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OfflinePsilosopherr
A psilly goose
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Registered: 02/15/12
Posts: 12,278
Last seen: 1 month, 10 days
Re: Official Copelandia/Panaeolus Thread [Re: Mad Season]
    #22392807 - 10/17/15 11:00 AM (8 years, 3 months ago)

very good to know madseason :cool2:

This thread has been quite informative


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OfflineICEMANOO9
Ssi


Registered: 06/25/12
Posts: 209
Last seen: 1 year, 6 days
Re: Official Copelandia/Panaeolus Thread [Re: Psilosopherr]
    #22392896 - 10/17/15 11:23 AM (8 years, 3 months ago)

This is some deemsters I extracted
And this pan cyan looks like its just about to knot


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OfflinePsilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
Re: Official Copelandia/Panaeolus Thread [Re: ICEMANOO9]
    #22392914 - 10/17/15 11:28 AM (8 years, 3 months ago)

:hellyeah:  You gonna clean up that spice a bit?


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OfflineICEMANOO9
Ssi


Registered: 06/25/12
Posts: 209
Last seen: 1 year, 6 days
Re: Official Copelandia/Panaeolus Thread [Re: Psilosoulful]
    #22393205 - 10/17/15 01:06 PM (8 years, 3 months ago)

I intend to extract the rest of my 300 grams this was 100. Than recrystal the whole bunch and try to produce a huge stalagtite.


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OfflinePsilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
Re: Official Copelandia/Panaeolus Thread [Re: ICEMANOO9]
    #22393284 - 10/17/15 01:29 PM (8 years, 3 months ago)

:headbanger:


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InvisibleFantastic Mr. Fox
Grass Seed Connoisseur


Registered: 02/12/15
Posts: 3,225
Loc: Flag
Re: Official Copelandia/Panaeolus Thread [Re: ICEMANOO9]
    #22393358 - 10/17/15 01:49 PM (8 years, 3 months ago)

Nice,
That Jungle spice need a heptane re-X asap, if you want tht Elfspice  lol

I'll be doing an extraction as well sometime this month or next.


Anyway back on topic,

Yall should state/decipher "cyan/cyanescens", "cam/cambodginesis", "subb/subbalteatus/cinctulus"...etc
I hear see pan and I get all confused as to which one lol
cyans are stronger than cinctulus, but weaker than cambodginesis


--------------------
Giving is all we have, for we're just grateful to be alive

:kratom:


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InvisibleFantastic Mr. Fox
Grass Seed Connoisseur


Registered: 02/12/15
Posts: 3,225
Loc: Flag
Re: Official Copelandia/Panaeolus Thread [Re: Fantastic Mr. Fox]
    #22394944 - 10/17/15 07:33 PM (8 years, 3 months ago)

Copelandia cyanescens (2nd flush)



I'm really loving RGS,


--------------------
Giving is all we have, for we're just grateful to be alive

:kratom:


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OfflineICEMANOO9
Ssi


Registered: 06/25/12
Posts: 209
Last seen: 1 year, 6 days
Re: Official Copelandia/Panaeolus Thread [Re: Fantastic Mr. Fox]
    #22395734 - 10/17/15 10:33 PM (8 years, 3 months ago)

Hey Mr fox no intent to thread jack here's a neat idea for schiphoning that pesky naptha layer off

If you have an extra syringe , needle  and some tubing that fits. Works like a dream


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InvisibleFantastic Mr. Fox
Grass Seed Connoisseur


Registered: 02/12/15
Posts: 3,225
Loc: Flag
Re: Official Copelandia/Panaeolus Thread [Re: ICEMANOO9] * 1
    #22395765 - 10/17/15 10:45 PM (8 years, 3 months ago)

You should never let your solvents touch plastic...

Buy a pipette, and stop being cheap...Or your risking your health.
If thats whats your into, then by all means "ghetto rigg" away..


--------------------
Giving is all we have, for we're just grateful to be alive

:kratom:


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OfflineICEMANOO9
Ssi


Registered: 06/25/12
Posts: 209
Last seen: 1 year, 6 days
Re: Official Copelandia/Panaeolus Thread [Re: Fantastic Mr. Fox]
    #22395789 - 10/17/15 10:55 PM (8 years, 3 months ago)

Yeah your right about that. Now that I look at it the plastic is stained yellow. Yuck


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OfflinefirstTIMER420
Born the son of a sharecropper..
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Registered: 05/08/15
Posts: 1,025
Loc: US, maybe?
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Re: Official Copelandia/Panaeolus Thread [Re: Fantastic Mr. Fox]
    #22395864 - 10/17/15 11:18 PM (8 years, 3 months ago)

Quote:

Fantastic Mr. Fox said:
Quote:

firstTIMER420 said:
Can someone post some recent pics of an agar plate after MS inoculation of Pan cyan spores, and a plate after rthe first or second transfer?  I cant seem the culture to "thrive"  it stays flat on the plate and doesn't really look like much, but its there.  really starting to get annoying and Ive switched mediums so im not sure if its the agar blend.  Ive also tried switching strain and source as well.




Here ya go..


If you really want help you should post a picture of your dish..
No two cultures are the same... They're like snowflakes





All my plates look lke this
shits fucked up dude, I CANNOT get a good strain to germinate, idk if its the agar formulation or what.  But, ive been mixing my own agar lately and its been working fine for every other strain.  The myc is barely visible, I cann see it climbing on the plate, but it isn't vertical at all, flat.

