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FishLevelMidnight
Aquaman



Registered: 09/01/17
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Re: Official Copelandia/Panaeolus Thread [Re: pixelpopper]
#25322376 - 07/12/18 01:36 PM (5 years, 6 months ago) |
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I’ve used black kow for pans and got fruits 1:1:1 with coir:Verm:black kow and ~7% gypsum
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pixelpopper
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Thanks for linking that post! I hadn't seen it yet. And from RR even.. good to know
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pixelpopper
Crap Artist


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Quote:
Dr. Freeman said: Found it: Black kow will work for pans.
Thanks for linking that post! I hadn't seen it yet. And from RR even.. good to know
Quote:
fishermansjc said: I’ve used black kow for pans and got fruits 1:1:1 with coir:Verm:black kow and ~7% gypsum
Thanks for the specifics on recipe mix. Maybe I will try black kow unless I can find the real shit
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Germs
Space Force


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Re: Official Copelandia/Panaeolus Thread [Re: pixelpopper]
#25322422 - 07/12/18 02:01 PM (5 years, 6 months ago) |
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I had decent success using Earthgro organic manure and humus. 40 lb bag is like $2.50 at home depot. Its like mud when hydrated so gotta mix with verm 1:1, adding some coir could help too. I tried both with an without
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pixelpopper
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Re: Official Copelandia/Panaeolus Thread [Re: Germs]
#25322444 - 07/12/18 02:14 PM (5 years, 6 months ago) |
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Thanks Germs. I've definitely got local access to Earthgro
I've still got a huge bag of verm I haven't used so maybe I will save it for this.
The verm is enough to make it not so thick/muddy? Seems like it would still be pretty thick with just verm
I like fishermansjc's mix adding verm & coir
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mary fairchild
Pantheist


Registered: 12/06/14
Posts: 777
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Re: Official Copelandia/Panaeolus Thread [Re: Dr. Freeman]
#25322474 - 07/12/18 02:41 PM (5 years, 6 months ago) |
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Quote:
Dr. Freeman said: Hello guys. I have a question regarding Pans on agar. I have some agar plates with Panaeolus cambodginiensis and I want to know if it looks normal. I understand pans have tomentose mycelium, what I have isn't really rhizomorphic but close.
 This dish is contaminated, so I just took the lid of for the picture.
Hi Dr. Freeman, Thats Rhizomorph -not normal, how many passages from the germination plate?
That looks like an early passage of cubensis mycelia- ie. rhizomorphic form overriding undifferentiated background. Sometimes vendors mislabel spore syringes.
Ive seen Pans show some hints of rhizomorphic form but never to that degree. If you are sure its Panaeolus, its an unusual variant.
Maybe isolate the rhizomorphic form on agar and take it to fruiting; see what you get.
Edited by mary fairchild (07/12/18 03:02 PM)
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FishLevelMidnight
Aquaman



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Re: Official Copelandia/Panaeolus Thread [Re: pixelpopper]
#25322566 - 07/12/18 03:34 PM (5 years, 6 months ago) |
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Quote:
pixelpopper said: Thanks Germs. I've definitely got local access to Earthgro
I've still got a huge bag of verm I haven't used so maybe I will save it for this.
The verm is enough to make it not so thick/muddy? Seems like it would still be pretty thick with just verm
I like fishermansjc's mix adding verm & coir
I've done that mix (volumetric) for all of my bulk substrates and it performs well and goes 3-5 flushes (if space allows) with no issues. Breaks down nice and quick in the compost too.
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Dr. Freeman
An agent of Order



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Re: Official Copelandia/Panaeolus Thread [Re: mary fairchild]
#25322794 - 07/12/18 06:02 PM (5 years, 6 months ago) |
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Quote:
mary fairchild said:
Quote:
Dr. Freeman said:

Hi Dr. Freeman, Thats Rhizomorph -not normal, how many passages from the germination plate?
That looks like an early passage of cubensis mycelia- ie. rhizomorphic form overriding undifferentiated background. Sometimes vendors mislabel spore syringes.
Ive seen Pans show some hints of rhizomorphic form but never to that degree. If you are sure its Panaeolus, its an unusual variant.
Maybe isolate the rhizomorphic form on agar and take it to fruiting; see what you get.
Mi Mary. This is a first transfer. The spores came from a very small print, the size of a regular pan, so I think it could be the real stuff. I have other dishes showing the puffy tomentose mycelium that is so characteristic of pans, growing along this rhizo-like mycelium. Upon closer inspection it really doesn't looks like the rhizomorphic mycelium of cubes.

