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OfflinePanarchist
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Registered: 04/02/16
Posts: 432
Loc: Copeland
Last seen: 7 months, 16 days
Re: Official Copelandia/Panaeolus Thread [Re: tryptkaloids]
    #24297899 - 05/05/17 03:52 PM (6 years, 8 months ago)

funkymonk22, good luck with your project. i will be curious to see how they turn out.


tryptkaloids, good luck with yours as well. if the mycelium was able to colonize a significant enough portion of the growth medium you may get some fruits.


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Offlinehamloaf
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Re: Official Copelandia/Panaeolus Thread [Re: Panarchist] * 1
    #24309227 - 05/10/17 09:32 AM (6 years, 8 months ago)

Made a syringe out of an old print labeled "pan cyan Tampa".
Had a quart jar that was sterilized about 4 days before being inoculated.
The jar was for another project, but was forgotten about.
Ran across the jar a few days after making the syringe.
For kicks, and giggles I shot some a spores into the jar.
This is the result 5 days from inoculation. 
 


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How I Get Stuff done. - My Reference Guide. - My Grows.


Edited by hamloaf (05/10/17 09:58 AM)


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OfflinePsiloPsychIn
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Re: Official Copelandia/Panaeolus Thread [Re: hamloaf]
    #24309295 - 05/10/17 09:57 AM (6 years, 8 months ago)

Lookin Good Ham!

(nice oat prep too!)


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OfflineSpeed Racer
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Registered: 04/28/17
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Re: Official Copelandia/Panaeolus Thread [Re: PsiloPsychIn]
    #24309436 - 05/10/17 11:02 AM (6 years, 8 months ago)

Do these plates look good to you? First generation from a spore syringe inoculated 8 days ago. Panaeolus Cyanescens from the spore depot.

I'm thinking of doing my first transfer. Do I have some good material to transfer?







Edited by Speed Racer (05/10/17 02:05 PM)


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InvisibleTheMadHatter420
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Registered: 10/12/16
Posts: 12,941
Re: Official Copelandia/Panaeolus Thread [Re: Speed Racer]
    #24309457 - 05/10/17 11:10 AM (6 years, 8 months ago)

STOP POSTING VENDOR NAMES.


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InvisibleMr.Mouse
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Re: Official Copelandia/Panaeolus Thread [Re: Speed Racer]
    #24309494 - 05/10/17 11:25 AM (6 years, 8 months ago)

And my best guess is that you have an infection of papulospora byssina (I'm not an expert though and would wait for some further opinions) .  Are those pre-poured plates?


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OfflineSpeed Racer
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Re: Official Copelandia/Panaeolus Thread [Re: Mr.Mouse]
    #24309517 - 05/10/17 11:33 AM (6 years, 8 months ago)

Pre poured potato dextros.


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OfflineOrganic_Magic
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Re: Official Copelandia/Panaeolus Thread [Re: Speed Racer]
    #24309527 - 05/10/17 11:38 AM (6 years, 8 months ago)

So I've got a bispora print that I'm itching to get on agar and while I've read some trip reports of pans Im really  wanting a good comparison between cubes and pans. I know they are drastically more potent, but what about the "essence" of the trip itself. I'm wanting a different trip than my average cube experience really.


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OfflinePanarchist
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Re: Official Copelandia/Panaeolus Thread [Re: TheMadHatter420]
    #24309893 - 05/10/17 01:52 PM (6 years, 8 months ago)

Speed Racer, be aware that we are not supposed to mention using spores from vendors for anything other than microscopy purposes. i would suggest you edit your post to remove that line. and it's a little hard to see from your pictures but it looks like you have some decent transferable growth to work with. in general you want to look for where the growth is most advanced, least disorganized and most robust. and if you observe them from the underside you may see areas where the mycelium is more white and free of darkened blotchy areas than others, this is a good sign. in my opinion, the best areas to transfer from on the plates you have are:

C plate: on the northwest colony anywhere from 7:00 to 11:00

H plate: 7:00 to 9:00 on the most well developed colony

E plate: 8:00 to 10:00 and 3:00 on the southern colony
        1:00 to 3:00 on the southeastern colony.

I plate: 7:00 to 4:00 on the northern colony.




Organic_Magic, i've only done cubes once and it was a long time ago so i can't give a good comparison between them and pans but in comparing pans to other psychedelics a couple things stood out to me.
one was that they had a much more mellow vibe. there was non of the stimulant-like effects other psychedelics tend to have, it was very chilled out. another thing was the complete elimination of any tension or anxiety that can happen. i was really feeling no worries.
and there was no stomach upset whatsoever. and the visuals were quite nice as well, more geometric and not so abstract as i hear cubes can be.


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Invisibled0urd3n
Just call me "D"

Registered: 09/15/10
Posts: 5,237
Re: Official Copelandia/Panaeolus Thread [Re: Panarchist]
    #24309936 - 05/10/17 02:06 PM (6 years, 8 months ago)

What dosage are you referring to?


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Invisiblemynakedrat
The phantom hourglass
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Registered: 02/16/17
Posts: 4,629
Loc: Inner Astral levels Flag
Re: Official Copelandia/Panaeolus Thread [Re: Panarchist]
    #24309943 - 05/10/17 02:10 PM (6 years, 8 months ago)

Quote:

Panarchist said:
Speed Racer, be aware that we are not supposed to mention using spores from vendors for anything other than microscopy purposes. i would suggest you edit your post to remove that line. and it's a little hard to see from your pictures but it looks like you have some decent transferable growth to work with. in general you want to look for where the growth is most advanced, least disorganized and most robust. and if you observe them from the underside you may see areas where the mycelium is more white and free of darkened blotchy areas than others, this is a good sign. in my opinion, the best areas to transfer from on the plates you have are:

C plate: on the northwest colony anywhere from 7:00 to 11:00

H plate: 7:00 to 9:00 on the most well developed colony

E plate: 8:00 to 10:00 and 3:00 on the southern colony
        1:00 to 3:00 on the southeastern colony.

