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Offlinecube talk
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Re: Official Copelandia/Panaeolus Thread [Re: Asura]
    #25123631 - 04/08/18 03:04 PM (5 years, 9 months ago)

Quote:

Asura said:
Cube talk, have you seen MacMerdin's Simple Pan-Cakes?

https://www.shroomery.org/forums/showflat.php/Number/18357343#18357343




I read it

problem is the same question I had here, someone help me wrap my head around the point of burying the jars?


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InvisibleHumble Newcomer
Diddler de niños
I'm a teapot


Registered: 03/12/17
Posts: 1,483
Re: Official Copelandia/Panaeolus Thread [Re: cube talk]
    #25123649 - 04/08/18 03:13 PM (5 years, 9 months ago)

I think the idea was to get the humidity closer to the top of the cake to induce pinning earlier. Instead of the bottom of your pf cake sitting in the microclimate produced at the surface, the top of your cake is. the whole inside of a SGFC is humid, but right at the surface of the perlite is better than 3" up is the thought.

Some people also have bad luck getting wet perlite chunks onto the lid or whatever they're using to elevate a standard PF cake on top of the perlite, this makes contact with cake, sometimes unnoticed until its created a contamination like green spots on your cake bottom.

Lots of variations out there. Find what works for you


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How I made my mush GH


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Invisiblemary fairchild
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Registered: 12/06/14
Posts: 777
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Re: Official Copelandia/Panaeolus Thread [Re: cube talk]
    #25123899 - 04/08/18 05:04 PM (5 years, 9 months ago)

Quote:

cube talk said:
Quote:

Asura said:
Cube talk, have you seen MacMerdin's Simple Pan-Cakes?

https://www.shroomery.org/forums/showflat.php/Number/18357343#18357343




I read it

problem is the same question I had here, someone help me wrap my head around the point of burying the jars?




Burying the jars in perlite means, rather than just setting the jars down on the perlite in your shotgun fruiting chamber, sink them down into the perlite so that the rim of the jars is ~even with the perlite surface.
You don't have to do it, but I would. The relative humidity should rise the closer you get to the perlite surface because you are closer to the point of evaporation.

"MacMerdin Pancakes" - or "PF tek for Pans" - is a good small scale way to grow Pans. The method works best if the jars containing the substrate are inoculated with a water suspension of either clean spores or mycelia.


Edited by mary fairchild (04/09/18 03:29 AM)


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Offlinecube talk
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Re: Official Copelandia/Panaeolus Thread [Re: mary fairchild] * 1
    #25124780 - 04/09/18 01:56 AM (5 years, 9 months ago)

Quote:

mary fairchild said:
Quote:

cube talk said:
Quote:

Asura said:
Cube talk, have you seen MacMerdin's Simple Pan-Cakes?

https://www.shroomery.org/forums/showflat.php/Number/18357343#18357343




I read it

problem is the same question I had here, someone help me wrap my head around the point of burying the jars?




Burying the jars in perlite means, rather than just setting the jars down on the perlite in your shotgun fruiting chamber, sink them down into the perlite so that the rim of the jars is ~even with the perlite surface.
You don't have to do it, but I would. The relative humidity should rise the closer you get to the perlite surface because you are closer to the point of evaporation.

"MacMerdin Pancakes" - or "PF tek for Pans" - is a good small scale way to grow Pans. The method works best if the jars containing the substrate are inoculated with a water suspension of either clean spores or mycelia.




alright nice

is anyone familiar with violet's microwave tek for agar? https://www.shroomery.org/forums/showflat.php/Number/22707554 - microwave agar tek

Since I don't have a PC atm, this seemed very ideal for me. Apparently all he really uses is some BRF which I already have.

Also wanna say I already have 5 jars colonizing ( koh samui strain ) just so everyone knows i'm serious in this thread and am not wasting ya'lls time with these questions. I wanted to start out straight off growing pan cyans but everywhere everyone keeps saying to do the PF tek step by step first.. so I begrudgingly am doing so.

I can't stress enough to people the difference between blue meanies and cubes. Sometime maybe 2 or 3 years ago the cow field near me completely reversed it's normal growth. Usually it'd be 99% cubes and 1% cyans, and the cyans would only show up in the winter. Now, it seems the cyans have completely taken over the entire field strangely enough.

Getting off topic a little but anyway I remember my first trip on pan cyans, I completely underestimated their strength and ate a normal cube dose ( LOL ). Within 15 minutes I got that feeling in my gut that tells you you're about to have an immensely strong trip and bee lined in a dead sprint back to my house ( I like to trip way out back in nature ).

