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Offlinemashxp
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Re: Official Copelandia/Panaeolus Thread [Re: Asura] * 1
    #25361202 - 08/01/18 10:14 PM (5 years, 5 months ago)

Has anyone ever seen this kind of aggressive Pan. Cyanescens mycelium growth?

It's creeping up all the way to the lit along the glass of the jars.



I used WBR mixed with a couple of spoons of dried cow manure as spawn. How do these jars look to you, more experienced growers? Is there something wrong with them?

I used a spores I took from a specimen I found in the wild (I live in a tropical SE-Asian country) and cleaned
them out on agar. Then transferred wedges to the grain jars (right) and used Munchauzen's Blenderless LI tek for some other jars. (left/middle) The left jar is likely to wet as I may have poured a bit too much LI into it.

My agar plates, that I left sitting around after being done transferring from them, have since shown the same behaviour like the jars. before they all looked proper and clean, like you would expect (sorry don't have pics).



It does look like mycelium to me, the way it grows... but I'm not sure what to make of this.


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Invisiblemary fairchild
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Registered: 12/06/14
Posts: 777
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Re: Official Copelandia/Panaeolus Thread [Re: mashxp]
    #25361298 - 08/02/18 12:30 AM (5 years, 5 months ago)

I've seen that behavior with Pan. cambo Thailand:


The blueing is another common trait of that variety; but maybe not relevant to your grow. Also Psilocybe mexicana Chicon Nindo can fill a whole petri dish with vertical growth.


Looks like mycelia to me- but your agar plates have really extensive growth, see where it leads.
Smell your spawn jars when you are ready to spawn to substrate. Make sure they smell like Pans- sweet almondy fragrance.
Also make sure you dont have any colors- just white.

I'm curious to see how your grow goes!


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InvisibleNumeroEno
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Re: Official Copelandia/Panaeolus Thread [Re: mary fairchild]
    #25361304 - 08/02/18 12:47 AM (5 years, 5 months ago)

I’m hopping on board with some freshly germinated pan bisporus.



--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

MY ELECTRIC INOCULATION LOOP


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Offlinemullugh
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Re: Official Copelandia/Panaeolus Thread [Re: NumeroEno]
    #25361334 - 08/02/18 01:41 AM (5 years, 5 months ago)

I've had to start over and throw out a ton of bacteria in jars. I made the mistake of using dirty LC and messed up a huge batch. One pan cyan jar remains, but when I first smelled it, it seemed a bit vinegary, but then came a smell of myc, so I've kept it to see what happens...I think it might be doing battle with bacteria.

Last night I inoculated 3 more jars of popcorn with agar wedges from what I think is a strong growth area of the Petri dish. Went back to popcorn as I was just sick of seeing all that infected rye...needed a change of scene.
Will update when I have something to share.


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OfflineDr. Psilocybin
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Re: Official Copelandia/Panaeolus Thread [Re: mashxp]
    #25361601 - 08/02/18 07:08 AM (5 years, 5 months ago)

Hey mashxp, I’m only on my first pan grow (cyans) but my agar plates sitting for 2-3 weeks have been doing the same thing. On some plates, the mycelium is going more slow and steady up the walls, and other plates have aggressive and desperate looking growth far up the wall like you have.


My 2-3 week old transfer plates have gone one step further and have started forming little dense white balls of growth everywhere. Has anyone experienced anything like this with pan cyans? I plan on taking a transfer of this growth to see how it does on a plate but I thought I’d see if anyone else had similar experience with this.

Quote:

Dr. Psilocybin said:

Puffballs scattered about, they started out very tiny and grew in size over time, most to the size of a pea:


However the ones on this plate got big:


It almost looks like hardened myc on the puffballs that are up against the glass. This is another angle of the big puffball plate.


More pics of puffballs up against the glass:











At first I thought the plates were maybe knotting/pinning, or that this was related to the aggressive casing-consuming growth, but at this point I’m not sure. Puffballs that are up against the glass are showing a different texture of mycelium that reminds me of sclerotia of some form which you can see in the pics. I think I remember reading that pan cyans were able to form tiny sclerotia, but I am not certain... Anyone know what’s going on here?


