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Primal Call
Earth Mage



Registered: 09/05/10
Posts: 2,766
Loc: Here until here
Last seen: 4 years, 3 months
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Quote:
TheMadHatter420 said: I say check it with a nickel as those should be 4.9-5.1g. Then you can get more accurate as 500ml would uses the equivalent of 2 nickels' weight.
Any US bill should be 1g if that helps
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Xclozure
Stoned as shiiitakes



Registered: 09/09/12
Posts: 421
Last seen: 1 year, 6 months
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Re: Official Copelandia/Panaeolus Thread [Re: Primal Call]
#24758557 - 11/04/17 07:39 AM (6 years, 2 months ago) |
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Are we shaking pan cyan jars now a days
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D3v1ll
Enlightened



Registered: 09/29/17
Posts: 115
Last seen: 4 years, 10 months
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Re: Official Copelandia/Panaeolus Thread [Re: Xclozure]
#24758570 - 11/04/17 07:48 AM (6 years, 2 months ago) |
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I’ve had mixed results personally
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Xclozure
Stoned as shiiitakes



Registered: 09/09/12
Posts: 421
Last seen: 1 year, 6 months
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Re: Official Copelandia/Panaeolus Thread [Re: D3v1ll]
#24758630 - 11/04/17 08:24 AM (6 years, 2 months ago) |
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Yea I shook my multi spores an they didn't bounce back, but just curious wat people think about shaking agar? If it's clean will it cover the whole thing or is there still a chance it could unevenly colonize Idc about the speed just don't want it to be uncolonized
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Snazz
Polymath



Registered: 11/24/15
Posts: 1,584
Loc: Canada
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Re: Official Copelandia/Panaeolus Thread [Re: Xclozure]
#24758641 - 11/04/17 08:31 AM (6 years, 2 months ago) |
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Using this LC once my 11L mycobag of bulk sub sterilizes and cools this AM:

Couple chunks are from the transfer media
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Germs
Space Force


Registered: 06/26/11
Posts: 4,607
Loc: Texas
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Re: Official Copelandia/Panaeolus Thread [Re: Snazz]
#24758725 - 11/04/17 09:18 AM (6 years, 2 months ago) |
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Every Panaeolus jar I have shaken has recovered fine... no idea where that info cones from
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Snazz
Polymath



Registered: 11/24/15
Posts: 1,584
Loc: Canada
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Re: Official Copelandia/Panaeolus Thread [Re: Xclozure]
#24758742 - 11/04/17 09:24 AM (6 years, 2 months ago) |
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Quote:
Xclozure said: Yea I shook my multi spores an they didn't bounce back, but just curious wat people think about shaking agar? If it's clean will it cover the whole thing or is there still a chance it could unevenly colonize Idc about the speed just don't want it to be uncolonized
My LC has been swirled daily. Stir plate and bar is what a proper setup would use for faster growth. You're not really worried about reconnections at this stage.
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Xclozure
Stoned as shiiitakes



Registered: 09/09/12
Posts: 421
Last seen: 1 year, 6 months
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Re: Official Copelandia/Panaeolus Thread [Re: Snazz]
#24758783 - 11/04/17 09:39 AM (6 years, 2 months ago) |
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Quote:
Snazz said:
Quote:
Xclozure said: Yea I shook my multi spores an they didn't bounce back, but just curious wat people think about shaking agar? If it's clean will it cover the whole thing or is there still a chance it could unevenly colonize Idc about the speed just don't want it to be uncolonized
My LC has been swirled daily. Stir plate and bar is what a proper setup would use for faster growth. You're not really worried about reconnections at this stage.
wat do u mean snazz
Edited by Xclozure (11/04/17 10:03 AM)
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Snazz
Polymath



Registered: 11/24/15
Posts: 1,584
Loc: Canada
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Re: Official Copelandia/Panaeolus Thread [Re: Xclozure]
#24758869 - 11/04/17 10:12 AM (6 years, 2 months ago) |
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Nm, I thought your ldc was about shaking agar and lc lol.
I haven't shaken my poo-blend jars as it's fairly firm. Although I did xfer some top growth to agar to clean up and expand to LC
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Universe Unknown
Copes Enthusiast


