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the_chosen_one
On the Darkslide


Registered: 09/11/06
Posts: 2,885
Loc: 1984
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Re: Official Copelandia/Panaeolus Thread [Re: YoshiTrainer] 2
#28555911 - 11/25/23 10:28 AM (2 months, 3 days ago) |
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Quote:
YoshiTrainer said: @TOC, worm poo works too with CV or of course, straw. π
It's your fault damn it. Where did you get this thing? It's nuts. Shreds agar too fast.
Got all that but running low on worm atm thanks to the ladies. I did find an old bag of dried horse poo and I have some steer manure for gardening in the garage now that I think of it. Might find some road apples when I take the dog for a walk. I guess I have some time. I'll probably put it to grain this weekend or next. Or just T4. 
You're all awesome! I'll try and update. Thanks!
-------------------- "Luck favors the observant." - Workman
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YoshiTrainer
Onion tied to belt



Registered: 04/30/22
Posts: 1,209
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Re: Official Copelandia/Panaeolus Thread [Re: the_chosen_one] 2
#28555977 - 11/25/23 12:07 PM (2 months, 3 days ago) |
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Quote:
the_chosen_one said: It's your fault damn it. Where did you get this thing? It's nuts. Shreds agar too fast.

I got it from a friend of the Devil. π
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rockyfungus
dirty


Registered: 03/01/21
Posts: 1,062
Loc: Front range
Last seen: 21 days, 5 hours
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Re: Official Copelandia/Panaeolus Thread [Re: YoshiTrainer] 2
#28556039 - 11/25/23 01:33 PM (2 months, 3 days ago) |
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Quote:
YoshiTrainer said:
Quote:
the_chosen_one said: It's your fault damn it. Where did you get this thing? It's nuts. Shreds agar too fast.

I got it from a friend of the Devil. π
Probably a trash can
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Adas
Lonely Dreamer



Registered: 12/22/16
Posts: 5,270
Loc: Central EU
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Re: Official Copelandia/Panaeolus Thread [Re: tree frog] 2
#28556659 - 11/26/23 02:01 AM (2 months, 2 days ago) |
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Quote:
tree frog said: meanwhile, I can't get Bisporus to run well on grains. Adding manure to everything just to get it going.
This is my go-to procedure if I want fast and vigorous colonization of spawn. Even just pure sawdust works for this. Maybe they like the cellulose.
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Argyreia
Grafting cacti is awesome



Registered: 11/19/14
Posts: 1,100
Loc: France
Last seen: 1 month, 20 days
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Re: Official Copelandia/Panaeolus Thread [Re: Adas] 1
#28556685 - 11/26/23 03:32 AM (2 months, 2 days ago) |
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I tried again after my half failure. It did fruit but not mature well and there wasn't even enough to feel something. This time out of 4 cakes, one of them went wild with that cotton layer on top and big boys. Did everything the same tho... Don't know what is going on with p. cyan.

What I may have done better is more humidity and more hemp in the sub.
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tree frog
eats bugs


Registered: 09/14/23
Posts: 458
Loc: lives in trees
Last seen: 2 hours, 4 minutes
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Re: Official Copelandia/Panaeolus Thread [Re: Adas] 3
#28556755 - 11/26/23 06:08 AM (2 months, 2 days ago) |
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Quote:
Adas said:
Quote:
tree frog said: meanwhile, I can't get Bisporus to run well on grains. Adding manure to everything just to get it going.
This is my go-to procedure if I want fast and vigorous colonization of spawn. Even just pure sawdust works for this. Maybe they like the cellulose.
I've been soaking my millet in manure tea and that did help some. Right now I have some LCs running on millet/rye and just millet, both soaked in manure and it hasn't stalled anyway. Also agar to millet soaked in manure tea is going, as well as millet with the pinches of manure added in. The LCs have been faster than the wedges to millet + poo (rather the poo is added directly or via compost soak). And caught up to and have surpassed the jars inoculated with wedges even though they're a week older. So yeah, not sure what they're lacking from grains.
I'll try some sadwdust, thanks for the suggestion. Probably run some side by sides with sawdust and sawdust + manure tea (I like the tea just so I don't have black shit in my jars. Plus it has run as well as adding straight manure so far for me).
edit
Here's some comparison pictures from oldest projects to newest. Agar to millet all stalled so severely the jars have been resterilized for oyster mushroom projects.
Manure added to jar, wedge inoculation. Shaken four days ago.

Manure tea, wedge inoculation. Shaken four days ago. Looks close to finished but the camera isn't picking up the fainter colonization.

Straight to substrate, wedge. Colonized and will case this morning. Two other bags already cased and in fruiting. Inoculated after the two jars above. Millet, manure, coir, and straw.