Just tiger dropped a p4 plate with nice mostly uniform growth to wbs 2 days ago which is recovering nicely. Just trying to make sure I’m not dealing with contams or a whole different species by mistake!


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Offlinemullugh
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Re: Official Copelandia/Panaeolus Thread [Re: Dr. Psilocybin]
    #25361797 - 08/02/18 09:15 AM (5 years, 5 months ago)

Apparently it is sclerotia. It's normal and I have it on mine too.


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Offlinemullugh
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Re: Official Copelandia/Panaeolus Thread [Re: mullugh]
    #25361805 - 08/02/18 09:17 AM (5 years, 5 months ago)

I wrote that before looking at the pictures..mine are much smaller. Anyone else with more experience care to help?


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OfflineAsuraS
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Re: Official Copelandia/Panaeolus Thread [Re: mullugh]
    #25362597 - 08/02/18 05:17 PM (5 years, 5 months ago)

Dr. Psilocybin, I would avoid the aggressive growth. You might think, "Wow, I bet that will be
great" but it's not. My experience is that anything that is aggressive on agar or in a jar will
lead to some intense overlay. I'm talking overlay like this bullshit right here:



I forget what mary looks for as far as phenotype's go, but me, I am looking for that nice wispy,
easy going myc.


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Invisiblemary fairchild
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Re: Official Copelandia/Panaeolus Thread [Re: Asura]
    #25362702 - 08/02/18 06:00 PM (5 years, 5 months ago)

As far as the Jams that have been circulating around the community- I'v seen two forms- the flatter form and the more cottony form neither are very wispy- at least not wispy like Pan bisporus:
Verses:  I was trying to link a phenotype on agar to a fruiting characteristic. NOW I DONT THINK I FOUND ONE.

This cottony form produced nice fruiting with very little overlay:


These two kind ratty looking colonies led to a good yield:

In this grow:
https://www.shroomery.org/forums/showflat.php/Number/24820407

I recently did a MULTISPORE grow of Jam that only produced overlay.

In other word I cant find any relationship between phenotype on agar and how well  these Jams fruit.
I've had beautiful looking colonies perform poorly and ratty looking colonies perform well.

I DO BELIEVE THAT THE PRINTS I GOT FROM MY LATEST JAM GROW ARE PRONE TO PRODUCING OVERLAY.

mf


Edited by mary fairchild (08/02/18 06:10 PM)


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OfflineDr. Psilocybin
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Re: Official Copelandia/Panaeolus Thread [Re: Asura]
    #25362737 - 08/02/18 06:15 PM (5 years, 5 months ago)

Asura, these are just my earlier transfer plates (P1 & P2 from P0 germ plate)that I have already taken transfers from and have just been sitting around doing nothing. For the P4 plate that I actually tiger dropped into my master wbs jar the other day, it’s growth was fairly uniform and moderate; neither super aggressive nor a slowpoke, neither super puffy nor super wispy and thin.

I just noticed the puffballs popping up out of nowhere within the last week on the old P1 & P2 plates and I’m just wondering what they might be out of boredom and curiosity. Since I already have my younger, prettier growth colonizing grains, transferring a puffball to another plate would just be for shits and giggles to see what happens. I look at the puffballs and think “Wtf are these”, so I took pics and asked here :grin:


Edited by Dr. Psilocybin (08/02/18 06:22 PM)


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OfflineAsuraS
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Re: Official Copelandia/Panaeolus Thread [Re: Dr. Psilocybin]
    #25364800 - 08/03/18 04:27 PM (5 years, 5 months ago)

Okay, so here's round 3...



I did a few things differently this time and I think it's working.

This is the same overly aggressive pan culture that I've been using. This time I let everything consolidate for 10 days. It looked ready at 5 days but I was like "nope, fuck you,
you need to settle down"

I cased with peat but I added a huge handful of CaCO3. The picture above is two days into fruiting.
No myc poking through, which I take as a good sign. Previously, all of my subs were overlayed
after two days.

Other than that, just added a temp controller and a heater. Even in this crazy Texas heat, I
think there are early morning hours where the FC was dropping to 70-72. I have the temp
controller set for 74-76.


