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Brain Bulb
Insane in the Membrane



Registered: 11/09/17
Posts: 1,358
Last seen: 6 months, 24 days
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@MF. Looking at your grows and trying to figure what type of container/sub I should use. Just did A2G on 2 more bags so I’ll have 3 lbs of spawn.
I have a 33 qt. tub that’s roughly 24” L x 14” W x 6” deep. You think it would be wise to use all 3 bags in one shallow tub or should I do 3 smaller trays?
Off topic, but is there a substitute you would recommend for the ME in your agar recipe? Unfortunately I don’t have ME at the moment.
Thanks!
BB
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Perilous Chapel
Dialectical Satanist



Registered: 12/15/17
Posts: 66
Loc: Mormon Confederacy
Last seen: 3 years, 10 months
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Re: Official Copelandia/Panaeolus Thread [Re: Brain Bulb]
#25649681 - 11/30/18 11:14 PM (5 years, 1 month ago) |
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I’ve been having success with my cube varieties and I just did an agar to oat transfer of some Sandose Domesticate.....
Going to be doing some searching and reading of this thread.
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Asura
Cyantist


Registered: 08/01/11
Posts: 5,047
Loc: Texas
Last seen: 11 days, 18 hours
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Quote:
Perilous Chapel said: I’ve been having success with my cube varieties and I just did an agar to oat transfer of some Sandose Domesticate.....
Going to be doing some searching and reading of this thread.
My last attempt at pan cambo was a complete failure. Please post your results here!
With that said, does anyone have fruiting parameters for pan cambo? I tried to do them just like pan cyans and it was a complete flop.
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mary fairchild
Pantheist


Registered: 12/06/14
Posts: 777
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I always use ME agar brought up in horse poo tea. You definitely dont need the tea- but its just what I do. Someone else gave you a recipe that is pretty much what I would have suggested: So just bring it up in grain soak tea, maybe finely ground up herbivore poo. ~ 3g agar for 200ml tea.
I do trays rather than tubs; that way I could put them in a greenhouse and really define the humidity and fast air exchange. Tubs are cool and sometimes they work great. If you dont have a dialed in GH I dont know that a tray is going to be any better than a tub.
IDK- like I said Ive been partial to trays. If you choose the tray route- I'd consider taking one bag colonized grain to enough substrate to bring the total volume to ~3L. That volume would fit into a 4qt. glass baking dish nicely- giving you a volume 1.5 to 1.75 inches deep. So You cold do three of these trays- gives you plenty of surface area.
Or do the tub- all three grain spawn bags to enough substrate to give you around 2 inches depth.
Have you decided what substrate recipe you'll use?
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mary fairchild
Pantheist


Registered: 12/06/14
Posts: 777
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Re: Official Copelandia/Panaeolus Thread [Re: Brain Bulb]
#25649712 - 11/30/18 11:46 PM (5 years, 1 month ago) |
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Quote:
Brain Bulb said: @MF. Looking at your grows and trying to figure what type of container/sub I should use. Just did A2G on 2 more bags so I’ll have 3 lbs of spawn.
I have a 33 qt. tub that’s roughly 24” L x 14” W x 6” deep. You think it would be wise to use all 3 bags in one shallow tub or should I do 3 smaller trays?
Off topic, but is there a substitute you would recommend for the ME in your agar recipe? Unfortunately I don’t have ME at the moment.
Thanks!
BB
I always use ME agar brought up in horse poo tea. You definitely dont need the tea- but its just what I do. Someone else gave you a recipe that is pretty much what I would have suggested: So just bring it up in grain soak tea, maybe finely ground up herbivore poo. ~ 3g agar for 200ml tea.
I do trays rather than tubs; that way I could put them in a greenhouse and really define the humidity and fast air exchange. Tubs are cool and sometimes they work great. If you dont have a dialed in GH I dont know that a tray is going to be any better than a tub.
IDK- like I said Ive been partial to trays. If you choose the tray route- I'd consider taking one bag colonized grain to enough substrate to bring the total volume to ~3L. That volume would fit into a 4qt. glass baking dish nicely- giving you a volume 1.5 to 1.75 inches deep. So You cold do three of these trays- gives you plenty of surface area.
Or do the tub- all three grain spawn bags to enough substrate to give you around 2 inches depth.
Have you decided what substrate recipe you'll use?
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Brain Bulb
Insane in the Membrane



