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OfflinePanarchist
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Re: Official Copelandia/Panaeolus Thread [Re: Bushbud]
    #24553242 - 08/14/17 01:29 PM (6 years, 5 months ago)

nice fruits, 99.99. keep up the good work.

Bushbud, good luck with your project. if you have any problems you need help with just hit up this thread.


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InvisibleBushbud
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Re: Official Copelandia/Panaeolus Thread [Re: Panarchist]
    #24554895 - 08/15/17 02:32 AM (6 years, 5 months ago)

Thanks mate. I can't wait to get my spores, I've never grown before or had any experience with anything but subaeruginosa . I have some subaeruginosa  mycelium growing at the moment and that's the entirety of my experience. I'm planning on growing panaeolus cyanescens on wallaby shit. Should be fun.


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InvisibleTheMadHatter420
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Re: Official Copelandia/Panaeolus Thread [Re: Bushbud]
    #24555215 - 08/15/17 08:22 AM (6 years, 5 months ago)

That sounds like an interesting grow, wallaby shit.


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InvisibleBushbud
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Re: Official Copelandia/Panaeolus Thread [Re: TheMadHatter420]
    #24556513 - 08/15/17 06:57 PM (6 years, 5 months ago)

Yeah plenty of it around my place. I'll have to document it.


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Offline99.99
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Re: Official Copelandia/Panaeolus Thread [Re: 99.99]
    #24559028 - 08/16/17 07:56 PM (6 years, 5 months ago)

Quote:

99.99 said:
Pan  tropicals
Just did my 2nd clone transfer of this cluster, 1st plates had a little bit of bacteria so I've had to put it  on antibacterial agar






Wow the clones i made of this cluster are growing about twice as fast as original culture


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OfflineTheBlackCat
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Re: Official Copelandia/Panaeolus Thread [Re: 99.99]
    #24559181 - 08/16/17 09:10 PM (6 years, 5 months ago)

Quote:

99.99 said:
Pan tropicals
Totally insane pin set ,every bit of white you see is a pin






I love the. They are cute. I would hug them all if they wouldn't turn into pulp. I'm so dang excited for this pan cyan tub of mine. I'm just praying it fruits. It looks so sweet. I don't think it's even been two days and I ckeep looking inside like it's going to fruit already. :Awemush: I totally have to stop looking at it. I saw some tiny water droplets today and panicked and thought I had mycogone. Dissected it with some tweezers and the fluid was clear and it smelled mushy. I always get paranoid when they get to this stage. Should wear off the more I grow.


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Edited by TheBlackCat (08/16/17 09:11 PM)


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Offlinehamloaf
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Re: Official Copelandia/Panaeolus Thread [Re: TheBlackCat]
    #24559185 - 08/16/17 09:13 PM (6 years, 5 months ago)

I like the looks of the Tropicallis too.  They kind of resemble long and skinny Ps Allenii, imo.


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InvisibleMycolorado
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Re: Official Copelandia/Panaeolus Thread [Re: hamloaf]
    #24559197 - 08/16/17 09:19 PM (6 years, 5 months ago)

:begger:


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Offlinehamloaf
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Re: Official Copelandia/Panaeolus Thread [Re: Mycolorado]
    #24561580 - 08/17/17 09:58 PM (6 years, 5 months ago)

Panaeolus Cyanescens "Texas" in LME LC @ 1 quarter tablespoon per quart of water.



Pan Cyan Texas LC aspired into a 140cc syringe.



The rest of the LC (about 10-20ccs) was free poured into a freshly sterilized, 5 quart bag of whole oats, then very lightly, and gently rolled around for a few seconds to distribute the liquid through out the grains evenly, as well as, create more inoculation points without over trumitizing the culture itself. 



Those Panaeolus Cyanescens "wild Tampa", and Cambodginiensis Goliath (Thailand) 140cc syringe LCs have tested good so far, 5 days from inoculation.

Cyanescens "Tampa".