The power of these things are just incredible, if I could describe it to anyone I would say if you take all the good parts of an lsd and mushroom trip and left all the negatives of both out you would have a normal pan cyan trip.

All of their strengths, none of their weaknesses. Amazing lucidity, clearness of mind, great length ( i've still been going STRONG at the 8 hour mark ).. they're just incredible

alright i've poured almost 5-6 hours of study into this and it's getting overwhelming now, i'll be back


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Offlined-pad
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Re: Official Copelandia/Panaeolus Thread [Re: cube talk] * 1
    #25124799 - 04/09/18 02:39 AM (5 years, 9 months ago)

I'm going to leave a comment here so I can follow along. I had some success with Panaeolus Bisporus. Pic in my signature. My last tray of them are in my fruiting chamber right now actually. I have 7 quart jars of Pan Cyan Hawaiian on rye berries that haven't shown any signs of growth since 3/21. My Pan Bisp took 9 days to start colonizing. I don't believe I'll be trying Pan spore syringes again. Apparently oatmeal agar works well.


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Invisiblemary fairchild
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Re: Official Copelandia/Panaeolus Thread [Re: d-pad]
    #25124809 - 04/09/18 02:58 AM (5 years, 9 months ago)

Beautiful Pan grow! Real nice pinset in the first pic. 2nd pic- some dense thickets.
Is the 2nd pic also bisporus?

I look forward to seeing your Pan cy Hawaiian.

Another vote- prints over syringes.


Edited by mary fairchild (04/09/18 03:12 AM)


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OfflineAsuraS
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Re: Official Copelandia/Panaeolus Thread [Re: mary fairchild]
    #25124830 - 04/09/18 03:29 AM (5 years, 9 months ago)

Prints over syringes for sure. Pan syringes always gave me really wispy, slow growth. I have plates
going now (4th transfer) from a print and difference is noticeable. Very strong and aggressive
growth.


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OfflineFailboat
Fuck Up

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Re: Official Copelandia/Panaeolus Thread [Re: Asura] * 1
    #25125441 - 04/09/18 10:39 AM (5 years, 9 months ago)

My syringe gave me some excellent looking thick growths


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InvisibleHumble Newcomer
Diddler de niños
I'm a teapot


Registered: 03/12/17
Posts: 1,483
Re: Official Copelandia/Panaeolus Thread [Re: Failboat] * 1
    #25125657 - 04/09/18 12:15 PM (5 years, 9 months ago)

How could it matter?

As far as germination success, esp on old spores, for sure. I'm proving that with my pan grow big time 5yr old syringe and print both, syringe fired off growth everywhere in every plate. On the print only one or two spots off a whole streak plate, on every streak plate, until I started using an obscene amount of the print for each plate, easily 100x more spores per plate than the single drop of syringe.

But once u have germination you would have equal chance of having good strains. Even if the spores were years old like in my case, I don't see how anything is affected from this point on. They didn't age. That were dormant, lying in wait.

Syringes are made from prints. :shrug:


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How I made my mush GH


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Offlinetryptkaloids
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Re: Official Copelandia/Panaeolus Thread [Re: Humble Newcomer]
    #25125765 - 04/09/18 01:06 PM (5 years, 9 months ago)

It's the extra vector of making the syringe that worries me, even for cubes prints are recamended


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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OfflineDoc9151M
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Re: Official Copelandia/Panaeolus Thread [Re: tryptkaloids]
    #25125976 - 04/09/18 02:33 PM (5 years, 9 months ago)

Quote:

tryptkaloids said:
It's the extra vector of making the syringe that worries me, even for cubes prints are recamended



:whathesaid:


--------------------


Psilocybe cubensis data collection thread. please help with this project if you hunt wild cubensis.
https://www.shroomery.org/forums/showflat.php?Cat=0&Number=26513593&page=0&vc=1#26513593


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InvisibleGerms
Space Force
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Re: Official Copelandia/Panaeolus Thread [Re: Doc9151]
    #25125984 - 04/09/18 02:38 PM (5 years, 9 months ago)

That’s the glory of g2g. 10 jars not good enough but 1 is? Make that 10 more jars. All it takes is one drop
I like inoculating with spore solution, yeah some will go bad but that’s the process


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InvisibleHumble Newcomer
Diddler de niños
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Posts: 1,483
Re: Official Copelandia/Panaeolus Thread [Re: tryptkaloids]
    #25125994 - 04/09/18 02:42 PM (5 years, 9 months ago)

Very true. Way higher chance of having a contammed syringe for that reason. But was that ever a question? That's not what's being voted is it, that's obvious.

On the one hand it is another contamination vector, on the other it makes long term storage much friendlier and much more likely to revive. If u want long long term storage of your spores, a clean syringe in a dark bag in the fridge can last decades.

