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Invisiblemary fairchild
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Re: Official Copelandia/Panaeolus Thread [Re: Humble Newcomer]
    #25126780 - 04/09/18 07:35 PM (5 years, 9 months ago)

Quote:

Humble Newcomer said:
Very true. Way higher chance of having a contammed syringe for that reason. But was that ever a question? That's not what's being voted is it, that's obvious.

On the one hand it is another contamination vector, on the other it makes long term storage much friendlier and much more likely to revive. If u want long long term storage of your spores, a clean syringe in a dark bag in the fridge can last decades.

I read some people have used much older syringes than I'm using and they worked fine, I seem to remember the 20yr number. If done right it's basically the same sterile water storage that the advanced guys use to store their live cultures for 7+ years. Even there, there's a guy who revived his live wedge after 20 years of room temp sterile water storage.

But your getting into lab equip and technique I simply don't have.

At the end of the day, there's no difference in strain creation capability and syringe offers 3x + as long of storage . But who made it, do u have agar, do u have time, well...

So it's completely a case by case basis

Edit : I'm sorry that seems rude in general I think I just misunderstood, if it's a personal preference vote them yeah I guess print all the way prob for everyone as soon as they get into agar.

Though I've been floored with the differences I've been noticing I'm 5yr print vs 5yr syringe in germination, a ten fold difference at five years I basically know that at year 6 or 7 I'll have a zero percent germination rate from print, even applying on really wet agar and leaving two weeks. And I notice no degradation at five years in syringe due yo spore hydration and i read of accounts of people using ten yr plus old syringe. So i thought I'd share.apologies





Thats what it is for me- either the gosh darn syringe is contaminated or nothing grows. Otherwise they're ok.


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Offlinehamloaf
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Re: Official Copelandia/Panaeolus Thread [Re: mary fairchild] * 1
    #25127245 - 04/09/18 11:18 PM (5 years, 9 months ago)

So far, so good.  :tensegrin:
 


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How I Get Stuff done. - My Reference Guide. - My Grows.


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Invisiblemary fairchild
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Re: Official Copelandia/Panaeolus Thread [Re: hamloaf]
    #25127319 - 04/10/18 12:13 AM (5 years, 9 months ago)

Looks spot on at this point!

The jars-they look like you poked the needle in several points - the classic tek.
Did you just open the plastic lid to inoculate?


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Invisiblebacillus
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Re: Official Copelandia/Panaeolus Thread [Re: Humble Newcomer]
    #25127460 - 04/10/18 03:26 AM (5 years, 9 months ago)

Quote:

Humble Newcomer said:
Though I've been floored with the differences I've been noticing I'm 5yr print vs 5yr syringe in germination, a ten fold difference at five years I basically know that at year 6 or 7 I'll have a zero percent germination rate from print, even applying on really wet agar and leaving two weeks. And I notice no degradation at five years in syringe due yo spore hydration and i read of accounts of people using ten yr plus old syringe. So i thought I'd share.apologies




1. Two weeks is not long. I waited a month for serbica and semilanceata.
2. How about making a syringe from that old print to hydrate the spores?


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Mycelium on agarLet's trade


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Offlinehamloaf
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Re: Official Copelandia/Panaeolus Thread [Re: mary fairchild]
    #25127659 - 04/10/18 07:05 AM (5 years, 9 months ago)

Quote:

mary fairchild said:
Looks spot on at this point!

The jars-they look like you poked the needle in several points - the classic tek.
Did you just open the plastic lid to inoculate?



Hi, mary.  Thanks!  You are correct.  Lids were opened in order to inoculate (flowhood), and inoculated at several points.  Not sure how multiples of one innoc point were created.  Large chunks of culture came out of syringe, and settled on top of substrate at each innoc point, so I bet two streams of liquid running down substrate per innoc point was created.  The bags were inoculated in a 360 degree, sweeping motion.


--------------------

   
How I Get Stuff done. - My Reference Guide. - My Grows.


