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ekabpils
Exploring Exotics



Registered: 10/20/21
Posts: 167
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Re: Official Copelandia/Panaeolus Thread [Re: ekabpils] 10
#28626656 - 01/19/24 01:13 AM (8 days, 22 hours ago) |
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Quote:
ekabpils said: After many attempts it seems I will have something ) Mystery SA.

A few more photos:

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Baba Yaga
♥ coir grower

Registered: 09/13/20
Posts: 3,955
Loc: Hyperspace Chicken Coop
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Re: Official Copelandia/Panaeolus Thread [Re: ekabpils] 3
#28626683 - 01/19/24 02:23 AM (8 days, 20 hours ago) |
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In the last two weeks I was at home for 3 days only and left the last bisporus tubs to do their thing. Got mighty hot the last 4 days, when I got back home today the temps in the room where still at 85F at 10pm. All tubs contaminated. Two had a couple of tiny green spots and the third had some white mold and a slime mold which I never had popping up in a tub before.

Harvested the fruits and gave it a good mist to see what is does.
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Reishiman
Hobby grower



Registered: 08/03/23
Posts: 113
Loc: Neither here, nor there
Last seen: 2 hours, 57 minutes
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] 1
#28626743 - 01/19/24 05:05 AM (8 days, 18 hours ago) |
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Quote:
Baba Yaga said: Two had a couple of tiny green spots and the third had some white mold and a slime mold which I never had popping up in a tub before.
Harvested the fruits and gave it a good mist to see what is does.
In this picture is that black stuff on the casing the white mold you're talking about? I think I've had the same thing pop up a few times recently and I'm trying to figure out the source. Do you know a typical source/reason for this contaminant? I'm thinking either bacterial spawn so I'm trying to clean that up, or maybe something they added to either my coco coir or bagged cow manure(which I'm switching to different sources for these materials too. Possibly make few different substrate recipes. Does this sound like much of a plan to troubleshoot this?
Edit: Don't think it's the casing because some of the trays it showed up before I even cased but it was very difficult to see. Started out grayish white and then got darker gray to black especially when misted. Any advice would be appreciated. Thank you.
-------------------- Animals are something invented by plants to move seeds around. ... Terrence McKenna A psychedelic point of view is one that values consciousness... Terrence McKenna
Edited by Reishiman (01/19/24 05:40 AM)
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SwabMarley
Twisted Metal Head



Registered: 12/07/20
Posts: 1,450
Loc: Drunken Stupor
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Re: Official Copelandia/Panaeolus Thread [Re: Reishiman]
#28626816 - 01/19/24 07:33 AM (8 days, 15 hours ago) |
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Some nice work going on here all. Hopefully I’ll have some photos to share of my Pan Natal grow very soon!
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ReverendMyc

Registered: 03/29/19
Posts: 1,494
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] 2
#28626912 - 01/19/24 09:10 AM (8 days, 14 hours ago) |
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Quote:
Baba Yaga said: In the last two weeks I was at home for 3 days only and left the last bisporus tubs to do their thing. Got mighty hot the last 4 days, when I got back home today the temps in the room where still at 85F at 10pm. All tubs contaminated. Two had a couple of tiny green spots and the third had some white mold and a slime mold which I never had popping up in a tub before.

Harvested the fruits and gave it a good mist to see what is does.
 
