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OfflineSpeed Racer
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Registered: 04/28/17
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Re: Official Copelandia/Panaeolus Thread [Re: Teemo 6T3]
    #24414582 - 06/18/17 09:20 AM (6 years, 7 months ago)

These are NOT Tsetse Flies, I was just adding a little color to the post. I googled  Tsetse Flies to get the correct spelling and the definition says they are large, these flies a very small like a gnat or a fruit fly. 

I did take a look under the black poly and see it is colonizing however there are a couple spots of slimy looking growth as well. I'm going to move this tub to my shed. The temperatures in Chicago are ideal for growing right now. I need to leave town today and will take another look and some pictures on Tuesday. I'm not going open this tub up in my main growing room.

I'm going to start over from the agar to agar transfers I made 7 days ago that are looking good. Looking back I see where I could have introduced contaminate to these first grain jars and I will avoid that on the second go around.


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Invisibled0urd3n
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Re: Official Copelandia/Panaeolus Thread [Re: Speed Racer]
    #24416018 - 06/18/17 07:38 PM (6 years, 7 months ago)

Panaeolus cinctulus pseudo-scelerotia






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Offlinetryptkaloids
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Re: Official Copelandia/Panaeolus Thread [Re: d0urd3n]
    #24416126 - 06/18/17 08:22 PM (6 years, 7 months ago)

I would love to see someone isolate a pan stone worth growing out within my lifetime


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"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
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InvisibleTeemo 6T3
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Re: Official Copelandia/Panaeolus Thread [Re: tryptkaloids]
    #24416287 - 06/18/17 09:30 PM (6 years, 7 months ago)

Just spawned 3 tubs, got 2 more to go which i do tomorrow.

As i was gonna case my flower pots, i saw a couple of pins popping out and also lots of knots, went ahead and sprinkled casing material slightly ontop of it, hopefully it won't stall it, even if it did, im sure the knots will develop more :awedance:


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Shrooming Is Of The Essence


Rest In Peace Dankington


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InvisibleTeemo 6T3
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Re: Official Copelandia/Panaeolus Thread [Re: Teemo 6T3]
    #24419158 - 06/19/17 10:13 PM (6 years, 7 months ago)

Finished spawning 6 tubs of Pan cyans, 1 of them sandose, and the other 5 are Pan Jamaicans


They were slightly bacterial, but im sure they'll do fine.

I realised that everytime i shake them they start to stall and show some signs of wet spot, so next time i'll leave them be and not shake.


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Shrooming Is Of The Essence


Rest In Peace Dankington


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InvisiblemushboyMDiscord
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Re: Official Copelandia/Panaeolus Thread [Re: Teemo 6T3]
    #24419266 - 06/19/17 11:15 PM (6 years, 7 months ago)

your home looks peaceful.:stoned:


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Invisibled0urd3n
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Re: Official Copelandia/Panaeolus Thread [Re: mushboy]
    #24419317 - 06/19/17 11:41 PM (6 years, 7 months ago)

Teemo I would bet they were bacterial before the shake. Pans shake just fine for me. Could be wrong.


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Offlinehamloaf
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Re: Official Copelandia/Panaeolus Thread [Re: d0urd3n]
    #24419767 - 06/20/17 07:30 AM (6 years, 7 months ago)

Quote:

d0urd3n said:
Teemo I would bet they were bacterial before the shake. Pans shake just fine for me. Could be wrong.



This is an expample of. "THE RANDOM FACTOR".  What works for one doesn't always work for another.  I have always had problems after the shake with Panaeolus, so next run with the Pans I am going to employ methods that require the least amount of shaking.  LC-----> bag -----> substrate. 

The reason for a likely hood to stall after being shook with Panaeolus isn't because the myce is weak.  Contrary.  Pan myce, despite it's wispy appearance, is some strong stuff.  It is because with multi spore, Pan's strains, or sub strains don't like to rejoin with each other, so it's best to let them go only shaking once.  Just enough to get them out of the jar.  Cubes don't mind rejoining sub strains, so can be shaken all day. 

Somebody up thread suggested taking at least 5 transfers before going to grain.  That, to me, seems like plausible solution.  Seems like getting a Pan culture down to a few,  or single sectors would be a beneficial tool.  I'm going to try these suggestions.  I have plenty of T3 culture to play with.


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How I Get Stuff done. - My Reference Guide. - My Grows.


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InvisibleTeemo 6T3
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Re: Official Copelandia/Panaeolus Thread [Re: mushboy]
    #24420016 - 06/20/17 09:36 AM (6 years, 7 months ago)

Quote:

mushboy said:
your home looks peaceful.:stoned:



Thanks bro haha  :drugnerd:

d0urd3n, true true, but i didn't see any signs of it before the shake, they looked really healthy.

I think Hammington has a point, cus after the shake they really seemed to not rejoin.

But still, i think your right d0urd3n, cus the first Sandose grow i did, i shook it and it colonised perfectly without any trouble.

As Hammington said, Pan mycelium sure is strong, one of my tubs is already nearly fully colonised lol, and i spawned that jar on the 19th lol, so its been just 48 hours :omgz:


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Shrooming Is Of The Essence


Rest In Peace Dankington


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InvisibleJHOVA
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Re: Official Copelandia/Panaeolus Thread [Re: mushpunx]
    #24420087 - 06/20/17 10:13 AM (6 years, 7 months ago)

Quote:

mushpunx said:
Quote:

Elegua said:
Is anyone here growing Panaeolus using coir alone as the bulk substrate?  If so, how'd it do?  Is this a species that needs manure and such?





I'm curious myself. I've been told it does OK on coir.

