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Panarchist
Cyanescendant



Registered: 04/02/16
Posts: 432
Loc: Copeland
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Re: Official Copelandia/Panaeolus Thread [Re: Panarchist]
#24670255 - 09/29/17 02:05 PM (6 years, 3 months ago) |
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the difference between what looks good and what doesn't can be subtle so definitely keep an eye on that plate just be skeptical of it. and if you can get some mycelium going on WBS or other grains and then transfer it to agar you might get better results than going straight MS>agar. be aware though sometimes mycelium just doesn't like certain agar formulations and will have difficulty growing on them. for inoculating grains with MS three drops should be enough and a single drop on a plate should be enough. some growers recommend applying the spores in a streak pattern but i seem to get decent results just adding the drop to the center of the plate and not streaking. i don't think there's any advantage to inoculating near the edge as you can restrict your access to all sides of the growth and it's more likely that it could have some infection land on it.
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Mateja



Registered: 07/14/16
Posts: 7,948
Loc: Here
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Re: Official Copelandia/Panaeolus Thread [Re: Panarchist]
#24670386 - 09/29/17 02:42 PM (6 years, 3 months ago) |
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You are right near the edges is not recommended, I just wanted to get as many points of inoculation so that chances are bigger for germination, And of 16 places i inoculated only one shows growth, I guess we will have to wait and see what's growing. Can someone identify the mold that is growing? Or could this be pan mycelium?
What kind of mold grows these rings? I'm pretty sure pan myc grows rings like these
-------------------- Cakes inside Water Tub
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tryptkaloids
Learner



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Re: Official Copelandia/Panaeolus Thread [Re: Mateja]
#24671016 - 09/29/17 06:27 PM (6 years, 3 months ago) |
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I have never been able to germinate pan myc from a syringe. atleast not clean pan myc. pans and mold seem to like each other quite a bit IME. I'm pretty sure that's mold. as said above it should turn green or black because it will sporeulate, if it's pan myc it should be able to colonize that whole plat without changing color
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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RomeoPapa
Jackass of All Trades



Registered: 05/14/17
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Re: Official Copelandia/Panaeolus Thread [Re: tryptkaloids]
#24671196 - 09/29/17 07:28 PM (6 years, 3 months ago) |
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I know this is ass backwards, but could a person spawn with CVG and then case a pan cam shoebox with a cpoo casing after the sub colonized? I'm probably going to try it anyway since I've got the casing on the stove pasturizing.
I've also got enough cpoo/coir going to spawn a few shoe boxes proper.
Flamers, flame on!! Lol I love this forum.
-------------------- It's better to have it and not need it Than it is to need it and not have it.
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tryptkaloids
Learner



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Re: Official Copelandia/Panaeolus Thread [Re: RomeoPapa]
#24671206 - 09/29/17 07:32 PM (6 years, 3 months ago) |
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spawn with cvg? I'm confused
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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RomeoPapa
Jackass of All Trades



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Re: Official Copelandia/Panaeolus Thread [Re: tryptkaloids]
#24671244 - 09/29/17 07:52 PM (6 years, 3 months ago) |
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Here's a pan cam jar to spawn tomorrow
-------------------- It's better to have it and not need it Than it is to need it and not have it.
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RomeoPapa
Jackass of All Trades



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Re: Official Copelandia/Panaeolus Thread [Re: RomeoPapa]
#24671262 - 09/29/17 08:10 PM (6 years, 3 months ago) |
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I guess I'm going to case these with cpoo.
-------------------- It's better to have it and not need it Than it is to need it and not have it.
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cronicr



Registered: 08/07/11
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Re: Official Copelandia/Panaeolus Thread [Re: RomeoPapa]
#24671303 - 09/29/17 08:36 PM (6 years, 3 months ago) |
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Don't case with bulk
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Panarchist
Cyanescendant



Registered: 04/02/16
Posts: 432
Loc: Copeland
Last seen: 7 months, 16 days
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Re: Official Copelandia/Panaeolus Thread [Re: RomeoPapa]
#24671333 - 09/29/17 08:49 PM (6 years, 3 months ago) |
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i think you mean spawn to CVG. it can be done but from what i've seen you don't get as good results as when you use a manure-based substrate. using cpoo as a casing layer wouldn't be a good idea. a casing layer should be composed of things mycelium doesn't like to eat. and cpoo is so rich in minerals it can cause nutrient burn. peat/verm or jiffy seed starter mix are the most popular options. cpoo can be used as an additive in a substrate mix but it should not exceed %10 of the total amount from what i understand.
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RomeoPapa
Jackass of All Trades



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Re: Official Copelandia/Panaeolus Thread [Re: Panarchist]
#24671568 - 09/29/17 10:00 PM (6 years, 3 months ago) |
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Ok well I've got 5 qt jars of 80 or 90% cpoo cooling down now. I've got 3 jars of pan cam oat spawn at 100%.
I goofed and spawned 4 jars of pan cam to shoe boxes 4 days ago. 1 myco qt to one full qt jar of CVG. It's almost fully colonized so idk. I'm going to let it do it's 5hing. I will do better next time.
The good news is I've got a bunch of PE wheat grain jars coming along nicely!
Should I not spawn to such a high % of poo?
I'm rambling I guess. Too many barley pops tonight!
Gotta luv the shrlomedy! It saved my life
-------------------- It's better to have it and not need it Than it is to need it and not have it.
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tryptkaloids
Learner