-------------------- LAGM 2.024Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any moreHow to succeed in mycology (and life) - know nothing, read everything, try something, and accept advice. Don't Panic   
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Tiamo
Trust in LITFA




Registered: 04/07/16
Posts: 1,935
Loc: Amsterdam
Last seen: 12 hours, 38 minutes
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Re: Official Copelandia/Panaeolus Thread [Re: ReverendMyc] 4
#28626931 - 01/19/24 09:32 AM (8 days, 13 hours ago) |
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... He's a little confused, but he got the spirit?
My second time seeing Panaeolus fruits, the first time next to a patch of mold and this time as a weird side pin.
--------------------
If you have used a Miraculix Psilocybin QTest, could you please share your results? Shipping free Ps. natalensis spore prints to any address in The Netherlands, just
Mush love
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AspectOfTheCreator
Mastering the Art of Success



Registered: 12/07/22
Posts: 1,263
Last seen: 2 hours, 30 minutes
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Re: Official Copelandia/Panaeolus Thread [Re: Tiamo]
#28627063 - 01/19/24 11:05 AM (8 days, 12 hours ago) |
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Pretty sure theres a few people here getting good results supplementing coir rather than using manure. What are these people using?
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stagger



Registered: 05/05/19
Posts: 1,292
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I wouldn't say I've had great results, but I've had some results using coir only on a few grows this past month. These were ttbvi, straw/manure on left, coir on the right
 And tropicalis, coir only, cased with verm/pumice
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Baba Yaga
♥ coir grower

Registered: 09/13/20
Posts: 3,955
Loc: Hyperspace Chicken Coop
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Re: Official Copelandia/Panaeolus Thread [Re: Bra]
#28627094 - 01/19/24 11:37 AM (8 days, 11 hours ago) |
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Doesn't seem like it made a real difference with those ttbvi.
The tropicalis are looking great!
@aspectotthecreator
look into sleepydave's journal where he talks about his supplementation.
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SwabMarley
Twisted Metal Head



Registered: 12/07/20
Posts: 1,450
Loc: Drunken Stupor
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Re: Official Copelandia/Panaeolus Thread [Re: stagger]
#28627102 - 01/19/24 11:41 AM (8 days, 11 hours ago) |
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What’s the potency like on those TTBVI compared to standard BVI? I done 0.7G of some of my BVI’s yesterday (first time trying them) and they fuckin blew my head off    In a good way though.
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RockinRobot
Stranger


Registered: 12/08/22
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Last seen: 4 hours, 11 minutes
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Re: Official Copelandia/Panaeolus Thread [Re: SwabMarley] 3
#28627116 - 01/19/24 11:51 AM (8 days, 11 hours ago) |
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Starting to get excited. Sent these 4 TTBVI plates to Grain today



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Baba Yaga
♥ coir grower

Registered: 09/13/20
Posts: 3,955
Loc: Hyperspace Chicken Coop
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Re: Official Copelandia/Panaeolus Thread [Re: Reishiman]
#28627153 - 01/19/24 12:16 PM (8 days, 11 hours ago) |
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Doesn't seem like it made a real difference with those ttbvi.
The tropicalis are looking great. Bra had also good success growing tropicalis on just coir. Maybe it's the species.
Quote:
Reishiman said:
Quote:
Baba Yaga said: Two had a couple of tiny green spots and the third had some white mold and a slime mold which I never had popping up in a tub before.
Harvested the fruits and gave it a good mist to see what is does.
In this picture is that black stuff on the casing the white mold you're talking about? I think I've had the same thing pop up a few times recently and I'm trying to figure out the source. Do you know a typical source/reason for this contaminant? I'm thinking either bacterial spawn so I'm trying to clean that up, or maybe something they added to either my coco coir or bagged cow manure(which I'm switching to different sources for these materials too. Possibly make few different substrate recipes. Does this sound like much of a plan to troubleshoot this?
Edit: Don't think it's the casing because some of the trays it showed up before I even cased but it was very difficult to see. Started out grayish white and then got darker gray to black especially when misted. Any advice would be appreciated. Thank you.
The black to the left are spores and what I called white mold is right above the black patch in the top left corner. I don't know, looking at the photo again I would say that the fluffy white is just a different form of the slime mold as they do seem to be connected. This morning, 10 hours later, almost all of the white mold has disappeared and what is left now are a few little brown things which are the fruit bodies, they do look like spore pods.