I read some people have used much older syringes than I'm using and they worked fine, I seem to remember the 20yr number. If done right it's basically the same sterile water storage that the advanced guys use to store their live cultures for 7+ years. Even there, there's a guy who revived his live wedge after 20 years of room temp sterile water storage.

But your getting into lab equip and technique I simply don't have.

At the end of the day, there's no difference in strain creation capability and syringe offers 3x + as long of storage . But who made it, do u have agar, do u have time, well...

So it's completely a case by case basis

Edit : I'm sorry that seems rude in general I think I just misunderstood, if it's a personal preference vote them yeah I guess print all the way prob for everyone as soon as they get into agar.

Though I've been floored with the differences I've been noticing I'm 5yr print vs 5yr syringe in germination, a ten fold difference at five years I basically know that at year 6 or 7 I'll have a zero percent germination rate from print, even applying on really wet agar and leaving two weeks. And I notice no degradation at five years in syringe due yo spore hydration and i read of accounts of people using ten yr plus old syringe. So i thought I'd share.apologies


Edited by Humble Newcomer (04/09/18 03:02 PM)


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OfflineAsuraS
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Re: Official Copelandia/Panaeolus Thread [Re: Humble Newcomer]
    #25126293 - 04/09/18 04:56 PM (5 years, 9 months ago)

In my case, the print was fresh :shrug:


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InvisiblePoison Drink
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Re: Official Copelandia/Panaeolus Thread [Re: Asura]
    #25126415 - 04/09/18 05:42 PM (5 years, 9 months ago)

I'm not having any luck trying to revive 4 year old prints, including Pan. cyanescens. A friend inoculated some agar in a professional flow hood. The available petri dishes contained a high percentage of agar though, making them a bit 'dry'. Since they were inoculated straight from a print, the spores might be having problems with hydrating. After 3 weeks, only one cubensis plate is showing some weak signs of life.

One new Pan. cyanescens spore vial (from a sponsor) was used to inoculate agar. One drop got streaked out over the whole plate, but nothing resembling Pan. mycelium ever germinated. Only contamination grew on a couple of plates, which is weird since the 'control plate' is still contamination free after those three weeks.. Well I should know better ordering spores from a vendor whose main business isn't the sale of spores, but they were based in the EU. Will sent them an email but it's a bit frustrating nonetheless.


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OfflineAsuraS
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Re: Official Copelandia/Panaeolus Thread [Re: Poison Drink]
    #25126429 - 04/09/18 05:45 PM (5 years, 9 months ago)

Sterilize some water and add it to the plate? :shrug:


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InvisibleHumble Newcomer
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Re: Official Copelandia/Panaeolus Thread [Re: Asura]
    #25126446 - 04/09/18 05:51 PM (5 years, 9 months ago)

Your pan cyan "vial", it's a syringe right? You say you put a drop and did a streak plate.

If its not a syringe, consider making a syringe with your old print and force hydrating it for a few days then try again

If it is a syringe, maybe make some divots in a thickly poured plate and put a drop of spore solution in each divot, several divots per dish.

If few spores are germinating we don't want to spread the solution across the whole dish, we want them all close together to more easily meet up and make secondary mycelium


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How I made my mush GH


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Offlinetryptkaloids
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Re: Official Copelandia/Panaeolus Thread [Re: Humble Newcomer]
    #25126504 - 04/09/18 06:14 PM (5 years, 9 months ago)

I bet he means I vial of spore solution. Neither a print or syringe


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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InvisiblePoison Drink
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Re: Official Copelandia/Panaeolus Thread [Re: Humble Newcomer]
    #25126548 - 04/09/18 06:26 PM (5 years, 9 months ago)

The spore vial comes with a sterile syringe and an alcohol swab and was ordered about a month ago. You have to wipe the injection port with an alcohol swab before filling the syringe. No idea why they wouldn't just sent a syringe but it's not much of a hassle anyway. Those plates were streaked since the drop of spore solution wouldn't readily absorb into the agar. Making little indentations into the agar is a great idea, but I'd assume streaking them wouldn't be a problem either with fresh spore solution..?

Plates were also inoculated directly with four years old Pan. cyan spores. They aren't showing any signs of growth after three weeks. I made some homemade no pour agar with a lower percentage of agar, so maybe I should try that print again. How should I go about force hydrating old spores?


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Offlinetryptkaloids
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Re: Official Copelandia/Panaeolus Thread [Re: Poison Drink]
    #25126698 - 04/09/18 07:06 PM (5 years, 9 months ago)

How ridiculous... Force hydration


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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