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InvisibleGerms
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Re: Official Copelandia/Panaeolus Thread [Re: hamloaf]
    #25127675 - 04/10/18 07:13 AM (5 years, 9 months ago)

Quote:

hamloaf said:
So far, so good.  :tensegrin:
 



:solidnod:


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InvisibleHumble Newcomer
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Re: Official Copelandia/Panaeolus Thread [Re: bacillus]
    #25129392 - 04/10/18 07:42 PM (5 years, 9 months ago)

Quote:

bacillus said:

2. How about making a syringe from that old print to hydrate the spores?




That's exactly what I was thinking of doing with the remainder of the print I was using, make a syringe and let it sit or might as well put a weight on the syringe while waiting, I kind of agree with trypt, how much will having external water pressure pushing on the spores cell walls help it hydrate, but I might as well try it too. Just sitting in the syringe for a few days would hopefully be a huge difference.

Luckily when using 100x more spores than usual, a literal clump of black on the innoc loop, I finally got some growth ins everal plates. It consumed my whoke print, but hey, I got three plates of yhick growth now.

With my luck I'd find that making a syringe with the old print wouldn't help anyway. Maybe if rehydration was possible it should have done it over two weeks on wet agar. Maybe if u let it get too dry there's no rehydration possible whatsoever :shrug:

From what I understand that's the only reason spores aren't viable forever;moisture


--------------------
How I made my mush GH


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InvisiblemushboyMDiscord
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Re: Official Copelandia/Panaeolus Thread [Re: Humble Newcomer] * 2
    #25129405 - 04/10/18 07:47 PM (5 years, 9 months ago)

my 2nd pan fruit. growing off a moldy clone dish.


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InvisibleHumble Newcomer
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Re: Official Copelandia/Panaeolus Thread [Re: Humble Newcomer]
    #25129411 - 04/10/18 07:50 PM (5 years, 9 months ago)

Bacillus - I'm sorry It didn't click at first, you're saying it took a solid month for a spore to germinate for those species? How old was print?

Interesting. I asked myself why was I in such a rush then and i remembered I had tiny orange specks of bacterial growth show up around day 7 and start to get a little big by day 13 or 15



Has anyone else needed to wait so long?


--------------------
How I made my mush GH


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InvisibleJosex
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Re: Official Copelandia/Panaeolus Thread [Re: mushboy]
    #25129413 - 04/10/18 07:51 PM (5 years, 9 months ago)

Quote:

mushboy said:
my 2nd pan fruit. growing off a moldy clone dish.




:snoopyes:


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OfflineAsuraS
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Re: Official Copelandia/Panaeolus Thread [Re: mushboy]
    #25129430 - 04/10/18 07:55 PM (5 years, 9 months ago)

Quote:

mushboy said:
my 2nd pan fruit. growing off a moldy clone dish.





I mean how many projects does one have to have going to have something like this happen? I'm
imagining your grow room is like that warehouse scene at the end of Indiana Jones.


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InvisibleGerms
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Re: Official Copelandia/Panaeolus Thread [Re: Josex]
    #25129431 - 04/10/18 07:56 PM (5 years, 9 months ago)

Just put some pan trop to LC with the JoSEX poke
:rockon:


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InvisiblemushboyMDiscord
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Re: Official Copelandia/Panaeolus Thread [Re: Asura]
    #25129478 - 04/10/18 08:12 PM (5 years, 9 months ago)

Quote:

Asura said:
Quote:

mushboy said:
my 2nd pan fruit. growing off a moldy clone dish.





I mean how many projects does one have to have going to have something like this happen? I'm
imagining your grow room is like that warehouse scene at the end of Indiana Jones.




to be honest thats the original donor plate(out of two). the other one is being utilize.
if you look closely it almost looks like the pin is growing out of the bruised tissue.

it was in the bottom of my trashcan.


