfiltered grain soak water. I think the sediment needed longer than the 20mins in the PC I gave it. All cultures looked clean on agar but then the grains ended up smelling like wet dust.
I’ve got pan. tropicalis colonizing well on grains now. I think i’m back on track. I’ll give all those other cultures another try again over the Christmas break too. The beauty of pans is that a few quarts of spawn provides enough shrooms for many trips.
Still tripping hard on the aussies from my last grow. These 1g+ trip reports sound terrifying but I’ll go there eventually. 0.75g gives me pure bliss and relaxation - very productive and revitalizing times, tucked away somewhere in the forest under a tree with the sun in my face. The visuals are incredible, a lot like DMT but with softer and rounder edges, while the pans are so relaxing and slightly sedating that even at the peak I often feel like I’m on the cusp of drifting off to sleep.
Maybe I need to try a lower dose of the pans...what you describe actually sounds quite nice
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mary fairchild
Pantheist


Registered: 12/06/14
Posts: 777
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Re: Official Copelandia/Panaeolus Thread [Re: Asura]
#25685965 - 12/17/18 06:15 PM (5 years, 1 month ago) |
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Quote:
Asura said:
Quote:
mary fairchild said: Just like to comment on what i've experienced with grow bags. I have been mostly using the ones that have a 0.5um filter patch and the round rubber- like injection port.
I have been regularly sealing these bags BEFORE putting them in the pressure cooker rather than after, i've had no problems with bursting bags. Its certainly easier to seal the bag before sterilizing it than after.
I always fold the bag along the pleat line from the bottom (where the substrate is) to the top, squeezing the air out along the way; then just make sure the open top end doesn't have any wrinkles in it before sealing it with an impulse sealer. I always seal it twice.
When I put it in the PC I loosely fold the top down but NOT fold it under the substrate cake. The filter patch should not be facing up but should be facing sideways. Thats to reduce the amount of water that may drip on to it from condensation on the lid. The patch WILL get a bit wet but it will dry out after the PC cycle is done. Best to let the PC cool down some (at least 4hr) after the cycle is done before opening it.
Also WBS is at least as good as millet, I just dont like too many sunflower seeds.
I'd like to try my bags this way, so my only question is....the myc will get enough air through the filter?
RR fills his bags up with air from his flowhood before he seals...so that's how I have always done it. But it's a huge pain in the ass to be honest.
So, I thought I read a while back that RR put tyvek sleeves in the bags then PC'd them without sealing them- to avoid bag rupture.
I dont do it that way. I've been sealing the bags before PC'ing them. Regarding pressure cooking, I haven't had any bags explode or form leaks from the pressure cooker. I always push out most of the air before sealing the bags- this way the 0.5 um filter patch wont be overwhelmed by air expansion.
The 0.5um filter patch has always provided enough gas exchange for mycelia to grow.
Filling the bag up with air from the flow hood before sealing, even though that air SHOULD be clean, isn't as safe to me as just sealing the bag before PC'ing.
Anyway the 0.5um filter patch has worked for me. They also sell bags with a 5.0um filter patch. This patch should give 10X the gas exchange as the 0.5 but theoretically some microbes can cross that barrier- they call the 5.0um filter "semi sterile". I ordered some of those bags and will see if they work for me without getting contams.
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Asura
Cyantist


Registered: 08/01/11
Posts: 5,047
Loc: Texas
Last seen: 11 days, 17 hours
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Ok thanks for the clarification. Mine are .2µ so I think I will stick with the tyvek sleeve method. Will get some .5's when I order again because I agree that pre-sealing would be better.
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Nimph
Im Unclecaptainblue!!!!

Registered: 08/12/18
Posts: 1,605
Loc: The dirty
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Re: Official Copelandia/Panaeolus Thread [Re: Asura]
#25685990 - 12/17/18 06:28 PM (5 years, 1 month ago) |
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Try meditating were you can hear the wind or something an meditate, im not a hippy or yoga enthusiast but i cant disagree with rainbows in my spine > .
Ill be sealing a bag with ducktape instead of zipties, ill let yall know how that goes.
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Asura
Cyantist


Registered: 08/01/11
Posts: 5,047
Loc: Texas
Last seen: 11 days, 17 hours
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Re: Official Copelandia/Panaeolus Thread [Re: Nimph]
#25686216 - 12/17/18 08:45 PM (5 years, 1 month ago) |
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Third and final flush. Picked a little early because I was seeing some spore drop and I wanted to try and get some prints for you guys.
This is it until January.
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mary fairchild
Pantheist


Registered: 12/06/14
Posts: 777
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Re: Official Copelandia/Panaeolus Thread [Re: Asura]
#25686359 - 12/17/18 09:51 PM (5 years, 1 month ago) |
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Great grow, thanks for sharing
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L0rdk4in
A noob