Its difficult for me to get manure. When I get back in town in a couple weeks I'm going to spawn these Pan jars, I'm probably putting mine to Straw and casing them, but I'm going to do one on CVG just to see.





My pan goliath is on it's 2nd transfer and im willing to experiment with CVG cased with jiffy. I was thinking 2 or 3 more transfers? It's my first time with pans and second time growing anything. Are 5 transfers about average for everyone?


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InvisibleTeemo 6T3
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Re: Official Copelandia/Panaeolus Thread [Re: JHOVA]
    #24420115 - 06/20/17 10:23 AM (6 years, 7 months ago)

It depends on how uniform the growth is, sometimes my growth is uniform and clean on the 2nd transfer, sometimes the 4th.

It really depends on what you observe from the myc.

You can transfer a couple of wedges to new agar, and use the one already on agar for experiment, this will ensure that you still have some running on agar if something unfortunate happens :super:


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Shrooming Is Of The Essence


Rest In Peace Dankington


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InvisibleJHOVA
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Re: Official Copelandia/Panaeolus Thread [Re: Teemo 6T3]
    #24420204 - 06/20/17 11:04 AM (6 years, 7 months ago)

Good idea. I hate wasting clean looking plates.:sunny:


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Edited by JHOVA (06/20/17 11:17 AM)


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Offlinehamloaf
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Re: Official Copelandia/Panaeolus Thread [Re: Teemo 6T3]
    #24420210 - 06/20/17 11:06 AM (6 years, 7 months ago)

Quote:

But still, i think your right d0urd3n, cus the first Sandose grow i did, i shook it and it colonised perfectly without any trouble.



Strains, or subsrains aren't going to NOT rejoin everytime.  This is the random factor striking again.

The safest bet I can come up with so far is to take at least five transfers before expanding to grain.  Personally, I plan on going further with the transfers looking for cultures that either rejoin well together on agar, or attempting to catch a single sector isolate.  Would be nice to get some fruits so I can grab some clones.  Panaeolus are some pranksters. 

Another safe bet with Pans is to employ methods that require the least amount of shaking.  Such as like I said earlier LC-----> bag -----> substrate, cakes, or substrate blocks.  Let them colonize the grains without a shake.  Also an advantage of using bags over jars for Pans is that when a shake must be employed to spawn the colonized grains it can be done gently.


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How I Get Stuff done. - My Reference Guide. - My Grows.


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InvisibleJHOVA
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Re: Official Copelandia/Panaeolus Thread [Re: hamloaf]
    #24420227 - 06/20/17 11:16 AM (6 years, 7 months ago)

Il'l keep that in mind, about shaking colonized pans unecessarily Ham.
Is there any difference in starting with smaller jars vs quart jars? Do pans do ok G2G?

Thanks for answering all my questions guys.


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InvisibleTeemo 6T3
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Re: Official Copelandia/Panaeolus Thread [Re: JHOVA] * 1
    #24420551 - 06/20/17 01:32 PM (6 years, 7 months ago)

Here's one of the flower pots, seems like the pins didn't get effected by sprinkling casing on them.



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Shrooming Is Of The Essence


Rest In Peace Dankington


Edited by Teemo 6T3 (06/20/17 01:32 PM)


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Invisibled0urd3n
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Re: Official Copelandia/Panaeolus Thread [Re: Teemo 6T3]
    #24420785 - 06/20/17 02:56 PM (6 years, 7 months ago)

Alright fair enough maybe I just have been lucky then. I will say I've noticed they prefer grain on the drier side. Might give that a shot if you're having trouble. like I said not an expert just something I've noticed. I think that whispy myc doesn't like to cut through water as well.

Throwing bisp, cyan, and cinctulus on grain in 2 weeks once these plates are grown out.


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Offlinehamloaf
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Re: Official Copelandia/Panaeolus Thread [Re: d0urd3n]
    #24420876 - 06/20/17 03:26 PM (6 years, 7 months ago)

Interesting observation, dourden.  That makes some sense.  Cube myce gobbles water up.  Good luck with your next Panaeolus project.


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How I Get Stuff done. - My Reference Guide. - My Grows.


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InvisibleTheMadHatter420
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Re: Official Copelandia/Panaeolus Thread [Re: hamloaf]
    #24421065 - 06/20/17 04:18 PM (6 years, 7 months ago)

Here are 3 of my 4 Pan cambo jars. The date on the jars says I knocked them up on the 9th but I think I may have been tired and put the wrong date down. I did also only use 1/4th of an agar plate per jar. I just don't think it has really been 11 days, fuck maybe it has. O well I will be knocking up some pints of grains later tonight with more Pan cambo.



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Invisiblevan hattonFacebookDiscord
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Re: Official Copelandia/Panaeolus Thread [Re: TheMadHatter420]
    #24421095 - 06/20/17 04:26 PM (6 years, 7 months ago)

Do you have temperature fluctuations​ in your room?


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If I ever give out misinformation please inform me so I can have the correct information. :cheers:

Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.

Caps McGee said:
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InvisibleTheMadHatter420
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Re: Official Copelandia/Panaeolus Thread [Re: van hatton]
    #24421174 - 06/20/17 04:50 PM (6 years, 7 months ago)

Not really, I have everything down in the basement now. Stays rather close to 70f, unless I fire up the dehydrator, then it goes up a couple degrees, I would say over all temp range is maybe 68-75(hot day). Now winter will be a bit different, will have to put a space heater down there as it will be like high 50's low 60's in most of it, a couple nooks will be in the 40's. I got a couple crawlspace like nooks under a couple rooms, lol concrete floor though. I think those spots might work out for cold weather cultures, possibly Psilocybe cyan if I can get spores going again.


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