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TheDuder
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Registered: 11/07/16
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Re: Official Copelandia/Panaeolus Thread [Re: TheDuder]
#24450352 - 07/01/17 06:12 PM (6 years, 6 months ago) |
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Im thinking about trying mini mono tubs for the jars, and using straight coir like I do with cubes. Does anyone know if shoeboxes would work for Pan combo too?
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|-------------------[Ps. Azurescens]------------------------------------------[Ps. Semilanceata]--------------------------------------------[Ps. Allenii]------------------------|
|--------------[Ps. Ovoideocystidiata]------------------------------------------[Ps. Stuntzii]--------------------------------------------[Ps. Baeocystis]----------------------|
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hamloaf
Loaf of Fam.


Registered: 12/23/09
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Re: Official Copelandia/Panaeolus Thread [Re: TheDuder]
#24450421 - 07/01/17 06:45 PM (6 years, 6 months ago) |
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Quote:
TheDuder said: Yeah I decided against shaking the jars. Last time I tried the pan cambo I shook and it never recovered. 
Pan myce doesnt like to rejoin is all. Sometimes it will, sometimes it won't. Whether or not pan myce rejoins is random. That's the reason why pan myce appears wispy. The solution would be to catch a single sector isolate, or a culture that's observed re joining on agar.
Shoe boxes are perfect for pans, because, again, they are not like cubes in a sense to where you can get bigger fruits from a larger substrate. Compared to cubes, pans are small fruits. The only way to get large fruits with pans is to clone a large pan fruit.
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TheDuder
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Re: Official Copelandia/Panaeolus Thread [Re: hamloaf]
#24450423 - 07/01/17 06:47 PM (6 years, 6 months ago) |
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Maybe ill just do shoeboxes then, do I need to case it or can I just pseudo case it at spawn?
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|-------------------[Ps. Azurescens]------------------------------------------[Ps. Semilanceata]--------------------------------------------[Ps. Allenii]------------------------|
|--------------[Ps. Ovoideocystidiata]------------------------------------------[Ps. Stuntzii]--------------------------------------------[Ps. Baeocystis]----------------------|
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hamloaf
Loaf of Fam.


Registered: 12/23/09
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Re: Official Copelandia/Panaeolus Thread [Re: TheDuder]
#24450432 - 07/01/17 06:52 PM (6 years, 6 months ago) |
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Both. With pans, case case case. A thin casing of about a quarter of an inch thick. I like to use 50/50 casing mix. Pans are fast enough that I don't see a reason to add the lime or gypsum to the casing. Careful with the misting, though. Only mist to keep the casing moist. Don't over mist and pan out the casing. Once pins arrive cut the misting, or the pins will most likely abort. Pans are fast. One more thing, let me know how using coir for Pans works out for you. As far as I know, Pans are synonmous with manure meaning they must be grown off of a manure based substrate. They fruit well on pasteurized wheat straw alone, or a 30/70 mix of dung, and straw. Mexicanas work well on coir, or coir-based substrates.
Edited by hamloaf (07/02/17 12:56 AM)
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TheMadHatter420
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Re: Official Copelandia/Panaeolus Thread [Re: hamloaf] 1
#24456767 - 07/04/17 12:39 PM (6 years, 6 months ago) |
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I got a Pan cambo agar plate to pin by casing it!!! WILL CLONE
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hamloaf
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Interesting!
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TheMadHatter420
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Re: Official Copelandia/Panaeolus Thread [Re: hamloaf] 1
#24456821 - 07/04/17 12:52 PM (6 years, 6 months ago) |
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This is the 2nd culture I made this work with Ham. I take my transfers and case after. I use a mix of coarse verm and some perlite. Just a hand full of each thrown in a bowl and hydrated. Give it a try. You at the least know that is a fruiting culture and probably the fastest on the plate. The Z pin I got like this was rather clean and gave some beautiful mycelium growth.
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hamloaf
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I probably will. I see you'll have to use a media vessel deep enough to be able to apply a casing, so squat jars and Petri dishes are out. Looks Iike glad mini rounds, and brf cake jars are in. Did you apply the casing in sterile conditions, then put the lid back on until the culture pinned?