Registered: 05/12/14
Posts: 113
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Has anyone had any luck growing these on a straw or straw/grain bottle tek. Like in Pastywhyte invitro straw. I figure a pint pp5 would work if it has 2 inches of sub.
If it does work, are they aggressive enough that it would work with just MS alone. I am struggling to get much visible growth on agar I am thinking it might be worth a try.
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Brain Bulb
Insane in the Membrane



Registered: 11/09/17
Posts: 1,358
Last seen: 6 months, 23 days
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Re: Official Copelandia/Panaeolus Thread [Re: L0rdk4in]
#25686423 - 12/17/18 10:33 PM (5 years, 1 month ago) |
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@MaryFairchild @Asura,
Do you have a pic of your PC loaded with bag/s? About to start mine and want to make sure it’s set up right.
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Brain Bulb
Insane in the Membrane



Registered: 11/09/17
Posts: 1,358
Last seen: 6 months, 23 days
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Re: Official Copelandia/Panaeolus Thread [Re: Brain Bulb]
#25686433 - 12/17/18 10:39 PM (5 years, 1 month ago) |
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So my Tropicalis is going to have to wait for a bit. I don’t know why I even mess with MS syringes. I always end up with green mold... And it was just staring to look good:-(. Going to take it to agar to clean it up but that’s what I prefer. Only reason I got the MS in the first place was because I couldn’t find a print.. Back to the drawing board, but I have my Aussies, Alabama and Bisporus to keep me busy in the meantime 😊

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Nimph
Im Unclecaptainblue!!!!

Registered: 08/12/18
Posts: 1,605
Loc: The dirty
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Re: Official Copelandia/Panaeolus Thread [Re: Brain Bulb]
#25686743 - 12/18/18 04:51 AM (5 years, 1 month ago) |
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Thats just how pans are untill you clean them an are finicky in the early stages of cleaning ime. Thats my biggest hurdle to overcome is staying on top of the plates till t4 when stuff is usually juuust aboouut clean.
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Geinstein
Shroomery addict



Registered: 01/25/18
Posts: 1,784
Last seen: 2 years, 1 month
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Re: Official Copelandia/Panaeolus Thread [Re: Nimph]
#25686747 - 12/18/18 04:53 AM (5 years, 1 month ago) |
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Its been more then a week going for 2 and no pins What's the normal time for pans? Sorry first time growing these guys
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Nothing breads nothing
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Brain Bulb
Insane in the Membrane



Registered: 11/09/17
Posts: 1,358
Last seen: 6 months, 23 days
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Re: Official Copelandia/Panaeolus Thread [Re: Geinstein]
#25687153 - 12/18/18 10:29 AM (5 years, 1 month ago) |
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Quote:
Geinstein said: Its been more then a week going for 2 and no pins What's the normal time for pans? Sorry first time growing these guys
At 80F and saturating humidity about 5 days. I asked @ MaryFairchild the same question last night. If colder it will take longer. How are you fruiting and what is the temp/humidity?
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dmar


Registered: 09/09/18
Posts: 147
Loc: Earth, Milky Way, Virgo Superc...
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Re: Official Copelandia/Panaeolus Thread [Re: Brain Bulb]
#25687257 - 12/18/18 11:54 AM (5 years, 1 month ago) |
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I need help with my fruiting conditions. Sorry there's so many questions.
I have my 15 Qt sterlite tub with the lid flipped for over a week with no pins. Moisture seems good on the casing layer, some overgrowth but it looks healthy.
Is the flipped lid sufficient FAE for Pans? It has an odd lid, so it gives a pretty good size gap all around. Not just a tiny crack like on a shoebox or something.
I've had temp at 77-82 until last night I let it drop to about 73 to see if it would help me get some pins. Nothing yet.
I have a couple shoeboxes also in the same boat. I have them dub tubbed with binder clips, but offset the top a little to allow a little extra FAE.
I have a small heater going in the room to keep the temps right, so every half hour to every hour or so when that kicks on for a few minutes, that's my only airflow.
-Should I turn the ceiling fan on to increase FAE? -Should I adjust my temps? -Should I be misting if the casing layer seems moisturized or just let it be? -Should I be fanning several times per day? Currently I fan about once.
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mary fairchild
Pantheist


Registered: 12/06/14
Posts: 777
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Re: Official Copelandia/Panaeolus Thread [Re: dmar]
#25687800 - 12/18/18 04:27 PM (5 years, 1 month ago) |
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With Pans IMO genetics is the biggest factor in how well the grow works. Which species of Panaeolus are you trying?
The FAE question- If your flipped lid tub configuration works with cubes than it CAN work for Pans.
Temperature ~ 80F should work fine for Pans.
If the casing looks moist you don't have to mist.
In your setup I would be fanning several X per day but I dont usually grow Pans that way so maybe someone who does grow Pans in a tub would know better.
Time from casing to pinning varies between Panaeolus species- example bisporus takes ~8 to 10days, cyans & cambos ~6 days.
Edited by mary fairchild (12/18/18 04:41 PM)
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dmar