These tubs are now 6 weeks since spawn so I don't see it being related to what you are experiencing in regards to contamination. Sounds either like your spawn or maybe you are pasteurizing at too high temperatures for too long. Switching sources seems like one way to go about it to rule everything out and make sure you follow a pasteurization TEK as well. JakeOnCid's long pasteurization at 162F (or 165F ???) worked well for me for a couple of years or try the liquefied pasteurization I do if you haven't. Any higher temperatures than that is not necessary imo.
I can't recall seeing photos of your spawn or the mold you are talking about, got some photos or links?
Edited by Baba Yaga (01/19/24 12:49 PM)
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THE MYCOSMITH
Weekend mycologist


Registered: 01/24/22
Posts: 171
Last seen: 8 hours, 32 minutes
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] 5
#28627246 - 01/19/24 01:30 PM (8 days, 9 hours ago) |
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Hey Ekabpils good to see you still play with it and got some mature fruits of the mystery SA. Is that a grow of a fruit clone culture or ms grow. I did a clon grow newly that that I postet in the pic of the day thread for 2 weeks ago.
Mystery S.A



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pablotx
pipette monkey


Registered: 05/06/23
Posts: 45
Loc: Hot & humid
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Re: Official Copelandia/Panaeolus Thread [Re: stagger]
#28627300 - 01/19/24 02:13 PM (8 days, 9 hours ago) |
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Quote:
stagger said: I wouldn't say I've had great results, but I've had some results using coir only on a few grows this past month. These were ttbvi, straw/manure on left, coir on the right

The one on the right is grain spawn + coir for bulk? Is it cased? Sorry if this is a dumb question, but I've yet to get Pans to fruit from pan cakes or in a monotub. Frustration is kicking in.
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stagger



Registered: 05/05/19
Posts: 1,292
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Re: Official Copelandia/Panaeolus Thread [Re: pablotx] 2
#28627388 - 01/19/24 03:30 PM (8 days, 7 hours ago) |
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pablotx, yes that tray is grain spawn (wbs) and coir. Cased with pasteurized 50/50 peat/verm.
@swab, hopefully I get a chance to sit down properly with them this weekend. I agree about the traditional bvi also, I was a bit surprised at 1.2g. peace river surprised me the same way. Bisporus has been the least aggressive of the few pan I've tried, but still my favorite I think. You guys articulate your experiences very well and I won't even try to ruin it with my words, but i really felt what tree frog had described about them. Maybe that wasn't in this thread, I forget exactly.
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Reishiman
Hobby grower



Registered: 08/03/23
Posts: 113
Loc: Neither here, nor there
Last seen: 2 hours, 57 minutes
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
#28627402 - 01/19/24 03:44 PM (8 days, 7 hours ago) |
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Quote:
Baba Yaga said: Doesn't seem like it made a real difference with those ttbvi.
The tropicalis are looking great. Bra had also good success growing tropicalis on just coir. Maybe it's the species.
Quote:
Reishiman said:
Quote:
Baba Yaga said: Two had a couple of tiny green spots and the third had some white mold and a slime mold which I never had popping up in a tub before.
Harvested the fruits and gave it a good mist to see what is does.
In this picture is that black stuff on the casing the white mold you're talking about? I think I've had the same thing pop up a few times recently and I'm trying to figure out the source. Do you know a typical source/reason for this contaminant? I'm thinking either bacterial spawn so I'm trying to clean that up, or maybe something they added to either my coco coir or bagged cow manure(which I'm switching to different sources for these materials too. Possibly make few different substrate recipes. Does this sound like much of a plan to troubleshoot this?
Edit: Don't think it's the casing because some of the trays it showed up before I even cased but it was very difficult to see. Started out grayish white and then got darker gray to black especially when misted. Any advice would be appreciated. Thank you.
The black to the left are spores and what I called white mold is right above the black patch in the top left corner. I don't know, looking at the photo again I would say that the fluffy white is just a different form of the slime mold as they do seem to be connected. This morning, 10 hours later, almost all of the white mold has disappeared and what is left now are a few little brown things which are the fruit bodies, they do look like spore pods.

