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OfflinecronicrFacebook
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Re: Official Copelandia/Panaeolus Thread [Re: mary fairchild] * 1
    #25095367 - 03/27/18 05:31 PM (5 years, 9 months ago)

Monoculture actuslly simply means a single type of growth, in other words a clean dish.
Isolate is an isolated set of genetics.
Over the years it has become habbit to misuse the term monoculture.


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It doesn't matter what i think of you...all that matters is clean spawn

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Invisiblemary fairchild
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Re: Official Copelandia/Panaeolus Thread [Re: cronicr] * 9
    #25095537 - 03/27/18 06:36 PM (5 years, 9 months ago)

Here’s  2nd flush of Pan. bisporus:


The first flush lingered for awhile- so I just harvested as much as I could then soaked the jars in water for 15 minutes.

I really think it helped to get a nice second growth.

Heres a closeup:


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InvisibleMycolorado
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Re: Official Copelandia/Panaeolus Thread [Re: mary fairchild]
    #25095632 - 03/27/18 07:03 PM (5 years, 9 months ago)

Nice; what a killer grow, mary fairchild!  That first round I ran did the same thing...first fruit were smaller and kinda funny shaped followed by much nicer looking and larger fruit.  Anyway, those are beautiful!


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Invisiblemary fairchild
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Re: Official Copelandia/Panaeolus Thread [Re: Mycolorado]
    #25095657 - 03/27/18 07:13 PM (5 years, 9 months ago)

I don't know if i'll get a third round- I'll harvest most of these tomorrow- if they are like the first batch, they will keep producing for awhile.

The top pic- lower right hand corner- some of those guys are dark brown, guess its just a new trait appearing.


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InvisibleMycolorado
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Re: Official Copelandia/Panaeolus Thread [Re: mary fairchild]
    #25095711 - 03/27/18 07:43 PM (5 years, 9 months ago)

Yeah, mine did the same thing after the nice second flush...small and rusty brown.  I thought it was just dry but maybe that’s how they do. 



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Invisiblemary fairchild
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Re: Official Copelandia/Panaeolus Thread [Re: Mycolorado]
    #25095733 - 03/27/18 07:59 PM (5 years, 9 months ago)

Are those bis?

Some of them seem to have really thick stems- regardless they look great!


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InvisibleMycolorado
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Re: Official Copelandia/Panaeolus Thread [Re: mary fairchild]
    #25095740 - 03/27/18 08:06 PM (5 years, 9 months ago)

Yep.  Ha, they were little nubs but definitely had a cool cap color.


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Offlinetryptkaloids
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Re: Official Copelandia/Panaeolus Thread [Re: cronicr]
    #25095757 - 03/27/18 08:15 PM (5 years, 9 months ago)

Quote:

cronicr said:
Monoculture actuslly simply means a single type of growth, in other words a clean dish.
Isolate is an isolated set of genetics.
Over the years it has become habbit to misuse the term monoculture.



I wonder why it's been misconstrued. I think it isn't used enough


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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Invisibleshroomerite
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Re: Official Copelandia/Panaeolus Thread [Re: cronicr]
    #25095770 - 03/27/18 08:24 PM (5 years, 9 months ago)


Righto, monoculture actually means the growth of a single organism, which can mean just a mushroom (which can have multiple genetics). Isolate is the correct term you are looking for, to refer to when referencing a single strain (genetic) of growth of that monoculture.


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OfflineDoc9151M
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Re: Official Copelandia/Panaeolus Thread [Re: shroomerite]
    #25096082 - 03/27/18 11:28 PM (5 years, 9 months ago)

Monoculture has zero sectors, it's a pure culture. I should probably say its 1 phenotype.


--------------------


Psilocybe cubensis data collection thread. please help with this project if you hunt wild cubensis.
https://www.shroomery.org/forums/showflat.php?Cat=0&Number=26513593&page=0&vc=1#26513593


Edited by Doc9151 (03/27/18 11:29 PM)


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OfflineNumina
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Re: Official Copelandia/Panaeolus Thread [Re: shroomerite]
    #25096151 - 03/28/18 12:22 AM (5 years, 9 months ago)

Hi, this is my first post here and I need some advice.  I was lucky enough to have found what I believe to be wild Copelandia cyanescens from which I took a couple of spore prints and managed a single clone on MEA (1 out of 7 petris).  I already had one unsuccessful attempt at growing these using wheat spawn and garden centre cow poo/verm/straw and gypsum.  I’m pretty sure I cased too early and didn’t have enough FAE, maybe the humidity was a bit high too, not sure. 

