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SwabMarley
Twisted Metal Head



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Re: Official Copelandia/Panaeolus Thread [Re: fraki58]
#28569455 - 12/05/23 08:03 AM (1 month, 24 days ago) |
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Thanks man. There are a few more scattered around so may just wait for the pinset and select the biggest of a cluster I think!
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tree frog
eats bugs


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Re: Official Copelandia/Panaeolus Thread [Re: SwabMarley]
#28569473 - 12/05/23 08:15 AM (1 month, 24 days ago) |
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Are Bisporus really slow to fruit or should I give up on these two little bags I cased? They've been in fruiting since day before Thanksgiving. So three weeks next Thursday.
I've looked at some pictures of knots and I don't think they're knotting.
-------------------- Listen to the silence behind the engines' noise. Jesus, Sweets, listen. Hear it? It's a love song. For whom? You are loved. ~ David Foster Wallace, Westward the Course of Empire Takes Its Way
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stagger



Registered: 05/05/19
Posts: 1,294
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Re: Official Copelandia/Panaeolus Thread [Re: tree frog] 1
#28569541 - 12/05/23 09:04 AM (1 month, 24 days ago) |
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You're not even at 2 weeks yet in this current week, why'd you mention 3 weeks next Thursday lol? Have some patience tree frog, even 3 weeks has been mentioned here as normal to wait for pins.
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Snazz
Polymath



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Loc: Canada
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Re: Official Copelandia/Panaeolus Thread [Re: tree frog] 2
#28569579 - 12/05/23 09:30 AM (1 month, 24 days ago) |
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Quote:
tree frog said: Are Bisporus really slow to fruit or should I give up on these two little bags I cased? They've been in fruiting since day before Thanksgiving. So three weeks next Thursday.
I've looked at some pictures of knots and I don't think they're knotting.
Pan Bi are very slow to mature, but they will keep flushing like mad once established
As long as you keep the sub moist
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tree frog
eats bugs


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Re: Official Copelandia/Panaeolus Thread [Re: stagger]
#28569619 - 12/05/23 09:57 AM (1 month, 24 days ago) |
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Quote:
stagger said: You're not even at 2 weeks yet in this current week, why'd you mention 3 weeks next Thursday lol? Have some patience tree frog, even 3 weeks has been mentioned here as normal to wait for pins.
lol okay I apparently thought Thanksgiving was the Thursday before Thanksgiving. I even looked at a calendar before posting just not good with tracking days or holidays.
Either way I appreciate both of your responses.
edit: also I confuse this and next when it comes to dates. my gf literally pointed this out to me like two days ago.
-------------------- Listen to the silence behind the engines' noise. Jesus, Sweets, listen. Hear it? It's a love song. For whom? You are loved. ~ David Foster Wallace, Westward the Course of Empire Takes Its Way
Edited by tree frog (12/05/23 09:59 AM)
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SwabMarley
Twisted Metal Head



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Re: Official Copelandia/Panaeolus Thread [Re: tree frog] 1
#28569633 - 12/05/23 10:04 AM (1 month, 24 days ago) |
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In my experience it takes 10-14 days to colonise the surface and then another 10-14 after casing. They are definitely slower than other varieties of pans I’ve grown. Thats why I call them the APE’s of Pans. Potency checks out as well!
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SwabMarley
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Re: Official Copelandia/Panaeolus Thread [Re: SwabMarley] 9
#28570899 - 12/06/23 03:10 AM (1 month, 23 days ago) |
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Quote:
SwabMarley said: First little BVI pins. Shite pic but first time with these so 

Same tub 12 hours later!

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Bismillah
Scharf im Schlafanzug


Registered: 07/11/22
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Re: Official Copelandia/Panaeolus Thread [Re: SwabMarley] 1
#28571388 - 12/06/23 10:56 AM (1 month, 23 days ago) |
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This is my second go at pasteurized bulk, 1:1 hpoo and coir. Been colonizing for 2 weeks.
This one looks ok, perhaps something iffy in the lower left corner. Nocced grain from SSLC.

This is probably not good. Nocced from agar.