Registered: 09/09/18
Posts: 147
Loc: Earth, Milky Way, Virgo Superc...
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Quote:
mary fairchild said: With Pans IMO genetics is the biggest factor in how well the grow works. Which species of Panaeolus are you trying?
The FAE question- If your flipped lid tub configuration works with cubes than it CAN work for Pans.
Temperature ~ 80F should work fine for Pans.
If the casing looks moist you don't have to mist.
In your setup I would be fanning several X per day but I dont usually grow Pans that way so maybe someone who does grow Pans in a tub would know better.
Time from casing to pinning varies between Panaeolus species- example bisporus takes ~8 to 10days, cyans & cambos ~6 days.
Thanks, and I'm growing Pan Cyan Jamaicans right now, but I also have Cyan Australians that are just now being spawned. I spawned these on 12/9 and cased on about the 13th and moved into fruiting that day. So it's only been in fruiting for 5 days. That is the same lid configuration I use for my cubes, but I actually changed my lid setup slightly today. I flipped it back over and latched it, but I drilled 5 1/2" holes in the lid and put a single layer of MP tape over them. A hole on each corner and one in the middle. If anyone that grows in tubs could give me a bit of advice on the amount of FAE, that would be appreciated. Thanks guys. Hopefully I reach the finish line. All I need is a single mushroom so I can at least get a spore print
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dmar


Registered: 09/09/18
Posts: 147
Loc: Earth, Milky Way, Virgo Superc...
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Quote:
mary fairchild said: Ok, I hope I got this right- here's a batch the should almost provide enough coverage to case two 4qt. glass baking dishes:
350ml peat(well broken up) 350ml vermiculite 275ml dH2O mix it up well then get a pH reading
Add 1 teaspoon CaCO3-> mix well- let it sit for ~10 minutes, then get a pH reading.
The CaCO3 should bring the pH to somewhere between 7 and 7.5.
Here's the BIG CAVIAT- I've been determining the pH just using pH test strips- not very precise. IDK what a pH meter would say. I recently got a meter, just haven't tried it yet.
Also I really need to increase this recipe by~ 20% so I have a bit more than enough for two 4qt. trays
In regard to PH levels, I made a bunch of casing using peat/verm 50/50 yesterday and I put the casing layers on both my pans and my cubes. Well, I realized afterwards that I forgot to add calcium carbonate. I bought PH strips today and it tests at 6-6.5... Is there anything I can do to fix this now that the casing layer has already been applied? Could I mix calcium carb with distilled water and use it to mist and lower the surface PH that way? Or should I just make a little more and make it like 7.8 and sprinkle it on top? Or just leave it and pray. I dislike the latter.
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Psilosopherr
A psilly goose



Registered: 02/15/12
Posts: 12,278
Last seen: 1 month, 10 days
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Re: Official Copelandia/Panaeolus Thread [Re: dmar]
#25688090 - 12/18/18 06:36 PM (5 years, 1 month ago) |
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man they like 80 degrees? Its going to be a hot couple weeks, but I'll endure it for my babies.
I finally have a single clean pan cake so I'm here double checking some things. I see some people dunk pancakes and some don't. so maybe it works fine either way but I thought I'd ask what the people of this thread think works best.
do yall dunk?
edit: they're cambog if that matters
Edited by Psilosopherr (12/18/18 07:48 PM)
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dmar


Registered: 09/09/18
Posts: 147
Loc: Earth, Milky Way, Virgo Superc...
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Re: Official Copelandia/Panaeolus Thread [Re: Psilosopherr]
#25688103 - 12/18/18 06:45 PM (5 years, 1 month ago) |
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Quote:
Psilosopherr said: man they like 80 degrees? Its going to be a hot couple weeks, but I'll endure it for my babies. I see some people dunk pancakes and some don't.
do yall dunk?
I typically pour the syrup over my pancakes, then eat them with a fork. But to each their own.
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Asura
Cyantist


Registered: 08/01/11
Posts: 5,047
Loc: Texas
Last seen: 11 days, 17 hours
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Re: Official Copelandia/Panaeolus Thread [Re: Psilosopherr]
#25688130 - 12/18/18 06:59 PM (5 years, 1 month ago) |
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Poll y'all.
I have 32 prints I am prepping tonight. Some are really light...should I keep them or trash them? Only send out the dark prints? Or everything? Giving all the regulars here first choice before I take the rest to the marketplace.
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