Registered: 11/09/17
Posts: 1,358
Last seen: 6 months, 24 days
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Quote:
mary fairchild said:
Quote:
Brain Bulb said: @MF. Looking at your grows and trying to figure what type of container/sub I should use. Just did A2G on 2 more bags so I’ll have 3 lbs of spawn.
I have a 33 qt. tub that’s roughly 24” L x 14” W x 6” deep. You think it would be wise to use all 3 bags in one shallow tub or should I do 3 smaller trays?
Off topic, but is there a substitute you would recommend for the ME in your agar recipe? Unfortunately I don’t have ME at the moment.
Thanks!
BB
I always use ME agar brought up in horse poo tea. You definitely dont need the tea- but its just what I do. Someone else gave you a recipe that is pretty much what I would have suggested: So just bring it up in grain soak tea, maybe finely ground up herbivore poo. ~ 3g agar for 200ml tea.
I do trays rather than tubs; that way I could put them in a greenhouse and really define the humidity and fast air exchange. Tubs are cool and sometimes they work great. If you dont have a dialed in GH I dont know that a tray is going to be any better than a tub.
IDK- like I said Ive been partial to trays. If you choose the tray route- I'd consider taking one bag colonized grain to enough substrate to bring the total volume to ~3L. That volume would fit into a 4qt. glass baking dish nicely- giving you a volume 1.5 to 1.75 inches deep. So You cold do three of these trays- gives you plenty of surface area.
Or do the tub- all three grain spawn bags to enough substrate to give you around 2 inches depth.
Have you decided what substrate recipe you'll use?
Since the first bag is a few days ahead of the other two I think I’ll try out trays and see how it goes. If all goes well with the first batch, I’ll stick with trays and try to dial things in when the other bags are ready.
Definitely open to recommendations as far as sub. Once the sub is colonized I’ll use a 1:1:1 peat/coir/verm recipe I saw in one of your posts as a casing. The room I use is set up at 80F with a RH that fluctuates between 40 - 50% (this is the ambient humidity). I’ll have to figure something out since these require more humidity.
In regards to your agar recipe. I made a batch of your poo tea but in place of the ME I used lite Caro syrup. Going to order some ME so I have it on hand if/when needed. Hopefully the caro syrup doesn’t mess it up.
I greatly appreciate your help! :-)
BB
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nube424


Registered: 12/03/17
Posts: 6,063
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Re: Official Copelandia/Panaeolus Thread [Re: Brain Bulb] 1
#25649993 - 12/01/18 06:39 AM (5 years, 1 month ago) |
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Is there a reason people use glass dishes with pans other than just for looks?
Im about to do my first pan grow soon. Pan bisporus. First time not growing cubes so it should be fun
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mary fairchild
Pantheist