Cambodginiensis Goliath (Thailand).



From my own personal experiance with LC, I know if these things were contaminated they'd have spit a mold culture all over that agar media by now.  Usually from about 5-6 days from inoculation of the agar if recovery of the mushroom mycelium has been established by then, then the culture is called good, and expanded upon.


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Offlinemushpunx
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Re: Official Copelandia/Panaeolus Thread [Re: hamloaf]
    #24562023 - 08/18/17 07:12 AM (6 years, 5 months ago)

Ive noticed that pan cultures are pretty slow, but once mine get to the size of a quarter, maybe between a quarter and a half dollar, they seem to slow down a lot- almost seems like they stall. Hard to tell though , I didn't note the time they started to slow some.

Is this typical?


Ham- thats good to know about LCs- question, I have a Pan Cyan LC in the fridge , tested really nice and clean on agar, but I might it might be toasted cause I accidentally bumped the inside of the neck of the flask with a syringe barrel- I talked to you about this before.

Should a new test on agar show if its clean or not?

the syringe bumped the inside of the neck so it didn't touch the LC, I'm worried if nothing fell into the culture it might get contaminated when I pour (if it isn't already ) when it touches that area. Not sure if it works like that or not.

I'm gunna test it on agar again but I'm worried it wont show


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InvisibleTheMadHatter420
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Re: Official Copelandia/Panaeolus Thread [Re: mushpunx]
    #24562046 - 08/18/17 07:30 AM (6 years, 5 months ago)

If you introduced a slight bit of bacteria but no mold, it would still kick ass being used for cakes. I personally have used LC with some bacteria and got wonderful cakes out of it. Since it is an unbroken colony the bacteria doesn't really do much harm.


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Offlinehamloaf
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Re: Official Copelandia/Panaeolus Thread [Re: mushpunx]
    #24562051 - 08/18/17 07:33 AM (6 years, 5 months ago)

Quote:

mushpunx said:
Ive noticed that pan cultures are pretty slow, but once mine get to the size of a quarter, maybe between a quarter and a half dollar, they seem to slow down a lot- almost seems like they stall. Hard to tell though , I didn't note the time they started to slow some.

Is this typical?


Ham- thats good to know about LCs- question, I have a Pan Cyan LC in the fridge , tested really nice and clean on agar, but I might it might be toasted cause I accidentally bumped the inside of the neck of the flask with a syringe barrel- I talked to you about this before.

Should a new test on agar show if its clean or not?

the syringe bumped the inside of the neck so it didn't touch the LC, I'm worried if nothing fell into the culture it might get contaminated when I pour (if it isn't already ) when it touches that area. Not sure if it works like that or not.

I'm gunna test it on agar again but I'm worried it wont show



If the cultures are stalling, mushies, that's a sign of infection.  My Panaeoluses all rip through the liquid media at different rates with some varities seeming to move faster than others, but all those observations are moot being that multispore geneticals are being worked with.  Only thing my LCs ripping through the medium is a testament to is the LC being clean.  Had a few stumbles, and bumbles when aspiring those first 2 large syringes, but it was the first time using syringes of that size.  The barrel knocked agains the side of the media vessel a few times, and once on one of the aspirations the needled touched the side of the media vessel as the syringe was being pulled from the media due to being full.  Usually confidence is had with LC, but, because those negative events happened the decision was made to test the LC.  Did you return the needle back into the culture with out flame sterilizing it after you bumped the side of the flask with the needle, and was the needle freshly flame sterilized right before bumping the flask??  If that were me, and my needle tapped the side of the media vessel gears automatically get switched, and the needle goes back into the flame before proceeding again.  Testing it again is good idea if you are unsure.  If you really want to know the truth, then swirl the liquid media around until where the liquid makes contact with the spot you tapped with the needle.  Let the media sit for a second, then pull another testing sample.  If there is an infection present, it will get the oxygen it needs to germinate once it leaves the syringe, and in the agar media vessel, as well.


