Home | Community | Message Board

Mycohaus
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Kraken Kratom Red Vein Kratom   Bridgetown Botanicals CBD Concentrates   Mushroom-Hut Liquid Cultures   Original Sensible Seeds Bulk Cannabis Seeds   North Spore Bulk Substrate   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Left Coast Kratom Buy Kratom Extract   PhytoExtractum Maeng Da Thai Kratom Leaf Powder

Jump to first unread post Pages: < First | < Back | 250 | 251 | 252 | 253 | 254 | 255 | 256 | 257 | 258 | 259 | 260 | 261 | 262 | 263 | 264 | 265 | 266 | 267 | 268 | 269 | 270 | Next > | Last >
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
InvisibleGerms
Space Force
 User Gallery

Registered: 06/26/11
Posts: 4,607
Loc: Texas
Trusted Cultivator
Re: Official Copelandia/Panaeolus Thread [Re: Numina]
    #25096460 - 03/28/18 06:53 AM (5 years, 9 months ago)

Quote:

Numina said:
Hi, this is my first post here and I need some advice.  I was lucky enough to have found what I believe to be wild Copelandia cyanescens from which I took a couple of spore prints and managed a single clone on MEA (1 out of 7 petris).  I already had one unsuccessful attempt at growing these using wheat spawn and garden centre cow poo/verm/straw and gypsum.  I’m pretty sure I cased too early and didn’t have enough FAE, maybe the humidity was a bit high too, not sure. 

I am trying again.  I have no trouble generating the spawn using agar and wheat grains ( full colonisation of 1 litre jars in ~10 days).  This time I went out and collected field dried cow poo from the very place I found the mushrooms.  The substrate ratio is roughly cow poo 7 cups, verm 3 cups, coir 2 cups and coffee 1 cup + gypsum at field capacity.  I have also tried straight straw.  Both were pasteurised.  The substrate is in shallow trays less than 2” deep covered by foil with GE holes. Ambient temp is in range 24 to 30 deg C.  My question is there seems to be a lot of moisture on the surface of the mycellium ( in the form of small droplets) as well as a few large drops that have condensed on the foil and dripped down.  Is this normal?




That tub in the second pic looks like it wants to be cased like asap. What are you casing with? And what are we looking at in the last picture?

The condensation is normal, yes


--------------------


Extras: Filter Print Post Top
OfflineSolipsis
m̶a̶d̶ disappointed scientist
Male User Gallery


Registered: 12/28/09
Posts: 3,398
Loc: the Neitherlands Flag
Last seen: 5 months, 19 days
Re: Official Copelandia/Panaeolus Thread [Re: Germs]
    #25096600 - 03/28/18 08:45 AM (5 years, 9 months ago)

@ Mary's plates:

that bottom one doesn't look to me like there is sectoring or different growth types, is just started from a more irregularly shaped wedge? Or is this wrong and do you still get these irregularities when there is a small set of genetics, just with less of a zone of inhibited growth? If so, I'd like to know more about the final steps of isolation.
(I do know about irregularly shaped sectors when the shape of the wedge cannot be blamed, then it is clearly an incomplete isolation.)

I've tried Pan Cyan years ago, went through a whole lot of trouble to cure manure from the pasture (it was really on the fresh side), but my trays did absolutely nothing. Never seemed to contam, just nothing. Maybe yeast?
Had to abandon everything anyway and moved around for a while..

I might be looking at Pan Tropic in the not too distant future. I expect I will be looking into alternatives to poo, substituting with other N sources and ligneous material. I am not put off by poo - I mean it soon doesn't even really smell like that anymore - but I wanna look at materials that may be more easy and consistent. Saw great grows like a couple pages ago with no poo in it.

Experimenting with substituting part of the sub like that seems like an excellent way to unravel what's so 'special' about it. Once you really know more about that the possibilities may be endless.

I should point out that I have successes and experiences in general now and that at one of my jobs I also expect to be experimenting with alternative substrates for edibles. I am not saying at all that I think poo is bad and also my failure with it before was surely because of something I did wrong.
If I can I just wanna avoid overcomplicating things with my home grows and if I can to try not to use manure or straw unless I must.

Could someone take us through sub recipes and explanations for the components? Coffee grounds are rich in proteins and micronutrients and nitrogen and it is lignocelluloid other than that. Straw is also lignocelluloid but can be consumed much better than something like coir I guess? Of course those also help to make the sub more airy. Gypsum for calcium and sulfur. In what sense does adding (extra) coffee grounds not substitute well for manure? Could compost be helpful?