LC on millet soaked in compost tea. Shaken two days ago (third, shake so these will finish soon).

LC on millet and rye (compost tea and gypsum, think I skipped gypsum in my other jars). Recovering from second shake morning before yesterday.

-LC jars were shaken once at first signs of recovery and once more after a few days to distribute moisture (had a 60cc LC syringe so the jars got blasted with LC and are pretty moist). First LC jar (third jar pictured with all the moisture visible at the top) is on third and final shake.
I'll post again once the jars start to finish. But for my culture at least going wedge to anything other than bags or LCs has taken many weeks. And the non-poo millet jars didn't run at all.
-------------------- Listen to the silence behind the engines' noise. Jesus, Sweets, listen. Hear it? It's a love song. For whom? You are loved. ~ David Foster Wallace, Westward the Course of Empire Takes Its Way
Edited by tree frog (11/26/23 06:43 AM)
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Skropi
Stranger

Registered: 11/01/23
Posts: 190
Loc: Greece
Last seen: 5 hours, 41 minutes
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Re: Official Copelandia/Panaeolus Thread [Re: tree frog]
#28556797 - 11/26/23 07:12 AM (2 months, 2 days ago) |
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A few minutes ago I started two clones. I am 99% sure that I did something wrong, in the whole procedure, but certainly, that 1% of me actually getting a nice culture, is filling me with hope! I also just sterilised 4 half pints, and I will inoculate them tonight with B+. I figured, better have two projects at once, better chances at success I am just not sure how to go about the pan cyans, if/when I get a clean culture on agar. Go the grain way, or follow this one https://www.shroomery.org/forums/showflat.php/Number/18357343#18357343 ?
-------------------- αΌΞ½Ξ΄ΟΞ± ΞΌΞΏΞΉ αΌΞ½Ξ½Ξ΅ΟΞ΅, ΞΞΏαΏ¦ΟΞ±, ΟΞΏΞ»ΟΟΟΞΏΟΞΏΞ½
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tree frog
eats bugs


Registered: 09/14/23
Posts: 458
Loc: lives in trees
Last seen: 2 hours, 4 minutes
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Re: Official Copelandia/Panaeolus Thread [Re: Skropi]
#28556812 - 11/26/23 07:33 AM (2 months, 2 days ago) |
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Well getting Bisporus to grow on grain has been problematic for people. If you look at my last post you'll see my successes and failures (well the total failures aren't posted but four agar to millet jars stalled completely).
Cyans maybe a different story.
-------------------- Listen to the silence behind the engines' noise. Jesus, Sweets, listen. Hear it? It's a love song. For whom? You are loved. ~ David Foster Wallace, Westward the Course of Empire Takes Its Way
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SupaThaRipper
Genetics Hoarder



Registered: 09/02/13
Posts: 1,510
Loc: USA
Last seen: 42 minutes, 31 seconds
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Re: Official Copelandia/Panaeolus Thread [Re: tree frog]
#28556817 - 11/26/23 07:41 AM (2 months, 2 days ago) |
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Quote:
tree frog said: Well getting Bisporus to grow on grain has been problematic for people. If you look at my last post you'll see my successes and failures (well the total failures aren't posted but four agar to millet jars stalled completely).
Cyans maybe a different story.
Iβm glad I made more LC right away. Do you know what variety you have?
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tree frog
eats bugs


Registered: 09/14/23
Posts: 458
Loc: lives in trees
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Re: Official Copelandia/Panaeolus Thread [Re: SupaThaRipper]
#28556824 - 11/26/23 07:49 AM (2 months, 2 days ago) |
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Just Pan Bisporus. Got it from a well regarded sponsor. I'll send you a pm with a link but there's no strain listed, just area the original print was acquired.
-------------------- Listen to the silence behind the engines' noise. Jesus, Sweets, listen. Hear it? It's a love song. For whom? You are loved. ~ David Foster Wallace, Westward the Course of Empire Takes Its Way
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Skropi
Stranger

Registered: 11/01/23
Posts: 190
Loc: Greece
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Re: Official Copelandia/Panaeolus Thread [Re: tree frog]
#28556971 - 11/26/23 09:49 AM (2 months, 2 days ago) |
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I don't know guys, but this experience I had with pan cyans, the grow kit from Zamnesia to be xact, was perfect, it exceeded every expectation I had, so I will put more weight on growing these. Cubes will be more like a hobby
-------------------- αΌΞ½Ξ΄ΟΞ± ΞΌΞΏΞΉ αΌΞ½Ξ½Ξ΅ΟΞ΅, ΞΞΏαΏ¦ΟΞ±, ΟΞΏΞ»ΟΟΟΞΏΟΞΏΞ½
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SwabMarley
Twisted Metal Head