I am trying again.  I have no trouble generating the spawn using agar and wheat grains ( full colonisation of 1 litre jars in ~10 days).  This time I went out and collected field dried cow poo from the very place I found the mushrooms.  The substrate ratio is roughly cow poo 7 cups, verm 3 cups, coir 2 cups and coffee 1 cup + gypsum at field capacity.  I have also tried straight straw.  Both were pasteurised.  The substrate is in shallow trays less than 2” deep covered by foil with GE holes. Ambient temp is in range 24 to 30 deg C.  My question is there seems to be a lot of moisture on the surface of the mycellium ( in the form of small droplets) as well as a few large drops that have condensed on the foil and dripped down.  Is this normal?


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Invisibleshroomerite
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Re: Official Copelandia/Panaeolus Thread [Re: Numina]
    #25096161 - 03/28/18 12:30 AM (5 years, 9 months ago)

Quote:

Numina said:My question is there seems to be a lot of moisture on the surface of the mycellium ( in the form of small droplets) as well as a few large drops that have condensed on the foil and dripped down.  Is this normal?




Completely normal


--------------------
  "For best results, learn to work with nature rather than against it. Mycelium has an amazing ability to cope with less than optimal conditions, and will often fruit when a grower does everything wrong. However, do everything right and watch your performance go through the roof." RR



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Invisiblemary fairchild
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Re: Official Copelandia/Panaeolus Thread [Re: Doc9151] * 2
    #25096176 - 03/28/18 12:48 AM (5 years, 9 months ago)

Quote:

Doc9151 said:
Monoculture has zero sectors, it's a pure culture. I should probably say its 1 phenotype.




I'm surprised- this has been going on for awhile:laugh:
I hope we are all on the same page with this.

I've been calling this a monoculture:


Just because its a single growth form as opposed to:


To distinguish one from the other- its easier to say "monoculture" than "symmetric growth form" or some other derivative. I'm using the word in its simplest form without reading in to it too much.
I'm open to a better word for it.


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Invisibleshroomerite
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Re: Official Copelandia/Panaeolus Thread [Re: mary fairchild]
    #25096183 - 03/28/18 12:59 AM (5 years, 9 months ago)

Monoculture means the growth of a single organism which means a mushroom. Has nothing to do with sectors.


--------------------
  "For best results, learn to work with nature rather than against it. Mycelium has an amazing ability to cope with less than optimal conditions, and will often fruit when a grower does everything wrong. However, do everything right and watch your performance go through the roof." RR



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OfflinecronicrFacebook
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Re: Official Copelandia/Panaeolus Thread [Re: mary fairchild]
    #25096187 - 03/28/18 01:03 AM (5 years, 9 months ago)

Quote:

mary fairchild said:
Quote:

Doc9151 said:
Monoculture has zero sectors, it's a pure culture. I should probably say its 1 phenotype.




I'm surprised- this has been going on for awhile:D
I hope we are all on the same page with this.

I've been calling this a monoculture:


Just because its a single growth form as opposed to:


To distinguish one from the other- its easier to say "monoculture" than "symmetric growth form" or some other derivative. I'm using the word in its simplest form without reading in to it too much.
I'm open to a better word for it.



Isolate


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Invisiblemary fairchild
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Re: Official Copelandia/Panaeolus Thread [Re: cronicr]
    #25096204 - 03/28/18 01:13 AM (5 years, 9 months ago)

Really,
It only took 2 passages from the plate that was germinated with spores "P2 on the top". I know that Pans differ from cubes but... Isn't that impossible with cubes, and if so- hmm.


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OfflinecronicrFacebook
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Re: Official Copelandia/Panaeolus Thread [Re: mary fairchild]
    #25096212 - 03/28/18 01:20 AM (5 years, 9 months ago)

Lol i was being cheeky but any species from spires normally start that way because you are seeing strains on top of strains beside strains lol, often sectoring wont happen for at least a few transfers so keeping shit simple its called a ms culture and the end game being an isolate.


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Invisiblemary fairchild
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Re: Official Copelandia/Panaeolus Thread [Re: cronicr]
    #25096221 - 03/28/18 01:32 AM (5 years, 9 months ago)

Oh well,
I was getting ready to frame it on my wall.:laugh:


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OfflineNumina
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Re: Official Copelandia/Panaeolus Thread [Re: shroomerite]
    #25096296 - 03/28/18 02:58 AM (5 years, 9 months ago)

Thanks for that. Is there any need to mop up any excess moisture from the top of the myc with a paper towel?


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Invisiblebacillus
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Re: Official Copelandia/Panaeolus Thread [Re: cronicr]
    #25096310 - 03/28/18 03:24 AM (5 years, 9 months ago)

I like mary's use of "monoculture", because as far as I know there's no simple way to tell if something's an isolate from the looks.


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Mycelium on agarLet's trade


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