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Invisiblemary fairchild
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Re: Official Copelandia/Panaeolus Thread [Re: Asura]
    #25365365 - 08/03/18 09:37 PM (5 years, 5 months ago)

Looks like maybe you've found a way to shut down that aggressive growth.

Do you know what the pH is? High pH should curb fungal growth- hopefully not enough to prevent pins forming.
Would be cool to see a side by side, with and without the extra CaCO3.
:popcorn:


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InvisibleNewbie8998

Registered: 06/22/18
Posts: 138
Re: Official Copelandia/Panaeolus Thread [Re: mary fairchild]
    #25365891 - 08/04/18 07:39 AM (5 years, 5 months ago)

First set of pins and knots on my panaeolus cyan’s last night excited to watch them grow!




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Invisiblemary fairchild
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Re: Official Copelandia/Panaeolus Thread [Re: Newbie8998]
    #25367136 - 08/04/18 07:55 PM (5 years, 5 months ago)

Congrats & good luck!


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InvisibleNewbie8998

Registered: 06/22/18
Posts: 138
Re: Official Copelandia/Panaeolus Thread [Re: mary fairchild]
    #25367603 - 08/05/18 05:50 AM (5 years, 5 months ago)

Hey Mary couple questions, my pins seemed to stall they haven’t really done anything after 24 hours and now I’m getting a lot of side pins. I read that you had said the cyans are really finicky and that you shouldn’t mist them and I haven’t but the sides of my tub have good moisture and standing water droplets. What do you think I should do in order to get the top of the substrate to pin and grow? I’m using the shoebox method and have 2 of them both doing the same thing. Or would it just be that I need to wait? I’ve been using a 12on 12off light schedule and I rotate my lids 90 degrees for better FAE.




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Invisiblemary fairchild
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Re: Official Copelandia/Panaeolus Thread [Re: Newbie8998]
    #25367931 - 08/05/18 09:56 AM (5 years, 5 months ago)

Do you have a case layer over your substrate? If not, you're more likely to have problems.

Give it another 24hr period.

Some varieties grow at a slower rate than others. The third picture shows  opening of the cap at an early stage, ie. while the mushroom is still really small.  Normally I'd think to water it with syringe but it looks moist anyway. If all you're shrooms caps start to open while they are still that small, something is wrong with the conditions.

As long as the case layer doesn't look like its starting to dry I wouldn't water it. If case is starting to dry you can water the dry areas with a syringe and needle.

If you don't see growth by 48hrs then something is wrong, probably with the environmental conditions.


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InvisibleNewbie8998

Registered: 06/22/18
Posts: 138
Re: Official Copelandia/Panaeolus Thread [Re: mary fairchild]
    #25367967 - 08/05/18 10:16 AM (5 years, 5 months ago)

Ok thank you so much, I did add a casing to both and it may be dry I will try watering it. In your experience what would be the optimal environmental conditions this strain does well in? I have my house at a constant 73-78 degrees


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Invisiblemary fairchild
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Re: Official Copelandia/Panaeolus Thread [Re: Newbie8998]
    #25368110 - 08/05/18 12:05 PM (5 years, 5 months ago)

What variety is it?
Some varieties like it warmer than that. Regardless of variety I fruit at 78-83F.


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InvisibleNewbie8998

Registered: 06/22/18
Posts: 138
Re: Official Copelandia/Panaeolus Thread [Re: mary fairchild]
    #25368157 - 08/05/18 12:37 PM (5 years, 5 months ago)

Panaeolus Cyanescens it was a syringe from a vendor


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Invisiblemary fairchild
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Re: Official Copelandia/Panaeolus Thread [Re: Newbie8998]
    #25368237 - 08/05/18 01:27 PM (5 years, 5 months ago)

Did they not tell you what variety of Pan. cyan?
For example there is Pan cyan Jamaica or Hawaii or Australia, etc. The different varieties are really just strains that were originally collected at different places. There can be a huge variation in behaviors within a species.

Some Pans will not do well if temperature is much below 80F.

Regardless, its kinda late to change things; give it another 24-48hrs. If they start to turn black they are rotting.


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