Edited by Solipsis (03/28/18 09:01 AM)


Extras: Filter Print Post Top
InvisibleMycolorado
Hobbyist
Male User Gallery

Registered: 07/23/16
Posts: 8,529
Loc: Interdimensional Bootcamp
Trusted Cultivator
Re: Official Copelandia/Panaeolus Thread [Re: Solipsis] * 1
    #25096606 - 03/28/18 08:50 AM (5 years, 9 months ago)

I'm testing alfalfa supped coir as a poo alternative for Pans.  If it fruits, I’ll start working on varying sup levels to compare.  I’ll have to check my notes but I believe my current mix was 20% sup.  It’s growing slow but looks very strong.


Extras: Filter Print Post Top
OfflineSolipsis
m̶a̶d̶ disappointed scientist
Male User Gallery


Registered: 12/28/09
Posts: 3,398
Loc: the Neitherlands Flag
Last seen: 5 months, 19 days
Re: Official Copelandia/Panaeolus Thread [Re: Mycolorado]
    #25096640 - 03/28/18 09:09 AM (5 years, 9 months ago)

That sounds cool, if it works, a sustainable cheap material besides alfalfa might be duckweed assuming you'd want to grow that? If you can find the right agricultural waste, that may also be an option (though arguably not worth the trouble either for small scales or one time grows)... there should exist some that has nutritional value and there's plenty of them like corn stalks perhaps that are fibrous in nature so it could substitute for straw or coir too?

You think you have enough of a carbon source though?


Extras: Filter Print Post Top
InvisibleMycolorado
Hobbyist
Male User Gallery

Registered: 07/23/16
Posts: 8,529
Loc: Interdimensional Bootcamp
Trusted Cultivator
Re: Official Copelandia/Panaeolus Thread [Re: Solipsis]
    #25096675 - 03/28/18 09:31 AM (5 years, 9 months ago)

Not sure...TBH, I was more concerned with boosting the N.  Here they are, coir poo on left and coir alfalfa on right:



Extras: Filter Print Post Top
InvisibleTheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,941
Re: Official Copelandia/Panaeolus Thread [Re: Mycolorado]
    #25096690 - 03/28/18 09:40 AM (5 years, 9 months ago)

Just throwing this out there. I had one Pan cambo culture that LOVED malt extract. I cased a plate and got small fruits from it. I started a new tropicalis culture and intend on toying with malt extract as a supplementation. If we can find the raw nutrients/ingredients, that the pans feed off of in the poo, we have a good chance. SO what is it in the poo that is probably providing this, WELL I say digested grains. This is why I want to play around with it some. I have no real idea if it will work, but fuck the pan cambo grew faster than some cubes on that agar.


--------------------
JOIN THE POW WOW


Extras: Filter Print Post Top
OfflineWoolyelephant
PanHole
 User Gallery
Registered: 06/18/17
Posts: 71
Last seen: 1 year, 5 months
Re: Official Copelandia/Panaeolus Thread [Re: TheMadHatter420] * 4
    #25097034 - 03/28/18 11:33 AM (5 years, 9 months ago)

First flushers time to pick


Extras: Filter Print Post Top
InvisiblemushboyMDiscord
modboy
 User Gallery


Registered: 04/24/05
Posts: 32,276
Loc: where?
Trusted Cultivator
OG Cultivator
Re: Official Copelandia/Panaeolus Thread [Re: Woolyelephant]
    #25097045 - 03/28/18 11:36 AM (5 years, 9 months ago)

Quote:

Woolyelephant said:
First flushers time to pick





fuck me running. when can i come over, ill pick those for you!!!!


Extras: Filter Print Post Top
InvisibleMycolorado
Hobbyist
Male User Gallery

Registered: 07/23/16
Posts: 8,529
Loc: Interdimensional Bootcamp
Trusted Cultivator
Re: Official Copelandia/Panaeolus Thread [Re: mushboy] * 1
    #25097075 - 03/28/18 11:46 AM (5 years, 9 months ago)

Some nice grows popping up!  Nice work woolyelephant!


Extras: Filter Print Post Top
Invisiblemary fairchild
Pantheist


Registered: 12/06/14
Posts: 777
Trusted Cultivator
Re: Official Copelandia/Panaeolus Thread [Re: Woolyelephant]
    #25097151 - 03/28/18 12:16 PM (5 years, 9 months ago)

Quote:

Woolyelephant said:
First flushers time to pick






They're beautiful!
Looks like some are dropping spores. Those guys mature FAST.