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InvisibleHumble Newcomer
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Re: Official Copelandia/Panaeolus Thread [Re: mushboy]
    #25129526 - 04/10/18 08:29 PM (5 years, 9 months ago)

I think that's super neat. I haven't had anything pin on agar yet.


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InvisiblemushboyMDiscord
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Re: Official Copelandia/Panaeolus Thread [Re: Humble Newcomer]
    #25129530 - 04/10/18 08:31 PM (5 years, 9 months ago)

ive had pan knots but not agar pins. im stoked its from a clone culture.


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OfflineDoc9151M
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Re: Official Copelandia/Panaeolus Thread [Re: Humble Newcomer]
    #25129716 - 04/10/18 09:44 PM (5 years, 9 months ago)

Quote:

Humble Newcomer said:
Quote:

bacillus said:

2. How about making a syringe from that old print to hydrate the spores?




That's exactly what I was thinking of doing with the remainder of the print I was using, make a syringe and let it sit or might as well put a weight on the syringe while waiting, I kind of agree with trypt, how much will having external water pressure pushing on the spores cell walls help it hydrate, but I might as well try it too. Just sitting in the syringe for a few days would hopefully be a huge difference.

Luckily when using 100x more spores than usual, a literal clump of black on the innoc loop, I finally got some growth ins everal plates. It consumed my whoke print, but hey, I got three plates of yhick growth now.

With my luck I'd find that making a syringe with the old print wouldn't help anyway. Maybe if rehydration was possible it should have done it over two weeks on wet agar. Maybe if u let it get too dry there's no rehydration possible whatsoever :shrug:

From what I understand that's the only reason spores aren't viable forever;moisture




They should be fully hydrated after 24hrs in the syringe .


--------------------


Psilocybe cubensis data collection thread. please help with this project if you hunt wild cubensis.
https://www.shroomery.org/forums/showflat.php?Cat=0&Number=26513593&page=0&vc=1#26513593


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Invisiblebacillus
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Re: Official Copelandia/Panaeolus Thread [Re: Doc9151]
    #25130135 - 04/11/18 03:50 AM (5 years, 9 months ago)

One of the pinning triggers is contamination. Cultures pin as a way of defending theirselves. Particularly from bacteria, maybe from mold too?

@Humble Newcomer: Both species are rather slow/difficult. The prints were fresh, one 100%, the other probably. The plates were clean, but some slow growing bacteria are not a problem for me.


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InvisibleHumble Newcomer
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Re: Official Copelandia/Panaeolus Thread [Re: bacillus]
    #25130447 - 04/11/18 09:09 AM (5 years, 9 months ago)

Fresh prints and still took that long wow.

Yeah In that pic those little bacterial dots I'm pretty sure they were picked up in the print because of how they were spread out and not near the edges, they got pretty big within another week but luckily I noticed two tiny pan myc spots and was able to wait until they got bigger and cut away



But if it had taken much longer than that two weeks to get pan myc I would have been out of luck.

Ime fresh spores fire off in four days or so, old up to two weeks, it's a stark difference. I'll have to make sure I wait a month from now on tho, thanks


--------------------
How I made my mush GH


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InvisibleMycolorado
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Re: Official Copelandia/Panaeolus Thread [Re: Humble Newcomer]
    #25130548 - 04/11/18 10:10 AM (5 years, 9 months ago)

I have some fresh-ish prints with some stubborn spores (cyan Jam and Goliath), however, they're on some hard-ass agar...making up some super soft poo agar to try them on, though the Goliath did finally start popping after about 8 or 9 days give or take.


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InvisibleTheMadHatter420
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Re: Official Copelandia/Panaeolus Thread [Re: mushboy]
    #25130552 - 04/11/18 10:12 AM (5 years, 9 months ago)

Quote:

mushboy said:
ive had pan knots but not agar pins. im stoked its from a clone culture.




Throw a casing on the plate and watch it fruit small fruits.

(pan. cambo)




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JOIN THE POW WOW


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