I have rhizo-like mycelium, flat tomentose (thinner) and puffy tomentose(thicker). I plan on isolating each phenotype tomorrow. This post made me decide to test out both the thick and thin phenotype: Assessing overlay in two Panaeolus cyanescens Jamaican
Post edit I have already isolated the thinner phenotype via needle point biopsy on my first transfer from the germination plate, so that one is safe, just need to spread it to a few more dishes for safekeeping.
Edited by Dr. Freeman (07/12/18 06:15 PM)
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mary fairchild
Pantheist


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Re: Official Copelandia/Panaeolus Thread [Re: Dr. Freeman]
#25322851 - 07/12/18 06:44 PM (5 years, 6 months ago) |
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The 2nd picture looks easily like cambo Sandos; but the first...
I'd like to see that rhizo form isolated on a plate. Very interesting to see how that form fruits. Good luck!
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Dr. Freeman
An agent of Order



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Re: Official Copelandia/Panaeolus Thread [Re: mary fairchild]
#25322938 - 07/12/18 07:23 PM (5 years, 6 months ago) |
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Quote:
mary fairchild said: The 2nd picture looks easily like cambo Sandos; but the first...
I'd like to see that rhizo form isolated on a plate. Very interesting to see how that form fruits. Good luck!

Thanks. I hope I'll see some fruits too very soon. The first picture also looks like cambo when taken from the right angle, exept for the rhizo part. The second also has a very rhizo-like growth from 3 to 9 o clock. And, I have this one:
 It's very cambo-like exept for the part I took a picture of. I have a few dishes with this mixed growth patern. All came from the same donor plate where I germinated the spores.
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Nobler Hino
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Re: Official Copelandia/Panaeolus Thread [Re: Dr. Freeman] 1
#25323001 - 07/12/18 07:46 PM (5 years, 6 months ago) |
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cambo goliath plates looking great after first transfers from ms. the fuzzy half looks suspect to me though.
 might even be a cube mixup from vendor.
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Edited by Nobler Hino (07/12/18 07:52 PM)
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tryptkaloids
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Re: Official Copelandia/Panaeolus Thread [Re: Nobler Hino]
#25323022 - 07/12/18 07:59 PM (5 years, 6 months ago) |
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Damn. I wonder if its mislabeling or cross contamination
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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mary fairchild
Pantheist


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Re: Official Copelandia/Panaeolus Thread [Re: Nobler Hino] 1
#25323033 - 07/12/18 08:02 PM (5 years, 6 months ago) |
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Validation,
I've never seen Goliath- or any other Pan for that matter- look like that. Looks like classic cubensis. This is what Goliath looks like- very cottony:
 
Lets see how it fruits.
Edited by mary fairchild (07/12/18 09:12 PM)
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Germs
Space Force


Registered: 06/26/11
Posts: 4,607
Loc: Texas
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Re: Official Copelandia/Panaeolus Thread [Re: mary fairchild]
#25323039 - 07/12/18 08:05 PM (5 years, 6 months ago) |
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Quote:
mary fairchild said: Validation,
I've never seen Goliath- or any other Pan for that matter- look like that:
 
Lets see how it fruits.
That’s how mine looked 
I have a qt jar of pan cambo almost ready to spawn that is bluing in a small area, could be bacteria or unseen contamination? Master Mary?
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tryptkaloids
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Re: Official Copelandia/Panaeolus Thread [Re: Germs]
#25323049 - 07/12/18 08:09 PM (5 years, 6 months ago) |
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Could it be too dry? Im sure you know your grain prep, just spitballing
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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tryptkaloids
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Re: Official Copelandia/Panaeolus Thread [Re: tryptkaloids]
#25323051 - 07/12/18 08:09 PM (5 years, 6 months ago) |
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Im pretty sure cambo is one of the ones that likes to put out small sclerotia
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Germs
Space Force


Registered: 06/26/11
Posts: 4,607
Loc: Texas
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Re: Official Copelandia/Panaeolus Thread [Re: Germs]
#25323057 - 07/12/18 08:13 PM (5 years, 6 months ago) |
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 Here’s what it looks like, kinda hard to see it, upper right. I have a hard time telling subtle colors apart
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mary fairchild
Pantheist


Registered: 12/06/14
Posts: 777
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Re: Official Copelandia/Panaeolus Thread [Re: Germs]
#25323059 - 07/12/18 08:14 PM (5 years, 6 months ago) |
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Goliath likes to blue int the grain jar- it always seems to do that. Psilocin oxidizing when it leaks out of old mycelia?
 So thats standard behavior for Goliath-but the rhizo form is not- hmm.
Edited by mary fairchild (07/12/18 08:58 PM)
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Germs
Space Force


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Posts: 4,607
Loc: Texas
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Re: Official Copelandia/Panaeolus Thread [Re: tryptkaloids]
#25323060 - 07/12/18 08:15 PM (5 years, 6 months ago) |
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Quote:
tryptkaloids said: Could it be too dry? Im sure you know your grain prep, just spitballing 
I did these with LI and was just freeballin it lmao i think it may have threw some of the jars off That’s why sometimes I like the extra step of putting it in a syringe
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Germs
Space Force


Registered: 06/26/11
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Loc: Texas
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Re: Official Copelandia/Panaeolus Thread [Re: Germs]
#25323067 - 07/12/18 08:17 PM (5 years, 6 months ago) |
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 A couple other jars These three are the furthest along I feel should be about ready. Some areas have wispy myc though
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