Registered: 12/07/20
Posts: 1,450
Loc: Drunken Stupor
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Re: Official Copelandia/Panaeolus Thread [Re: Skropi] 9
#28556982 - 11/26/23 09:56 AM (2 months, 2 days ago) |
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Contrast of Bispo myc to BVI myc on grain
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Blueberry Muffin
Stranger


Registered: 09/15/23
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Re: Official Copelandia/Panaeolus Thread [Re: SwabMarley] 6
#28557050 - 11/26/23 10:58 AM (2 months, 2 days ago) |
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My first (non-side) pins ever. Ttbvi half pint hpoo brf verm fahtbag.
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MojaveMyc
Fractal Farmer


Registered: 05/08/21
Posts: 660
Loc: Colorado
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Re: Official Copelandia/Panaeolus Thread [Re: SwabMarley]
#28557062 - 11/26/23 11:15 AM (2 months, 2 days ago) |
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Quote:
SwabMarley said: Contrast of Bispo myc to BVI myc on grain

Would you consider that BVI good to go? Asking because I have a Jambo culture that looks similar on grain but have been hesitant to spawn it. Pan mycβs still kinda foreign to me
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SupaThaRipper
Genetics Hoarder



Registered: 09/02/13
Posts: 1,510
Loc: USA
Last seen: 42 minutes, 31 seconds
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Quote:
theodor said:

My first (non-side) pins ever. Ttbvi half pint hpoo brf verm fahtbag.
π€
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Blueberry Muffin
Stranger


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Re: Official Copelandia/Panaeolus Thread [Re: SupaThaRipper]
#28557143 - 11/26/23 12:38 PM (2 months, 2 days ago) |
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Quote:
SupaThaRipper said:
Quote:
theodor said:

My first (non-side) pins ever. Ttbvi half pint hpoo brf verm fahtbag.
π€
What's so funny
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Baba Yaga
β₯ coir grower

Registered: 09/13/20
Posts: 3,958
Loc: Hyperspace Chicken Coop
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Re: Official Copelandia/Panaeolus Thread [Re: tree frog] 3
#28557183 - 11/26/23 01:02 PM (2 months, 2 days ago) |
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Quote:
tree frog said: Well getting Bisporus to grow on grain has been problematic for people. If you look at my last post you'll see my successes and failures (well the total failures aren't posted but four agar to millet jars stalled completely).
Cyans maybe a different story.
Pans always took ages to take on grain for me regardless whether it was a wedge or LC. Don't know why but once they have overcome the first hump they would be doing alright. That is why I am using whole wheat flour/verm on top of pan jars. It's like the PF recipe but only half the amount of flour (2:1:1/2). They grow fine on it so I don't think it is a deficiency issue.
This is RDU on the left and Bisporus on the right. I shook them today and will update on the progress.

Took a RDU pin straight to BRF puck.

and spawned two RDU jars to supplemented coir/verm using liquid seaweed compost. 50 - 90 ml to 10 liters of water are recommended depending on soil quality. Forgot to account for the lesser water amount and used 50ml to 3.5 liters of water, ooooooops.
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Baba Yaga
β₯ coir grower

Registered: 09/13/20
Posts: 3,958
Loc: Hyperspace Chicken Coop
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Quote:
theodor said:
Quote:
SupaThaRipper said:
Quote:
theodor said:

My first (non-side) pins ever. Ttbvi half pint hpoo brf verm fahtbag.
π€
What's so funny 
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tree frog
eats bugs


Registered: 09/14/23
Posts: 458
Loc: lives in trees
Last seen: 2 hours, 4 minutes
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
#28557253 - 11/26/23 01:49 PM (2 months, 2 days ago) |
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It might be a moisture issue as they like the flour/verm layer.
The LC jars that are running well I put 5ccs in each.
And they like tons of moisture to fruit right?
-------------------- Listen to the silence behind the engines' noise. Jesus, Sweets, listen. Hear it? It's a love song. For whom? You are loved. ~ David Foster Wallace, Westward the Course of Empire Takes Its Way
Edited by tree frog (11/26/23 02:02 PM)
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SupaThaRipper
Genetics Hoarder



Registered: 09/02/13
Posts: 1,510
Loc: USA
Last seen: 42 minutes, 31 seconds
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Quote:
theodor said:
Quote:
SupaThaRipper said:
Quote:
theodor said:

My first (non-side) pins ever. Ttbvi half pint hpoo brf verm fahtbag.
π€
What's so funny 
Thatβs not laughter π€£ thatβs excitement! Iβm happy for you!
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