Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Original Sensible Seeds High THC Strains   Left Coast Kratom Buy Kratom Extract   Kraken Kratom Red Vein Kratom   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Mushroom-Hut Liquid Cultures   North Spore Cultivation Supplies

Jump to first unread post Pages: < First | < Back | 169 | 170 | 171 | 172 | 173 | 174 | 175 | 176 | 177 | 178 | 179 | 180 | 181 | 182 | 183 | 184 | 185 | 186 | 187 | 188 | 189 | Next > | Last >
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlinehamloaf
Loaf of Fam.
Male User Gallery

Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma. Flag
Last seen: 1 year, 8 months
Trusted Cultivator
Re: Official Copelandia/Panaeolus Thread [Re: 99.99]
    #24526466 - 08/03/17 12:58 AM (6 years, 5 months ago)

Good job, brother.  :super:
I'd up that fresh air exchange a little bit.  The feet are getting fuzzy.


--------------------

   
How I Get Stuff done. - My Reference Guide. - My Grows.


Extras: Filter Print Post Top
Offline99.99
Stranger
 User Gallery

Registered: 12/22/14
Posts: 1,933
Last seen: 20 minutes, 50 seconds
Re: Official Copelandia/Panaeolus Thread [Re: hamloaf]
    #24526473 - 08/03/17 01:02 AM (6 years, 5 months ago)

Cool ill do that


Extras: Filter Print Post Top
InvisibleShroomway
It's me!
Male


Registered: 07/05/17
Posts: 438
Re: Official Copelandia/Panaeolus Thread [Re: 99.99]
    #24526771 - 08/03/17 06:45 AM (6 years, 5 months ago)

Looks very prolific! Well done indeed :smile:

Question: as I am having difficulties fruiting Pan Cyan I am trying all kinds of things. The result is that I am doing quite a bit of agar transfers. Will this eventually result in an isolated strain? Or at least less sub strains in my culture? And will this (one or the other) improve the quality of the culture in the sense that there will be less competition between sub strains during colonizing?

Btw: I understand that Pan Cyan isn't as rhizomorphic as cubes are and displays less sectoring... So its harder to visually select sub strains. I also understand that selecting a substrain doesn't mean it has favorable characteristics over the multispore you started with. It obviously depends on the genetics of the substrain you're talking about.

But will the agar transfers help? And does it help to alway's take the smallest myc transfer you can technically achieve?


Extras: Filter Print Post Top
Offline99.99
Stranger
 User Gallery

Registered: 12/22/14
Posts: 1,933
Last seen: 20 minutes, 50 seconds
Re: Official Copelandia/Panaeolus Thread [Re: Shroomway]
    #24527350 - 08/03/17 11:53 AM (6 years, 5 months ago)

Quote:

Shroomway said:
Looks very prolific! Well done indeed :smile:

Question: as I am having difficulties fruiting Pan Cyan I am trying all kinds of things. The result is that I am doing quite a bit of agar transfers. Will this eventually result in an isolated strain? Or at least less sub strains in my culture? And will this (one or the other) improve the quality of the culture in the sense that there will be less competition between sub strains during colonizing?

Btw: I understand that Pan Cyan isn't as rhizomorphic as cubes are and displays less sectoring... So its harder to visually select sub strains. I also understand that selecting a substrain doesn't mean it has favorable characteristics over the multispore you started with. It obviously depends on the genetics of the substrain you're talking about.

But will the agar transfers help? And does it help to alway's take the smallest myc transfer you can technically achieve?




What I've done is not normal, normally for cubes I do tons of agar transfers to get an isolate
With this tropicals i dont think rhizomorphic agar is possible
My agar is just multi spore and about one transfer.
What I looked for it and my agar were actually pins and the ability of the mycelium to fruit, for some reson PDA agar worked best for the initial multi spore playes
The 2nd transfers wear to MEA

You can see in this photo small dark spots that are pins,  I just tried to propagate that


Extras: Filter Print Post Top
InvisibleShroomway
It's me!
Male


Registered: 07/05/17
Posts: 438
Re: Official Copelandia/Panaeolus Thread [Re: 99.99]
    #24527639 - 08/03/17 02:25 PM (6 years, 5 months ago)

What did you use to tape the petri dishes? I love to hear that u used cling wrap, cause where I live paratape is more than $40 a small role.. .

My myc seem to stall after some time. All kinds of agar recipes I used.. Oats agar works best for me till now... I use cling wrap. I reckon that could be the reason for the myc to stall? I like to try your method for finding a favorable piece of myc. Would love to see a pin in my agar and be able to transfer it...