Registered: 04/29/17
Posts: 86
Last seen: 5 years, 11 days
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Quote:
TheMadHatter420 said: Well I usually make up 500ml at a time and use between 8 and 10g LME depending on how I am feeling. If I know I have contamination I will go light to encourage it to grow across the plate faster hunting food. Stronger mixes will grow slower. When I first started agar I got a pre mix and had to use 25g per 500ml, so that would have had to been 15g LME and 10g agar. I found stuff grew slow as it didn't have to hunt for nutrition. So I started mixing my own, cut back the nutrition and stuff grew quicker.
That's pretty informative, I never knew nutrition level could have an impact on myc spreading out. Is it possible to manage this effect with a premade mix? My premade mix is supposed to be used at a 1:20 ratio with water. If I do 1:25 or 1:30 would that work to reduce nutrition or would it just fuck up my agar mix since I would be reducing both agar and nutrition instead of just nutrition?
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Universe Unknown
Copes Enthusiast


Registered: 04/29/17
Posts: 86
Last seen: 5 years, 11 days
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Does this oat jar look fucked to you guys? I have 7 jars that are two weeks old with fairly decent growth but there seems to be patches of green/blue/grayish in there that are really worrying me. The discoloration only started to show up in the last few days

On the bright side, the Macmerdin Style pan-cakes that I inoculated last week seem to be aggressively colonizing:
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DarkStar2000
Stranger

Registered: 11/05/17
Posts: 11
Last seen: 2 years, 5 months
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Thank you for this, it is very helpful!
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D3v1ll
Enlightened



Registered: 09/29/17
Posts: 115
Last seen: 4 years, 10 months
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Re: Official Copelandia/Panaeolus Thread [Re: DarkStar2000]
#24761129 - 11/05/17 10:00 AM (6 years, 2 months ago) |
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I’m really liking mcmerdins style pan-cakes
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Universe Unknown
Copes Enthusiast


Registered: 04/29/17
Posts: 86
Last seen: 5 years, 11 days
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Re: Official Copelandia/Panaeolus Thread [Re: D3v1ll]
#24761405 - 11/05/17 12:00 PM (6 years, 2 months ago) |
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Quote:
D3v1ll said: I’m really liking mcmerdins style pan-cakes

Nice containers you have there. What type/brand are they if you don't mind my asking? They seem a bit bigger than the ziplocs that MacMerdin put in his writeup.
Also what yield do you typically get from pan-cakes?
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D3v1ll
Enlightened



Registered: 09/29/17
Posts: 115
Last seen: 4 years, 10 months
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They are 9 oz. I couldn’t find the 8 oz ones so I just made a tiny bit more following the tek. I believe they are glad. I got them from Walmart. You can expect to avg 2g dry from each of u follow the tek perfectly
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D3v1ll
Enlightened



Registered: 09/29/17
Posts: 115
Last seen: 4 years, 10 months
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Re: Official Copelandia/Panaeolus Thread [Re: D3v1ll]
#24761614 - 11/05/17 01:04 PM (6 years, 2 months ago) |
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Also universe... what pans are you growing in that jar with the discoloration? Could it be psuedosclerotia?
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Universe Unknown
Copes Enthusiast


Registered: 04/29/17
Posts: 86
Last seen: 5 years, 11 days
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Re: Official Copelandia/Panaeolus Thread [Re: D3v1ll]
#24761652 - 11/05/17 01:18 PM (6 years, 2 months ago) |
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Thanks for the info. Those are Pan Cyans. It came from a wild print that I printed myself. Do Pans Cyans typically do that?
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D3v1ll
Enlightened



Registered: 09/29/17
Posts: 115
Last seen: 4 years, 10 months
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I hear that they can. I’m sure someone will chime in who knows better. You can also go to the tc thread and ask
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Mycolorado
Hobbyist


Registered: 07/23/16
Posts: 8,529
Loc: Interdimensional Bootcamp
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Re: Official Copelandia/Panaeolus Thread [Re: D3v1ll]
#24762227 - 11/05/17 05:41 PM (6 years, 2 months ago) |
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The Blob:
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Official Copelandia/Panaeolus Thread [Re: Mycolorado]
#24762255 - 11/05/17 05:50 PM (6 years, 2 months ago) |
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Yes pan cyans can form a psuedo stone like growth it is not typical but it happens. Shaking pans is still one of those mixed results type thing lots have no issues
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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