Registered: 12/06/14
Posts: 777
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Re: Official Copelandia/Panaeolus Thread [Re: Brain Bulb]
#25650074 - 12/01/18 07:40 AM (5 years, 1 month ago) |
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Quote:
Brain Bulb said:
Quote:
mary fairchild said:
Quote:
Brain Bulb said: @MF. Looking at your grows and trying to figure what type of container/sub I should use. Just did A2G on 2 more bags so I’ll have 3 lbs of spawn.
I have a 33 qt. tub that’s roughly 24” L x 14” W x 6” deep. You think it would be wise to use all 3 bags in one shallow tub or should I do 3 smaller trays?
Off topic, but is there a substitute you would recommend for the ME in your agar recipe? Unfortunately I don’t have ME at the moment.
Thanks!
BB
I always use ME agar brought up in horse poo tea. You definitely dont need the tea- but its just what I do. Someone else gave you a recipe that is pretty much what I would have suggested: So just bring it up in grain soak tea, maybe finely ground up herbivore poo. ~ 3g agar for 200ml tea.
I do trays rather than tubs; that way I could put them in a greenhouse and really define the humidity and fast air exchange. Tubs are cool and sometimes they work great. If you dont have a dialed in GH I dont know that a tray is going to be any better than a tub.
IDK- like I said Ive been partial to trays. If you choose the tray route- I'd consider taking one bag colonized grain to enough substrate to bring the total volume to ~3L. That volume would fit into a 4qt. glass baking dish nicely- giving you a volume 1.5 to 1.75 inches deep. So You cold do three of these trays- gives you plenty of surface area.
Or do the tub- all three grain spawn bags to enough substrate to give you around 2 inches depth.
Have you decided what substrate recipe you'll use?
Since the first bag is a few days ahead of the other two I think I’ll try out trays and see how it goes. If all goes well with the first batch, I’ll stick with trays and try to dial things in when the other bags are ready.
Definitely open to recommendations as far as sub. Once the sub is colonized I’ll use a 1:1:1 peat/coir/verm recipe I saw in one of your posts as a casing. The room I use is set up at 80F with a RH that fluctuates between 40 - 50% (this is the ambient humidity). I’ll have to figure something out since these require more humidity.
In regards to your agar recipe. I made a batch of your poo tea but in place of the ME I used lite Caro syrup. Going to order some ME so I have it on hand if/when needed. Hopefully the caro syrup doesn’t mess it up.
I greatly appreciate your help! :-)
BB
I've had good luck with a substrate recipe : 70% hpoo, 20% coir, 10% verm brought to field capacity, and a 1:2 spawn to substrate ratio by volume. My ambient humidity is usually ~40% this time of year, I bump that up to ~80% with a wicking humidifier (Sunbeam brand from Walmart) but thats not enough so I also use an ultrasonic humidifier slaved to an Inkbird humidifier controller that I set up to 99%. Thats all done in a martha style greenhouse. You dont really need all this stuff. If you spray the surface of the case several times a day the resulting evaporation should bring the humidity just above the surface of the case to a high level. You should still try to find a way to bring your rooms humidity up.
As far as the Karo for your plate recipe- depending on how much you used I wouldn't do it that way- Karo is just a simple sugar- its too fast as a food source- If you just used a small amount, ~2% it's probably ok. Presence of the slower hpoo tea may mitigate.
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nube424


Registered: 12/03/17
Posts: 6,063
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So, my honey agar wont work for pans? Works great for cubes. Got a bisporus print on the way, i need to get my supplies in order
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mary fairchild
Pantheist


Registered: 12/06/14
Posts: 777
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Re: Official Copelandia/Panaeolus Thread [Re: nube424]
#25650082 - 12/01/18 07:43 AM (5 years, 1 month ago) |
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Quote:
nube424 said: Is there a reason people use glass dishes with pans other than just for looks?
Im about to do my first pan grow soon. Pan bisporus. First time not growing cubes so it should be fun 
Not really- I just like the sizes because they work well with the volumes of substrate I use.
Good luck!
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nube424


Registered: 12/03/17
Posts: 6,063
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Ohh ok. Thought it was to help hold moisture or something. They do look nice in glass. I have some pyrex bowls ill put to use.
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mary fairchild
Pantheist


Registered: 12/06/14
Posts: 777
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Re: Official Copelandia/Panaeolus Thread [Re: nube424]
#25650100 - 12/01/18 07:52 AM (5 years, 1 month ago) |
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Quote:
nube424 said: So, my honey agar wont work for pans? Works great for cubes. Got a bisporus print on the way, i need to get my supplies in order
Hey- if it works for cubes it may work for Pans as well- I dont know how much sugar you have in the recipe. To me it just doesn't feel right to use a simple sugar as the main food source. Those enzymes that are secreted from the mycelia are geared toward breaking down more complex substrates. I really don't know because i've never done it that way.
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nube424


Registered: 12/03/17
Posts: 6,063
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I use roughly 2%. Definitely not exact. But with cubes i get thick lc this way so i started using it as an agar recipe.
So for pans, a manure tea/grain soak water would be ideal? I just fuck up grain water agar, it always seems too nutritious no matter what dilution i try.
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mary fairchild
Pantheist


