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InvisibleMateja
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Re: Official Copelandia/Panaeolus Thread [Re: Xclozure]
    #24691973 - 10/07/17 06:55 PM (6 years, 3 months ago)

The dark spots are developing fruit bodies, they can be transferred as clones.


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OfflineXclozure
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Re: Official Copelandia/Panaeolus Thread [Re: Mateja]
    #24692010 - 10/07/17 07:03 PM (6 years, 3 months ago)

Relleee. hmmm. thank you.


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OfflineXclozure
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Re: Official Copelandia/Panaeolus Thread [Re: Xclozure]
    #24692039 - 10/07/17 07:11 PM (6 years, 3 months ago)

definately weird though because noobs will confuse that with contam XD


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InvisibleMateja
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Re: Official Copelandia/Panaeolus Thread [Re: Xclozure]
    #24692211 - 10/07/17 08:03 PM (6 years, 3 months ago)

This is the plate that germinated (it's the same plate in all three pics)




And this is the first transfer from that plate: (This pic is really bad I know)




Some have told me it could be mold, others have said it's Pan myc, I honestly don't know if I am transferring mold or Pan Cyan. I'd rather stop transferring if it's mold :smile:


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Offlinetryptkaloids
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Re: Official Copelandia/Panaeolus Thread [Re: Mateja]
    #24692214 - 10/07/17 08:04 PM (6 years, 3 months ago)

that's the biggest struggle I have had and seen others to have. is it mold or mycelium? hard to tell with pans IME. I have yet to have anything fruit


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"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
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OfflinePanarchist
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Re: Official Copelandia/Panaeolus Thread [Re: tryptkaloids]
    #24692290 - 10/07/17 08:25 PM (6 years, 3 months ago)

Mateah, that first transfer is looking good. i would suggest you transfer the leading edge from that zone around the 3:00 position as soon as possible. and don't let any of your follow up plates get overgrown. hit them up once the good growth reveals itself, usually around 4 days.

tryptkaloids, have you tried any colonized grain transfers? since the mycelium is already active the growth spreads out alot quicker. then if you just take those leading edges you're good to go.


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Offlinetryptkaloids
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Re: Official Copelandia/Panaeolus Thread [Re: Panarchist]
    #24692317 - 10/07/17 08:31 PM (6 years, 3 months ago)

I haven't touched pans in awhile. I don't feel comfortable trying again until I am more confident with cubes. I need to either change my water source or change my grain prep I think because I can't seem to stay away from bacteria


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"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
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Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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InvisibleSnazz
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Re: Official Copelandia/Panaeolus Thread [Re: tryptkaloids]
    #24696668 - 10/09/17 12:57 PM (6 years, 3 months ago)

Good day Shoomerites,

I have 3 varieties of Copelandia coming in, large amount of field aged hpoo, mycobags and all my existing supplies from cube/mexicana projects.

Haven't worked with this bulk sub before. Any tips on prepping hpoo? Tools or techniques for breaking up the masses. Pasteurization vs PC and blending in RGS. Verm and coir seem to be the other 2 main ingredients.

If I use hull on oats, I likely won't have to worry about RGS, as that nutritive source lasts quite some time.

If I have 4L bulk sub per, and want 2" depth, do I just open, crumble and level to a shallow sterilite tub. Or should it be bulked out again @ spawning?

Will likely play around with some additives like stevia leaf, canola oil and alfalfa.

As for cultures, seems agar to LI is spoken of well. Second LC and wedges for this species.

They are P. Cambo Thai, Copelandia Hawaiian and Jamaica, and Tropicalis

Thanks for any advice. Will start up a growlog when I'm actively working on it.

:toast:


Edited by Snazz (10/09/17 03:09 PM)


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OfflineXclozure
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Re: Official Copelandia/Panaeolus Thread [Re: Snazz] * 1
    #24699232 - 10/10/17 10:11 AM (6 years, 3 months ago)

pan cyan jam...what are these thooo.


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OfflinePanarchist
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Re: Official Copelandia/Panaeolus Thread [Re: Xclozure] * 1
    #24699604 - 10/10/17 02:00 PM (6 years, 3 months ago)

Xclozure, the only thing i see that looks like it might be concerning are those bumpy nodules on the mycelium in the lower area. with the way the light is hitting it though it's hard to say if that's infection, mycelium or pins.