At what temp do you keep your plates? Thanks :smile:


Extras: Filter Print Post Top
Offline99.99
Stranger
 User Gallery

Registered: 12/22/14
Posts: 1,933
Last seen: 20 minutes, 50 seconds
Re: Official Copelandia/Panaeolus Thread [Re: Shroomway]
    #24527742 - 08/03/17 03:12 PM (6 years, 5 months ago)

I kinda do a pasty plate but not really
My agar comes from fungi-perfecti premixed
I add water blend it in a blender, pour into thease containers and pressure cook them for 45 minutes
Biglots 3pcs 1.80


If i know their going to grain i just put them in ziplock bags while infront of flowhood just to keep them sterile and i use a full agar dish to my grain, i mix it up into little pcs and scoop intomy grain
I keep my tubs and agar dishes at 80 degrees
I don't wrap them with parafilm or saran wrap or anything they are good like they are

On the previous page there is the description of how I do my grain also


Edited by 99.99 (08/03/17 05:52 PM)


Extras: Filter Print Post Top
OfflineSpeed Racer
Stranger
I'm a teapot

Registered: 04/28/17
Posts: 102
Loc: Chicago
Last seen: 5 years, 4 months
Re: Official Copelandia/Panaeolus Thread [Re: 99.99]
    #24529697 - 08/04/17 11:19 AM (6 years, 5 months ago)

Nice Going 99


Extras: Filter Print Post Top
InvisibleShroomway
It's me!
Male


Registered: 07/05/17
Posts: 438
Re: Official Copelandia/Panaeolus Thread [Re: 99.99]
    #24529702 - 08/04/17 11:21 AM (6 years, 5 months ago)

Thanks a lot for your info 99 :smile::)


Extras: Filter Print Post Top
InvisibleGoldMold
Stranger in the night
Male User Gallery

Registered: 10/13/16
Posts: 737
Re: Official Copelandia/Panaeolus Thread [Re: Shroomway]
    #24529749 - 08/04/17 11:55 AM (6 years, 5 months ago)

I have some pan tropical on agar, I'm gonna be putting to oats soon. Exciting stuff!:borat:


Extras: Filter Print Post Top
OfflineSpeed Racer
Stranger
I'm a teapot

Registered: 04/28/17
Posts: 102
Loc: Chicago
Last seen: 5 years, 4 months
Re: Official Copelandia/Panaeolus Thread [Re: GoldMold]
    #24529972 - 08/04/17 02:16 PM (6 years, 5 months ago)

Hey there 99, could you share where you got your spores from? Did you start with a spore syringe from the Spore Works? I see they have Pan Tropicals.


Extras: Filter Print Post Top
Invisibleeatyualive
Eat's You Alive :)
 User Gallery


Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
Trusted Cultivator
Re: Official Copelandia/Panaeolus Thread [Re: Speed Racer]
    #24530032 - 08/04/17 02:47 PM (6 years, 5 months ago)

for reference 99.99. mature pan trops look like this.

Pan. Trops.



--------------------
EAT GETS SHIT DONE


:flame::chief:JOIN THE POW WOW:chief::flame:


Extras: Filter Print Post Top
Offline99.99
Stranger
 User Gallery

Registered: 12/22/14
Posts: 1,933
Last seen: 20 minutes, 50 seconds
Re: Official Copelandia/Panaeolus Thread [Re: eatyualive]
    #24530158 - 08/04/17 03:31 PM (6 years, 5 months ago)

Quote:

eatyualive said:
for reference 99.99. mature pan trops look like this.

Pan. Trops.







Yep Mine are to that stage so all harvest a bunch of them Saturday maybe try some clones


Extras: Filter Print Post Top
Offline99.99
Stranger
 User Gallery

Registered: 12/22/14
Posts: 1,933
Last seen: 20 minutes, 50 seconds
Re: Official Copelandia/Panaeolus Thread [Re: 99.99]
    #24531442 - 08/05/17 01:33 AM (6 years, 5 months ago)

Tommorow is harvest time, im also going to try some clones of those clustes



I have to say only one tube of the 8 has grown like mad so im really hoping cloning with give me a great start


Extras: Filter Print Post Top
InvisibleLemurLemur
Pray for Boog
 User Gallery


Registered: 01/30/17
Posts: 6,004
Loc: Drinking on the roof Flag
Re: Official Copelandia/Panaeolus Thread [Re: 99.99]
    #24531465 - 08/05/17 01:49 AM (6 years, 5 months ago)

What's up with those black caps??


--------------------
(when my data is fast play Lemur in chess at chess.com)[ [gradient:#D40B29,#18C418]Any1 expecting a trade from me i havent forgot about you pinky promise, i fr promise shits just shit rt now[/gradient]


Extras: Filter Print Post Top
Offline99.99
Stranger
 User Gallery

Registered: 12/22/14
Posts: 1,933
Last seen: 20 minutes, 50 seconds
Re: Official Copelandia/Panaeolus Thread [Re: LemurLemur]
    #24531468 - 08/05/17 01:53 AM (6 years, 5 months ago)

Quote:

LemurLemur said:
What's up with those black caps??