Registered: 12/06/14
Posts: 777
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Re: Official Copelandia/Panaeolus Thread [Re: nube424]
#25650114 - 12/01/18 08:02 AM (5 years, 1 month ago) |
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I always use malt extract agar- with or without manure tea- it works for me- so I haven't played much with agar recipes- I just suggested the grain soak water for plates in a pinch if malt extract isn't available.
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nube424


Registered: 12/03/17
Posts: 6,063
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Ok got it. Thanks mary. Congrats on the tag too
Ill be back in a few weeks with pics, and hopefully results.
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mullugh
Stranger

Registered: 03/26/18
Posts: 714
Last seen: 1 year, 7 months
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Re: Official Copelandia/Panaeolus Thread [Re: nube424]
#25650352 - 12/01/18 10:18 AM (5 years, 1 month ago) |
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Quote:
nube424 said: What did u do exactly? U mixed shit in ur grain spawn?
Abd why the fuckkkkkkkk is there rocks in there? Lol dude make proper grain spawn, sift ur manure, and cut it with something like coir or verm when u use cow manure or whatever that is.
It's a casing layer mate, cactus mix and verm. Yeah, I was experimenting by mixing manure in quite small quantity with the grain spawn, so it had flecks of manure in it. I would advise against it though.
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Brain Bulb
Insane in the Membrane



Registered: 11/09/17
Posts: 1,358
Last seen: 6 months, 24 days
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Re: Official Copelandia/Panaeolus Thread [Re: mullugh]
#25650644 - 12/01/18 12:58 PM (5 years, 1 month ago) |
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@MF
Okay cool. I’ll look into the wicking humidifier. Thanks!
I usually do 400 mL batches of agar which is enough for 20 plates. Since your recipe was for 300 mL I divided the ingredients by 3 and multiplied that by 4. Ended up using 400 mL distilled water ~ 6.4g agar powder but since I wasn’t sure on the Caro I used 10g which would be 2.5%. I’m ordering some ME so I won’t have to use the Caro again. Hopefully this batch will be okay.
With your sub recipe I should be okay with pasteurizing rather than PC’ing correct?
Thanks again!
BB
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mary fairchild
Pantheist


Registered: 12/06/14
Posts: 777
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Re: Official Copelandia/Panaeolus Thread [Re: Brain Bulb]
#25650736 - 12/01/18 01:55 PM (5 years, 1 month ago) |
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Yes- for a spawn to bulk style grow you are "supposed to pasteurize" the substrate. Typically I have done 2hr at 140F->160F.
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ElViajero
Traveler


Registered: 08/16/18
Posts: 295
Last seen: 2 years, 9 months
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Quote:
mary fairchild said: Ok, I hope I got this right- here's a batch the should almost provide enough coverage to case two 4qt. glass baking dishes:
350ml peat(well broken up) 350ml vermiculite 275ml dH2O mix it up well then get a pH reading
Add 1 teaspoon CaCO3-> mix well- let it sit for ~10 minutes, then get a pH reading.
The CaCO3 should bring the pH to somewhere between 7 and 7.5.
Here's the BIG CAVIAT- I've been determining the pH just using pH test strips- not very precise. IDK what a pH meter would say. I recently got a meter, just haven't tried it yet.
Also I really need to increase this recipe by~ 20% so I have a bit more than enough for two 4qt. trays
Have you ever had to adjust the ph, because the reading you got using ph test strips was off 7-7.5? im wondering how would you proceed in this case, how much CaCO3 would you add.
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Brain Bulb
Insane in the Membrane



Registered: 11/09/17
Posts: 1,358
Last seen: 6 months, 24 days
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Re: Official Copelandia/Panaeolus Thread [Re: ElViajero]
#25651034 - 12/01/18 04:35 PM (5 years, 1 month ago) |
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Aussie T5 transfer from yesterday recovered and taking off on the DFA recipe I’m trying!
Did two more Aussie A2G transfers as well. Agar recovered and starting to spawn in bags 2 & 3!
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