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Invisibledmar
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Registered: 09/09/18
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Re: Official Copelandia/Panaeolus Thread [Re: Asura]
    #25635184 - 11/23/18 08:16 PM (5 years, 2 months ago)

Quote:

Asura said:
Quote:

mary fairchild said:
I'm not clear on why one would use calcium hydroxide as well as calcium carbonate. Seems like the chance of messing it up goes way up if you use calcium hydroxide.

Just to keep it simple I just use calcium carbonate- very low solubility - just enough to bump pH to ~7.5.
IDK- these aren't cubes, maybe micro differences in pH of the case are important for Pans?? I wonder if BH has some thought on this?




I only tried calcium hydroxide because BH mentioned it. I messed it up because I used waaay too much. I had no
idea much a little would go a long way.

Basically, I am going through all of these "recipes" and testing them instead of just taking the ratios for
granted.

I am out of calcium carbonate. My latest casing is 1:1 peat/verm with distilled water. This is
4 cups peat, 4 cups verm and 3 cups distilled water. That's giving me a pH of 6.5. I am going to try and bump it
up with calcium hydroxide slowly to try and get to the ~7.8 range.



I'm following this closely, because i'm trying to get ready for how to spawn and case mine once my jars colonize. I was planning on using Hpoo+CVG with Calcium Carbonate. I haven't actually measured PH levels or anything, i've just been following TEKs with minor deviations. The peat should be relatively similar to my coir though, yeah? Also is distilled water better than normal water for the casing?


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Invisiblemary fairchild
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Re: Official Copelandia/Panaeolus Thread [Re: dmar]
    #25635214 - 11/23/18 08:40 PM (5 years, 2 months ago)

For the substrate- Hpoo +CVG- people dont usually try to adjust the pH of the substrate- the pH should be ~neutral. In other words you shouldn't need to add any calcium carbonate.

For the case- The case has peat moss as a component- peat will lower the pH of the case so to keep the pH at 7->8 you need to add calcium carbonate or calcium hydroxide.

Peat is not the same as coir. Coir should not affect the pH of the case while peat will definitely lower the pH.
Using distilled water vs non distilled- dH2O should give a pH~7. You dont need to use distilled water but dH2O should be just H2O- nothing else- so it erases variability that you could get if you used tap water, spring water etc.

I use dH2O when I make my case.


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OfflineAsuraS
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Re: Official Copelandia/Panaeolus Thread [Re: mary fairchild]
    #25635260 - 11/23/18 09:01 PM (5 years, 2 months ago)

Well, I will say this. I don't see any reason whatsoever to use calcium hydroxide. So I am not sure what
BH was doing.

I used a 1/2 tbsp. and it took 4qts peat/4qts verm from 6.5 pH to 9.9 pH. Holy SHIT, that is really strong.

I am ordering more calcium carbonate tomorrow so I need to wait to continue these experiments.


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Invisiblemary fairchild
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Re: Official Copelandia/Panaeolus Thread [Re: Asura] * 1
    #25635282 - 11/23/18 09:15 PM (5 years, 2 months ago)

For calcium carbonate - all I have is NOW brand from the health food store. For my purposes 12oz. will last forever.


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OfflineAsuraS
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Re: Official Copelandia/Panaeolus Thread [Re: mary fairchild]
    #25635292 - 11/23/18 09:25 PM (5 years, 2 months ago)

mary, can you clarify how much you are using for a batch of casing?


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Invisiblemary fairchild
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Re: Official Copelandia/Panaeolus Thread [Re: Asura] * 1
    #25635311 - 11/23/18 09:53 PM (5 years, 2 months ago)

Ok, I hope I got this right- here's a batch the should almost provide enough coverage to case  two 4qt. glass baking dishes:

350ml peat(well broken up)
350ml vermiculite
275ml dH2O
mix it up well then get a pH reading

Add 1 teaspoon CaCO3-> mix well- let it sit for ~10 minutes, then get a pH reading.

The CaCO3 should bring the pH to somewhere between 7 and 7.5.

Here's the BIG CAVIAT- I've been determining the pH just using pH test strips- not very precise. IDK what a pH meter would say. I recently got a meter, just haven't tried it yet.

Also I really need to increase this recipe by~ 20% so I have a bit more than enough for two 4qt. trays


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Invisiblewavyedge

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Re: Official Copelandia/Panaeolus Thread [Re: Asura]
    #25635431 - 11/24/18 12:02 AM (5 years, 2 months ago)

CaOH is only slightly soluble in water, maybe you could easily titrate it into the soil by creating a saturated solution and further diluting it. Further CaCO3 is generated when CO2 reacts with CaOH.


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OfflineFailboat
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Re: Official Copelandia/Panaeolus Thread [Re: wavyedge]
    #25635780 - 11/24/18 08:43 AM (5 years, 2 months ago)

It seems to me that aside from clean spawn a casing seems to be extremely effective with pans. Consensus?


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Offline99.99
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Re: Official Copelandia/Panaeolus Thread [Re: Failboat]
    #25637204 - 11/24/18 09:20 PM (5 years, 2 months ago)

I am still working on cleaning up some cultures from the Australian pan spore print I got last summer ,
I’m thinking this plate is perfect so I did a run of 10 more agar plates tonight, if they turn out in a week or so I’ll take it to grain



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OfflineBrain Bulb
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Re: Official Copelandia/Panaeolus Thread [Re: 99.99]
    #25637296 - 11/24/18 11:20 PM (5 years, 2 months ago)

Just found this thread and perfect timing!  I did a A2G transfer of some T4 PC Australia last night.  Finally got some plates w/o contaminants. 