Spawn was nocced up using the black plate:

Jar on the day of spawning:

Toss it?
Edited by Bismillah (12/06/23 10:58 AM)
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Baba Yaga
♥ coir grower

Registered: 09/13/20
Posts: 3,958
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Re: Official Copelandia/Panaeolus Thread [Re: Bismillah] 1
#28571585 - 12/06/23 01:49 PM (1 month, 23 days ago) |
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2 weeks is a long time for complete colonization and consolidation with a normal pan at average room temperature. Did it take that long or did you just let is sit for that long? Maybe it was a bit wet?
Nah, I'd say you've come that far, case and fruit won't cost much more.
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Bismillah
Scharf im Schlafanzug


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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
#28571605 - 12/06/23 02:08 PM (1 month, 23 days ago) |
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Interesting, so those brown blobs are not much of a concern? I guess if anything this is bacterial that won't spread to the other tray.
My FC is full of trays that are still producing so I figured these trays could wait. Is it bad to let them colonize too long?
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Baba Yaga
♥ coir grower

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Re: Official Copelandia/Panaeolus Thread [Re: Bismillah] 2
#28571645 - 12/06/23 02:43 PM (1 month, 23 days ago) |
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The longer you wait the more yellow they produce IME. When I let them consolidate for a bit longer they are starting to excreted amber droplets, I think that these are just metabolites not necessarily related to a bacterial problems as I had not an ill effect which I could directly relate to this occurrence.
These are two tubs I let go for a bit longer:

I guess if I had a lot of water sloshing around on the surface as it is on your tray it could end up looking similar. That is a lot of water, though I think it's not as detrimental when it accumulated after full colonization. I personally would give it a go just to find out.
Edited by Baba Yaga (12/06/23 02:58 PM)
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Bismillah
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
#28572313 - 12/07/23 02:21 AM (1 month, 22 days ago) |
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Thanks Baba. I could also stick a tray in an unmodified tub. Lid upside down, slight gap, mist when pins appear -- is that still the recommended practice? Cased or uncased?
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Baba Yaga
♥ coir grower

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Re: Official Copelandia/Panaeolus Thread [Re: Bismillah] 1
#28572339 - 12/07/23 03:27 AM (1 month, 22 days ago) |
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Yes, that is one way to do it. I would pour off the excess water and case it since it is already consolidating for a while. Now I'm getting curious to see how this is going to end.
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Tiamo
Trust in LITFA




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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] 7
#28572364 - 12/07/23 04:26 AM (1 month, 22 days ago) |
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Pans smell amazing and it's funny how the smell changes before and after shaking the spawn.
I pray my substrate is properly pasteurized and my spawn was clean enough because I got a big old tub on the way.
--------------------
If you have used a Miraculix Psilocybin QTest, could you please share your results? Shipping free Ps. natalensis spore prints to any address in The Netherlands, just
Mush love
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Baba Yaga
♥ coir grower

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Re: Official Copelandia/Panaeolus Thread [Re: Tiamo] 3
#28572799 - 12/07/23 12:34 PM (1 month, 22 days ago) |
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Pans do smell so much better than cubes. A few days ago I dried only a hand full of cubes for the first time in 2 years. It was like driving a herd of goats through the house........totally forgot how pungent their odor is.
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Baba Yaga
♥ coir grower

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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] 5
#28572871 - 12/07/23 01:30 PM (1 month, 22 days ago) |
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I documented my shake regime and what it often looks like when I give pans a late shake. This is RDU (left) and Bisporus (right).
As you can see on the first image I use grain flour/Vermiculite substrate on top of my grain as is does help with recovery and gives a lot of robust inoculation points which do recover very fast after a shake and the jars don't "stall" either. I also like to see the mycelium grow out on top as it gives me another chance to look out for contamination. For more explanation on this see here.
From first shake after inoculation to second shake. Photos were taken before shake, after 1 day, 2 days and 3.5 days.
 
From day two after second shake to almost full colonization. Photos were taken 2 days after second shake and 3, 4 and 8 days after.

This sequence of photos are showing jars from the same batch which were shaken at 98% colonization and their recovery. Photos were taken before the shake, 3 days after and the last three images are from 4 days after.