Extras: Filter Print Post Top
OfflinePanarchist
Cyanescendant
 User Gallery


Registered: 04/02/16
Posts: 432
Loc: Copeland
Last seen: 7 months, 17 days
Re: Official Copelandia/Panaeolus Thread [Re: mary fairchild]
    #25097252 - 03/28/18 01:06 PM (5 years, 9 months ago)

nice fruiting, Woolyelephant. keep up the good work.


--------------------


Extras: Filter Print Post Top
OfflineWoolyelephant
PanHole
 User Gallery
Registered: 06/18/17
Posts: 71
Last seen: 1 year, 5 months
Re: Official Copelandia/Panaeolus Thread [Re: Panarchist] * 2
    #25097299 - 03/28/18 01:27 PM (5 years, 9 months ago)

I'll keep you posted.
Here are the stats:
Clone of a clone, agar to LC.
Used rye berries and WBS (not mixed)
Spawned 1:2 ratio
Substrate- 20% steer compost, 30% dried cow poo, 30% coir, 20% vermiculite 5% gypsum Pasteurized.
Consolidated 3 days, 50/50 lime, oyster shell powder to 7.5-8 PH casing sterilized in microwave. Enough to just cover mycelium.
Fruited immediately, GH 95-92% RH
Some fruited in 3 days(WBS) others are starting to pin (7 days).

Any questions feel free to ask.


Extras: Filter Print Post Top
InvisiblemushboyMDiscord
modboy
 User Gallery


Registered: 04/24/05
Posts: 32,276
Loc: where?
Trusted Cultivator
OG Cultivator
Re: Official Copelandia/Panaeolus Thread [Re: Woolyelephant] * 1
    #25097308 - 03/28/18 01:29 PM (5 years, 9 months ago)

isnt steer and cow poop the same thing?


...and 20+30+30+20+5%=105% :aweman:


Extras: Filter Print Post Top
Offlinetryptkaloids
Learner
I'm a teapot


Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 6 hours
Trusted Cultivator
Re: Official Copelandia/Panaeolus Thread [Re: mushboy]
    #25097320 - 03/28/18 01:33 PM (5 years, 9 months ago)

Is that % weight or volume?


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


Extras: Filter Print Post Top
OfflineWoolyelephant
PanHole
 User Gallery
Registered: 06/18/17
Posts: 71
Last seen: 1 year, 5 months
Re: Official Copelandia/Panaeolus Thread [Re: tryptkaloids]
    #25097394 - 03/28/18 02:04 PM (5 years, 9 months ago)

5 % gypsum of total volume not weight.
It's steer manure from Home Depot. I sift out all of the wood and random stuff. Then I also add actual cow poo.
One is composted and the other isn't.



Extras: Filter Print Post Top
OfflineDoc9151M
Mycologist
Male User Gallery


Registered: 02/23/17
Posts: 13,753
Loc: Gulf Coast USA Flag
Last seen: 1 year, 6 months
Trusted Cultivator
Re: Official Copelandia/Panaeolus Thread [Re: bacillus]
    #25097435 - 03/28/18 02:24 PM (5 years, 9 months ago)

To me #1 is a monoculture #2 has obvious sectors and can be isolated further to create a monoculture.

Quote:

bacillus said:
I like mary's use of "monoculture", because as far as I know there's no simple way to tell if something's an isolate from the looks.



An isolate (if its a true isolate) shouldn't have any sectors if its to be a monoculture. Monoculture is one single substrain.


--------------------


Psilocybe cubensis data collection thread. please help with this project if you hunt wild cubensis.
https://www.shroomery.org/forums/showflat.php?Cat=0&Number=26513593&page=0&vc=1#26513593


Extras: Filter Print Post Top
OfflineNumina
Stranger
 User Gallery
Registered: 12/01/17
Posts: 16
Last seen: 3 years, 9 months
Re: Official Copelandia/Panaeolus Thread [Re: Germs]
    #25097622 - 03/28/18 04:04 PM (5 years, 9 months ago)

Hi Germs, Thanks very much for the advice. In my first try I used 50/50+ which I also intend to use for this attempt.  I had the feeling I cased too early the first time and that the substrates weren't fully colonised.  I've read a few posts about letting the substrate consolidate for up to 4 days after full colonisation. As you can tell, I'm not really sure what I'm doing yet. 