This is my first time growing a Pan Cyan so any advice would be awesome.  I’ll have to back track through this thread to dig up some info as well.  Wish I would have seen this thread before I started my P. Cam Sandose.  Kind of stuck on those right now as well. 

Transferred the best plate to spawn but here are some pics of another plate.  Please let me know what you think and if I’m on the right track!

Thanks in advance!

BB

This is a T4 from a print I got on mushrooms.com a couple months ago.




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Offline99.99
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Re: Official Copelandia/Panaeolus Thread [Re: Brain Bulb]
    #25637345 - 11/25/18 12:10 AM (5 years, 2 months ago)

Your plates look perfect, MaryFairchild and bluehelix are the masters on this thread it would be wise to look at their individual grows and take their recommendations
From what I understand the cottony growth we both have on our plates is the best to fruit


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OfflineAsuraS
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Re: Official Copelandia/Panaeolus Thread [Re: 99.99]
    #25637391 - 11/25/18 01:21 AM (5 years, 2 months ago)

Wow, those are some nice plates, man. We will try to help.


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OfflineBrain Bulb
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Re: Official Copelandia/Panaeolus Thread [Re: Asura]
    #25638373 - 11/25/18 02:14 PM (5 years, 2 months ago)

Quote:

99.99 said:
Your plates look perfect, MaryFairchild and bluehelix are the masters on this thread it would be wise to look at their individual grows and take their recommendations
From what I understand the cottony growth we both have on our plates is the best to fruit





Awesome, thanks!  They have some amazing grows!

Quote:

Asura said:
Wow, those are some nice plates, man. We will try to help.




Thanks Asura!  I’ve only be cultivating for a few months so I’ll need all the help I can get.


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Offlinegt40
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Re: Official Copelandia/Panaeolus Thread [Re: Brain Bulb]
    #25639927 - 11/26/18 10:27 AM (5 years, 2 months ago)

Guys, after a couple of successful grows I have a damn problem which I can't understand.

Pans Jamaica
Each time after moving to jars colonization temps become slow and I start to see very little wet areas far from inoculation points. There are bacterial.
When you did MS it's obvious, but this time I've made a lot of dish transfers with super fast growth in it, clean plates but the result is the same.

I don't allow that it is rye prep problem. rinsing-soak 24h, cooking - stir 2h at 15psi... inoculated in same SAB as petri dishes transfers was made with 0 contamination rate. 


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InvisibleJHOVA
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Re: Official Copelandia/Panaeolus Thread [Re: gt40]
    #25640039 - 11/26/18 11:15 AM (5 years, 2 months ago)

Your agar pictures are over grown. Do you have pics of your agar before it reached the edges? We need more pics of your jars with better lighting.


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Offlinetryptkaloids
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Re: Official Copelandia/Panaeolus Thread [Re: JHOVA]
    #25640135 - 11/26/18 12:05 PM (5 years, 2 months ago)

I Guarantee it's in the prep. How sure are you its bacteria and not excess moisture? Dry it a bit more next time. Did you Shake your grain?


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"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
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Offlinegt40
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Re: Official Copelandia/Panaeolus Thread [Re: tryptkaloids]
    #25640603 - 11/26/18 03:45 PM (5 years, 2 months ago)

Quote:

tryptkaloids said:
I Guarantee it's in the prep. How sure are you its bacteria and not excess moisture? Dry it a bit more next time. Did you Shake your grain?



I think so because a few days after PC grains look very even, also growing temps are super slow which is a common problem indication, especially with pans.
My little petri dishes colonized completely for less than a week each time, but with all jars I have problems almost immediately.
I really can't grasp what's going wrong. 

suppose you agree that it's not about excess moisture.


Edited by gt40 (11/26/18 03:47 PM)


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InvisibleGerms
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Re: Official Copelandia/Panaeolus Thread [Re: gt40]
    #25640835 - 11/26/18 05:22 PM (5 years, 2 months ago)

Drop a wedge to LC and inoculate with that
LC worked great for me when I grew pans


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OfflineAsuraS
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Re: Official Copelandia/Panaeolus Thread [Re: Germs]
    #25640845 - 11/26/18 05:27 PM (5 years, 2 months ago)

:whathesaid:

I would do LC all the way. Agar to grain has never been anything but slow for me.


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OfflineBrain Bulb
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Re: Official Copelandia/Panaeolus Thread [Re: Asura]
    #25644035 - 11/28/18 12:39 AM (5 years, 2 months ago)

@mary fairchild

How much spawn are you using per 4qt baking dish?  I have a 1 lb bag of PC Australia spawning and want to know what to prepare for.  It’s starting to take off!



I also have two T4’s of the same species that are reacting differently on my current agar recipe. (Going to try something new on my next batch).  They both appear to have good healthy growth w/o contaminants, but one is bleaching or utilizing the food coloring I added and one isn’t.  Still new to cultivating and this is my first shot at a Pan so not sure what it means...


Here are some pics:


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