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Fantastic Mr. Fox
Grass Seed Connoisseur


Registered: 02/12/15
Posts: 3,225
Loc:
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Re: Official Copelandia/Panaeolus Thread [Re: janica] 1
#22384776 - 10/15/15 06:04 PM (8 years, 3 months ago) |
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Nicee, janica!!

That black drop, is a water droplet that has spores in it. By the looks of the gills, it's sporelating. Better print him soon, before its too late.
I can tell tht your humidity is too high... The surface shouldnt have beads of water.. It should actually be drying.. Pans like it a bit more on the dry side. All the water they need is within the substrate. Stop misting after the pins/primordia have formed. && drop your humidity (70-80%)
Either way, Brava!!!
-------------------- Giving is all we have, for we're just grateful to be alive

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saralove



Registered: 10/01/13
Posts: 1,068
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helping us all with pans daily..i love it..thanks for what you do Mr. Fox.
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Listening to: emancipator - baralku tour (live) | AMU
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Fantastic Mr. Fox
Grass Seed Connoisseur


Registered: 02/12/15
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Re: Official Copelandia/Panaeolus Thread [Re: saralove] 1
#22384901 - 10/15/15 06:33 PM (8 years, 3 months ago) |
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-------------------- Giving is all we have, for we're just grateful to be alive

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janica



Registered: 05/05/15
Posts: 57
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I agree with saralove, thank you for helping! I didn´t mist, It´s just the water on the substrate and humidity, I´ll increase the air exchange. Thanks again Mr Fox
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janica



Registered: 05/05/15
Posts: 57
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Re: Official Copelandia/Panaeolus Thread [Re: saralove]
#22384973 - 10/15/15 06:50 PM (8 years, 3 months ago) |
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Quote:
saralove said: nice one janica 
nominated 
Thank you sara, they are so beautiful
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Darkhome
•Shaman•N•Training•



Registered: 07/10/15
Posts: 517
Loc: United States
Last seen: 7 years, 1 month
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Re: Official Copelandia/Panaeolus Thread [Re: janica] 1
#22386699 - 10/16/15 01:01 AM (8 years, 3 months ago) |
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Quote:
janica said: Panaeolus Cambodginiesis fruiting, just a few but I´m so happy!
 Do you guys know what is that black-bluish drop?
That's beautiful... It deserves a ninja turtle
-------------------- “The shaman is not merely a sick man, or a madman; he is a sick man who has healed himself.” ~Terence McKenna~ "NOTHING"...I post or say is "TRUE"...Any pictures are taken from the Web and any conversation is just research for a fictional character. That being said; He is constantly seeking knowledge and evolving.
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MacMerdin
Hunter



Registered: 04/26/12
Posts: 2,005
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Re: Official Copelandia/Panaeolus Thread [Re: oontribe]
#22386842 - 10/16/15 02:48 AM (8 years, 3 months ago) |
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Quote:
oontribe said:
another question i have read that cinctulus can form truffles...are they active? do they form alot of em?
They form blue/green psuedo-sclerotia.

Not sure if they are worth trying to save.
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Psilosopherr
A psilly goose



Registered: 02/15/12
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Re: Official Copelandia/Panaeolus Thread [Re: MacMerdin]
#22387697 - 10/16/15 10:05 AM (8 years, 3 months ago) |
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I'd never heard of psuedo sclerotia til the other day. I wonder if cloning could lead you to a 'strain' that would produce sclerotia normally. Imagine if we got a cube to do that...twould be sick
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firstTIMER420
Born the son of a sharecropper..



Registered: 05/08/15
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Re: Official Copelandia/Panaeolus Thread [Re: Psilosopherr]
#22387776 - 10/16/15 10:24 AM (8 years, 3 months ago) |
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Can someone post some recent pics of an agar plate after MS inoculation of Pan cyan spores, and a plate after rthe first or second transfer? I cant seem the culture to "thrive" it stays flat on the plate and doesn't really look like much, but its there. really starting to get annoying and Ive switched mediums so im not sure if its the agar blend. Ive also tried switching strain and source as well.
-------------------- 1 2 3 4 http://www.shroomery.org/forums/showflat.php/Number/22386794[/url] --Anything posted by this account is completely fictitious--
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Psilosopherr
A psilly goose



Registered: 02/15/12
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Re: Official Copelandia/Panaeolus Thread [Re: firstTIMER420]
#22387808 - 10/16/15 10:32 AM (8 years, 3 months ago) |
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cronicr posted a few pics somewhere in this thread.
I'm having the same problems though. I must've done 15-20 MS petri's and I only got one that looks and acts like mycelium. The rest would often stall or look funky. not contamed I don't think, just funky.
Its like the genetics simply have a good chance of spitting out a shitty strain or I'm just having bad luck. Pans are the king of stalling so far IME.
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Fantastic Mr. Fox
Grass Seed Connoisseur