Aborts water got driped on them from the lid yesterday when i looked at them
Any water that gets on the pans aborts them and almost melts The mycelium,
When this pan was done I purposely sprayed it to see what it would do



Extras: Filter Print Post Top
InvisibleLemurLemur
Pray for Boog
 User Gallery


Registered: 01/30/17
Posts: 6,004
Loc: Drinking on the roof Flag
Re: Official Copelandia/Panaeolus Thread [Re: 99.99]
    #24531500 - 08/05/17 02:25 AM (6 years, 5 months ago)

Oh fuck...that is something to keep in mind, ik misting caused aborts but shit...


--------------------
(when my data is fast play Lemur in chess at chess.com)[ [gradient:#D40B29,#18C418]Any1 expecting a trade from me i havent forgot about you pinky promise, i fr promise shits just shit rt now[/gradient]


Extras: Filter Print Post Top
Offlinemushpunx
Fungus Punk
 User Gallery

Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 8 hours
Trusted Cultivator
Re: Official Copelandia/Panaeolus Thread [Re: 99.99]
    #24531668 - 08/05/17 05:00 AM (6 years, 5 months ago)

Quote:

99.99 said:
Quote:

LemurLemur said:
What's up with those black caps??




Aborts water got driped on them from the lid yesterday when i looked at them
Any water that gets on the pans aborts them and almost melts The mycelium,
When this pan was done I purposely sprayed it to see what it would do






This is crazy!


--------------------

Amateur Mycologists United
AMU Q&A


Extras: Filter Print Post Top
InvisibleInspir3
Aspir3 to Inspir3


Registered: 07/06/17
Posts: 234
Re: Official Copelandia/Panaeolus Thread [Re: mushpunx]
    #24535471 - 08/06/17 07:33 PM (6 years, 5 months ago)

how does one control moisture if you cant mist if i might ask.


--------------------


Extras: Filter Print Post Top
Offline99.99
Stranger
 User Gallery

Registered: 12/22/14
Posts: 1,933
Last seen: 20 minutes, 50 seconds
Re: Official Copelandia/Panaeolus Thread [Re: Inspir3]
    #24535489 - 08/06/17 07:40 PM (6 years, 5 months ago)

Quote:

Inspir3 said:
how does one control moisture if you cant mist if i might ask.




I put mine in small tubs and put them in a martha greenhouse with a cool mist  humidifier
That way they can get plenty of humid air with out getting wet


Extras: Filter Print Post Top
InvisibleInspir3
Aspir3 to Inspir3


Registered: 07/06/17
Posts: 234
Re: Official Copelandia/Panaeolus Thread [Re: 99.99]
    #24535505 - 08/06/17 07:48 PM (6 years, 5 months ago)

Quote:

99.99 said:
Quote:

Inspir3 said:
how does one control moisture if you cant mist if i might ask.




I put mine in small tubs and put them in a martha greenhouse with a cool mist  humidifier
That way they can get plenty of humid air with out getting wet




But what if you did cakes to trays in a sgfc? I guess thats the real question i have.


--------------------


Extras: Filter Print Post Top
Jump to top Pages: < First | < Back | 169 | 170 | 171 | 172 | 173 | 174 | 175 | 176 | 177 | 178 | 179 | 180 | 181 | 182 | 183 | 184 | 185 | 186 | 187 | 188 | 189 | Next > | Last >

Shop: Original Sensible Seeds High THC Strains   Left Coast Kratom Buy Kratom Extract   Kraken Kratom Red Vein Kratom   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Mushroom-Hut Liquid Cultures   North Spore Cultivation Supplies


Similar ThreadsPosterViewsRepliesLast post
* The official Psilocybe natalensis thread
( 1 2 3 4 ... 515 516 )
Hillbillyjohn 328,169 10,311 01/29/24 01:51 PM
by 3.A.M
* Official Misc EXOTIC Thread
( 1 2 3 4 ... 132 133 )
Jakeoncid419 110,038 2,647 01/29/24 01:07 PM
by Pnin
* Cheap shelves for a martha. Crill 1,882 16 12/24/08 10:59 AM
by phildurtz
* Magash, how much weight can your shelves bear? AnonymousNewbie 794 2 12/01/04 01:17 PM
by Magash
* Martha shelves (yeah I know it has been covered) Smushroom 1,039 4 10/05/05 12:06 PM
by searcher745
* Vermicuite pulled from US shelves? Freefly 499 1 06/06/01 04:39 PM
by egghead
* Rye-Z-Morph Bag for Panaeolus (Copelandia) Cyanescens? rob_531 4,312 12 01/10/05 12:33 PM
by agar
* How far apart should shelves be ? Nexxiss 925 1 06/24/04 12:49 AM
by matts

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
924,060 topic views. 37 members, 243 guests and 27 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.026 seconds spending 0.008 seconds on 15 queries.