These tubs are now 6 weeks since spawn so I don't see it being related to what you are experiencing in regards to contamination. Sounds either like your spawn or maybe you are pasteurizing at too high temperatures for too long. Switching sources seems like one way to go about it to rule everything out and make sure you follow a pasteurization TEK as well. JakeOnCid's long pasteurization at 162F (or 165F ???) worked well for me for a couple of years or try the liquefied pasteurization I do if you haven't. Any higher temperatures than that is not necessary imo.
I can't recall seeing photos of your spawn or the mold you are talking about, got some photos or links?
Sorry but didn't take pictures of the spawn jars. They colonized in under a week and bounced right back after a shake halfway through that week. I thought I was either growing mold or had some really fast pan mycelium. In fact they looked so good that I prepared the bulk right before full colonization without doing a sniff test first (wanted to keep a good momentum going) So when I spawned, every single jar had a slightly sweet smell when first opened. Then I shook the jars to break up and when I smelled them again they all smelt like clean mycelium. No hint of sweetness after breaking up?Either way my bulk was prepared so I went ahead and spawned it. But I had basically lost most of my hopes at this point. This is an example of how it went for most of the trays.
 You can see it almost all colonized.(getting my hopes up again) but one area is very under colonized an I noticed some bur like growth on a piece of straw. and some grayish mold started to form on the surface of the under colonized area and this is pretty much how all the trays went, one at a time the mold or bacteria got a foot hold. Like 10 trays. The trays that didn't mold I continued to monitor. They kept getting metabolites and what looked like possibly wet bubble and even some clear jelly like substance where some of the mycelium was poking through the casing surface. Possibly from getting too wet. Also I didn't notice any odor while they were in the fc. Sometimes I wouldn't smell anything and sometimes got a faint smell of mushrooms but I think it should have smelt more like mushrooms. Like I said no pics of the spawn but here I'm going back to the same culture and transfered to water agar to try and outrun any possible parasitic bacteria. This was transferred 3 days ago
 I'm also trying a different grain prep. I was doing 75% millet 25% rye and soaking for 24 hours. Now I'm trying a different method of just soaking rye berries in the instant pot on the keep warm setting (150 degrees about) Soaked like that for 4 hours and then rinsed in cold water and left to dry for about 12 hours. Testing this prep method on Mexicana as we speak, before I try it on the pans. So far it looks good. And the grains actually came out looking more hydrated than with the 24 hour soak and there was no fermented smell like my usual grain prep method would cause before sterilizing. Here's a picture of the Mexicana on grain inoculated 3 days ago (sorry about it being on this thread) but I plan on using the same preparation for pans. And also plan on trying this with just millet to rule out millet being the source of my problems.(hopefully not because I just bought a 50 lbs bag of it

About the pasteurizing tek. I have tried that long pasteurizing process with my instant pot. But like I said the keep warm setting on mine only reaches 150. When I used this method for cubes I had pasteurized a manure based substrate for 7 or 8 hours and it worked. Only thing is I didn't let it sit for 5 days on a shelf to build the microbial count(haven't seen anyone else mention doing this so I didn't want to risk it.) So I'll do some side by sides for the next run: your liquid method (will have to look up the details again), the long jakeoncid method, and also my usual method (basically what rr does except I use gallon ziplock bags instead of jars.) And I will try for temperatures not exceeding 160 for 2 hours. Thank you for the help. Will update this thread with my progress.
-------------------- Animals are something invented by plants to move seeds around. ... Terrence McKenna A psychedelic point of view is one that values consciousness... Terrence McKenna
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Baba Yaga
♥ coir grower