The pics below are the same tubs a couple of days later.  The blue tub is the same strain (i think) on straight pasteurised straw.  They seem to be loving it and so far no contam that I can see.  I'll case that tub immediately and see waht happens.  I'll try a lot more FAEs this time and  better lighting.  Not sure about the humidity but I guess I'll try 90-95%. Thanks.




Extras: Filter Print Post Top
OfflineV.L
Male User Gallery

Registered: 12/15/17
Posts: 526
Loc: In my pants
Last seen: 1 hour, 21 minutes
Trusted Cultivator
Re: Official Copelandia/Panaeolus Thread [Re: Woolyelephant]
    #25097707 - 03/28/18 04:46 PM (5 years, 9 months ago)

Really nice grow Wooly!!  I understand why make a clone (better yield and more homogeneous grow...) but what interest to clone a clone? They have exactly same genetic..?


Extras: Filter Print Post Top
Offlinehamloaf
Loaf of Fam.
Male User Gallery

Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma. Flag
Last seen: 1 year, 8 months
Trusted Cultivator
Re: Official Copelandia/Panaeolus Thread [Re: Doc9151]
    #25097791 - 03/28/18 05:15 PM (5 years, 9 months ago)

Quote:

Doc9151 said:
To me #1 is a monoculture #2 has obvious sectors and can be isolated further to create a monoculture.
An isolate (if its a true isolate) shouldn't have any sectors if its to be a monoculture. Monoculture is one single substrain.



No, there can be hundreds of sets genetics on a single plate.  As long as said sets of genetics belong only to one type of mycelial culture than it's a monoculture (single culture).  An isolate is a single set of genetics accomplished by employing 'isolation' technique.  cronicr explains this very well up thread maybe a page or two back.

Quote:

cronicr said:
Monoculture actuslly simply means a single type of growth, in other words a clean dish.
Isolate is an isolated set of genetics.
Over the years it has become habbit to misuse the term monoculture.



https://www.shroomery.org/forums/showflat.php/Number/25095367#25095367

Nice grows, mary, and Woolyelephant.  :thumbup:


--------------------

   
How I Get Stuff done. - My Reference Guide. - My Grows.


Extras: Filter Print Post Top
OfflineAsuraS
Cyantist


Registered: 08/01/11
Posts: 5,047
Loc: Texas
Last seen: 11 days, 19 hours
Trusted Cultivator
Re: Official Copelandia/Panaeolus Thread [Re: Woolyelephant]
    #25097796 - 03/28/18 05:18 PM (5 years, 9 months ago)

Quote:

Woolyelephant said:
First flushers time to pick





This just blows my mind.


Extras: Filter Print Post Top
Jump to top Pages: < First | < Back | 250 | 251 | 252 | 253 | 254 | 255 | 256 | 257 | 258 | 259 | 260 | 261 | 262 | 263 | 264 | 265 | 266 | 267 | 268 | 269 | 270 | Next > | Last >

Shop: Kraken Kratom Red Vein Kratom   Bridgetown Botanicals CBD Concentrates   Mushroom-Hut Liquid Cultures   Original Sensible Seeds Bulk Cannabis Seeds   North Spore Bulk Substrate   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Left Coast Kratom Buy Kratom Extract   PhytoExtractum Maeng Da Thai Kratom Leaf Powder


Similar ThreadsPosterViewsRepliesLast post
* The official Psilocybe natalensis thread
( 1 2 3 4 ... 515 516 )
Hillbillyjohn 328,253 10,314 01/29/24 04:30 PM
by NotFromGeorgia
* Official Misc EXOTIC Thread
( 1 2 3 4 ... 132 133 )
Jakeoncid419 110,110 2,651 01/29/24 04:42 PM
by EniQma
* Rye-Z-Morph Bag for Panaeolus (Copelandia) Cyanescens? rob_531 4,313 12 01/10/05 12:33 PM
by agar
* Copelandia "Pan" Cyanescens sancho 4,962 6 09/07/07 10:00 AM
by suave
* Re: look at these copelandia cyanescens they are beautiful Anonymous 5,161 2 12/25/99 07:16 AM
by sean123
* which tek for copelandia cyanescens (spore print)? FfF 7,484 10 08/26/04 05:44 AM
by hawksapprentice
* Hawaiian Copelandia Grow Kit - how much more difficult are they? GabrielKnight 8,125 5 01/03/05 04:59 PM
by ticktock
* Preparing jars for Copelandia? PawPaw 1,195 14 04/02/05 01:11 PM
by PawPaw

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
924,121 topic views. 13 members, 204 guests and 45 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.031 seconds spending 0.012 seconds on 13 queries.