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TheMadHatter420
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Re: Official Copelandia/Panaeolus Thread [Re: hamloaf]
#24456864 - 07/04/17 01:06 PM (6 years, 6 months ago) |
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Honestly no! It was a jar that was fully colonized, I just opened it, put the verm/perlite casing on and closed back up. Only opened every couple days to check progress. I have a Lucy in one of these wide mouth half pints as well as a mini round, both cased. Both should work fine. I think the thickness of the agar should be taken into consideration.
In the future I intend to make it a bit thicker for doing this for more nutrition. The 2 Lucy plates are very thin so do not know if they will pin. Then again the Z strain was not that thick nor this one. I think the key is case a plate that has not sat around for a really long time.
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TheMadHatter420
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Ham this was the Pan. cambo jar I cased. It was growing up the sides. The transfers I took actually came off of the glass. They were not "needed" transfers but their vigor was impressive and well bacteria will not grow up glass so I wanted to try it. I used a flamed and cooled scalpel to push the mycelium back down the sides of the jar before casing.
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hamloaf
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Very nice work, Mad. Was just considering last night leaving my pan cultures on agar to colonize until pins form. Unfortunately the culture is in Petri dishes, so I'd have to make some no pours to try this out. I have plenty of 8 ounce wide months though.
I would think the moisture content of the agar would be an important factor in how good casing agar would work, so it stands to reason that thicker agar would work better, because it has more moisture reserves than a thinner layer of agar. Not too sure nutrition comes into play with pinning strategy.
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TheMadHatter420
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Re: Official Copelandia/Panaeolus Thread [Re: hamloaf]
#24456936 - 07/04/17 01:30 PM (6 years, 6 months ago) |
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Could you "dub tub" the petri dish with another bottom plate so they line up, tape with MP or what ever? I only cased like 1/8th to a 1/4 inch.
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TheMadHatter420
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I want to work on this. I am thinking maybe a 1/2 inch of agar made with 2g/100ml LME. I would like to try to get some better fruiting conditions. If we could get them to at least pin similar to a normal grow we could see possible clusters for better choice of cloning material. So far I have only gotten single pins but I will mess around with this to try to get some pin clusters.
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tryptkaloids
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Quote:
TheMadHatter420 said: Could you "dub tub" the petri dish with another bottom plate so they line up, tape with MP or what ever? I only cased like 1/8th to a 1/4 inch.
why would you do that?
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TheMadHatter420
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Re: Official Copelandia/Panaeolus Thread [Re: tryptkaloids]
#24457340 - 07/04/17 04:27 PM (6 years, 6 months ago) |
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to be able to try casing that plate for pins.
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tryptkaloids
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why not just use the lid is what I'm saying. should have plenty of fae. just a micropore hole should do fine
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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TheMadHatter420
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Re: Official Copelandia/Panaeolus Thread [Re: tryptkaloids]
#24457393 - 07/04/17 04:44 PM (6 years, 6 months ago) |
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Ham is thinking there would not be enough room left after casing.
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TheMadHatter420
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More cased Pan cambo plate goodness. A couple more pins and what looks like a shit load of knots/primordia.
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hamloaf
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Are you taking any provisions to cleanliness with the vermiculite, or are you merely applying it to the agar fresh out of the bag?
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TheMadHatter420
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Re: Official Copelandia/Panaeolus Thread [Re: hamloaf]
#24460733 - 07/06/17 12:11 AM (6 years, 6 months ago) |
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Fresh out of the bag. This was not expected to work! In the future I will take are to sterilize the verm/perlite mix in the jar in the PC and transfer to agar plate in my SAB. This will be an attempt to kill, or at least weaken, any bacteria or mold spore chilling in the verm/perlite.
This has my mind opened up to a whole new way to work with MS. I figure there should still be a good mix of genetics in pins of off agar, I could be wrong. I still do not know a lot about how a mushroom is actually made up of many different strains.
I have been sharing this in the hopes that a few people will jump on board, with their old plates, in an attempt to research this. BUT with all the drama on the boards this is getting overlooked by most people. I really have thought this would grab more attention. I know I am no ace grower and it is not a tub full of big fruits, but this is still something rather exciting and very curious. I doubt many people have done this. I have found little post about it and nothing on anyone doing this to a Pan.
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