I have some pan cyan germinating on Rye grain straight from the ms syringe and im going to try grabbing a couple pieces of rye and plopping them on a plate :shrug:


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12 34 http://www.shroomery.org/forums/showflat.php/Number/22386794[/url]
--Anything posted by this account is completely fictitious--


Edited by firstTIMER420 (10/17/15 11:20 PM)


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OfflineICEMANOO9
Ssi


Registered: 06/25/12
Posts: 209
Last seen: 1 year, 6 days
Re: Official Copelandia/Panaeolus Thread [Re: ICEMANOO9]
    #22395875 - 10/17/15 11:21 PM (8 years, 3 months ago)

I'm curious how do you schiphon off the solvent layer?


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InvisibleFantastic Mr. Fox
Grass Seed Connoisseur


Registered: 02/12/15
Posts: 3,225
Loc: Flag
Re: Official Copelandia/Panaeolus Thread [Re: firstTIMER420]
    #22395884 - 10/17/15 11:23 PM (8 years, 3 months ago)

That's not pan mycelia.
More than likely mold.

What's you agar media recipe?
What are you inoculating the agar media with? Syringe?

Edit: G2A could work out better for you.


--------------------
Giving is all we have, for we're just grateful to be alive

:kratom:


Edited by Fantastic Mr. Fox (10/17/15 11:38 PM)


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InvisibleFantastic Mr. Fox
Grass Seed Connoisseur


Registered: 02/12/15
Posts: 3,225
Loc: Flag
Re: Official Copelandia/Panaeolus Thread [Re: ICEMANOO9]
    #22395890 - 10/17/15 11:25 PM (8 years, 3 months ago)

Quote:

ICEMANOO9 said:
I'm curious how do you schiphon off the solvent layer?




I use a pipette.
If I'm lazy, I bust out the glass turkey baster.
(Never letting the solvent touch the rubber plunger)


--------------------
Giving is all we have, for we're just grateful to be alive

:kratom:


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Offlineaerow.thefox
professional amatuer
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Registered: 06/10/10
Posts: 316
Loc: living w/dimensional elve...
Last seen: 3 years, 1 day
Re: Official Copelandia/Panaeolus Thread [Re: Fantastic Mr. Fox]
    #22395925 - 10/17/15 11:39 PM (8 years, 3 months ago)



plate T2. this is from a couple days ago right when i was taking my transfer.



heres T3. lookin good to you guys? sorry its so fuzzy. damn camera doesnt wanna focus IN the glass.


--------------------
need a pc build list? i got you



Edited by aerow.thefox (10/17/15 11:41 PM)


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Invisiblejbaby007
Badass
I'm a teapot


Registered: 02/28/15
Posts: 1,026
Re: Official Copelandia/Panaeolus Thread [Re: aerow.thefox]
    #22395979 - 10/18/15 12:00 AM (8 years, 3 months ago)

I have my pan cyan spores on agar now. Been following this thread and hope to get it right the first time  :justcantwait:


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OfflinePsilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
Re: Official Copelandia/Panaeolus Thread [Re: jbaby007]
    #22396447 - 10/18/15 06:54 AM (8 years, 3 months ago)

Me too! :toast:

I knocked up some plates two days ago. I placed a drop of MS solution on a sterilized q-tip and swiped the agar in the middle of the plate and the other I just made a couple of small indentations in the agar and put a single drop in the middle.

I wanna see which method has a faster growth rate.

I'm also using glass jars for my agar. There was no condensation on the sides of the jar after the PC cycle, so I was able to use them right away. Last time I made pasty plates, it took like 3 days for all the condensation to clear up.


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Invisiblejanica
Female


Registered: 05/05/15
Posts: 57
Re: Official Copelandia/Panaeolus Thread [Re: Fantastic Mr. Fox]
    #22396800 - 10/18/15 09:25 AM (8 years, 3 months ago)

Quote:

Fantastic Mr. Fox said:
Copelandia cyanescens (2nd flush)



I'm really loving RGS,




Great shots! amazing grow as allways :sun:


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OfflinefirstTIMER420
Born the son of a sharecropper..
Male User Gallery


Registered: 05/08/15
Posts: 1,025
Loc: US, maybe?
Last seen: 6 years, 4 days
Re: Official Copelandia/Panaeolus Thread [Re: Fantastic Mr. Fox]
    #22396933 - 10/18/15 10:12 AM (8 years, 3 months ago)

Quote:

Fantastic Mr. Fox said:
That's not pan mycelia.
More than likely mold.

What's you agar media recipe?
What are you inoculating the agar media with? Syringe?

Edit: G2A could work out better for you.




Using a Syringe for inoculation.  using MEA currently but have tried PDA as well.  Ive used 4 different syringes and 1 had str8 bacteria on the plates, and the next two times I got this.  Different strins and everything.  Ive even used prints, but the prints didn't germinate at all.

whats your process for G2A, do you shake or not shake the jar to loosten them up.  Also, ive been thinking about injecting sterile water, then shaking and getting back the water and using that for inoculation of the plates, but there seems to be metabolites in some of the jars.


--------------------
12 34 http://www.shroomery.org/forums/showflat.php/Number/22386794[/url]
--Anything posted by this account is completely fictitious--


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OfflineICEMANOO9
Ssi


Registered: 06/25/12
Posts: 209
Last seen: 1 year, 6 days
Re: Official Copelandia/Panaeolus Thread [Re: firstTIMER420] * 1
    #22396950 - 10/18/15 10:17 AM (8 years, 3 months ago)

Try adding a little horse poo to your agar and see if that helps couldn't hurt to try


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