I plate: 7:00 to 4:00 on the northern colony.




Organic_Magic, i've only done cubes once and it was a long time ago so i can't give a good comparison between them and pans but in comparing pans to other psychedelics a couple things stood out to me.
one was that they had a much more mellow vibe. there was non of the stimulant-like effects other psychedelics tend to have, it was very chilled out. another thing was the complete elimination of any tension or anxiety that can happen. i was really feeling no worries.
and there was no stomach upset whatsoever. and the visuals were quite nice as well, more geometric and not so abstract as i hear cubes can be.





There's little black dots all over his fuzz


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OfflineSpeed Racer
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Registered: 04/28/17
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Re: Official Copelandia/Panaeolus Thread [Re: mynakedrat]
    #24309958 - 05/10/17 02:16 PM (6 years, 8 months ago)

Thanks Panarchist, that is encouraging. Edit Done.

The spore syringe does have some visible black dots floating around in it. Not thick maybe 1%.


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OfflinePanarchist
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Re: Official Copelandia/Panaeolus Thread [Re: Panarchist]
    #24309986 - 05/10/17 02:28 PM (6 years, 8 months ago)

d0urd3n, 6.75 grams of fresh pan tropicalis.

mynakedrat, can you point out where they are? i am not seeing them even after zooming in on the picture.

Speed Racer, good luck with your project. and i would assume those black dots are spores.


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OfflineSpeed Racer
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Registered: 04/28/17
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Re: Official Copelandia/Panaeolus Thread [Re: Panarchist]
    #24310031 - 05/10/17 02:47 PM (6 years, 8 months ago)

Panarchist, thanks for you help.

mynakedrat, when i do the transfer I will look closely and avoid any black dots.

I'll look at the top and bottom of the plates and mark the bottom with a sharpie where to take samples from before I go into the SAB.

This forum is extremely helpful and quite robust. Once you get some experience it is very easy to post pictures and navigate around.

Thanks Moderators...and everyone else involved in this experience.


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Invisiblemynakedrat
The phantom hourglass
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Registered: 02/16/17
Posts: 4,629
Loc: Inner Astral levels Flag
Re: Official Copelandia/Panaeolus Thread [Re: Panarchist]
    #24310120 - 05/10/17 03:32 PM (6 years, 8 months ago)

Quote:

Panarchist said:
d0urd3n, 6.75 grams of fresh pan tropicalis.

mynakedrat, can you point out where they are? i am not seeing them even after zooming in on the picture.

Speed Racer, good luck with your project. and i would assume those black dots are spores.



Picture 2, center of myc growth and a little on the edges. Pi ture 3 on the edges and past. Little spore packets. White mold, not myc anywhere I see. Thays all fuzz.


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InvisibleTheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,941
Re: Official Copelandia/Panaeolus Thread [Re: mynakedrat]
    #24310206 - 05/10/17 04:15 PM (6 years, 8 months ago)

I started Pan cambo, Pan bi, and Pan Cyan on agar within the last 24 hours. I would like to try to grow the 3 side by side for visual reference and fun. Why the fuck not right? lol


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OfflinePanarchist
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Re: Official Copelandia/Panaeolus Thread [Re: Panarchist]
    #24310271 - 05/10/17 04:47 PM (6 years, 8 months ago)

mynakedrat, thanks for clarifying, i was mostly looking at the picture with all the plates and didn't scrutinize the others as much. while i would agree that the black spots are something to be avoided i don't think that means that all of these plates are unusable and i don't think that the growth on these plates is only mold mycelium. those plates look on par with what i've seen from 8 days of pan mycelium growth. i had some plates a little while back that were infected with the mold with white mycelium that sporulates black and the growth was far more advanced in a shorter length of time. this was them at 6 days.



only time will tell for sure though so Speed Racer please keep us updated on your results.

TheMadHatter420, good luck with your project and please keep us updated on your results too. i have only worked with pan cyan and pan tropicalis mycelium so far but i have noticed some distinct differences between them. i will be curious to see what you find.


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InvisibleTheMadHatter420
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Registered: 10/12/16
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Re: Official Copelandia/Panaeolus Thread [Re: Panarchist]
    #24310279 - 05/10/17 04:49 PM (6 years, 8 months ago)

I'm curious as well. Only taken cubes to the end so far.


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OfflineSpeed Racer
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Re: Official Copelandia/Panaeolus Thread [Re: TheMadHatter420]
    #24310419 - 05/10/17 05:49 PM (6 years, 8 months ago)

What do you think about Plates A, B & F?

They look clean and very uniform to me.  I don't know what is with the darker center.

I have a sleeve of 10 pre poured plates and I'll use them all on this transfer this evening. For shits and giggles I'll label them showing which plate they came from.

Later...got to go scrub up.


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OfflinePanarchist
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Re: Official Copelandia/Panaeolus Thread [Re: Speed Racer]
    #24310556 - 05/10/17 06:39 PM (6 years, 8 months ago)

though they show some uniformity, the growth on plates A and B looks kind of weak and faint and lacking in other signs that would indicate healthiness. the growth on plate F looks robust on those outer edges but that darkening in the middle is so well developed that i would expect that the infection is already spread into the robust-looking areas but just hasn't revealed itself yet. observing the way pan mycelium reacts to infection can offer insight but i wouldn't use those plates for anything else.


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