Snazz, good luck with your project. i don't have any tips for using hpoo but there a few teks on the forum. and if your growth medium includes manure you can crumble and case that to tray/tubs but if it's just grains you'll do best to spawn that to a manure substrate. and i haven't seen any evidence that the additives you mentioned are any benefit to pan mycelium but it never hurts to experiment. i would suggest you get a few successful grows under your belt before you include any additives so that you can establish a baseline of what your results are not including them. and i've seen a few pan growers state that blended liquid inoculate can be problematic because pan mycelium is so fragile it gets damaged from it but i'm not sure if that represents a consensus viewpoint. either way, LC or wedges will work fine.


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OfflineXclozure
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Re: Official Copelandia/Panaeolus Thread [Re: Panarchist]
    #24699630 - 10/10/17 02:11 PM (6 years, 3 months ago)

Yeah im quite unsure it was really rhizo last week but its grew since then =p ill stick it out doe


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Offlinetryptkaloids
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Re: Official Copelandia/Panaeolus Thread [Re: Xclozure]
    #24699635 - 10/10/17 02:14 PM (6 years, 3 months ago)

if it's pins then you have a problem. it shouldn't pin unless fully colonised  I think the issue with BLI is it's prone to bacteria


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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OfflineXclozure
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Re: Official Copelandia/Panaeolus Thread [Re: tryptkaloids]
    #24699693 - 10/10/17 02:41 PM (6 years, 3 months ago)

It's from a MS Syringe tho. What's BLI.and yea im unsure idk i found 2 agar plates that sort of resembled those tiny white stones. they were on the bottom of the plates both times. both infected i believe.


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InvisibleShroomway
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Re: Official Copelandia/Panaeolus Thread [Re: Xclozure] * 1
    #24701653 - 10/11/17 09:05 AM (6 years, 3 months ago)

Pan Cyan Jamaica casing. No high hopes. Had a few of these not fruiting. This is however the best casing colonization I got so far. So never know! Fingers crossed


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InvisiblemushboyMDiscord
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Re: Official Copelandia/Panaeolus Thread [Re: Shroomway] * 1
    #24701916 - 10/11/17 10:47 AM (6 years, 3 months ago)

looks better than mine!



good luck.:cheers: i have hope dude.


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Re: Official Copelandia/Panaeolus Thread [Re: mushboy]
    #24702021 - 10/11/17 11:24 AM (6 years, 3 months ago)

Thanks Panarchist. I am waffling between liquid to mycobag vs wedge to grain to hpoo bags.

LC may be more 'reconnect friendy'. And my typical rgs expansion to oats not so much. Have excess rye so I expanded my current APE master over:



Did up 28 pasty plates this AM. Still some time for getting a couple methods in the works


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Re: Official Copelandia/Panaeolus Thread [Re: mushboy]
    #24702136 - 10/11/17 12:11 PM (6 years, 3 months ago)

Thanks Mushboy... One of these days!


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OfflinePanarchist
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Re: Official Copelandia/Panaeolus Thread [Re: Xclozure]
    #24703427 - 10/11/17 08:49 PM (6 years, 3 months ago)

Xclozure, BLI stands for blenderless liquid inoculate. it's a tek developed by a grower on the forum named Munchauzen. it involves adding a colonized plate to a sterile jar of water and shaking vigorously and then using that to inoculate your grain containers. definitely check it out if you're not familiar with it, it's a pretty popular method. it's an adaptation of a tek called LI(liquid inoculate) that involved taking a colonized plate or grainspawn and blending it with sterile water in a blender. LI doesn't seem to be as popular as it once was but some growers still seem to like it.

Shroomway, good luck with that mycelium. it looks like it has some potential.


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OfflineTheLostBoys
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Re: Official Copelandia/Panaeolus Thread [Re: Panarchist]
    #24703516 - 10/11/17 09:24 PM (6 years, 3 months ago)

just curious. what temps do pan cyan like to colonize at. I've got mine at like 78 degrees and it seems to be doing alright?


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OfflinePanarchist
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Re: Official Copelandia/Panaeolus Thread [Re: Panarchist]
    #24705026 - 10/12/17 03:11 PM (6 years, 3 months ago)

75-80F is the ideal range.


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