Registered: 02/12/15
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Re: Official Copelandia/Panaeolus Thread [Re: Psilosopherr]
#22387827 - 10/16/15 10:37 AM (8 years, 3 months ago) |
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That's what I was suggesting yesterday..
They dont get very big at all.. && from my understanding only Panaeolus cambodginesis produces pseudosclerotia

Ola'h's 1968 paper describes certain Panaeolus species, also mentions "Pseudosclerotii" formation in the cultured mycelium of Panaeolus cambodginiensis.
If you have a sclerotia producing culture, your best bet would be to grow it out on Rye grass seed, or berries in order to get the most out of the culture.. Then, clone your biggest stone from the jar(s)
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Fantastic Mr. Fox
Grass Seed Connoisseur


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Re: Official Copelandia/Panaeolus Thread [Re: firstTIMER420]
#22387830 - 10/16/15 10:39 AM (8 years, 3 months ago) |
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Quote:
firstTIMER420 said: Can someone post some recent pics of an agar plate after MS inoculation of Pan cyan spores, and a plate after rthe first or second transfer? I cant seem the culture to "thrive" it stays flat on the plate and doesn't really look like much, but its there. really starting to get annoying and Ive switched mediums so im not sure if its the agar blend. Ive also tried switching strain and source as well.
Here ya go..

If you really want help you should post a picture of your dish.. No two cultures are the same... They're like snowflakes
-------------------- Giving is all we have, for we're just grateful to be alive

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Psilosopherr
A psilly goose



Registered: 02/15/12
Posts: 12,278
Last seen: 1 month, 8 days
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Quote:
Fantastic Mr. Fox said: That's what I was suggesting yesterday..
They dont get very big at all.. && from my understanding only Panaeolus cambodginesis produces pseudosclerotia

Ola'h's 1968 paper describes certain Panaeolus species, also mentions "Pseudosclerotii" formation in the cultured mycelium of Panaeolus cambodginiensis.
If you have a sclerotia producing culture, your best bet would be to grow it out on Rye grass seed, or berries in order to get the most out of the culture.. Then, clone your biggest stone from the jar(s)
yeah thats what got me started on it. I just had to repost it in my own words I guess
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Grey
⇜ ✯ ⇝



Registered: 11/06/14
Posts: 6,223
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Re: Official Copelandia/Panaeolus Thread [Re: Psilosopherr]
#22387869 - 10/16/15 10:51 AM (8 years, 3 months ago) |
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Pan. Subbalteatus (cinctulus) can produce pseudo sclerotia. Pan. Tropicalis has too, I believe.
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Fantastic Mr. Fox
Grass Seed Connoisseur


Registered: 02/12/15
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Re: Official Copelandia/Panaeolus Thread [Re: Grey]
#22387898 - 10/16/15 11:01 AM (8 years, 3 months ago) |
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Do you have any cited information by chance? (not trying to be a dick, just curious)
-------------------- Giving is all we have, for we're just grateful to be alive

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oontribe

Registered: 01/14/15
Posts: 3,570
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Iam sorry guys but what is pseudosclerotia? Can you drop it in a jar and start a new cinctulus jar? Like how you would do with atl7?
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Fantastic Mr. Fox
Grass Seed Connoisseur


Registered: 02/12/15
Posts: 3,225
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Re: Official Copelandia/Panaeolus Thread [Re: oontribe]
#22388035 - 10/16/15 11:49 AM (8 years, 3 months ago) |
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pseudosclerotia is false/fake sclerotia..
I haven't done it personally.. But you can try to inoculate new jars using sclerotia.. Be sure to post your results! It'll mean alot to the community.
I dont see why it wouldn't work though, since sclerotia is just 'mycelia mass'
-------------------- Giving is all we have, for we're just grateful to be alive

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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Quote:
Fantastic Mr. Fox said: Depends on the sp.
For Cyanescens 1.5g = 3.5g Cubensis For Cambodginesis 1g = 4-5g Cubensis. IMO/E
Pan's are smoother/cleaner.. Cubes just feel dirty to me.
Agreed 100%. And after you have eaten pans. Itsreally hard to eat cubes again. Its not even comparable in my eyes.
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oontribe

Registered: 01/14/15
Posts: 3,570
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Quote:
Fantastic Mr. Fox said: pseudosclerotia is false/fake sclerotia..
I haven't done it personally.. But you can try to inoculate new jars using sclerotia.. Be sure to post your results! It'll mean alot to the community.
I dont see why it wouldn't work though, since sclerotia is just 'mycelia mass'
This will take a couple of months before starting it...will share the info for sure. Thank you for the info man.
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Psilosopherr
A psilly goose



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Re: Official Copelandia/Panaeolus Thread [Re: eatyualive]
#22388205 - 10/16/15 12:44 PM (8 years, 3 months ago) |
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So how to you guys pronounce panaeolus? Pan-ayy-o-lus, pan-ee-o-lus, or ???
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