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Re: Official Copelandia/Panaeolus Thread [Re: Panarchist]
#24672461 - 09/30/17 12:16 PM (6 years, 3 months ago) |
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Quote:
Panarchist said:. cpoo can be used as an additive in a substrate mix but it should not exceed %10 of the total amount from what i understand.
I've heard this but for chicken and horse poo. I've heard of people using up to a 40% cpoo sub with success.. that may have been a cube grow though...
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Xclozure
Stoned as shiiitakes


Registered: 09/09/12
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Re: Official Copelandia/Panaeolus Thread [Re: tryptkaloids]
#24672495 - 09/30/17 12:33 PM (6 years, 3 months ago) |
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So I was looking into doing a sub without manure I have some quart jars colonizing. Anyone have a specifc tek or recipe for me?
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Xclozure
Stoned as shiiitakes


Registered: 09/09/12
Posts: 421
Last seen: 1 year, 6 months
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Re: Official Copelandia/Panaeolus Thread [Re: Xclozure]
#24672526 - 09/30/17 12:52 PM (6 years, 3 months ago) |
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I still want the recipe/tek for the quarts. But I'm adding another question. I've never done pf tek, but could I do pan agar transfers to pint jars for cake? also do they have to be half pint or do you guys prefer pint jars??
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tryptkaloids
Learner



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Re: Official Copelandia/Panaeolus Thread [Re: Xclozure]
#24672548 - 09/30/17 01:04 PM (6 years, 3 months ago) |
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half pint because you want to birth them, every question you have asked can be found if you learn to use the search engine... link in my sig
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Xclozure
Stoned as shiiitakes


Registered: 09/09/12
Posts: 421
Last seen: 1 year, 6 months
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Re: Official Copelandia/Panaeolus Thread [Re: tryptkaloids]
#24672662 - 09/30/17 02:08 PM (6 years, 3 months ago) |
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I cant find a tek with a step by step without manure?
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Snazz
Polymath



Registered: 11/24/15
Posts: 1,584
Loc: Canada
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Re: Official Copelandia/Panaeolus Thread [Re: tryptkaloids]
#24672674 - 09/30/17 02:14 PM (6 years, 3 months ago) |
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After a few failed attempts last year, going to try Pans again. Learned my lesson about going straight to grain by wasting those strains.
Tempted to try azures again, but the climate here forces 100% indoor.
Grabbed a tropicalis instead. Will make up some growlogs when the time comes.
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Snazz
Polymath



Registered: 11/24/15
Posts: 1,584
Loc: Canada
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Re: Official Copelandia/Panaeolus Thread [Re: tryptkaloids]
#24672687 - 09/30/17 02:20 PM (6 years, 3 months ago) |
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Quote:
tryptkaloids said:
Quote:
Panarchist said:. cpoo can be used as an additive in a substrate mix but it should not exceed %10 of the total amount from what i understand.
I've heard this but for chicken and horse poo. I've heard of people using up to a 40% cpoo sub with success.. that may have been a cube grow though...
Chicken can give Nitrogen burn. Pretty potent fert. Other poo is fine in large %s but can get very compact without straw, verm etc. I found perlite actually helps to fluff it up in small amts
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Panarchist
Cyanescendant



Registered: 04/02/16
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Loc: Copeland
Last seen: 7 months, 16 days
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Re: Official Copelandia/Panaeolus Thread [Re: Xclozure]
#24672895 - 09/30/17 03:26 PM (6 years, 3 months ago) |
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Quote:
Xclozure said: So I was looking into doing a sub without manure I have some quart jars colonizing. Anyone have a specifc tek or recipe for me?
it seems like the most popular choice for manure-less substrates is straight coir or coir/verm/gypsum. check out boddhisatta's coir prep tek for a good instructional. from what i've seen in other people's grows it seems like manure-less substrates do not give as good results in fact i noticed a panaeolus grower in another group mention that when he used one it didn't even produce fruits.
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TheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,941
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Re: Official Copelandia/Panaeolus Thread [Re: tryptkaloids]
#24673100 - 09/30/17 04:41 PM (6 years, 3 months ago) |
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Quote:
Panarchist said:
Quote:
Xclozure said: So I was looking into doing a sub without manure I have some quart jars colonizing. Anyone have a specifc tek or recipe for me?
it seems like the most popular choice for manure-less substrates is straight coir or coir/verm/gypsum. check out boddhisatta's coir prep tek for a good instructional. from what i've seen in other people's grows it seems like manure-less substrates do not give as good results in fact i noticed a panaeolus grower in another group mention that when he used one it didn't even produce fruits.
The research I have done says Pan's don't do so well on CVG. I have found lots of info stating good results from a straw based substrate.
Quote:
tryptkaloids said: half pint because you want to birth them, every question you have asked can be found if you learn to use the search engine... link in my sig
Could use wide mouth FULL pints. I use them for cakes and they come out just fine. Half pints are not the only option.
-------------------- JOIN THE POW WOW
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Xclozure
Stoned as shiiitakes


Registered: 09/09/12
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Ok second thought I have access to a pile of aged manure mix between stable and field he said has pine shavings in it
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