As you can see the late shake caused a lot of condensation in the jar which made the recovery look weak but these are jars which I would spawn and not be thinking twice about it as they were looking absolutely fine before the late shake. I would just let them sit for a few days to a week and spawn. They usually do recover just like any other colonized pan spawn without issues. I think that this phenomenon has something to do with the fast growth speed of panaeolus and how a lot of colonized grain recovering at the same time can produce heat and vapor which does create the wet look of the spawn. External factors like room temp and temp swings several degrees during recovery seem to exacerbate this phenomenon.
I haven't grown these out yet but I have previously documented how jars like that turn out just fine in the post quote below.
Quote:
Baba Yaga said: Final update on the poorly recovered jars:
So just to recap I spawned these two jars which wouldn't recover growing out nice and fluffy but looked like this which often happens for me when shaking a jar that is almost done.
Two weeks after last shake:

I was throwing jars like this out at the beginning but after a few of them I questioned whether this was actually contamination as there were no signs of any contam before the shake, no mold, no creamy dense mycelium, no syrupy wet kernels or suspect looking bald spots. Yes they do recover poorly and they look wet after the shake but there is a difference to bacterial wet and condensation wet IMO. The final push to give them a go was reading a statement of member conicr saying that this happens sometimes but its no deal breaker. Now I spawn jars like this whenever I get them and have no problems. Also sitting in the jar for 2 weeks doesn't seem to make a difference. I still do throw jars out though just not when totally fine almost done jars recover poorly after a shake.
Both tubs received FAE during colonization and were put into full fruiting condition one day after full colonization. Temps plummeted for a couple of nights while fruiting and went down to 68F in the room, I've got the heater going now.
First clone C4T3 which was a day behind but turned out to be an OK tub. This is also the least productive clone out of the 6 I got going.
236g wet. 1 myco quart @ a spawn ratio of ~1:2.5

Second clone C3T3, pretty good and I used the other style tub for this which I like only downside is you can't see through the plastic.
327g wet. 1 myco quart @ a spawn ratio of ~1:3.5

If you have a jar like this and there was no sign of contamination before the shake then try spawning it. You might be surprised and I sincerely hope it will be in a good way.
Edit: update photo and weight
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SwabMarley
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] 7
#28573769 - 12/08/23 12:46 AM (1 month, 21 days ago) |
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Nice findings baba. I’ve given some of my jars 2 shakes this time and it actually seems to have helped a bit as after the second shake they seem to have gone pretty crazy!
Harvested some BVI yesterday. Just an MS. Didn’t manage to get any clones but will be on the look out for a cluster on the second flush.
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Bismillah
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Re: Official Copelandia/Panaeolus Thread [Re: SwabMarley] 4
#28573864 - 12/08/23 05:10 AM (1 month, 21 days ago) |
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So yesterday I cleaned out my tub. The water was brown, slime all over. I also did some modifications, so the process took perhaps 30-40 minutes. I'm startled to see that today the caps are all cracked and pins have aborted. Are pans really that sensitive to temp/humidity swings? The room is 7C at the moment but I put the trays in my hotbox with the fan off while cleaning. Temp in there went from 25 to 15. Then I filled the tub with 7C water, stuck a 50W aquarium heater in set at 30C, and after about 1.5h the FC was back at 28C and 90% RH.
Tray on the left, before cleaning. I picked the biggest ones.

Tray today:

I'm running without an ultrasonic now too, but I still have the fan that kicks on for 5s every minute. This fan used to distribute the fog. I've had some nice flushes before this though so I don't think it's the fan/lack of fog that did this.
Edited by Bismillah (12/08/23 05:11 AM)
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Genome794
Spore-Whore-der & Agar-Addict


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Re: Official Copelandia/Panaeolus Thread [Re: Bismillah] 2
#28574422 - 12/08/23 01:36 PM (1 month, 21 days ago) |
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@Bismillah and SwabMarley Mighty fine grows there.
Finally arriving with Pans... Sub is CVG with approx 20% composted hpoo (and some horse bedding... Its what I have). Spawn sub ratio 1:30. Light compression. 3x Dub-tub (2 x 4L units). Pan Cyan Jamaica, fully colonised in 4 days.

Cased with a sprinkle of 50/50+

Left this one to be the uncased side-by-side. To see what they like.

Very happy to finally be getting somewhere with pans. I am glad to have a few good cultures.
Thanks to everyone who has chimed in along my journey while I was struggling with a difficult/bad culture.
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Telomere
Unravelling


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Re: Official Copelandia/Panaeolus Thread [Re: Genome794] 16
#28574476 - 12/08/23 02:19 PM (1 month, 21 days ago) |
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Here’s some more pics of these sporeless Florida King Pans
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