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Offlinetryptkaloids
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Re: Official Copelandia/Panaeolus Thread [Re: hamloaf]
    #24562618 - 08/18/17 11:49 AM (6 years, 5 months ago)

I think he bumped the plastic part :shrug:


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Offlinemushpunx
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Re: Official Copelandia/Panaeolus Thread [Re: tryptkaloids]
    #24563252 - 08/18/17 03:58 PM (6 years, 5 months ago)

Yea I had a 4" needle on but still had to tilt the flask to suck up some culture (usually I pour some into a sterile shotglass first because these flasks are so deep) and I clumsily over reached and touched the end of the plastic barrel to the inside of the flask :facepalm:


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OfflineTheBlackCat
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Re: Official Copelandia/Panaeolus Thread [Re: mushpunx]
    #24563470 - 08/18/17 05:41 PM (6 years, 5 months ago)

I'm officially depressed. I think my pans are sick with mycogone. After the week I had I'm not happy. I guess I was right when I first suspected infection and not paranoid. Just in case I took some photos since I have never grown pans and it could be part of normal development which I highly doubt After ripping up several suspicious clumps I figured I'd stop and ask you guys. After my trouble with my oysters this is filling me with so much sadness.







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Invisiblemynakedrat
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Re: Official Copelandia/Panaeolus Thread [Re: TheBlackCat]
    #24563478 - 08/18/17 05:44 PM (6 years, 5 months ago)

Y not fruit and see?


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OfflineTheBlackCat
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Re: Official Copelandia/Panaeolus Thread [Re: mynakedrat]
    #24563488 - 08/18/17 05:48 PM (6 years, 5 months ago)

Because I just want to see if it is. Because I may be able to at least slow it down. I just don't want to tear up clumps if I don't have to. I'm highly suspicious because they do seem like pitted brain like areas. I want to remove them because the infection once it gets bad will just leave me with a tub of pitted brain fungus. I was wondering if I could salt the spots I see or something like that.


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InvisibleGerms
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Re: Official Copelandia/Panaeolus Thread [Re: TheBlackCat]
    #24563514 - 08/18/17 06:02 PM (6 years, 5 months ago)

That belongs outside, if not at least placed in a different area, best case in the garbage.

Honestly I was pretty skeptical when you had said it had colonized in 2-3 days but didn't wanna seem like a condescending douchebag like I usually am. IMO, you didn't transfer away enough from the original culture. I know we started pans on the same day and I just put mine to grains this week.

I'd go back to the original culture that I believe you said you still have, and put it to grains you prepped yourself instead of a bag.

You're on the right track


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Offline99.99
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Re: Official Copelandia/Panaeolus Thread [Re: TheBlackCat]
    #24563534 - 08/18/17 06:11 PM (6 years, 5 months ago)

Quote:

TheBlackCat said:
I'm officially depressed. I think my pans are sick with mycogone. After the week I had I'm not happy. I guess I was right when I first suspected infection and not paranoid. Just in case I took some photos since I have never grown pans and it could be part of normal development which I highly doubt After ripping up several suspicious clumps I figured I'd stop and ask you guys. After my trouble with my oysters this is filling me with so much sadness.










Ug i wonder if thats what i was getting evertime i tried to grow, The Jamaicans or Hawaiian pans
Mine looked just like this and would never fruit
  The tropicals im doing now look nothing like The cultures from those two and being a pan variety they probably should look similar

This is the sub of Jamaican before I cased it
this would grow right through the casing also so I'm thinking I have had the same infection you do



Edited by 99.99 (08/18/17 06:14 PM)


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OfflineTheBlackCat
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Re: Official Copelandia/Panaeolus Thread [Re: Germs]
    #24563553 - 08/18/17 06:18 PM (6 years, 5 months ago)

Mycogone grows on fruiting bodies. It was probably the casing layer since mycogone lives in soil. At least my purple mystics look good. I'm dumping this in the backyard before it sporulates everywhere.


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