Registered: 09/13/20
Posts: 3,955
Loc: Hyperspace Chicken Coop
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Re: Official Copelandia/Panaeolus Thread [Re: Reishiman]
#28627428 - 01/19/24 04:08 PM (8 days, 7 hours ago) |
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Pasteurizing @ 150F in the instapot sounds actually like a good setup and it worked so why change anything? Letting things sit for a while is probably only beneficial for pasteurization which happened at a higher temperatures to let the microbes recover from getting too hot.
I actually remember seeing your post showing the substrate with the bald spot. I noticed that the lids you had on those trays had a lot of condensation on them. Maybe the bold spot is where the water kept dripping onto the substrate making it soaking wet in that area which could have caused or contributed to your problems. Usually if things don't fruit or abort easily I suspect a mold causing the problems.
Would be good to have another look at your cultures once they are going back to regular agar. BRF-pucks are also a great way to clean things up.
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old_fart_out_west
Stranger


Registered: 01/27/23 
Posts: 49
Last seen: 2 hours, 32 minutes
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
#28627443 - 01/19/24 04:15 PM (8 days, 7 hours ago) |
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Had a couple questions about growing pans in marthas.
Current setup:
- Fog is piped into the top
- Tub of water with an aquarium heater is placed on the bottom shelf, temperature set to 85
- Tent is unzipped up to the first shelf
- There is no exhaust fan
- A space heater is placed about 4' away and blows hot air at the tent
Questions are: 1. Will this setup provide have enough fresh air exchange? 2. Does it matter if I run my fogger in cycles of 20 min on / 40 min off vs 5 min / 10 min off (both equating to 20 minutes of fog per hour)?
Edited by old_fart_out_west (01/19/24 04:15 PM)
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3.A.M
Boop!



Registered: 10/17/22
Posts: 847
Loc: Oz
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Re: Official Copelandia/Panaeolus Thread [Re: SwabMarley] 2
#28627476 - 01/19/24 04:39 PM (8 days, 6 hours ago) |
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Quote:
SwabMarley said: What’s the potency like on those TTBVI compared to standard BVI? I done 0.7G of some of my BVI’s yesterday (first time trying them) and they fuckin blew my head off    In a good way though.
Not sure about standard BVI but I had .5 last night and was well impressed, was like having 1.5g ghost but the visuals were just a bit better, definitely my new favourite go to variety.
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Reishiman
Hobby grower



Registered: 08/03/23
Posts: 113
Loc: Neither here, nor there
Last seen: 2 hours, 57 minutes
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] 1
#28627504 - 01/19/24 05:07 PM (8 days, 6 hours ago) |
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Quote:
Baba Yaga said: Pasteurizing @ 150F in the instapot sounds actually like a good setup and it worked so why change anything? Letting things sit for a while is probably only beneficial for pasteurization which happened at a higher temperatures to let the microbes recover from getting too hot.
I actually remember seeing your post showing the substrate with the bald spot. I noticed that the lids you had on those trays had a lot of condensation on them. Maybe the bold spot is where the water kept dripping onto the substrate making it soaking wet in that area which could have caused or contributed to your problems. Usually if things don't fruit or abort easily I suspect a mold causing the problems.
Would be good to have another look at your cultures once they are going back to regular agar. BRF-pucks are also a great way to clean things up.
Ok. I think I'm on the right track now thanks. Yeah I'm not planning to use those aluminium trays anymore because the metabolites seem to dissolve holes through it. Was probably going to try just aluminum foil cover like rr recommends, on plastic or glass trays. Also want to try a mono tub or 2 like what you do. Maybe I'll have better luck with that. Will update when I get the culture back on regular agar.And yes Should probably make up a few brf pucks to inoculate the next time I inoculate grain spawn (or B4) That way if the grain spawn fails I'll already have that growing on brf pucks. Thanks again for the help. Much appreciated.
-------------------- Animals are something invented by plants to move seeds around. ... Terrence McKenna A psychedelic point of view is one that values consciousness... Terrence McKenna
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